LOCUS       Exported                7612 bp ds-DNA     circular GCC 23-AUG-2018
DEFINITION  pMRE123-PnptII-sYFP2-Hyun.
ACCESSION   .
VERSION     .
KEYWORDS    pMRE133_pnptII_sYFP2
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2018 from SnapGene 4.0.8
            http://www.snapgene.com
COMMENT     This file was created using Genome Compiler.
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism=""
     rep_origin      1023..1792
                     /locus_tag=""
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid p
                     BBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep
                     protein for replication"
     CDS             1793..2455
                     /codon_start=1
                     /locus_tag=""
                     /product="Rep"
                     /label=rep
                     /note="required for plasmid replication;Geneious name: rep 
                     CDS"
                     /db_xref="11093880"
                     /protein_id="AAG29471.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     2437..2446
                     /label=FWD_primer_PnptII
     primer_bind     2443..2462
                     /label=pMRE130_cassette1_FWD
     primer_bind     2443..2462
                     /label=pGRG36_MRE13X_cassette_FW
     primer_bind     complement(2566..2586)
                     /label=Rev_Tet
     primer_bind     complement(2567..2585)
                     /label=pMRE11-6/7/8/9rev
     primer_bind     2630..2649
                     /label=pMRE130_cassette3_FWD
     primer_bind     2630..2649
                     /label=cassette3_pGRG36overhang_FWD
     primer_bind     2915..2930
                     /label=Chloramp_rev
     primer_bind     3020..3039
                     /label=pMRE130_cassette4_FWD
     primer_bind     3020..3039
                     /label=cassette4_pGRG36overhang_FWD
     primer_bind     3146..3169
                     /label=pMRE130_cassette2_FWD
     primer_bind     3146..3169
                     /label=pMRE130_fwd_new
     primer_bind     3148..3163
                     /label=tn5_insert_FWD
     primer_bind     3162..3182
                     /label=pMRE130inserts_fwd
     primer_bind     3162..3178
                     /locus_tag=""
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar va
                     riants"
     primer_bind     3162..3178
                     /label=M13F
     promoter        3188..3206
                     /locus_tag=""
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3239..3240
                     /locus_tag=""
                     /product=""
                     /label=T1 rrnB terminator region
                     /note="T1 rrnB terminator region;Geneious name: T1 rrnB ter
                     minator region regulatory"
     terminator      3249..3335
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3432..3518
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3615..3701
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3796..3882
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     primer_bind     3942..3967
                     /label=cassette5_pGRG36overhang_FWD
     primer_bind     3946..3971
                     /label=cassette_pAG408overhang_FWD
     primer_bind     3948..3973
                     /locus_tag=""
                     /label=Geneious name: MCS primer
                     /note="Geneious name: MCS primer"
     primer_bind     3948..3973
                     /label=pFru97 MCS fw
     primer_bind     3948..3968
                     /label=REV_pFru_ins
     primer_bind     3958..3994
                     /label=FWD_primer_PnptII
     promoter        3985..4321
                     /locus_tag=""
                     /label=p-nptII promoter
                     /label=p-nptII
     primer_bind     3985..4004
                     /label=11x-nptII-FL-fw
     primer_bind     3985..3999
                     /label=lederm_nptII.fw
     primer_bind     complement(4134..4158)
                     /label=pntpII_1_REV
     primer_bind     4134..4158
                     /label=pntpII_1_FWD
     primer_bind     4135..4153
                     /label=pFru97xpMRE100_nptIIGib_rv
     primer_bind     complement(4256..4276)
                     /label=pntpII_2_REV
     primer_bind     4256..4276
                     /label=pntpII_2_FWD
     primer_bind     complement(4300..4334)
                     /label=rbs_1_REV
     primer_bind     4300..4334
                     /label=rbs_1_FWD
     primer_bind     complement(4320..4349)
                     /label=REV_primer_PnptII_mTq2/sG2
     primer_bind     complement(4320..4342)
                     /label=REV_primer_PnptII_mCl/mO/mSc/sY2
     primer_bind     complement(4320..4342)
                     /label=REV_primer_PnptII_mTagBFP2
     primer_bind     4330..4359
                     /label=FWD_primer_mTq2/sGFP2
     primer_bind     4330..4356
                     /label=FWD_primer_mCl/mO/mSc/sY2
     CDS             4340..5057
                     /codon_start=1
                     /locus_tag=""
                     /product=""
                     /label=sYFP2
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     4341..4356
                     /label=FWD_sYFP2
     primer_bind     4341..4354
                     /label=XFPs for
     primer_bind     4341..4352
                     /label=XhoI_Ptac_XFP_for
     primer_bind     complement(4858..4878)
                     /label=FWD_primer_PnptII
     primer_bind     complement(5004..5023)
                     /label=REV_primer_sG2
     primer_bind     complement(5034..5077)
                     /label=REV_primer_mTq2
     primer_bind     complement(5035..5057)
                     /label=nextgenFP.rv
     primer_bind     complement(5035..5056)
                     /label=NotI_XFP_rev
     primer_bind     complement(5037..5077)
                     /label=REV_primer_mO
     primer_bind     complement(5037..5056)
                     /label=11x-FL-rv
     primer_bind     complement(5038..5077)
                     /label=REV_primer_sY2
     primer_bind     complement(5038..5057)
                     /label=REV_sYFP2
     primer_bind     complement(5038..5057)
                     /label=Ptac+eXFP_rev
     primer_bind     complement(5039..5057)
                     /label=XFPs rev KpnI
     primer_bind     complement(5065..5084)
                     /label=FWD_pFru_ins
     primer_bind     5068..5090
                     /label=Gib_Gent.for.cor
     terminator      5074..5168
                     /locus_tag=""
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             5212..5871
                     /codon_start=1
                     /locus_tag=""
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5273..5301)
                     /label=pMRE130inserts_rev
     primer_bind     complement(5856..5871)
                     /label=Chloramp_rev
     terminator      5936..6022
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     primer_bind     complement(6027..6044)
                     /label=Gib_Gent.for
     primer_bind     complement(6032..6049)
                     /label=cassette_pGRG36overhang_RV
     primer_bind     complement(6032..6049)
                     /label=pMRE130_cassette2_RV
     primer_bind     complement(6033..6049)
                     /label=cassette_pAG408overhang_RV
     primer_bind     complement(6044..6066)
                     /label=tn5_insert_REV
     primer_bind     complement(6245..6264)
                     /label=pMRE130_cassette3_RV
     primer_bind     complement(6245..6264)
                     /label=cassette3_pGRG36overhang_RV
     primer_bind     complement(6334..6354)
                     /label=cassette4_pGRG36overhang_RV
     primer_bind     complement(6334..6353)
                     /label=pMRE130_cassette4_RV
     primer_bind     complement(6356..6375)
                     /label=pMRE130_cassette1_RV
     primer_bind     complement(6356..6375)
                     /label=pGRG36_MRE13X_cassette_RV
     CDS             complement(6361..7155)
                     /codon_start=1
                     /locus_tag=""
                     /product="kanamycin phosphotransferase"
                     /label=npt
                     /note="kanamycin resistance determinant;Geneious name: npt 
                     CDS"
                     /db_xref="11093881"
                     /protein_id="AAG29472.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     7152..7173
                     /label=pFru97xpMRE100_nptIIGib_fw
     primer_bind     7153..7173
                     /label=pFru97xpMRE101/102nptII_r
     primer_bind     7170..7190
                     /label=rbs_1_REV
     primer_bind     7214..7234
                     /label=pntpII_2_REV
     primer_bind     complement(7214..7234)
                     /label=pntpII_2_FWD
     primer_bind     7332..7356
                     /label=pntpII_1_REV
     primer_bind     complement(7332..7356)
                     /label=pntpII_1_FWD
     primer_bind     complement(7337..7355)
                     /label=pFru97xpMRE100_nptIIGib_rv
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctg gggccgcaat tcccaattcc
     3241 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3301 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3361 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat
     3421 tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3481 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     3541 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt
     3601 aattccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     3661 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     3721 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccagga
     3781 attaattcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3841 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3901 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3961 aattggggat cggattcgag ctcgcacgct gccgcaagca ctcagggcgc aagggctgct
     4021 aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     4081 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     4141 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     4201 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     4261 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
     4321 actagtggag gaagaaaaaa tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4381 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4441 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4501 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcgcccgcta
     4561 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4621 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4681 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4741 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc
     4801 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg
     4861 cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4921 gctgcccgac aaccactacc tgagctacca gtccaagctg agcaaagacc ccaacgagaa
     4981 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5041 cgagctgtac aagtaagtaa ttagctgagc ttggactcct gttgatagat ccagtaatga
     5101 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
     5161 gtgagaatcc aagctagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa
     5221 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     5281 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     5341 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     5401 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
     5461 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     5521 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     5581 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     5641 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     5701 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     5761 ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga
     5821 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa
     5881 ggcagttatt ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat
     5941 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     6001 cgctctcctg agtaggacaa atccgccgct aggagcttgc ggccgccagt gtgatggata
     6061 tctgcataaa aactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     6121 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     6181 gcccatgggg gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
     6241 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
     6301 aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
     6361 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     6421 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     6481 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     6541 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     6601 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     6661 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     6721 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     6781 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     6841 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     6901 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     6961 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     7021 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     7081 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     7141 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct
     7201 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
     7261 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca
     7321 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt
     7381 ttcccttgtc cagatagccc agtagctgac attcatccca ggtggcactt ttcggggaaa
     7441 tgtgcgcgcc cgcgttcctg ctggcgctgg gcctgtttct ggcgctggac ttcccgctgt
     7501 tccgtcagca gcttttcgcc cacggccttg atgatcgcgg cggccttggc ctgcatatcc
     7561 cgattcaacg gccccagggc gtccagaacg ggcttcaggc gctcccgaag gt
//
