LOCUS       Exported               14711 bp ds-DNA     circular SYN 04-OCT-2018
DEFINITION  Desmoplakin II tension sensor (FL-based).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14711)
  AUTHORS   Anna-Lena Cost
  TITLE     Direct Submission
  JOURNAL   Exported Oct 4, 2018 from SnapGene 4.2.1
            http://www.snapgene.com
COMMENT     pLPCXmod + human desmoplakin II [YPet(short)-FL-mCherry] 
            (internal-1353)
            
            desmoplakin II tension sensor sensitive to 3-5 pN
FEATURES             Location/Qualifiers
     source          1..14711
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..589
                     /label=5' LTR
                     /note="5' MoMuSV LTR (long terminal repeat from Moloney 
                     murine sarcoma virus)"
                     /note="color: #f0e1e1"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
                     /note="color: #ffe4c4"
     misc_feature    659..1468
                     /label=-Psi(+)
                     /note="extended packaging signal"
                     /note="color: #f0e1e1"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
                     /note="color: #993366; direction: RIGHT"
     CDS             1565..2164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     enhancer        2339..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2843..2867
                     /label=pLPCX_seq_f
                     /note="pLPCX_seq_f"
                     /note="color: blue; sequence: AGCTcGTTTAGTGAACCGTCAGATC"
     misc_feature    2912..2917
                     /label=ECoRI
                     /note="ECoRI recognition site"
                     /note="color: #0080ff"
     CDS             join(2921..6979,8645..11401)
                     /codon_start=1
                     /label=Desmoplakin II
                     /note="Human Desmoplakin II
                     
                     DSPII (NP_001008844.1)
                     
                     ---
                     Assigned domains are from Choi et al., Nature Structural 
                     Biology, 2012."
                     /note="This feature has 4 segments:
                        1:  2921 ..  6094 / #008000 / N-terminus: Armadilllof...
                        2:  6095 ..  6961 / #008000 / Coiled-coiled-rod
                        3:  6962 ..  6979 / #008000 / C-terminus: Intermediat...
                        4:  8645 .. 11401 / #008000 / C-terminus: 
                     Intermediat..."
                     /translation="MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRR
                     GVITDQNSDGYCQTGTMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQLTRS
                     RELDECFAQANDQMEILDSLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRR
                     ASSKGGGGYTCQSGSGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHR
                     GIHNSIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSR
                     EIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQ
                     HPASDKIEAYMDTLQTQWSWILQITKCIDVHLKENAAYFQFFEEAQSTEAYLKGLQDSI
                     RKKYPCDKNMPLQHLLEQIKELEKEREKILEYKRQVQNLVNKSKKIVQLKPRNPDYRSN
                     KPIILRALCDYKQDQKIVHKGDECILKDNNERSKWYVTGPGGVDMLVPSVGLIIPPPNP
                     LAVDLSCKIEQYYEAILALWNQLYINMKSLVSWHYCMIDIEKIRAMTIAKLKTMRQEDY
                     MKTIADLELHYQEFIRNSQGSEMFGDDDKRKIQSQFTDAQKHYQTLVIQLPGYPQHQTV
                     TTTEITHHGTCQDVNHNKVIETNRENDKQETWMLMELQKIRRQIEHCEGRMTLKNLPLA
                     DQGSSHHITVKINELKSVQNDSQAIAEVLNQLKDMLANFRGSEKYCYLQNEVFGLFQKL
                     ENINGVTDGYLNSLCTVRALLQAILQTEDMLKVYEARLTEEETVCLDLDKVEAYRCGLK
                     KIKNDLNLKKSLLATMKTELQKAQQIHSQTSQQYPLYDLDLGKFGEKVTQLTDRWQRID
                     KQIDFRLWDLEKQIKQLRNYRDNYQAFCKWLYDAKRRQDSLESMKFGDSNTVMRFLNEQ
                     KNLHSEISGKRDKSEEVQKIAELCANSIKDYELQLASYTSGLETLLNIPIKRTMIQSPS
                     GVILQEAADVHARYIELLTRSGDYYRFLSEMLKSLEDLKLKNTKIEVLEEELRLARDAN
                     SENCNKNKFLDQNLQKYQAECSQFKAKLASLEELKRQAELDGKSAKQNLDKCYGQIKEL
                     NEKITRLTYEIEDEKRRRKSVEDRFDQQKNDYDQLQKARQCEKENLGWQKLESEKAIKE
                     KEYEIERLRVLLQEEGTRKREYENELAKASNRIQESKNQCTQVVQERESLLVKIKVLEQ
                     DKARLQRLEDELNRAKSTLEAETRVKQRLECEKQQIQNDLNQWKTQYSRKEEAIRKIES
                     EREKSEREKNSLRSEIERLQAEIKRIEERCRRKLEDSTRETQSQLETERSRYQREIDKL
                     RQRPYGSHRETQTECEWTVDTSKLVFDGLRKKVTAMQLYECQLIDKTTLDKLLKGKKSV
                     EEVASEIQPFLRGAGSIAGASASPKEKYSLVEAKRKKLISPESTVMLLEAQAATGGIID
                     PHRNEKLTVDSAIARDLIDFDDRQQIYAAEKAITGFDDPFSGKTVSVSEAIKKNLIDRE
                     TGMRLLEAQIASGGVVDPVNSVFLPKDVALARGLIDRDLYRSLNDPRDSQKNFVDPVTK
                     KKVSYVQLKERCRIEPHTGLLLLSVQKRSMSFQGIRQPVTVTELVDSGILRPSTVNELE
                     SGQISYDEVGERIKDFLQGSSCIAGIYNETTKQKLGIYEAMKIGLVRPGTALELLEAQA
                     ATGFIVDPVSNLRLPVEEAYKRGLVGIEFKEKLLSAERAVTGYNDPETGNIISLFQAMN
                     KELIEKGHGIRLLEAQIATGGIIDPKESHRLPVDIAYKRGYFNEELSEILSDPSDDTKG
                     FFDPNTEENLTYLQLKERCIKDEETGLCLLPLKEKKKQVQTSQKNTLRKRRVVIVDPET
                     NKEMSVQEAYKKGLIDYETFKELCEQECEWEEITITGSDGSTRVVLVDRKTGSQYDIQD
                     AIDKGLVDRKFFDQYRSGSLSLTQFADMISLKNGVGTSSSMGSGVSDDVFSSSRHESVS
                     KISTISSVRNLTIRSSSFSDTLEESSPIAAIFDTENLEKISITEGIERGIVDSITGQRL
                     LEAQACTGGIIHPTTGQKLSLQDAVSQGVIDQDMATRLKPAQKAFIGFEGVKGKKKMSA
                     AEAVKEKWLPYEAGQRFLEFQYLTGGLVDPEVHGRISTEEAIRKGFIDGRAAQRLQDTS
                     SYAKILTCPKTKLKISYKDAINRSMVEDITGLRLLEAASVSSKGLPSPYNMSSAPGSRS
                     GSRSGSRSGSRSGSRSGSRRGSFDATGNSSYSYSYSFSSSSIGH"
     misc_feature    6980..6985
                     /label=dSalI/dXhoI
                     /note="XhoI recognition site"
                     /note="color: #808080"
     CDS             6986..8635
                     /codon_start=1
                     /label=FL-based tension sensor
                     /note="FL-based tension sensor module sensitive to 3-5 pN 
                     (Ringer et al., Nature Methods, 2017)"
                     /note="color: #800080"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGSMGEFDIRFRTDDDEQFEKVLKEMNRRARKDAGTVTYTRDGNDFEIRITGIS
                     EQNRKELAKEVERLAKEQNITVTYTERGSLEGSMVSKGEEDNMAIIKEFMRFKVHMEGS
                     VNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIP
                     DYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKK
                     TMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIK
                     LDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     CDS             6986..7669
                     /codon_start=1
                     /product="short version of YPet 
                     yellow fluorescent protein optimized for FRET with CyPet 
                     (Ngyuen and Daugherty, Nature Biotechnology, 2005)"
                     /label=YPet (short)
                     /note="mammalian codon-optimized"
                     /note="color: #ffff7f"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAA"
     misc_feature    7670..7675
                     /label=BamHI \\ BglII
                     /note="sites deleted"
                     /note="color: #ff80c0"
     CDS             7676..7921
                     /codon_start=1
                     /product="FL"
                     /label=FL
                     /note="Ferredoxin-like linker peptide is sensitive to 3-5 
                     pN (Ringer et al., Nature Methods, 2017)"
                     /note="color: #ccffff"
                     /translation="MGEFDIRFRTDDDEQFEKVLKEMNRRARKDAGTVTYTRDGNDFEI
                     RITGISEQNRKELAKEVERLAKEQNITVTYTERGSLE"
     CDS             7928..8635
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., Nature Biotechnology, 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #f05078"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    8636..8643
                     /label=NotI
                     /note="NotI recognition site"
                     /note="color: #0080ff"
     misc_feature    11402..11404
                     /label=STOP
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    11405..11410
                     /label=ClaI
                     /note="ClaI recognition site"
                     /note="color: #0080ff"
     primer_bind     complement(11447..11472)
                     /label=pLPCX_seq_r
                     /note="pLPCX_seq_r"
                     /note="color: blue; sequence: ACCTACAGGTGGGGTCTTTCATTCCC"
     LTR             11450..12043
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
                     /note="color: #ffe4c4"
     protein_bind    complement(12296..12329)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(12389..12422)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     rep_origin      complement(12713..13301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(13472..14332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 13472 .. 14263 / #ccffcc
                        2: 14264 .. 14332 / #ccffcc / signal sequence
                     Cleavage site after base 14263"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(14333..14437)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
     1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
     1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
     2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
     2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
     2881 ggactcagat ctgggcccat aaagcttata cgaattcacc atgagctgca acggaggctc
     2941 ccacccgcgg atcaacactc tgggccgcat gatccgcgcc gagtctggcc cggacctgcg
     3001 ctacgaggtg accagcggcg gcgggggcac cagcaggatg tactattctc ggcgcggcgt
     3061 gatcaccgac cagaactcgg acggctactg tcaaaccggc acgatgtcca ggcaccagaa
     3121 ccagaacacc atccaggagc tgctgcagaa ctgctccgac tgcttgatgc gagcagagct
     3181 catcgtgcag cctgaattga agtatggaga tggaatacaa ctgactcgga gtcgagaatt
     3241 ggatgagtgt tttgcccagg ccaatgacca aatggaaatc ctcgacagct tgatcagaga
     3301 gatgcggcag atgggccagc cctgtgatgc ttaccagaaa aggcttcttc agctccaaga
     3361 gcaaatgcga gccctttata aagccatcag tgtcccacga gtccgcaggg ccagctccaa
     3421 gggtggtgga ggctacactt gtcagagtgg ctctggctgg gatgagttca ccaaacatgt
     3481 caccagtgaa tgtttggggt ggatgaggca gcaaagggcg gagatggaca tggtggcctg
     3541 gggtgtggac ctggcctcag tggagcagca cattaacagc caccggggca tccacaactc
     3601 catcggcgac tatcgctggc agctggacaa aatcaaagcc gacctgcgcg agaaatctgc
     3661 gatctaccag ttggaggagg agtatgaaaa cctgctgaaa gcgtcctttg agaggatgga
     3721 tcacctgcga cagctgcaga acatcattca ggccacgtcc agggagatca tgtggatcaa
     3781 tgactgcgag gaggaggagc tgctgtacga ctggagcgac aagaacacca acatcgctca
     3841 gaaacaggag gccttctcca tacgcatgag tcaactggaa gttaaagaaa aagagctcaa
     3901 taagctgaaa caagaaagtg accaacttgt cctcaatcag catccagctt cagacaaaat
     3961 tgaggcctat atggacactc tgcagacgca gtggagttgg attcttcaga tcaccaagtg
     4021 cattgatgtt catctgaaag aaaatgctgc ctactttcag ttttttgaag aggcgcagtc
     4081 tactgaagca tacctgaagg ggctccagga ctccatcagg aagaagtacc cctgcgacaa
     4141 gaacatgccc ctgcagcacc tgctggaaca gatcaaggag ctggagaaag aacgagagaa
     4201 aatccttgaa tacaagcgtc aggtgcagaa cttggtaaac aagtctaaga agattgtaca
     4261 gctgaagcct cgtaacccag actacagaag caataaaccc attattctca gagctctctg
     4321 tgactacaaa caagatcaga aaatcgtgca taagggggat gagtgtatcc tgaaggacaa
     4381 caacgagcgc agcaagtggt acgtgacggg cccgggaggc gttgacatgc ttgttccctc
     4441 tgtggggctg atcatccctc ctccgaaccc actggccgtg gacctctctt gcaagattga
     4501 gcagtactac gaagccatct tggctctgtg gaaccagctc tacatcaaca tgaagagcct
     4561 ggtgtcctgg cactactgca tgattgacat agagaagatc agggccatga caatcgccaa
     4621 gctgaaaaca atgcggcagg aagattacat gaagacgata gccgaccttg agttacatta
     4681 ccaagagttc atcagaaata gccaaggctc agagatgttt ggagatgatg acaagcggaa
     4741 aatacagtct cagttcaccg atgcccagaa gcattaccag accctggtca ttcagctccc
     4801 tggctatccc cagcaccaga cagtgaccac aactgaaatc actcatcatg gaacctgcca
     4861 agatgtcaac cataataaag taattgaaac caacagagaa aatgacaagc aagaaacatg
     4921 gatgctgatg gagctgcaga agattcgcag gcagatagag cactgcgagg gcaggatgac
     4981 tctcaaaaac ctccctctag cagaccaggg gtcttctcac cacatcacag tgaaaattaa
     5041 cgagcttaag agtgtgcaga atgattcaca agcaattgct gaggttctca accagcttaa
     5101 agatatgctt gccaacttca gaggttctga aaagtactgc tatttacaga atgaagtatt
     5161 tggactattt cagaaactgg aaaatatcaa tggtgttaca gatggctact taaatagctt
     5221 atgcacagta agggcactgc tccaggctat tctccaaaca gaagacatgt taaaggttta
     5281 tgaagccagg ctcactgagg aggaaactgt ctgcctggac ctggataaag tggaagctta
     5341 ccgctgtgga ctgaagaaaa taaaaaatga cttgaacttg aagaagtcgt tgttggccac
     5401 tatgaagaca gaactacaga aagcccagca gatccactct cagacttcac agcagtatcc
     5461 actttatgat ctggacttgg gcaagttcgg tgaaaaagtc acacagctga cagaccgctg
     5521 gcaaaggata gataaacaga tcgactttag attatgggac ctggagaaac aaatcaagca
     5581 attgaggaat tatcgtgata actatcaggc tttctgcaag tggctctatg atgctaaacg
     5641 ccgccaggat tccttagaat ccatgaaatt tggagattcc aacacagtca tgcggttttt
     5701 gaatgagcag aagaacttgc acagtgaaat atctggcaaa cgagacaaat cagaggaagt
     5761 acaaaaaatt gctgaacttt gcgccaattc aattaaggat tatgagctcc agctggcctc
     5821 atacacctca ggactggaaa ctctgctgaa catacctatc aagaggacca tgattcagtc
     5881 cccttctggg gtgattctgc aagaggctgc agatgttcat gctcggtaca ttgaactact
     5941 tacaagatct ggagactatt acaggttctt aagtgagatg ctgaagagtt tggaagatct
     6001 gaagctgaaa aataccaaga tcgaagtttt ggaagaggag ctcagactgg cccgagatgc
     6061 caactcggaa aactgtaata agaacaaatt cctggatcag aacctgcaga aataccaggc
     6121 agagtgttcc cagttcaaag cgaagcttgc gagcctggag gagctgaaga gacaggctga
     6181 gctggatggg aagtcggcta agcaaaatct agacaagtgc tacggccaaa taaaagaact
     6241 caatgagaag atcacccgac tgacttatga gattgaagat gaaaagagaa gaagaaaatc
     6301 tgtggaagac agatttgacc aacagaagaa tgactatgac caactgcaga aagcaaggca
     6361 atgtgaaaag gagaaccttg gttggcagaa attagagtct gagaaagcca tcaaggagaa
     6421 ggagtacgag attgaaaggt tgagggttct actgcaggaa gaaggcaccc ggaagagaga
     6481 atatgaaaat gagctggcaa aggcatctaa taggattcag gaatcaaaga atcagtgtac
     6541 tcaggtggta caggaaagag agagccttct ggtgaaaatc aaagtcctgg agcaagacaa
     6601 ggcaaggctg cagaggctgg aggatgagct gaatcgtgca aaatcaactc tagaggcaga
     6661 aaccagggtg aaacagcgcc tggagtgtga gaaacagcaa attcagaatg acctgaatca
     6721 gtggaagact caatattccc gcaaggagga ggctattagg aagatagaat cggaaagaga
     6781 aaagagtgag agagagaaga acagtcttag gagtgagatc gaaagactcc aagcagagat
     6841 caagagaatt gaagagaggt gcaggcgtaa gctggaggat tctaccaggg agacacagtc
     6901 acagttagaa acagaacgct cccgatatca gagggagatt gataaactca gacagcgccc
     6961 atatgggtcc catcgagagg tcgagatggt gagcaaaggc gaagagctgt tcaccggcgt
     7021 ggtgcccatc ctggtggagc tggacggcga cgtgaacggc cacaagttca gcgtgagcgg
     7081 cgagggcgag ggcgacgcca cctacggcaa gctgaccctg aagctgctgt gcaccaccgg
     7141 caagctgccc gtgccctggc ccaccctggt gaccaccctg ggctacggcg tgcagtgctt
     7201 cgcccggtac cccgaccaca tgaagcagca cgacttcttc aagagcgcca tgcccgaggg
     7261 ctacgtgcag gagcggacca tcttcttcaa ggacgacggc aactacaaga cccgggccga
     7321 ggtgaagttc gagggcgaca ccctggtgaa ccggatcgag ctgaagggca tcgacttcaa
     7381 ggaggacggc aacatcctgg gccacaagct ggagtacaac tacaacagcc acaacgtgta
     7441 catcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc ggcacaacat
     7501 cgaggacggc ggcgtgcagc tggccgacca ctaccagcag aacaccccca tcggcgacgg
     7561 ccccgtgctg ctgcccgaca accactacct gagctaccag agcgccctgt tcaaggaccc
     7621 caacgagaag cgggaccaca tggtgctgct ggagttcctg accgccgccg gatctatggg
     7681 cgagtttgac atccggtttc ggactgatga cgacgaacag ttcgagaaag tgctgaagga
     7741 gatgaatcgt cgagccagaa aggatgctgg aactgtgacc tacacaaggg atgggaatga
     7801 cttcgagatt cgcattaccg gcataagcga gcaaaaccgc aaagaactgg ccaaagaggt
     7861 tgaaaggctt gcaaaggaac agaacatcac agtcacgtat accgagagag gttccctcga
     7921 aggatccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     7981 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     8041 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     8101 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     8161 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     8221 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     8281 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     8341 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     8401 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     8461 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     8521 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     8581 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaaggcggc
     8641 cgctacccag actgagtgtg agtggaccgt tgacacctcc aagctggtgt ttgatgggct
     8701 gaggaagaag gtgacagcaa tgcagctcta tgagtgtcag ctgatcgaca aaacaacctt
     8761 ggacaaacta ttgaagggga agaagtcagt ggaagaagtt gcttctgaaa tccagccatt
     8821 ccttcggggt gcaggatcta tcgctggagc atctgcttct cctaaggaaa aatactcttt
     8881 ggtagaggcc aagagaaaga aattaatcag cccagaatcc acagtcatgc ttctggaggc
     8941 ccaggcagct acaggtggta taattgatcc ccatcggaat gagaagctga ctgtggacag
     9001 tgccatagct cgggacctca ttgacttcga tgaccgtcag cagatatatg cagcagaaaa
     9061 agctatcact ggttttgatg atccattttc aggcaagaca gtatctgttt cagaagccat
     9121 caagaaaaat ttgattgata gagaaaccgg aatgcgcctg ctggaagccc agattgcttc
     9181 agggggtgta gtagaccctg tgaacagtgt ctttttgcca aaagatgtcg ccttggcccg
     9241 ggggctgatt gatagagatt tgtatcgttc cctgaatgat ccccgagata gtcagaaaaa
     9301 ctttgtggat ccagtcacca aaaagaaggt cagttacgtg cagctgaagg aacggtgcag
     9361 aatcgaacca catactggtc tgctcttgct ttcagtacag aagagaagca tgtccttcca
     9421 aggaatcaga caacctgtga ccgtcactga gctagtagat tctggtatat tgagaccgtc
     9481 cactgtcaat gaactggaat ctggtcagat ttcttatgac gaggttggtg agagaattaa
     9541 ggacttcctc cagggttcaa gctgcatagc aggcatatac aatgagacca caaaacagaa
     9601 gcttggcatt tatgaggcca tgaaaattgg cttagtccga cctggtactg ctctggagtt
     9661 gctggaagcc caagcagcta ctggctttat agtggatcct gttagcaact tgaggttacc
     9721 agtggaggaa gcctacaaga gaggtctggt gggcattgag ttcaaagaga agctcctgtc
     9781 tgcagaacga gctgtcactg ggtataatga tcctgaaaca ggaaacatca tctctttgtt
     9841 ccaagccatg aataaggaac tcatcgaaaa gggccacggt attcgcttat tagaagcaca
     9901 gatcgcaacc ggggggatca ttgacccaaa ggagagccat cgtttaccag ttgacatagc
     9961 atataagagg ggctatttca atgaggaact cagtgagatt ctctcagatc caagtgatga
    10021 taccaaagga ttttttgacc ccaacactga agaaaatctt acctatctgc aactaaaaga
    10081 aagatgcatt aaggatgagg aaacagggct ctgtcttctg cctctgaaag aaaagaagaa
    10141 acaggtgcag acatcacaaa agaataccct caggaagcgt agagtggtca tagttgaccc
    10201 agaaaccaat aaagaaatgt ctgttcagga ggcctacaag aagggcctaa ttgattatga
    10261 aaccttcaaa gaactgtgtg agcaggaatg tgaatgggaa gaaataacca tcacgggatc
    10321 agatggctcc accagggtgg tcctggtaga tagaaagaca ggcagtcagt atgatattca
    10381 agatgctatt gacaagggcc ttgttgacag gaagttcttt gatcagtacc gatccggcag
    10441 cctcagcctc actcaatttg ctgacatgat ctccttgaaa aatggtgtcg gcaccagcag
    10501 cagcatgggc agtggtgtca gcgatgatgt ttttagcagc tcccgacatg aatcagtaag
    10561 taagatttcc accatatcca gcgtcaggaa tttaaccata aggagcagct ctttttcaga
    10621 caccctggaa gaatcgagcc ccattgcagc catctttgac acagaaaacc tggagaaaat
    10681 ctccattaca gaaggtatag agcggggcat cgttgacagc atcacgggtc agaggcttct
    10741 ggaggctcag gcctgcacag gtggcatcat ccacccaacc acgggccaga agctgtcact
    10801 tcaggacgca gtctcccagg gtgtgattga ccaagacatg gccaccaggc tgaagcctgc
    10861 tcagaaagcc ttcataggct tcgagggtgt gaagggaaag aagaagatgt cagcagcaga
    10921 ggcagtgaaa gaaaaatggc tcccgtatga ggctggccag cgcttcctgg agttccagta
    10981 cctcacggga ggtcttgttg acccggaagt gcatgggagg ataagcaccg aagaagccat
    11041 ccggaagggg ttcatagatg gccgcgccgc acagaggctg caagacacca gcagctatgc
    11101 caaaatcctg acctgcccca aaaccaaatt aaaaatatcc tataaggatg ccataaatcg
    11161 ctccatggta gaagatatca ctgggctgcg ccttctggaa gccgcctccg tgtcgtccaa
    11221 gggcttaccc agcccttaca acatgtcttc ggctccgggg tcccgctccg gctcccgctc
    11281 gggatctcgc tccggatctc gctccgggtc ccgcagtggg tcccggagag gaagctttga
    11341 cgccacaggc aattcttcct actcttattc ctactcattt agcagtagtt ctattgggca
    11401 ctaaatcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
    11461 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
    11521 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
    11581 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
    11641 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
    11701 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
    11761 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
    11821 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
    11881 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
    11941 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
    12001 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
    12061 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg
    12121 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    12181 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    12241 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tactgataac
    12301 ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa
    12361 gctagggccg cgatccggaa cccttaatat aacttcgtat aatgtatgct atacgaagtt
    12421 atcagtactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    12481 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
    12541 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    12601 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    12661 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    12721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    12781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    12841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    12901 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    12961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    13021 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    13081 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    13141 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    13201 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    13261 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    13321 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    13381 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    13441 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    13501 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    13561 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    13621 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    13681 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    13741 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
    13801 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    13861 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    13921 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    13981 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    14041 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
    14101 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    14161 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    14221 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    14281 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    14341 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    14401 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    14461 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    14521 ggccctttcg tcttcaagaa ttgctagcaa ttgctagcaa ttgctagcaa ttcataccag
    14581 atcaccgaaa actgtcctcc aaatgtgtcc ccctcacact cccaaattcg cgggcttctg
    14641 cctcttagac cactctaccc tattccccac actcaccgga gccaaagccg cggcccttcc
    14701 gtttctttgc t
//
