LOCUS       Exported                8430 bp ds-DNA     circular SYN 23-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMRE147_mClover3_GmR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   EJPARKER
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2018 from SnapGene 4.0.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..799)
                     /codon_start=1
                     /transl_table=11
                     /product="Mob"
                     /label=Mob
                     /note="required for plasmid mobilization;Geneious name: Mob
                     CDS"
                     /db_xref="GI:11093882"
                     /protein_id="AAG29473.1"
                     /translation="WRHTRSCDARSWRKWATWRPVSSTPTASARRPTLTPAGRQRTSTG
                     RPAAPMKRWADCASCCQRSGARTLCWRSSTS*RPARNGGSRPAKNSRRRSSRRRTSGWR
                     TSTGRIAS*RPASTVTKPART*PRSWCR*RRTAGCRPRSSSATKRR*PATRPRLRPLWP
                     I*GCNGASRAARHVTRAFRRSTRPWSGHQWATSPSARKRSSHAPMHRRDWPKSWESQSA
                     LRRRKPWPTG*QKRFGRGMSLPYRPPQERVRCARRPIKPKRRPE"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2455
                     /codon_start=1
                     /transl_table=11
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /note="required for plasmid replication;Geneious name: rep 
                     CDS"
                     /db_xref="GI:11093880"
                     /protein_id="AAG29471.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     2437..2446
                     /label=FWD_primer_PnptII
     primer_bind     2443..2462
                     /label=pMRE130_cassette1_FWD
     primer_bind     2443..2462
                     /label=pGRG36_MRE13X_cassette_FW
     primer_bind     complement(2566..2586)
                     /label=Rev_Tet
     primer_bind     complement(2567..2585)
                     /label=pMRE11-6/7/8/9rev
     primer_bind     2630..2649
                     /label=pMRE130_cassette3_FWD
     primer_bind     2630..2649
                     /label=cassette3_pGRG36overhang_FWD
     primer_bind     2915..2930
                     /label=Chloramp_rev
     primer_bind     3020..3039
                     /label=pMRE130_cassette4_FWD
     primer_bind     3020..3039
                     /label=cassette4_pGRG36overhang_FWD
     primer_bind     3142..3160
                     /label=U-19mer Primer
                     /note="19"
     primer_bind     3142..3158
                     /label=M13F-pUC(-40)
                     /note="17"
     primer_bind     3146..3169
                     /label=pMRE130_cassette2_FWD
     primer_bind     3146..3169
                     /label=pMRE130_fwd_new
     primer_bind     3148..3163
                     /label=tn5_insert_FWD
     primer_bind     3161..3178
                     /label=M13-FP
                     /note="18"
     primer_bind     3162..3182
                     /label=pMRE130inserts_fwd
     primer_bind     3162..3178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3162..3178
                     /label=M13Fa
     primer_bind     3162..3178
                     /label=M13F
                     /note="17"
     primer_bind     3188..3207
                     /label=T7promoter
                     /note="20"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3189..3205
                     /label=T7
                     /note="17"
     misc_feature    3239..3240
                     /label=T1 rrnB terminator region
                     /note="T1 rrnB terminator region;Geneious name: T1 rrnB 
                     terminator region regulatory"
     terminator      3249..3335
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3432..3518
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3615..3701
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3796..3882
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3942..3967
                     /label=cassette5_pGRG36overhang_FWD
     primer_bind     3946..3971
                     /label=cassette_pAG408overhang_FWD
     primer_bind     3948..3973
                     /label=Geneious name: MCS primer
                     /note="Geneious name: MCS primer"
     primer_bind     3948..3973
                     /label=pFru97 MCS fw
     primer_bind     3948..3968
                     /label=REV_pFru_ins
     primer_bind     3966..4002
                     /label=FWD_Gent
     CDS             complement(3974)
                     /codon_start=1
                     /label='cat
                     /translation=""
     CDS             3982..3986
                     /codon_start=1
                     /label=Kanamycin resistance
                     /note="Length: 795;Found at strand: positive;Start codon: 
                     ATG"
                     /translation=""
     promoter        4042..4070
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4241..4792
                     /codon_start=1
                     /label=Gentamycin R
                     /label=Gentamycin resistance
                     /note="Length: 552;Found at strand: positive;Start codon: 
                     TGT"
                     /translation="CYGAATMLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKS
                     MRAALDLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPK
                     FEQPRSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALY
                     TKLGIREEVMHFDIDPSTAT"
     primer_bind     4769..4786
                     /label=pAG408_rainbowFOR
     primer_bind     complement(4773..4805)
                     /label=REV_Gent
     primer_bind     4793..4808
                     /label=FWD_primer_PnptII
     promoter        4799..5137
                     /label=p-nptII promoter
                     /label=p-nptII
     primer_bind     4799..4818
                     /label=11x-nptII-FL-fw
     primer_bind     4799..4813
                     /label=lederm_nptII.fw
     primer_bind     complement(4950..4974)
                     /label=pntpII_1_REV
     primer_bind     4950..4974
                     /label=pntpII_1_FWD
     primer_bind     4951..4969
                     /label=pFru97xpMRE100_nptIIGib_rv
     primer_bind     complement(5136..5168)
                     /label=REV_primer_PnptII_mTq2/sG2
     primer_bind     complement(5136..5165)
                     /label=REV_primer_PnptII_mCl/mO/mSc/sY2
     primer_bind     complement(5136..5161)
                     /label=REV_primer_PnptII_mTagBFP2
     primer_bind     5146..5178
                     /label=FWD_primer_mTq2/sGFP2
     primer_bind     5146..5175
                     /label=FWD_primer_mCl/mO/mSc/sY2
     primer_bind     5154..5171
                     /label=XhoI_Ptac_XFP_for
     CDS             5156..5872
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     5156..5175
                     /label=FWD_sYFP2
     primer_bind     5156..5173
                     /label=XFPs for
     primer_bind     complement(5156..5173)
                     /label=Ptuf rev
     primer_bind     complement(5201..5222)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5677..5697)
                     /label=FWD_primer_PnptII
     primer_bind     5809..5830
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5823..5842)
                     /label=REV_primer_sG2
     primer_bind     complement(5853..5895)
                     /label=REV_primer_mTq2
     primer_bind     complement(5854..5877)
                     /label=NotI_XFP_rev
     primer_bind     complement(5854..5875)
                     /label=nextgenFP.rv
     primer_bind     complement(5856..5895)
                     /label=REV_primer_mO
     primer_bind     complement(5856..5875)
                     /label=11x-FL-rv
     primer_bind     complement(5857..5895)
                     /label=REV_primer_sY2
     primer_bind     complement(5857..5875)
                     /label=REV_sYFP2
     primer_bind     complement(5857..5875)
                     /label=Ptac+eXFP_rev
     primer_bind     complement(5858..5875)
                     /label=XFPs rev KpnI
     primer_bind     5876..5890
                     /label=Gib_Gent.rev
     primer_bind     complement(5883..5902)
                     /label=FWD_pFru_ins
     primer_bind     5886..5908
                     /label=Gib_Gent.for.cor
     terminator      5892..5986
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(5909..5927)
                     /label=pQE-R
                     /note="19"
     CDS             6030..6689
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6091..6119)
                     /label=pMRE130inserts_rev
     primer_bind     complement(6674..6689)
                     /label=Chloramp_rev
     terminator      6754..6840
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(6845..6862)
                     /label=Gib_Gent.for
     primer_bind     complement(6850..6867)
                     /label=pMRE130_cassette2_RV
     primer_bind     complement(6851..6867)
                     /label=cassette_pAG408overhang_RV
     primer_bind     complement(6862..6884)
                     /label=tn5_insert_REV
     primer_bind     complement(7063..7082)
                     /label=pMRE130_cassette3_RV
     primer_bind     complement(7063..7082)
                     /label=cassette3_pGRG36overhang_RV
     primer_bind     complement(7152..7172)
                     /label=cassette4_pGRG36overhang_RV
     primer_bind     complement(7152..7171)
                     /label=pMRE130_cassette4_RV
     primer_bind     complement(7174..7193)
                     /label=pMRE130_cassette1_RV
     primer_bind     complement(7174..7193)
                     /label=pGRG36_MRE13X_cassette_RV
     CDS             complement(7179..7973)
                     /codon_start=1
                     /transl_table=11
                     /gene="npt"
                     /product="kanamycin phosphotransferase"
                     /label=npt
                     /note="kanamycin resistance determinant;Geneious name: npt 
                     CDS"
                     /db_xref="GI:11093881"
                     /protein_id="AAG29472.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     7970..7991
                     /label=pFru97xpMRE100_nptIIGib_fw
     primer_bind     7971..7991
                     /label=pFru97xpMRE101/102nptII_r
     primer_bind     8150..8174
                     /label=pntpII_1_REV
     primer_bind     complement(8150..8174)
                     /label=pntpII_1_FWD
     primer_bind     complement(8155..8173)
                     /label=pFru97xpMRE100_nptIIGib_rv
     CDS             complement(8231..8430)
                     /codon_start=1
                     /transl_table=11
                     /product="Mob"
                     /label=Mob
                     /note="required for plasmid mobilization;Geneious name: Mob
                     CDS"
                     /db_xref="GI:11093882"
                     /protein_id="AAG29473.1"
                     /translation="MFGSA*SPFWTPWGR*IGICRPRPPRSSRPWAKSC*RNSGKSSAR
                     NRPSASRNAGAHISPKSATWD"
     primer_bind     complement(8237..8254)
                     /label=pQE-F
                     /note="18"
     primer_bind     complement(8237..8253)
                     /label=QE Promoter
                     /note="17"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctg gggccgcaat tcccaattcc
     3241 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3301 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3361 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat
     3421 tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3481 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     3541 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt
     3601 aattccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     3661 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     3721 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccagga
     3781 attaattcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3841 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3901 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3961 aattggggat cggattcgag ctttagtacc tagatttaga tgtctaaaaa gcggggtacc
     4021 gaggacgcgt caattctcga attgacataa gcctgttcgg ttcgtaaact gtaatgcaag
     4081 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     4141 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca
     4201 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     4261 gttacgcagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaggtgg
     4321 ctcaagtatg ggcatcattc gcacatgtag gctcggccct gaccaagtca aatccatgcg
     4381 ggctgctctt gatcttttcg gtcgtgagtt cggagacgta gccacctact cccaacatca
     4441 gccggactcc gattacctcg ggaacttgct ccgtagtaag acattcatcg cgcttgctgc
     4501 cttcgaccaa gaagcggttg ttggcgctct cgcggcttac gttctgccca agtttgagca
     4561 gccgcgtagt gagatctata tctatgatct cgcagtctcc ggcgagcacc ggaggcaggg
     4621 cattgccacc gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg gtgcttatgt
     4681 gatctacgtg caagcagatt acggtgacga tcccgcagtg gctctctata caaagttggg
     4741 catacgggaa gaagtgatgc actttgatat cgacccaagt accgccacct aaagctcgca
     4801 cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc
     4861 agtccgcaga aacggtgctg accccgggat gaatgtcagc tactggggct atctggacaa
     4921 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
     4981 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
     5041 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     5101 ggcgcagggg atcaagatct gatcaagaga caggatacta gtggaggaag aaaaaatggt
     5161 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     5221 cgtaaacggc cacaagttca gcgtccgcgg cgagggcgag ggcgatgcca ccaacggcaa
     5281 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     5341 gaccaccttc ggctacggcg tggcctgctt cagccgctac cccgaccaca tgaagcagca
     5401 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tctctttcaa
     5461 ggacgacggt acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5521 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5581 ggagtacaac ttcaacagcc actacgtcta tatcacggcc gacaagcaga agaactgcat
     5641 caaggctaac ttcaagatcc gccacaacgt tgaggacggc agcgtgcagc tcgccgacca
     5701 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5761 gagccatcag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5821 ggagttcgtg accgccgccg ggattacaca tggcatggac gagctgtaca agtaataatt
     5881 agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
     5941 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gctagcttgg
     6001 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     6061 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     6121 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     6181 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     6241 cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     6301 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     6361 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     6421 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     6481 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     6541 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     6601 ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     6661 actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
     6721 cgcctggggt aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa
     6781 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     6841 ccgccgctag gagcttgcgg ccgccagtgt gatggatatc tgcataaaaa ctgttgtaat
     6901 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     6961 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatgggggt gggcgaagaa
     7021 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     7081 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     7141 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     7201 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     7261 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     7321 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     7381 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     7441 atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     7501 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     7561 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     7621 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     7681 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     7741 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca
     7801 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     7861 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     7921 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     7981 gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc
     8041 aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct
     8101 ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc
     8161 ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag
     8221 tagctgacat tcatcccagg tggcactttt cggggaaatg tgcgcgcccg cgttcctgct
     8281 ggcgctgggc ctgtttctgg cgctggactt cccgctgttc cgtcagcagc ttttcgccca
     8341 cggccttgat gatcgcggcg gccttggcct gcatatcccg attcaacggc cccagggcgt
     8401 ccagaacggg cttcaggcgc tcccgaaggt
//
