LOCUS       pmax__hcoRBM38_3        4331 bp ds-DNA     circular     02-SEP-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Insert from pCR8-hcoRBM38-3xNLS-MCP_3xFLAG-28-51-78 652 to 2141
COMMENT     Backbone from pAC90-pmaxGW-D55-60,22-61-69,23-91-7 2650 to 967
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          2360..2370
                     /organism="pCR8/GW/TOPO"
                     /mol_type="other DNA"
                     /label=source:pCR8/GW/TOPO
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          1..947
                     /organism="pmaxCloning"
                     /mol_type="other DNA"
                     /label=source:pmaxCloning
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1902..2219
                     /label=FRB
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          2504..4331
                     /organism="pmaxCloning"
                     /mol_type="other DNA"
                     /label=source:pmaxCloning(1)
                     /ApEinfo_label=source:pmaxCloning
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2220..2220
                     /label=insertB4
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1008..1724
                     /label=hcoRBM38
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        163..715
                     /label=CMV_immearly_promoter
                     /ApEinfo_fwdcolor=#ffaae6
                     /ApEinfo_revcolor=#ff389a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2274..2342
                     /label=3xFLAG
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    218..505
                     /label=CAG_enhancer
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(1752..1823,1830..1841)
                     /label=3xNLS
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        661..742
                     /label=CMV_promoter
                     /ApEinfo_fwdcolor=#ffaae6
                     /ApEinfo_revcolor=#ff389a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2361..2370
                     /label=attL2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          960..967
                     /organism="pLenti6/Ubc/V5-DEST Gateway (ViraPower)"
                     /mol_type="other DNA"
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          2371..2491
                     /organism="pLenti6/Ubc/V5-DEST Gateway (ViraPower)"
                     /mol_type="other DNA"
                     /label=source:pLenti6/Ubc/V5-DEST Gateway (ViraPower)(1)
                     /ApEinfo_label=source:pLenti6/Ubc/V5-DEST Gateway
                     (ViraPower)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(2798..3416)
                     /label=pBR322_origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    960..967
                     /label=attR1
                     /ApEinfo_fwdcolor=#ffaa89
                     /ApEinfo_revcolor=#deff79
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    672..692
                     /label=CMV_fwd_primer
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          968..991
                     /organism="pCR8/GW/TOPO"
                     /mol_type="other DNA"
                     /label=source:pCR8/GW/TOPO(1)
                     /ApEinfo_label=source:pCR8/GW/TOPO
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(968..984)
                     /label=attL1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    813..945
                     /label=chim_int
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    987..991
                     /label=EcoRIHS
                     /ApEinfo_fwdcolor=#ff8b8e
                     /ApEinfo_revcolor=#ff8b8e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2438..2457)
                     /label=AC_V5_R
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3481..4326)
                     /translation="MGGDRDETNPDKCFNNIMIEQDGLHAGSPAAWVERLFGYDWAQQ
                     TIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVT
                     EAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIER
                     ARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENG
                     RFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRL
                     LDEFF*"
                     /label=ORF frame 3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2505..2524)
                     /label=EBV_rev_primer
                     /ApEinfo_fwdcolor=#33ff00
                     /ApEinfo_revcolor=#bfc4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(3484..4263)
                     /gene="NeoR/KanR (variant)"
                     /translation="MGGDRDETNPDKCFNNIMIEQDGLHAGSPAAWVERLFGYDWAQQ
                     TIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVT
                     EAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIER
                     ARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENG
                     RFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRL
                     LDEFF*"
                     /label=NeoR/KanR (variant)
                     /ApEinfo_fwdcolor=#0004ff
                     /ApEinfo_revcolor=#ff00a8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2438..2479
                     /label=V5
                     /ApEinfo_fwdcolor=#ffaa89
                     /ApEinfo_revcolor=#deff79
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
      901 cacctattgg tcttactgac atccactttg cctttctctc cacagggaaa aaaCAATTGA
      961 CAAGTTTGTA CAAAAAAGCA GGCTCCGAAT TcACCGGTgc cgccaccATG CTTCTGCAAC
     1021 CGGCGCCATG TGCACCTTCA GCAGGTTTTC CAAGACCGCT TGCTGCGCCA GGCGCTATGC
     1081 ACGGCAGCCA AAAAGACACC ACGTTTACAA AGATATTCGT CGGTGGACTC CCTTATCATA
     1141 CTACTGACGC GTCCCTTAGA AAGTACTTCG AGGGTTTCGG GGATATTGAA GAAGCCGTCG
     1201 TCATAACGGA TCGGCAAACT GGAAAGAGCC GGGGCTACGG TTTTGTCACG ATGGCTGATA
     1261 GGGCAGCGGC AGAGAGGGCT TGTAAAGACC CAAACCCTAT TATCGATGGG CGCAAGGCAA
     1321 ACGTAAACCT GGCATACCTT GGGGCAAAGC CGCGCTCATT GCAGACTGGC TTCGCTATCG
     1381 GCGTACAACA ACTCCACCCA ACATTGATTC AAAGAACTTA TGGACTCACA CCACATTACA
     1441 TTTATCCCCC AGCAATAGTA CAACCATCAG TCGTCATTCC TGCAGCTCCC GTTCCATCAC
     1501 TCTCCAGTCC TTATATAGAG TATACACCAG CCTCTCCAGC TTATGCCCAA TACCCCCCTG
     1561 CCACtTATGA CCAATACCCA TACGCCGCGA GCCCGGCCAC CGCAGCCAGC TTTGTAGGTT
     1621 ATAGTTACCC GGCTGCCGTT CCCCAAGCTC TCAGCGCTGC CGCTCCTGCT GGTACTACCT
     1681 TCGTACAATA CCAGGCTCCT CAGTTGCAGC CCGACAGAAT GCAAaacgtt atcgatGGCG
     1741 GAGGCGGATC TGATCCAAAA AAGAAGAGAA AGGTAGATCC AAAAAAGAAG AGAAAGGTAG
     1801 ATCCAAAAAA GAAGAGAAAG GTAGGATCTA CCGGATCTAG AaacgatGGT GGTGGTGGAA
     1861 GCGGGGGTGG GGGCAGCGGT GGAGGGGGAA GCgggcgcgc cATGGAGATG TGGCATGAAG
     1921 GCCTGGAAGA GGCATCTCGT TTGTACTTTG GGGAAAGGAA CGTGAAAGGC ATGTTTGAGG
     1981 TGCTGGAGCC CTTGCATGCT ATGATGGAAC GGGGCCCCCA GACTCTGAAG GAAACATCCT
     2041 TTAATCAGGC CTATGGTCGA GATTTAATGG AGGCCCAAGA GTGGTGCAGG AAGTACATGA
     2101 AATCAGGGAA TGTCAAGGAC CTCACCCAAG CCTGGGACCT CTATTATCAT GTGTTCCGAC
     2161 GAATCTCAAA GCAGCAGATC TCGTACGCGT CCCGGGGCGG TGGCTCATCT GGCGGAGGTG
     2221 GAGGCGGCGG TAGCGGAGGA GGCGGGTCCG GCGGCGGCGG TAGTgGGCCG GCCATGGACT
     2281 ACAAGGATCA CGACGGTGAC TATAAGGATC ATGACATCGA CTATAAGGAC GATGACGATA
     2341 AGtgaTTAAT TAAGAATTCG ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCCAGCACAG
     2401 TGGCGGCCGC TCGAGTCTAG AGGGCCCGCG GTTCGAAGGT AAGCCTATCC CTAACCCTCT
     2461 CCTCGGTCTC GATTCTACGC GTACCGGTTA GCAATTGttt tttcgatgag tttggacaaa
     2521 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     2581 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     2641 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     2701 gtggtactta agagggggag accaaagggc gagacgttaa ggcctcacgt gacatgtgag
     2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3301 aaagagttgg tagctcttga tccggcaaac aaaccacgct ggtagcggtg gtttttttgt
     3361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3421 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgccgtc
     3481 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     3541 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     3601 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     3661 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     3721 gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc
     3781 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     3841 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     3901 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     3961 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     4021 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     4081 cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     4141 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     4201 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     4261 atcttgttca atcataatat tattgaagca tttatcaggg ttcgtctcgt cccggtctcc
     4321 tcccatgcat g    
//
