LOCUS       19_pmax__CPSF2_3        6674 bp ds-DNA     circular     02-SEP-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Insert from pCR8-hcoRBM38-3xNLS-MCP_3xFLAG-28-51-78 652 to 2141
COMMENT     Backbone from pAC90-pmaxGW-D55-60,22-61-69,23-91-7 2650 to 967
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          4703..4713
                     /organism="pCR8/GW/TOPO"
                     /mol_type="other DNA"
                     /label=source:pCR8/GW/TOPO
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          1..947
                     /organism="pmaxCloning"
                     /mol_type="other DNA"
                     /label=source:pmaxCloning
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1011..1379
                     /label=FKBP
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          1557..4553
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          4847..6674
                     /organism="pmaxCloning"
                     /mol_type="other DNA"
                     /label=source:pmaxCloning(1)
                     /ApEinfo_label=source:pmaxCloning
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1560..1580
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label=SV40 NLS
                     /ApEinfo_fwdcolor=#e9d024
                     /ApEinfo_revcolor=#e9d024
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        163..715
                     /label=CMV_immearly_promoter
                     /ApEinfo_fwdcolor=#ffaae6
                     /ApEinfo_revcolor=#ff389a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4617..4685
                     /label=3xFLAG
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4554..4554
                     /label=HA-tag
                     /ApEinfo_fwdcolor=#ff00ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    218..505
                     /label=CAG_enhancer
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(1407..1478,1485..1496)
                     /label=3xNLS
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4497..4517
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label=SV40 NLS(1)
                     /ApEinfo_label=SV40 NLS
                     /ApEinfo_fwdcolor=#e9d024
                     /ApEinfo_revcolor=#e9d024
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        661..742
                     /label=CMV_promoter
                     /ApEinfo_fwdcolor=#ffaae6
                     /ApEinfo_revcolor=#ff389a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4704..4713
                     /label=attL2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4527..4553
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /label=HA
                     /ApEinfo_fwdcolor=#e9d024
                     /ApEinfo_revcolor=#e9d024
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          960..967
                     /organism="pLenti6/Ubc/V5-DEST Gateway (ViraPower)"
                     /mol_type="other DNA"
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          4714..4834
                     /organism="pLenti6/Ubc/V5-DEST Gateway (ViraPower)"
                     /mol_type="other DNA"
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)(1)
                     /ApEinfo_label=source:pLenti6/Ubc/V5-DEST Gateway
                     (ViraPower)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4554..4554
                     /label=dCasRx
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(5141..5759)
                     /label=pBR322_origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    960..967
                     /label=attR1
                     /ApEinfo_fwdcolor=#ffaa89
                     /ApEinfo_revcolor=#deff79
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    672..692
                     /label=CMV_fwd_primer
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          968..991
                     /organism="pCR8/GW/TOPO"
                     /mol_type="other DNA"
                     /label=source:pCR8/GW/TOPO(1)
                     /ApEinfo_label=source:pCR8/GW/TOPO
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(968..984)
                     /label=attL1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    813..945
                     /label=chim_int
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    987..991
                     /label=EcoRIHS
                     /ApEinfo_fwdcolor=#ff8b8e
                     /ApEinfo_revcolor=#ff8b8e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4781..4800)
                     /label=AC_V5_R
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(5824..6669)
                     /translation="MGGDRDETNPDKCFNNIMIEQDGLHAGSPAAWVERLFGYDWAQQ
                     TIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVT
                     EAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIER
                     ARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENG
                     RFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRL
                     LDEFF*"
                     /label=ORF frame 3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4848..4867)
                     /label=EBV_rev_primer
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(5827..6606)
                     /gene="NeoR/KanR (variant)"
                     /translation="MGGDRDETNPDKCFNNIMIEQDGLHAGSPAAWVERLFGYDWAQQ
                     TIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVT
                     EAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIER
                     ARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENG
                     RFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRL
                     LDEFF*"
                     /label=NeoR/KanR (variant)
                     /ApEinfo_fwdcolor=#0004ff
                     /ApEinfo_revcolor=#ff00a8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4781..4822
                     /label=V5
                     /ApEinfo_fwdcolor=#ffaa89
                     /ApEinfo_revcolor=#deff79
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1557..4553
                     /label=dCasRxHsu
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
      901 cacctattgg tcttactgac atccactttg cctttctctc cacagggaaa aaaCAATTGA
      961 CAAGTTTGTA CAAAAAAGCA GGCTCCGAAT TcACCGGTgc cgccaccATG GGAGGTGGCT
     1021 CATCTGGCGG AGGTCAGATC TCGTACGCGT CCCGGGGCGG AGTGCAGGTG GAAACCATCT
     1081 CCCCAGGAGA CGGGCGCACC TTCCCCAAGC GCGGCCAGAC CTGCGTGGTG CACTACACCG
     1141 GGATGCTTGA AGATGGAAAG AAATTTGATT CCTCCCGGGA CAGAAACAAG CCCTTTAAGT
     1201 TTATGCTAGG CAAGCAGGAG GTGATCCGAG GCTGGGAAGA AGGGGTTGCC CAGATGAGTG
     1261 TGGGTCAGAG AGCCAAACTG ACTATATCTC CAGATTATGC CTATGGTGCC ACTGGGCACC
     1321 CAGGCATCAT CCCACCACAT GCCACTCTCG TCTTCGATGT GGAGCTTCTA AAACTGGAAa
     1381 acgttatcga tGGCGGAGGC GGATCTGATC CAAAAAAGAA GAGAAAGGTA GATCCAAAAA
     1441 AGAAGAGAAA GGTAGATCCA AAAAAGAAGA GAAAGGTAGG ATCTACCGGA TCTAGAaacg
     1501 atGGTGGTGG TGGAAGCGGG GGTGGGGGCA GCGGTGGAGG GGGAAGCggg cgcgccAGCC
     1561 CCAAGAAGAA GAGAAAGGTG GAGGCCAGCA TCGAAAAAAA AAAGTCCTTC GCCAAGGGCA
     1621 TGGGCGTGAA GTCCACACTC GTGTCCGGCT CCAAAGTGTA CATGACAACC TTCGCCGAAG
     1681 GCAGCGACGC CAGGCTGGAA AAGATCGTGG AGGGCGACAG CATCAGGAGC GTGAATGAGG
     1741 GCGAGGCCTT CAGCGCTGAA ATGGCCGATA AAAACGCCGG CTATAAGATC GGCAACGCCA
     1801 AATTCAGCCA TCCTAAGGGC TACGCCGTGG TGGCTAACAA CCCTCTGTAT ACAGGACCCG
     1861 TCCAGCAGGA TATGCTCGGC CTGAAGGAAA CTCTGGAAAA GAGGTACTTC GGCGAGAGCG
     1921 CTGATGGCAA TGACAATATT TGTATCCAGG TGATCCATAA CATCCTGGAC ATTGAAAAAA
     1981 TCCTCGCCGA ATACATTACC AACGCCGCCT ACGCCGTCAA CAATATCTCC GGCCTGGATA
     2041 AGGACATTAT TGGATTCGGC AAGTTCTCCA CAGTGTATAC CTACGACGAA TTCAAAGACC
     2101 CCGAGCACCA TAGGGCCGCT TTCAACAATA ACGATAAGCT CATCAACGCC ATCAAGGCCC
     2161 AGTATGACGA GTTCGACAAC TTCCTCGATA ACCCCAGACT CGGCTATTTC GGCCAGGCCT
     2221 TTTTCAGCAA GGAGGGCAGA AATTACATCA TCAATTACGG CAACGAATGC TATGACATTC
     2281 TGGCCCTCCT GAGCGGACTG GCGCACTGGG TGGTCGCTAA CAACGAAGAA GAGTCCAGGA
     2341 TCTCCAGGAC CTGGCTCTAC AACCTCGATA AGAACCTCGA CAACGAATAC ATCTCCACCC
     2401 TCAACTACCT CTACGACAGG ATCACCAATG AGCTGACCAA CTCCTTCTCC AAGAACTCCG
     2461 CCGCCAACGT GAACTATATT GCCGAAACTC TGGGAATCAA CCCTGCCGAA TTCGCCGAAC
     2521 AATATTTCAG ATTCAGCATT ATGAAAGAGC AGAAAAACCT CGGATTCAAT ATCACCAAGC
     2581 TCAGGGAAGT GATGCTGGAC AGGAAGGATA TGTCCGAGAT CAGGAAAAAT CATAAGGTGT
     2641 TCGACTCCAT CAGGACCAAG GTCTACACCA TGATGGACTT TGTGATTTAT AGGTATTACA
     2701 TCGAAGAGGA TGCCAAGGTG GCTGCCGCCA ATAAGTCCCT CCCCGATAAT GAGAAGTCCC
     2761 TGAGCGAGAA GGATATCTTT GTGATTAACC TGAGGGGCTC CTTCAACGAC GACCAGAAGG
     2821 ATGCCCTCTA CTACGATGAA GCTAATAGAA TTTGGAGAAA GCTCGAAAAT ATCATGCACA
     2881 ACATCAAGGA ATTTAGGGGA AACAAGACAA GAGAGTATAA GAAGAAGGAC GCCCCTAGAC
     2941 TGCCCAGAAT CCTGCCCGCT GGCCGTGATG TTTCCGCCTT CAGCAAACTC ATGTATGCCC
     3001 TGACCATGTT CCTGGATGGC AAGGAGATCA ACGACCTCCT GACCACCCTG ATTAATAAAT
     3061 TCGATAACAT CCAGAGCTTC CTGAAGGTGA TGCCTCTCAT CGGAGTCAAC GCTAAGTTCG
     3121 TGGAGGAATA CGCCTTTTTC AAAGACTCCG CCAAGATCGC CGATGAGCTG AGGCTGATCA
     3181 AGTCCTTCGC TAGAATGGGA GAACCTATTG CCGATGCCAG GAGGGCCATG TATATCGACG
     3241 CCATCCGTAT TTTAGGAACC AACCTGTCCT ATGATGAGCT CAAGGCCCTC GCCGACACCT
     3301 TTTCCCTGGA CGAGAACGGA AACAAGCTCA AGAAAGGCAA GCACGGCATG AGAAATTTCA
     3361 TTATTAATAA CGTGATCAGC AATAAAAGGT TCCACTACCT GATCAGATAC GGTGATCCTG
     3421 CCCACCTCCA TGAGATCGCC AAAAACGAGG CCGTGGTGAA GTTCGTGCTC GGCAGGATCG
     3481 CTGACATCCA GAAAAAACAG GGCCAGAACG GCAAGAACCA GATCGACAGG TACTACGAAA
     3541 CTTGTATCGG AAAGGATAAG GGCAAGAGCG TGAGCGAAAA GGTGGACGCT CTCACAAAGA
     3601 TCATCACCGG AATGAACTAC GACCAATTCG ACAAGAAAAG GAGCGTCATT GAGGACACCG
     3661 GCAGGGAAAA CGCCGAGAGG GAGAAGTTTA AAAAGATCAT CAGCCTGTAC CTCACCGTGA
     3721 TCTACCACAT CCTCAAGAAT ATTGTCAATA TCAACGCCAG GTACGTCATC GGATTCCATT
     3781 GCGTCGAGCG TGATGCTCAA CTGTACAAGG AGAAAGGCTA CGACATCAAT CTCAAGAAAC
     3841 TGGAAGAGAA GGGATTCAGC TCCGTCACCA AGCTCTGCGC TGGCATTGAT GAAACTGCCC
     3901 CCGATAAGAG AAAGGACGTG GAAAAGGAGA TGGCTGAAAG AGCCAAGGAG AGCATTGACA
     3961 GCCTCGAGAG CGCCAACCCC AAGCTGTATG CCAATTACAT CAAATACAGC GACGAGAAGA
     4021 AAGCCGAGGA GTTCACCAGG CAGATTAACA GGGAGAAGGC CAAAACCGCC CTGAACGCCT
     4081 ACCTGAGGAA CACCAAGTGG AATGTGATCA TCAGGGAGGA CCTCCTGAGA ATTGACAACA
     4141 AGACATGTAC CCTGTTCGCA AACAAGGCCG TCGCCCTGGA AGTGGCCAGG TATGTCCACG
     4201 CCTATATCAA CGACATTGCC GAGGTCAATT CCTACTTCCA ACTGTACCAT TACATCATGC
     4261 AGAGAATTAT CATGAATGAG AGGTACGAGA AAAGCAGCGG AAAGGTGTCC GAGTACTTCG
     4321 ACGCTGTGAA TGACGAGAAG AAGTACAACG ATAGGCTCCT GAAACTGCTG TGTGTGCCTT
     4381 TCGGCTACTG TATCCCCAGG TTTAAGAACC TGAGCATCGA GGCCCTGTTC GATAGGAACG
     4441 AGGCCGCCAA GTTCGACAAG GAGAAAAAGA AGGTGTCCGG CAATTCCGGA TCCGGACCTA
     4501 AGAAAAAGAG GAAGGTGGCG GCCGCTTACC CATACGATGT TCCAGATTAC GCTggcggcc
     4561 gcGGGGGAGG CGGTTCCGGT GGCGGCGGAA GCGGAGGTGG AGGATCAgGG CCGGCCATGG
     4621 ACTACAAGGA TCACGACGGT GACTATAAGG ATCATGACAT CGACTATAAG GACGATGACG
     4681 ATAAGtgaTT AATTAAGAAT TCGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCCAGCA
     4741 CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC GCGGTTCGAA GGTAAGCCTA TCCCTAACCC
     4801 TCTCCTCGGT CTCGATTCTA CGCGTACCGG TTAGCAATTG ttttttcgat gagtttggac
     4861 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     4921 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     4981 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     5041 aatgtggtac ttaagagggg gagaccaaag ggcgagacgt taaggcctca cgtgacatgt
     5101 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5161 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5221 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5281 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5341 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5401 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5461 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5521 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5581 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5641 gaaaaagagt tggtagctct tgatccggca aacaaaccac gctggtagcg gtggtttttt
     5701 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5761 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgcc
     5821 gtctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     5881 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     5941 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     6001 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     6061 cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg
     6121 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     6181 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     6241 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     6301 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     6361 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     6421 ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     6481 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     6541 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     6601 tccatcttgt tcaatcataa tattattgaa gcatttatca gggttcgtct cgtcccggtc
     6661 tcctcccatg catg    
//
