LOCUS       Exported               11830 bp ds-DNA     circular SYN 04-OCT-2018
DEFINITION  no force control for F40-based desmoplakin II tension sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Anna-Lena Cost
  TITLE     Direct Submission
  JOURNAL   Exported Oct 4, 2018 from SnapGene 4.2.1
            http://www.snapgene.com
COMMENT     human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry].Stop
            
            Desmoplakin tension sensor is truncated after tension sensor module 
            integration to prevent force transduction and therefore account for 
            not-force related FRET changes.
FEATURES             Location/Qualifiers
     source          1..11830
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..589
                     /label=5' LTR
                     /note="5' MoMuSV LTR (long terminal repeat from Moloney 
                     murine sarcoma virus)"
                     /note="color: #f0e1e1"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
                     /note="color: #ffe4c4"
     misc_feature    659..1468
                     /label=-Psi(+)
                     /note="extended packaging signal"
                     /note="color: #f0e1e1"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
                     /note="color: #993366; direction: RIGHT"
     CDS             1565..2164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     enhancer        2339..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2843..2867
                     /label=pLPCX_seq_f
                     /note="pLPCX_seq_f"
                     /note="color: blue; sequence: AGCTcGTTTAGTGAACCGTCAGATC"
     misc_feature    2912..2917
                     /label=ECoRI
                     /note="ECoRI recognition site"
                     /note="color: #0080ff"
     CDS             2918..6976
                     /codon_start=1
                     /label=Desmoplakin II
                     /note="Human Desmoplakin II
                     
                     DSPII (NP_001008844.1)
                     
                     ---
                     Assigned domains are from Choi et al., Nature Structural 
                     Biology, 2012."
                     /note="This feature has 3 segments:
                        1: 2918 .. 6091 / #008000 / N-terminus: Armadilllofam...
                        2: 6092 .. 6958 / #008000 / Coiled-coiled-rod
                        3: 6959 .. 6976 / #008000 / C-terminus: Intermediate 
                     ..."
                     /translation="MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRR
                     GVITDQNSDGYCQTGTMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQLTRS
                     RELDECFAQANDQMEILDSLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRR
                     ASSKGGGGYTCQSGSGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHR
                     GIHNSIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSR
                     EIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQ
                     HPASDKIEAYMDTLQTQWSWILQITKCIDVHLKENAAYFQFFEEAQSTEAYLKGLQDSI
                     RKKYPCDKNMPLQHLLEQIKELEKEREKILEYKRQVQNLVNKSKKIVQLKPRNPDYRSN
                     KPIILRALCDYKQDQKIVHKGDECILKDNNERSKWYVTGPGGVDMLVPSVGLIIPPPNP
                     LAVDLSCKIEQYYEAILALWNQLYINMKSLVSWHYCMIDIEKIRAMTIAKLKTMRQEDY
                     MKTIADLELHYQEFIRNSQGSEMFGDDDKRKIQSQFTDAQKHYQTLVIQLPGYPQHQTV
                     TTTEITHHGTCQDVNHNKVIETNRENDKQETWMLMELQKIRRQIEHCEGRMTLKNLPLA
                     DQGSSHHITVKINELKSVQNDSQAIAEVLNQLKDMLANFRGSEKYCYLQNEVFGLFQKL
                     ENINGVTDGYLNSLCTVRALLQAILQTEDMLKVYEARLTEEETVCLDLDKVEAYRCGLK
                     KIKNDLNLKKSLLATMKTELQKAQQIHSQTSQQYPLYDLDLGKFGEKVTQLTDRWQRID
                     KQIDFRLWDLEKQIKQLRNYRDNYQAFCKWLYDAKRRQDSLESMKFGDSNTVMRFLNEQ
                     KNLHSEISGKRDKSEEVQKIAELCANSIKDYELQLASYTSGLETLLNIPIKRTMIQSPS
                     GVILQEAADVHARYIELLTRSGDYYRFLSEMLKSLEDLKLKNTKIEVLEEELRLARDAN
                     SENCNKNKFLDQNLQKYQAECSQFKAKLASLEELKRQAELDGKSAKQNLDKCYGQIKEL
                     NEKITRLTYEIEDEKRRRKSVEDRFDQQKNDYDQLQKARQCEKENLGWQKLESEKAIKE
                     KEYEIERLRVLLQEEGTRKREYENELAKASNRIQESKNQCTQVVQERESLLVKIKVLEQ
                     DKARLQRLEDELNRAKSTLEAETRVKQRLECEKQQIQNDLNQWKTQYSRKEEAIRKIES
                     EREKSEREKNSLRSEIERLQAEIKRIEERCRRKLEDSTRETQSQLETERSRYQREIDKL
                     RQRPYGSHRE"
     misc_feature    6977..6982
                     /label=dXhoI/dSalI
                     /note="color: #ff00ff"
     CDS             6983..8506
                     /codon_start=1
                     /label=F40-based tension sensor module
                     /note="F40-based tension sensor module sensitive to forces 
                     of 1-6 pN (Austen et al., Nature Cell Biology, 2015)"
                     /note="color: #800080"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGSGPGGAGPGGAGPGGAGPGGAGPGGAGPGGAGPGGAGPGGAGSMVSKGEEDN
                     MAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSP
                     QFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKV
                     KLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTY
                     KAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     CDS             6983..7666
                     /codon_start=1
                     /product="short version of YPet  
                     yellow fluorescent protein optimized for FRET with CyPet 
                     (Nguyen and Daugherty, Nature Biotechnology, 2005)"
                     /label=YPet (short)
                     /note="mammalian codon-optimized"
                     /note="color: #ffff7f"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAA"
     CDS             7673..7792
                     /codon_start=1
                     /label=F40
                     /note="Flagelliform linker peptide sensitive to 1-6 pN 
                     (Grashoff et al., Nature, 2010)"
                     /note="color: #ccffcc"
                     /translation="GPGGAGPGGAGPGGAGPGGAGPGGAGPGGAGPGGAGPGGA"
     misc_feature    7793..7798
                     /label=BamHI
                     /note="BamHI recognition site"
                     /note="color: #0080ff"
     CDS             7799..8506
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., Nature Biotechnology, 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #f05078"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    8510..8517
                     /label=NotI
                     /note="NotI recognition site"
                     /note="color: #0080ff"
     misc_feature    8524..8529
                     /label=ClaI
                     /note="ClaI recognition site"
                     /note="color: #0080ff"
     primer_bind     complement(8566..8591)
                     /label=pLPCX_seq_r
                     /note="pLPCX_seq_r"
                     /note="color: blue; sequence: ACCTACAGGTGGGGTCTTTCATTCCC"
     LTR             8569..9162
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
                     /note="color: #ffe4c4"
     protein_bind    complement(9415..9448)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(9508..9541)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     rep_origin      complement(9832..10420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10591..11451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10591 .. 11382 / #ccffcc
                        2: 11383 .. 11451 / #ccffcc / signal sequence
                     Cleavage site after base 11382"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11452..11556)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
     1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
     1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
     2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
     2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
     2881 ggactcagat ctgggcccat aaagcttata cgaattcatg agctgcaacg gaggctccca
     2941 cccgcggatc aacactctgg gccgcatgat ccgcgccgag tctggcccgg acctgcgcta
     3001 cgaggtgacc agcggcggcg ggggcaccag caggatgtac tattctcggc gcggcgtgat
     3061 caccgaccag aactcggacg gctactgtca aaccggcacg atgtccaggc accagaacca
     3121 gaacaccatc caggagctgc tgcagaactg ctccgactgc ttgatgcgag cagagctcat
     3181 cgtgcagcct gaattgaagt atggagatgg aatacaactg actcggagtc gagaattgga
     3241 tgagtgtttt gcccaggcca atgaccaaat ggaaatcctc gacagcttga tcagagagat
     3301 gcggcagatg ggccagccct gtgatgctta ccagaaaagg cttcttcagc tccaagagca
     3361 aatgcgagcc ctttataaag ccatcagtgt cccacgagtc cgcagggcca gctccaaggg
     3421 tggtggaggc tacacttgtc agagtggctc tggctgggat gagttcacca aacatgtcac
     3481 cagtgaatgt ttggggtgga tgaggcagca aagggcggag atggacatgg tggcctgggg
     3541 tgtggacctg gcctcagtgg agcagcacat taacagccac cggggcatcc acaactccat
     3601 cggcgactat cgctggcagc tggacaaaat caaagccgac ctgcgcgaga aatctgcgat
     3661 ctaccagttg gaggaggagt atgaaaacct gctgaaagcg tcctttgaga ggatggatca
     3721 cctgcgacag ctgcagaaca tcattcaggc cacgtccagg gagatcatgt ggatcaatga
     3781 ctgcgaggag gaggagctgc tgtacgactg gagcgacaag aacaccaaca tcgctcagaa
     3841 acaggaggcc ttctccatac gcatgagtca actggaagtt aaagaaaaag agctcaataa
     3901 gctgaaacaa gaaagtgacc aacttgtcct caatcagcat ccagcttcag acaaaattga
     3961 ggcctatatg gacactctgc agacgcagtg gagttggatt cttcagatca ccaagtgcat
     4021 tgatgttcat ctgaaagaaa atgctgccta ctttcagttt tttgaagagg cgcagtctac
     4081 tgaagcatac ctgaaggggc tccaggactc catcaggaag aagtacccct gcgacaagaa
     4141 catgcccctg cagcacctgc tggaacagat caaggagctg gagaaagaac gagagaaaat
     4201 ccttgaatac aagcgtcagg tgcagaactt ggtaaacaag tctaagaaga ttgtacagct
     4261 gaagcctcgt aacccagact acagaagcaa taaacccatt attctcagag ctctctgtga
     4321 ctacaaacaa gatcagaaaa tcgtgcataa gggggatgag tgtatcctga aggacaacaa
     4381 cgagcgcagc aagtggtacg tgacgggccc gggaggcgtt gacatgcttg ttccctctgt
     4441 ggggctgatc atccctcctc cgaacccact ggccgtggac ctctcttgca agattgagca
     4501 gtactacgaa gccatcttgg ctctgtggaa ccagctctac atcaacatga agagcctggt
     4561 gtcctggcac tactgcatga ttgacataga gaagatcagg gccatgacaa tcgccaagct
     4621 gaaaacaatg cggcaggaag attacatgaa gacgatagcc gaccttgagt tacattacca
     4681 agagttcatc agaaatagcc aaggctcaga gatgtttgga gatgatgaca agcggaaaat
     4741 acagtctcag ttcaccgatg cccagaagca ttaccagacc ctggtcattc agctccctgg
     4801 ctatccccag caccagacag tgaccacaac tgaaatcact catcatggaa cctgccaaga
     4861 tgtcaaccat aataaagtaa ttgaaaccaa cagagaaaat gacaagcaag aaacatggat
     4921 gctgatggag ctgcagaaga ttcgcaggca gatagagcac tgcgagggca ggatgactct
     4981 caaaaacctc cctctagcag accaggggtc ttctcaccac atcacagtga aaattaacga
     5041 gcttaagagt gtgcagaatg attcacaagc aattgctgag gttctcaacc agcttaaaga
     5101 tatgcttgcc aacttcagag gttctgaaaa gtactgctat ttacagaatg aagtatttgg
     5161 actatttcag aaactggaaa atatcaatgg tgttacagat ggctacttaa atagcttatg
     5221 cacagtaagg gcactgctcc aggctattct ccaaacagaa gacatgttaa aggtttatga
     5281 agccaggctc actgaggagg aaactgtctg cctggacctg gataaagtgg aagcttaccg
     5341 ctgtggactg aagaaaataa aaaatgactt gaacttgaag aagtcgttgt tggccactat
     5401 gaagacagaa ctacagaaag cccagcagat ccactctcag acttcacagc agtatccact
     5461 ttatgatctg gacttgggca agttcggtga aaaagtcaca cagctgacag accgctggca
     5521 aaggatagat aaacagatcg actttagatt atgggacctg gagaaacaaa tcaagcaatt
     5581 gaggaattat cgtgataact atcaggcttt ctgcaagtgg ctctatgatg ctaaacgccg
     5641 ccaggattcc ttagaatcca tgaaatttgg agattccaac acagtcatgc ggtttttgaa
     5701 tgagcagaag aacttgcaca gtgaaatatc tggcaaacga gacaaatcag aggaagtaca
     5761 aaaaattgct gaactttgcg ccaattcaat taaggattat gagctccagc tggcctcata
     5821 cacctcagga ctggaaactc tgctgaacat acctatcaag aggaccatga ttcagtcccc
     5881 ttctggggtg attctgcaag aggctgcaga tgttcatgct cggtacattg aactacttac
     5941 aagatctgga gactattaca ggttcttaag tgagatgctg aagagtttgg aagatctgaa
     6001 gctgaaaaat accaagatcg aagttttgga agaggagctc agactggccc gagatgccaa
     6061 ctcggaaaac tgtaataaga acaaattcct ggatcagaac ctgcagaaat accaggcaga
     6121 gtgttcccag ttcaaagcga agcttgcgag cctggaggag ctgaagagac aggctgagct
     6181 ggatgggaag tcggctaagc aaaatctaga caagtgctac ggccaaataa aagaactcaa
     6241 tgagaagatc acccgactga cttatgagat tgaagatgaa aagagaagaa gaaaatctgt
     6301 ggaagacaga tttgaccaac agaagaatga ctatgaccaa ctgcagaaag caaggcaatg
     6361 tgaaaaggag aaccttggtt ggcagaaatt agagtctgag aaagccatca aggagaagga
     6421 gtacgagatt gaaaggttga gggttctact gcaggaagaa ggcacccgga agagagaata
     6481 tgaaaatgag ctggcaaagg catctaatag gattcaggaa tcaaagaatc agtgtactca
     6541 ggtggtacag gaaagagaga gccttctggt gaaaatcaaa gtcctggagc aagacaaggc
     6601 aaggctgcag aggctggagg atgagctgaa tcgtgcaaaa tcaactctag aggcagaaac
     6661 cagggtgaaa cagcgcctgg agtgtgagaa acagcaaatt cagaatgacc tgaatcagtg
     6721 gaagactcaa tattcccgca aggaggaggc tattaggaag atagaatcgg aaagagaaaa
     6781 gagtgagaga gagaagaaca gtcttaggag tgagatcgaa agactccaag cagagatcaa
     6841 gagaattgaa gagaggtgca ggcgtaagct ggaggattct accagggaga cacagtcaca
     6901 gttagaaaca gaacgctccc gatatcagag ggagattgat aaactcagac agcgcccata
     6961 tgggtcccat cgagaggtcg agatggtgag caaaggcgaa gagctgttca ccggcgtggt
     7021 gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg tgagcggcga
     7081 gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca ccaccggcaa
     7141 gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc agtgcttcgc
     7201 ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc ccgagggcta
     7261 cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc gggccgaggt
     7321 gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg acttcaagga
     7381 ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat
     7441 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc acaacatcga
     7501 ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     7561 cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca aggaccccaa
     7621 cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggat ccgggccagg
     7681 tggagcaggg ccaggtggtg cagggccagg tggtgcaggg ccaggtggtg cagggcccgg
     7741 tggtgcaggt ccaggtggtg caggtccagg tggtgctggt ccaggtggtg ctggatccat
     7801 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     7861 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     7921 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     7981 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     8041 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     8101 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     8161 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     8221 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     8281 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     8341 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     8401 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     8461 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgccta
     8521 tacatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
     8581 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
     8641 ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
     8701 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     8761 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     8821 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
     8881 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     8941 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     9001 acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
     9061 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     9121 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat
     9181 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gctgcctcgc
     9241 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     9301 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     9361 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt actgataact
     9421 tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg tccagccaag
     9481 ctagggccgc gatccggaac ccttaatata acttcgtata atgtatgcta tacgaagtta
     9541 tcagtactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     9601 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     9661 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9721 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9781 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9841 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9901 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9961 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10021 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10081 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10141 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10201 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10261 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    10321 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10381 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    10441 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    10501 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    10561 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10621 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    10681 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10741 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    10801 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10861 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
    10921 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10981 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    11041 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    11101 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    11161 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
    11221 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    11281 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    11341 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11401 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    11461 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    11521 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    11581 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    11641 gccctttcgt cttcaagaat tgctagcaat tgctagcaat tgctagcaat tcataccaga
    11701 tcaccgaaaa ctgtcctcca aatgtgtccc cctcacactc ccaaattcgc gggcttctgc
    11761 ctcttagacc actctaccct attccccaca ctcaccggag ccaaagccgc ggcccttccg
    11821 tttctttgct
//
