LOCUS       Exported                9591 bp ds-DNA     circular SYN 23-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   EJPARKER
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2018 from SnapGene 4.0.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1023..1792
                     /locus_tag=""
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid p
                     BBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep
                     protein for replication"
     CDS             1793..2455
                     /codon_start=1
                     /locus_tag=""
                     /product="Rep"
                     /label=rep
                     /note="required for plasmid replication;Geneious name: rep 
                     CDS"
                     /db_xref="11093880"
                     /protein_id="AAG29471.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     2437..2446
                     /label=FWD_primer_PnptII
     primer_bind     2443..2462
                     /label=pMRE130_cassette1_FWD
     primer_bind     2443..2462
                     /label=pGRG36_MRE13X_cassette_FW
     primer_bind     complement(2566..2586)
                     /label=Rev_Tet
     primer_bind     complement(2567..2585)
                     /label=pMRE11-6/7/8/9rev
     primer_bind     2630..2649
                     /label=pMRE130_cassette3_FWD
     primer_bind     2630..2649
                     /label=cassette3_pGRG36overhang_FWD
     primer_bind     2915..2930
                     /label=Chloramp_rev
     primer_bind     3020..3039
                     /label=pMRE130_cassette4_FWD
     primer_bind     3020..3039
                     /label=cassette4_pGRG36overhang_FWD
     primer_bind     3146..3169
                     /label=pMRE130_cassette2_FWD
     primer_bind     3146..3169
                     /label=pMRE130_fwd_new
     primer_bind     3148..3163
                     /label=tn5_insert_FWD
     primer_bind     3162..3182
                     /label=pMRE130inserts_fwd
     primer_bind     3162..3178
                     /locus_tag=""
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar va
                     riants"
     primer_bind     3162..3178
                     /label=M13F
     promoter        3188..3206
                     /locus_tag=""
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3239..3240
                     /locus_tag=""
                     /product=""
                     /label=T1 rrnB terminator region
                     /note="T1 rrnB terminator region;Geneious name: T1 rrnB ter
                     minator region regulatory"
     terminator      3249..3335
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3432..3518
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3615..3701
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     terminator      3796..3882
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     primer_bind     3942..3967
                     /label=cassette5_pGRG36overhang_FWD
     primer_bind     3946..3971
                     /label=cassette_pAG408overhang_FWD
     primer_bind     3948..3973
                     /locus_tag=""
                     /label=Geneious name: MCS primer
                     /note="Geneious name: MCS primer"
     primer_bind     3948..3973
                     /label=pFru97 MCS fw
     primer_bind     3948..3968
                     /label=REV_pFru_ins
     primer_bind     3965..4003
                     /label=91_pMRE130_pTE_tetAtetR_FWD
     CDS             complement(3984..5183)
                     /codon_start=1
                     /transl_table=11
                     /gene="tetA"
                     /product="TetA"
                     /function="tetracycline resistance"
                     /label=tetA
                     /note="SECDescr=TetA
                     SECDrawAs=Gene
                     SECName=tetA
                     SECStyleId=1"
                     /db_xref="GI:14794603"
                     /protein_id="AAK73407.1"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     primer_bind     4127..4171
                     /label=pMRE130inserts_fwd
     CDS             5289..5954
                     /codon_start=1
                     /transl_table=11
                     /gene="tetR"
                     /product="TetR"
                     /function="tetracycline resistance regulation"
                     /label=tetR
                     /note="SECDescr=TetR
                     SECDrawAs=Gene
                     SECName=tetR
                     SECStyleId=1"
                     /db_xref="GI:14794602"
                     /protein_id="AAK73406.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGAKKPLLS"
     primer_bind     complement(5933..5974)
                     /label=92_pMRE130_pTE_tetAtetR_RV
     primer_bind     5955..5970
                     /label=FWD_primer_PnptII
     promoter        5961..6297
                     /locus_tag=""
                     /label=p-nptII promoter
                     /label=p-nptII
     primer_bind     5961..5980
                     /label=11x-nptII-FL-fw
     primer_bind     5961..5975
                     /label=lederm_nptII.fw
     primer_bind     6111..6129
                     /label=pFru97xpMRE100_nptIIGib_rv
     primer_bind     complement(6296..6328)
                     /label=REV_primer_PnptII_mTq2/sG2
     primer_bind     complement(6296..6325)
                     /label=REV_primer_PnptII_mCl/mO/mSc/sY2
     primer_bind     complement(6296..6321)
                     /label=REV_primer_PnptII_mTagBFP2
     primer_bind     6306..6338
                     /label=FWD_primer_mTq2/sGFP2
     primer_bind     6306..6335
                     /label=FWD_primer_mCl/mO/mSc/sY2
     primer_bind     6314..6331
                     /label=XhoI_Ptac_XFP_for
     CDS             6316..7036
                     /codon_start=1
                     /locus_tag=""
                     /product=""
                     /label=sYFP2
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     6316..6335
                     /label=FWD_sYFP2
     primer_bind     6316..6333
                     /label=XFPs for
     primer_bind     complement(6316..6333)
                     /label=Ptuf rev
     primer_bind     complement(6837..6857)
                     /label=FWD_primer_PnptII
     primer_bind     complement(6983..7002)
                     /label=REV_primer_sG2
     primer_bind     complement(7013..7056)
                     /label=REV_primer_mTq2
     primer_bind     complement(7014..7036)
                     /label=nextgenFP.rv
     primer_bind     complement(7014..7035)
                     /label=NotI_XFP_rev
     primer_bind     complement(7016..7056)
                     /label=REV_primer_mO
     primer_bind     complement(7016..7035)
                     /label=11x-FL-rv
     primer_bind     complement(7017..7056)
                     /label=REV_primer_sY2
     primer_bind     complement(7017..7036)
                     /label=REV_sYFP2
     primer_bind     complement(7017..7036)
                     /label=Ptac+eXFP_rev
     primer_bind     complement(7018..7036)
                     /label=XFPs rev KpnI
     primer_bind     complement(7044..7063)
                     /label=FWD_pFru_ins
     primer_bind     7047..7069
                     /label=Gib_Gent.for.cor
     terminator      7053..7147
                     /locus_tag=""
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             7191..7850
                     /codon_start=1
                     /locus_tag=""
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7252..7280)
                     /label=pMRE130inserts_rev
     primer_bind     complement(7835..7850)
                     /label=Chloramp_rev
     terminator      7915..8001
                     /locus_tag=""
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB ge
                     ne"
     primer_bind     complement(8006..8023)
                     /label=Gib_Gent.for
     primer_bind     complement(8011..8028)
                     /label=pMRE130_cassette2_RV
     primer_bind     complement(8012..8028)
                     /label=cassette_pAG408overhang_RV
     primer_bind     complement(8023..8045)
                     /label=tn5_insert_REV
     primer_bind     complement(8224..8243)
                     /label=pMRE130_cassette3_RV
     primer_bind     complement(8224..8243)
                     /label=cassette3_pGRG36overhang_RV
     primer_bind     complement(8313..8333)
                     /label=cassette4_pGRG36overhang_RV
     primer_bind     complement(8313..8332)
                     /label=pMRE130_cassette4_RV
     primer_bind     complement(8335..8354)
                     /label=pMRE130_cassette1_RV
     primer_bind     complement(8335..8354)
                     /label=pGRG36_MRE13X_cassette_RV
     CDS             complement(8340..9134)
                     /codon_start=1
                     /locus_tag=""
                     /product="kanamycin phosphotransferase"
                     /label=npt
                     /note="kanamycin resistance determinant;Geneious name: npt 
                     CDS"
                     /db_xref="11093881"
                     /protein_id="AAG29472.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     9131..9152
                     /label=pFru97xpMRE100_nptIIGib_fw
     primer_bind     9132..9152
                     /label=pFru97xpMRE101/102nptII_r
     primer_bind     complement(9316..9334)
                     /label=pFru97xpMRE100_nptIIGib_rv
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctg gggccgcaat tcccaattcc
     3241 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3301 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3361 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat
     3421 tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3481 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     3541 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt
     3601 aattccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     3661 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     3721 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccagga
     3781 attaattcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3841 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3901 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3961 aattggggat cggattcgag ctctcagcga tcggctcgtt gccctgcgcc gctccaaagc
     4021 ccgcgacgca gcgccggcag gcagagcaag tagagggcag cgcctgcaat ccatgcccac
     4081 ccgttccacg ttgttataga agccgcatag atcgccgtga agaggagggg tccgacgatc
     4141 gaggtcaggc tggtgagcgc cgccagtgag ccttgcagct gcccctgacg ttcctcatcc
     4201 acctgcctgg acaacattgc ttgcagcgcc ggcattccga tgccacccga agcaagcagg
     4261 accatgatcg ggaacgccat ccatccccgt gtcgcgaagg caagcaggat gtagcctgtg
     4321 ccgtcggcaa tcattccgag catgagtgcc cgcctttcgc cgagccgggc ggctacaggg
     4381 ccggtgatca ttgcctgggc gagtgaatgc agaatgccaa atgcggcaag cgaaatgccg
     4441 atcgtggtcg cgtcccagtg aaagcgatcc tcgccgaaaa tgacccaaag cgcggccggc
     4501 acctgtccga caagttgcat gatgaagaag accgccatca gggcggcgac gacggtcatg
     4561 ccccgggccc accggaacga agcgagcggg ttgagagcct cccggcgtaa cggccggcgt
     4621 tcgcctttgt gcgactccgg caaaaggaaa cagcccgtca ggaaattgag gccgttcaag
     4681 gctgccgcgg cgaagaacgg agcgtggggg gagaaaccgc ccatcagccc accgagcaca
     4741 ggtcccgcga ccatcccgaa cccgaaacag gcgctcatga agccgaagtg ccgcgcgcgc
     4801 tcatcgccat cagtgatatc ggcaatataa gcgccggcta ccgccccagt cgccccggtg
     4861 atgccggcca cgatccgccc gatatagaga acccaaagga aaggcgctgt cgccatgatg
     4921 gcgtagtcga cagtggcgcc ggccagcgag acgagcaaga ttggccgccg cccgaaacga
     4981 tccgacagcg cgcccagcac aggtgcgcag gcaaattgca ccaacgcata cagcgccagc
     5041 agaatgccat agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc
     5101 agcaccggca taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct cagaattacg
     5161 atcaggggta tgttgggttt cacgtctggc ctccggacca gcctccgctg gtccgattga
     5221 acgggcggat tctttatcac tgataagttg gtggacatat tatgtttatc agtgataaag
     5281 tgtcaatcat gacaaagttg cagccgaata cagtgatccg tgccgccctg gacctgttga
     5341 acgaggtcgg cgtagacggt ctgacgacac gcaaactggc ggaacggttg ggggttcagc
     5401 agccggcgct ttactggcac ttcaggaaca agcgggcgct gctcgacgca ctggccgaag
     5461 ccatgctggc ggagaatcat acgcattcgg tgccgagagc cgacgacgac tggcgctcat
     5521 ttctgatcgg gaatgcccgc agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc
     5581 gcatccatgc cggcacgcga ccgggcgcac cgcagatgga aacggccgac gcgcagcttc
     5641 gcttcctctg cgaggcgggt ttttcggccg gggacgccgt caatgcgctg atgacaatca
     5701 gctacttcac tgttggggcc gtgcttgagg agcaggccgg cgacagcgat gccggcgagc
     5761 gcggcggcac cgttgaacag gctccgctct cgccgctgtt gcgggccgcg atagacgcct
     5821 tcgacgaagc cggtccggac gcagcgttcg agcagggact cgcggtgatt gtcgatggat
     5881 tggcgaaaag gaggctcgtt gtcaggaacg ttgaaggacc gagaaagggt gctaagaaac
     5941 cattattatc atgaagctcg cacgctgccg caagcactca gggcgcaagg gctgctaaag
     6001 gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
     6061 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
     6121 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
     6181 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
     6241 cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatacta
     6301 gtggaggaag aaaaaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     6361 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     6421 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     6481 gtgccctggc ccaccctcgt gaccaccctg ggctacggcg tgcagtgctt cgcccgctac
     6541 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     6601 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     6661 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     6721 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     6781 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     6841 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     6901 ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc caacgagaag
     6961 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     7021 gagctgtaca agtaagtaat tagctgagct tggactcctg ttgatagatc cagtaatgac
     7081 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
     7141 tgagaatcca agctagcttg gcgagatttt caggagctaa ggaagctaaa atggagaaaa
     7201 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     7261 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     7321 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     7381 cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga
     7441 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     7501 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     7561 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     7621 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     7681 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     7741 tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa
     7801 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
     7861 gcagttattg gtgcccttaa acgcctgggg taatgactct ctagcttgag gcatcaaata
     7921 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     7981 gctctcctga gtaggacaaa tccgccgcta ggagcttgcg gccgccagtg tgatggatat
     8041 ctgcataaaa actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     8101 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     8161 cccatggggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     8221 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     8281 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     8341 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     8401 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     8461 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     8521 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     8581 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     8641 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     8701 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     8761 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     8821 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     8881 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     8941 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     9001 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     9061 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     9121 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg
     9181 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc
     9241 ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag
     9301 tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt
     9361 tcccttgtcc agatagccca gtagctgaca ttcatcccag gtggcacttt tcggggaaat
     9421 gtgcgcgccc gcgttcctgc tggcgctggg cctgtttctg gcgctggact tcccgctgtt
     9481 ccgtcagcag cttttcgccc acggccttga tgatcgcggc ggccttggcc tgcatatccc
     9541 gattcaacgg ccccagggcg tccagaacgg gcttcaggcg ctcccgaagg t
//
