LOCUS       Exported                7608 bp ds-DNA     circular GCC 23-AUG-2018
DEFINITION  pMRE121-PnptII-mTurquoise2-Hyun.
ACCESSION   .
VERSION     .
KEYWORDS    pMRE131_pntpII_mTurqouise2
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2018 from SnapGene 4.0.8
            http://www.snapgene.com
COMMENT     This file was created using Genome Compiler.
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism=""
     rep_origin      1023..1792
                     /locus_tag=""
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid p
                     BBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep
                     protein for replication"
     CDS             1793..2455
                     /codon_start=1
                     /locus_tag=""
                     /product="Rep"
                     /label=rep
                     /note="required for plasmid replication;Geneious name: rep 
                     CDS"
                     /db_xref="11093880"
                     /protein_id="AAG29471.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     2437..2446
                     /label=FWD_primer_PnptII
     primer_bind     2443..2462
                     /label=pMRE130_cassette1_FWD
     primer_bind     2443..2462
                     /label=pGRG36_MRE13X_cassette_FW
     primer_bind     complement(2566..2586)
                     /label=Rev_Tet
     primer_bind     complement(2567..2585)
                     /label=pMRE11-6/7/8/9rev
     primer_bind     2630..2649
                     /label=pMRE130_cassette3_FWD
     primer_bind     2630..2649
                     /label=cassette3_pGRG36overhang_FWD
     primer_bind     2915..2930
                     /label=Chloramp_rev
     primer_bind     3020..3039
                     /label=pMRE130_cassette4_FWD
     primer_bind     3020..3039
                     /label=cassette4_pGRG36overhang_FWD
     primer_bind     3146..3169
                     /label=pMRE130_cassette2_FWD
     primer_bind     3146..3169
                     /label=pMRE130_fwd_new
     primer_bind     3148..3163
                     /label=tn5_insert_FWD
     primer_bind     3162..3182
                     /label=pMRE130inserts_fwd
     primer_bind     3162..3178
                     /locus_tag=""
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar va
                     riants"
     primer_bind     3162..3178
                     /label=M13F
     promoter        3188..3206
                     /locus_tag=""
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3239..3240
                     /locus_tag=""
                     /product=""
                     /label=T1 rrnB terminator region
                     /note="T1 rrnB terminator region;Geneious name: T1 rrnB ter
                     minator region regulatory"
     terminator      3249..3335
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3432..3518
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3615..3701
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3796..3882
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     primer_bind     3942..3967
                     /label=cassette5_pGRG36overhang_FWD
     primer_bind     3946..3971
                     /label=cassette_pAG408overhang_FWD
     primer_bind     3948..3973
                     /locus_tag=""
                     /label=Geneious name: MCS primer
                     /note="Geneious name: MCS primer"
     primer_bind     3948..3973
                     /label=pFru97 MCS fw
     primer_bind     3948..3968
                     /label=REV_pFru_ins
     primer_bind     3958..3994
                     /label=FWD_primer_PnptII
     promoter        3985..4320
                     /locus_tag=""
                     /label=p-nptII promoter
                     /label=p-nptII
     primer_bind     4134..4152
                     /label=pFru97xpMRE100_nptIIGib_rv
     primer_bind     complement(4319..4349)
                     /label=REV_primer_PnptII_mTq2/sG2
     primer_bind     complement(4319..4339)
                     /label=REV_primer_PnptII_mCl/mO/mSc/sY2
     primer_bind     complement(4319..4339)
                     /label=REV_primer_PnptII_mTagBFP2
     primer_bind     4329..4359
                     /label=FWD_primer_mTq2/sGFP2
     primer_bind     4329..4356
                     /label=FWD_primer_mCl/mO/mSc/sY2
     CDS             4341..5053
                     /codon_start=1
                     /locus_tag=""
                     /product=""
                     /label=mTurquoise2
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     4341..4356
                     /label=FWD_sYFP2
     primer_bind     4341..4354
                     /label=XFPs for
     primer_bind     complement(4858..4878)
                     /label=FWD_primer_PnptII
     primer_bind     complement(5004..5073)
                     /label=REV_primer_sG2
     primer_bind     complement(5034..5073)
                     /label=REV_primer_mTq2
     primer_bind     complement(5035..5057)
                     /label=NotI_XFP_rev
     primer_bind     complement(5035..5056)
                     /label=nextgenFP.rv
     primer_bind     complement(5037..5073)
                     /label=REV_primer_mO
     primer_bind     complement(5037..5056)
                     /label=11x-FL-rv
     primer_bind     complement(5038..5073)
                     /label=REV_primer_sY2
     primer_bind     complement(5038..5056)
                     /label=REV_sYFP2
     primer_bind     complement(5038..5056)
                     /label=Ptac+eXFP_rev
     primer_bind     complement(5039..5056)
                     /label=XFPs rev KpnI
     primer_bind     5054..5068
                     /label=Gib_Gent.rev
     primer_bind     complement(5061..5080)
                     /label=FWD_pFru_ins
     primer_bind     5064..5086
                     /label=Gib_Gent.for.cor
     terminator      5070..5164
                     /locus_tag=""
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             5208..5867
                     /codon_start=1
                     /locus_tag=""
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5269..5297)
                     /label=pMRE130inserts_rev
     primer_bind     complement(5852..5867)
                     /label=Chloramp_rev
     terminator      5932..6018
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     primer_bind     complement(6023..6040)
                     /label=Gib_Gent.for
     primer_bind     complement(6028..6045)
                     /label=pMRE130_cassette2_RV
     primer_bind     complement(6029..6045)
                     /label=cassette_pAG408overhang_RV
     primer_bind     complement(6040..6062)
                     /label=tn5_insert_REV
     primer_bind     complement(6241..6260)
                     /label=pMRE130_cassette3_RV
     primer_bind     complement(6241..6260)
                     /label=cassette3_pGRG36overhang_RV
     primer_bind     complement(6330..6350)
                     /label=cassette4_pGRG36overhang_RV
     primer_bind     complement(6330..6349)
                     /label=pMRE130_cassette4_RV
     primer_bind     complement(6352..6371)
                     /label=pMRE130_cassette1_RV
     primer_bind     complement(6352..6371)
                     /label=pGRG36_MRE13X_cassette_RV
     CDS             complement(6357..7151)
                     /codon_start=1
                     /locus_tag=""
                     /product="kanamycin phosphotransferase"
                     /label=npt
                     /note="kanamycin resistance determinant;Geneious name: npt 
                     CDS"
                     /db_xref="11093881"
                     /protein_id="AAG29472.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     7148..7169
                     /label=pFru97xpMRE100_nptIIGib_fw
     primer_bind     7149..7169
                     /label=pFru97xpMRE101/102nptII_r
     primer_bind     complement(7333..7351)
                     /label=pFru97xpMRE100_nptIIGib_rv
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctg gggccgcaat tcccaattcc
     3241 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3301 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3361 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat
     3421 tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3481 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     3541 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt
     3601 aattccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     3661 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     3721 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccagga
     3781 attaattcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3841 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3901 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3961 aattggggat cggattcgag ctcgcacgct gccgcagcac tcagggcgca agggctgcta
     4021 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     4081 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     4141 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     4201 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     4261 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggata
     4321 ctagtggagg aagaaaaaaa tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4381 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4441 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4501 cgtgccctgg cccaccctcg tgaccaccct gtcctggggc gtgcagtgct tcgcccgcta
     4561 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4621 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4681 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4741 caacatcctg gggcacaagc tggagtacaa ctactttagc gacaacgtct atatcaccgc
     4801 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg
     4861 cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4921 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     4981 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5041 cgagctgtac aagtaattag ctgagcttgg actcctgttg atagatccag taatgacctc
     5101 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
     5161 gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     5221 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     5281 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     5341 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     5401 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
     5461 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     5521 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     5581 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     5641 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     5701 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     5761 tgccgctggc gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc
     5821 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
     5881 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
     5941 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     6001 ctcctgagta ggacaaatcc gccgctagga gcttgcggcc gccagtgtga tggatatctg
     6061 cataaaaact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     6121 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     6181 atgggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     6241 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     6301 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     6361 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     6421 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     6481 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     6541 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     6601 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     6661 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     6721 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     6781 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     6841 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     6901 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     6961 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     7021 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     7081 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     7141 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc
     7201 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     7261 ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct
     7321 agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc
     7381 cttgtccaga tagcccagta gctgacattc atcccaggtg gcacttttcg gggaaatgtg
     7441 cgcgcccgcg ttcctgctgg cgctgggcct gtttctggcg ctggacttcc cgctgttccg
     7501 tcagcagctt ttcgcccacg gccttgatga tcgcggcggc cttggcctgc atatcccgat
     7561 tcaacggccc cagggcgtcc agaacgggct tcaggcgctc ccgaaggt
//
