LOCUS       Exported                8404 bp ds-DNA     circular SYN 29-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   EJPARKER
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2018 from SnapGene 4.0.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1023..1792
                     /locus_tag=""
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid p
                     BBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep
                     protein for replication"
     CDS             1793..2455
                     /codon_start=1
                     /locus_tag=""
                     /product="Rep"
                     /label=rep
                     /note="required for plasmid replication;Geneious name: rep 
                     CDS"
                     /db_xref="11093880"
                     /protein_id="AAG29471.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     2437..2446
                     /label=FWD_primer_PnptII
     primer_bind     2443..2462
                     /label=pGRG36_MRE13X_cassette_FW
     primer_bind     2443..2462
                     /label=pMRE130_cassette1_FWD
     primer_bind     complement(2566..2586)
                     /label=Rev_Tet
     primer_bind     complement(2567..2585)
                     /label=pMRE11-6/7/8/9rev
     primer_bind     2630..2649
                     /label=cassette3_pGRG36overhang_FWD
     primer_bind     2630..2649
                     /label=pMRE130_cassette3_FWD
     primer_bind     2915..2930
                     /label=Chloramp_rev
     primer_bind     3020..3039
                     /label=cassette4_pGRG36overhang_FWD
     primer_bind     3020..3039
                     /label=pMRE130_cassette4_FWD
     primer_bind     3146..3169
                     /label=pMRE130_fwd_new
     primer_bind     3146..3169
                     /label=pMRE130_cassette2_FWD
     primer_bind     3148..3163
                     /label=tn5_insert_FWD
     primer_bind     3162..3182
                     /label=pMRE130inserts_fwd
     primer_bind     3162..3178
                     /locus_tag=""
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar va
                     riants"
     primer_bind     3162..3178
                     /label=M13F
     promoter        3188..3206
                     /locus_tag=""
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3239..3240
                     /locus_tag=""
                     /product=""
                     /label=T1 rrnB terminator region
                     /note="T1 rrnB terminator region;Geneious name: T1 rrnB ter
                     minator region regulatory"
     terminator      3249..3335
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3432..3518
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3615..3701
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3796..3882
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     primer_bind     3942..3967
                     /label=cassette5_pGRG36overhang_FWD
     primer_bind     3946..3971
                     /label=cassette_pAG408overhang_FWD
     primer_bind     3948..3973
                     /locus_tag=""
                     /label=Geneious name: MCS primer
                     /note="Geneious name: MCS primer"
     primer_bind     3948..3973
                     /label=pFru97 MCS fw
     primer_bind     3948..3968
                     /label=REV_pFru_ins
     primer_bind     3966..4002
                     /label=71_FWD_Gent
     promoter        4042..4070
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     4042..4070
                     /label=pmre116.for
     primer_bind     4091..4113
                     /label=FWD_Tri
     CDS             4259..4792
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     complement(4259..4276)
                     /label=pAG-GmR-rev
     primer_bind     complement(4278..4291)
                     /label=pAG-GmR-rev
     primer_bind     4769..4786
                     /label=pAG408_rainbowFOR
     primer_bind     complement(4773..4805)
                     /label=72_RV_Gent
     primer_bind     complement(4773..4792)
                     /label=Gent.rev
     primer_bind     complement(4773..4792)
                     /label=pmre113.for
     primer_bind     complement(4775..4792)
                     /label=NotI_GmR_rev
     primer_bind     4793..4808
                     /label=FWD_primer_PnptII
     promoter        4799..5135
                     /locus_tag=""
                     /label=p-nptII promoter
                     /label=p-nptII
     primer_bind     4799..4818
                     /label=11x-nptII-FL-fw
     primer_bind     4799..4813
                     /label=lederm_nptII.fw
     primer_bind     4949..4967
                     /label=pFru97xpMRE100_nptIIGib_rv
     primer_bind     complement(5134..5166)
                     /label=REV_primer_PnptII_mTq2/sG2
     primer_bind     complement(5134..5163)
                     /label=REV_primer_PnptII_mCl/mO/mSc/sY2
     primer_bind     complement(5134..5159)
                     /label=REV_primer_PnptII_mTagBFP2
     primer_bind     5152..5169
                     /label=XhoI_Ptac_XFP_for
     CDS             5154..5849
                     /codon_start=1
                     /locus_tag=""
                     /product=""
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     5154..5171
                     /label=XFPs for
     primer_bind     complement(5154..5171)
                     /label=Ptuf rev
     primer_bind     complement(5830..5869)
                     /label=REV_primer_mTq2
     primer_bind     complement(5831..5853)
                     /label=NotI_XFP_rev
     primer_bind     complement(5831..5852)
                     /label=nextgenFP.rv
     primer_bind     complement(5833..5869)
                     /label=REV_primer_mO
     primer_bind     complement(5833..5852)
                     /label=11x-FL-rv
     primer_bind     complement(5834..5869)
                     /label=REV_primer_sY2
     primer_bind     complement(5834..5852)
                     /label=REV_sYFP2
     primer_bind     complement(5834..5852)
                     /label=Ptac+eXFP_rev
     primer_bind     complement(5835..5852)
                     /label=XFPs rev KpnI
     primer_bind     5850..5864
                     /label=Gib_Gent.rev
     primer_bind     complement(5857..5876)
                     /label=FWD_pFru_ins
     primer_bind     5860..5882
                     /label=Gib_Gent.for.cor
     terminator      5866..5960
                     /locus_tag=""
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             6004..6663
                     /codon_start=1
                     /locus_tag=""
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6065..6093)
                     /label=pMRE130inserts_rev
     primer_bind     complement(6648..6663)
                     /label=Chloramp_rev
     terminator      6728..6814
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     primer_bind     complement(6819..6836)
                     /label=Gib_Gent.for
     primer_bind     complement(6824..6841)
                     /label=pMRE130_cassette2_RV
     primer_bind     complement(6825..6841)
                     /label=cassette_pAG408overhang_RV
     primer_bind     complement(6836..6858)
                     /label=tn5_insert_REV
     primer_bind     complement(7037..7056)
                     /label=cassette3_pGRG36overhang_RV
     primer_bind     complement(7037..7056)
                     /label=pMRE130_cassette3_RV
     primer_bind     complement(7126..7146)
                     /label=cassette4_pGRG36overhang_RV
     primer_bind     complement(7126..7145)
                     /label=pMRE130_cassette4_RV
     primer_bind     complement(7148..7167)
                     /label=pGRG36_MRE13X_cassette_RV
     primer_bind     complement(7148..7167)
                     /label=pMRE130_cassette1_RV
     CDS             complement(7153..7947)
                     /codon_start=1
                     /locus_tag=""
                     /product="kanamycin phosphotransferase"
                     /label=npt
                     /note="kanamycin resistance determinant;Geneious name: npt 
                     CDS"
                     /db_xref="11093881"
                     /protein_id="AAG29472.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     7944..7965
                     /label=pFru97xpMRE100_nptIIGib_fw
     primer_bind     7945..7965
                     /label=pFru97xpMRE101/102nptII_r
     primer_bind     complement(8129..8147)
                     /label=pFru97xpMRE100_nptIIGib_rv
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctg gggccgcaat tcccaattcc
     3241 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3301 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3361 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat
     3421 tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3481 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     3541 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt
     3601 aattccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     3661 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     3721 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccagga
     3781 attaattcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3841 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3901 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3961 aattggggat cggattcgag ctttagtacc tagatttaga tgtctaaaaa gcggggtacc
     4021 gaggacgcgt caattctcga attgacataa gcctgttcgg ttcgtaaact gtaatgcaag
     4081 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     4141 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca
     4201 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     4261 gttacgcagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaggtgg
     4321 ctcaagtatg ggcatcattc gcacatgtag gctcggccct gaccaagtca aatccatgcg
     4381 ggctgctctt gatcttttcg gtcgtgagtt cggagacgta gccacctact cccaacatca
     4441 gccggactcc gattacctcg ggaacttgct ccgtagtaag acattcatcg cgcttgctgc
     4501 cttcgaccaa gaagcggttg ttggcgctct cgcggcttac gttctgccca agtttgagca
     4561 gccgcgtagt gagatctata tctatgatct cgcagtctcc ggcgagcacc ggaggcaggg
     4621 cattgccacc gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg gtgcttatgt
     4681 gatctacgtg caagcagatt acggtgacga tcccgcagtg gctctctata caaagttggg
     4741 catacgggaa gaagtgatgc actttgatat cgacccaagt accgccacct aaagctcgca
     4801 cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc
     4861 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
     4921 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
     4981 gactgggcgg tcttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
     5041 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
     5101 cgcaggggat caagatctga tcaagagaca ggatactagt ggaggaagaa aaaatggtga
     5161 gcaagggcga ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca
     5221 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     5281 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt
     5341 cccctcagtt catgtacggc tccagggcct tcatcaagca ccccgccgac atccccgact
     5401 actataagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     5461 gcggcgccgt gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg
     5521 tgaagctccg cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg
     5581 gctgggaagc gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta
     5641 agatggccct gcgcctgaag gacggcggcc gctacctggc ggacttcaag accacctaca
     5701 aggccaagaa gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca
     5761 cctcccacaa cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact
     5821 ccaccggcgg catggacgag ctgtacaagt aattagctga gcttggactc ctgttgatag
     5881 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     5941 cgttttttat tggtgagaat ccaagctagc ttggcgagat tttcaggagc taaggaagct
     6001 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     6061 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     6121 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     6181 attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac
     6241 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     6301 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     6361 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     6421 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     6481 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     6541 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc
     6601 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     6661 taattttttt aaggcagtta ttggtgccct taaacgcctg gggtaatgac tctctagctt
     6721 gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     6781 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ctaggagctt gcggccgcca
     6841 gtgtgatgga tatctgcata aaaactgttg taattcatta agcattctgc cgacatggaa
     6901 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     6961 cgtataatat ttgcccatgg gggtgggcga agaactccag catgagatcc ccgcgctgga
     7021 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     7081 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc
     7141 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     7201 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     7261 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     7321 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     7381 gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag
     7441 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     7501 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     7561 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     7621 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     7681 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     7741 ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt
     7801 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     7861 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     7921 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga
     7981 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
     8041 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca
     8101 taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt
     8161 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc caggtggcac
     8221 ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct gggcctgttt ctggcgctgg
     8281 acttcccgct gttccgtcag cagcttttcg cccacggcct tgatgatcgc ggcggccttg
     8341 gcctgcatat cccgattcaa cggccccagg gcgtccagaa cgggcttcag gcgctcccga
     8401 aggt
//
