LOCUS       Exported                8469 bp ds-DNA     circular SYN 23-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   EJPARKER
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2018 from SnapGene 4.0.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1023..1792
                     /locus_tag=""
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid p
                     BBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep
                     protein for replication"
     CDS             1793..2455
                     /codon_start=1
                     /locus_tag=""
                     /product="Rep"
                     /label=rep
                     /note="required for plasmid replication;Geneious name: rep 
                     CDS"
                     /db_xref="11093880"
                     /protein_id="AAG29471.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     2437..2446
                     /label=FWD_primer_PnptII
     primer_bind     2443..2462
                     /label=pMRE130_cassette1_FWD
     primer_bind     2443..2462
                     /label=pGRG36_MRE13X_cassette_FW
     primer_bind     complement(2566..2586)
                     /label=Rev_Tet
     primer_bind     complement(2567..2585)
                     /label=pMRE11-6/7/8/9rev
     primer_bind     2630..2649
                     /label=pMRE130_cassette3_FWD
     primer_bind     2630..2649
                     /label=cassette3_pGRG36overhang_FWD
     primer_bind     2915..2930
                     /label=Chloramp_rev
     primer_bind     3020..3039
                     /label=pMRE130_cassette4_FWD
     primer_bind     3020..3039
                     /label=cassette4_pGRG36overhang_FWD
     primer_bind     3146..3169
                     /label=pMRE130_cassette2_FWD
     primer_bind     3146..3169
                     /label=pMRE130_fwd_new
     primer_bind     3148..3163
                     /label=tn5_insert_FWD
     primer_bind     3162..3182
                     /label=pMRE130inserts_fwd
     primer_bind     3162..3178
                     /locus_tag=""
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar va
                     riants"
     primer_bind     3162..3178
                     /label=M13F
     promoter        3188..3206
                     /locus_tag=""
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3239..3240
                     /locus_tag=""
                     /product=""
                     /label=T1 rrnB terminator region
                     /note="T1 rrnB terminator region;Geneious name: T1 rrnB ter
                     minator region regulatory"
     terminator      3249..3335
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3432..3518
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3615..3701
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3796..3882
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     primer_bind     3942..3967
                     /label=cassette5_pGRG36overhang_FWD
     primer_bind     3946..3971
                     /label=cassette_pAG408overhang_FWD
     primer_bind     3948..3973
                     /locus_tag=""
                     /label=Geneious name: MCS primer
                     /note="Geneious name: MCS primer"
     primer_bind     3948..3973
                     /label=pFru97 MCS fw
     primer_bind     3948..3968
                     /label=REV_pFru_ins
     primer_bind     3966..4001
                     /label=73_FWD_Kan
     primer_bind     3985..4021
                     /label=pMRE12-6/7/8/9for
     primer_bind     3985..4021
                     /label=pMRE123for
     CDS             4038..4832
                     /codon_start=1
                     /label=Kanamycin resistance
                     /note="Length: 795;Found at strand: positive;Start codon: 
                     ATG"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     GQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDVGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(4786..4832)
                     /label=pMRE12-6/7/8/9rev
     primer_bind     complement(4813..4845)
                     /label=74_RV_Kan
     primer_bind     4832..4848
                     /label=FWD_primer_PnptII
     promoter        4839..5175
                     /locus_tag=""
                     /label=p-nptII promoter
                     /label=p-nptII
     primer_bind     4839..4858
                     /label=11x-nptII-FL-fw
     primer_bind     4839..4853
                     /label=lederm_nptII.fw
     primer_bind     complement(4988..5012)
                     /label=pntpII_1_REV
     primer_bind     4988..5012
                     /label=pntpII_1_FWD
     primer_bind     4989..5007
                     /label=pFru97xpMRE100_nptIIGib_rv
     primer_bind     complement(5110..5130)
                     /label=pntpII_2_REV
     primer_bind     5110..5130
                     /label=pntpII_2_FWD
     primer_bind     complement(5154..5188)
                     /label=rbs_1_REV
     primer_bind     5154..5188
                     /label=rbs_1_FWD
     primer_bind     complement(5174..5206)
                     /label=REV_primer_PnptII_mTq2/sG2
     primer_bind     complement(5174..5203)
                     /label=REV_primer_PnptII_mCl/mO/mSc/sY2
     primer_bind     complement(5174..5199)
                     /label=REV_primer_PnptII_mTagBFP2
     primer_bind     5184..5216
                     /label=FWD_primer_mTq2/sGFP2
     primer_bind     5184..5213
                     /label=FWD_primer_mCl/mO/mSc/sY2
     primer_bind     5192..5209
                     /label=XhoI_Ptac_XFP_for
     CDS             5194..5914
                     /codon_start=1
                     /locus_tag=""
                     /product=""
                     /label=sYFP2
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     5194..5213
                     /label=FWD_sYFP2
     primer_bind     5194..5211
                     /label=XFPs for
     primer_bind     complement(5194..5211)
                     /label=Ptuf rev
     primer_bind     complement(5715..5735)
                     /label=FWD_primer_PnptII
     primer_bind     complement(5861..5880)
                     /label=REV_primer_sG2
     primer_bind     complement(5891..5934)
                     /label=REV_primer_mTq2
     primer_bind     complement(5892..5914)
                     /label=nextgenFP.rv
     primer_bind     complement(5892..5913)
                     /label=NotI_XFP_rev
     primer_bind     complement(5894..5934)
                     /label=REV_primer_mO
     primer_bind     complement(5894..5913)
                     /label=11x-FL-rv
     primer_bind     complement(5895..5934)
                     /label=REV_primer_sY2
     primer_bind     complement(5895..5914)
                     /label=REV_sYFP2
     primer_bind     complement(5895..5914)
                     /label=Ptac+eXFP_rev
     primer_bind     complement(5896..5914)
                     /label=XFPs rev KpnI
     primer_bind     complement(5922..5941)
                     /label=FWD_pFru_ins
     primer_bind     5925..5947
                     /label=Gib_Gent.for.cor
     terminator      5931..6025
                     /locus_tag=""
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             6069..6728
                     /codon_start=1
                     /locus_tag=""
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6130..6158)
                     /label=pMRE130inserts_rev
     primer_bind     complement(6713..6728)
                     /label=Chloramp_rev
     terminator      6793..6879
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     primer_bind     complement(6884..6901)
                     /label=Gib_Gent.for
     primer_bind     complement(6889..6906)
                     /label=pMRE130_cassette2_RV
     primer_bind     complement(6890..6906)
                     /label=cassette_pAG408overhang_RV
     primer_bind     complement(6901..6923)
                     /label=tn5_insert_REV
     primer_bind     complement(7102..7121)
                     /label=pMRE130_cassette3_RV
     primer_bind     complement(7102..7121)
                     /label=cassette3_pGRG36overhang_RV
     primer_bind     complement(7191..7211)
                     /label=cassette4_pGRG36overhang_RV
     primer_bind     complement(7191..7210)
                     /label=pMRE130_cassette4_RV
     primer_bind     complement(7213..7232)
                     /label=pMRE130_cassette1_RV
     primer_bind     complement(7213..7232)
                     /label=pGRG36_MRE13X_cassette_RV
     CDS             complement(7218..8012)
                     /codon_start=1
                     /locus_tag=""
                     /product="kanamycin phosphotransferase"
                     /label=npt
                     /note="kanamycin resistance determinant;Geneious name: npt 
                     CDS"
                     /db_xref="11093881"
                     /protein_id="AAG29472.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     8009..8030
                     /label=pFru97xpMRE100_nptIIGib_fw
     primer_bind     8010..8030
                     /label=pFru97xpMRE101/102nptII_r
     primer_bind     8027..8047
                     /label=rbs_1_REV
     primer_bind     8071..8091
                     /label=pntpII_2_REV
     primer_bind     complement(8071..8091)
                     /label=pntpII_2_FWD
     primer_bind     8189..8213
                     /label=pntpII_1_REV
     primer_bind     complement(8189..8213)
                     /label=pntpII_1_FWD
     primer_bind     complement(8194..8212)
                     /label=pFru97xpMRE100_nptIIGib_rv
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctg gggccgcaat tcccaattcc
     3241 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3301 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3361 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat
     3421 tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3481 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     3541 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt
     3601 aattccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     3661 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     3721 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccagga
     3781 attaattcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3841 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3901 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3961 aattggggat cggattcgag ctaagaattc ccttggggta tctttaaata ctgtagaaaa
     4021 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
     4081 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
     4141 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
     4201 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
     4261 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
     4321 gcgtcctttg ctcggaagag tatgaagatg gacaaagccc tgaaaagatt atcgagctgt
     4381 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
     4441 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
     4501 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt
     4561 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
     4621 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
     4681 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat gtcggggaag
     4741 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
     4801 aatattatat tttactggat gaattgtttt agagctcgca cgctgccgca agcactcagg
     4861 gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg
     4921 accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag
     4981 aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac
     5041 agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa
     5101 agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga
     5161 tcaagagaca ggatactagt ggaggaagaa aaaatggtga gcaagggcga ggagctgttc
     5221 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     5281 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     5341 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcgtg
     5401 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     5461 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     5521 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5581 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5641 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     5701 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     5761 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
     5821 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5881 atcactctcg gcatggacga gctgtacaag taagtaatta gctgagcttg gactcctgtt
     5941 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
     6001 ccgggcgttt tttattggtg agaatccaag ctagcttggc gagattttca ggagctaagg
     6061 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     6121 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     6181 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     6241 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
     6301 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     6361 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     6421 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     6481 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     6541 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     6601 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg
     6661 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     6721 gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggggta atgactctct
     6781 agcttgaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     6841 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgctagg agcttgcggc
     6901 cgccagtgtg atggatatct gcataaaaac tgttgtaatt cattaagcat tctgccgaca
     6961 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     7021 ccttgcgtat aatatttgcc catgggggtg ggcgaagaac tccagcatga gatccccgcg
     7081 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     7141 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     7201 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     7261 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     7321 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     7381 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     7441 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
     7501 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     7561 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     7621 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     7681 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     7741 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     7801 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
     7861 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     7921 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     7981 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     8041 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     8101 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     8161 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     8221 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catcccaggt
     8281 ggcacttttc ggggaaatgt gcgcgcccgc gttcctgctg gcgctgggcc tgtttctggc
     8341 gctggacttc ccgctgttcc gtcagcagct tttcgcccac ggccttgatg atcgcggcgg
     8401 ccttggcctg catatcccga ttcaacggcc ccagggcgtc cagaacgggc ttcaggcgct
     8461 cccgaaggt
//
