LOCUS       Exported               11957 bp ds-DNA     circular SYN 04-OCT-2018
DEFINITION  donor only control for no force control of FL-based desmoplakin II 
            tension sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11957)
  AUTHORS   Anna-Lena Cost
  TITLE     Direct Submission
  JOURNAL   Exported Oct 4, 2018 from SnapGene 4.2.1
            http://www.snapgene.com
COMMENT     pLPCXmod + human desmoplakin 
            II(1-1353)-[YPet(short)-FL-mCherry(Y72L)].Stop
            
            donor only control for lifetime measurements of the no force control
            for FL-based desmoplakin tension sensor.
FEATURES             Location/Qualifiers
     source          1..11957
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..589
                     /label=5' LTR
                     /note="5' MoMuSV LTR (long terminal repeat from Moloney 
                     murine sarcoma virus)"
                     /note="color: #f0e1e1"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
                     /note="color: #ffe4c4"
     misc_feature    659..1468
                     /label=-Psi(+)
                     /note="extended packaging signal"
                     /note="color: #f0e1e1"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
                     /note="color: #993366; direction: RIGHT"
     CDS             1565..2164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     enhancer        2339..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2843..2867
                     /label=pLPCX_seq_f
                     /note="pLPCX_seq_f"
                     /note="color: blue; sequence: AGCTcGTTTAGTGAACCGTCAGATC"
     misc_feature    2912..2917
                     /label=ECoRI
                     /note="ECoRI recognition site"
                     /note="color: #0080ff"
     CDS             2918..6976
                     /codon_start=1
                     /label=Desmoplakin II
                     /note="Human Desmoplakin II
                     
                     DSPII (NP_001008844.1)
                     
                     ---
                     Assigned domains are from Choi et al., Nature Structural 
                     Biology, 2012."
                     /note="This feature has 3 segments:
                        1: 2918 .. 6091 / #008000 / N-terminus: Armadilllofam...
                        2: 6092 .. 6958 / #008000 / Coiled-coiled-rod
                        3: 6959 .. 6976 / #008000 / C-terminus: Intermediate 
                     ..."
                     /translation="MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRR
                     GVITDQNSDGYCQTGTMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQLTRS
                     RELDECFAQANDQMEILDSLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRR
                     ASSKGGGGYTCQSGSGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHR
                     GIHNSIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSR
                     EIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQ
                     HPASDKIEAYMDTLQTQWSWILQITKCIDVHLKENAAYFQFFEEAQSTEAYLKGLQDSI
                     RKKYPCDKNMPLQHLLEQIKELEKEREKILEYKRQVQNLVNKSKKIVQLKPRNPDYRSN
                     KPIILRALCDYKQDQKIVHKGDECILKDNNERSKWYVTGPGGVDMLVPSVGLIIPPPNP
                     LAVDLSCKIEQYYEAILALWNQLYINMKSLVSWHYCMIDIEKIRAMTIAKLKTMRQEDY
                     MKTIADLELHYQEFIRNSQGSEMFGDDDKRKIQSQFTDAQKHYQTLVIQLPGYPQHQTV
                     TTTEITHHGTCQDVNHNKVIETNRENDKQETWMLMELQKIRRQIEHCEGRMTLKNLPLA
                     DQGSSHHITVKINELKSVQNDSQAIAEVLNQLKDMLANFRGSEKYCYLQNEVFGLFQKL
                     ENINGVTDGYLNSLCTVRALLQAILQTEDMLKVYEARLTEEETVCLDLDKVEAYRCGLK
                     KIKNDLNLKKSLLATMKTELQKAQQIHSQTSQQYPLYDLDLGKFGEKVTQLTDRWQRID
                     KQIDFRLWDLEKQIKQLRNYRDNYQAFCKWLYDAKRRQDSLESMKFGDSNTVMRFLNEQ
                     KNLHSEISGKRDKSEEVQKIAELCANSIKDYELQLASYTSGLETLLNIPIKRTMIQSPS
                     GVILQEAADVHARYIELLTRSGDYYRFLSEMLKSLEDLKLKNTKIEVLEEELRLARDAN
                     SENCNKNKFLDQNLQKYQAECSQFKAKLASLEELKRQAELDGKSAKQNLDKCYGQIKEL
                     NEKITRLTYEIEDEKRRRKSVEDRFDQQKNDYDQLQKARQCEKENLGWQKLESEKAIKE
                     KEYEIERLRVLLQEEGTRKREYENELAKASNRIQESKNQCTQVVQERESLLVKIKVLEQ
                     DKARLQRLEDELNRAKSTLEAETRVKQRLECEKQQIQNDLNQWKTQYSRKEEAIRKIES
                     EREKSEREKNSLRSEIERLQAEIKRIEERCRRKLEDSTRETQSQLETERSRYQREIDKL
                     RQRPYGSHRE"
     misc_feature    6977..6982
                     /label=dXhoI/dSalI
                     /note="color: #ff00ff"
     CDS             6983..8632
                     /codon_start=1
                     /label=FL-based tension sensor module with dead mCherry
                     /note="FL-based tension sensor module sensitive to 3-5 pN 
                     (Ringer et al., Nature Methods, 2017)
                     with Y72L to disrupt chromophore formation (Yamashita et 
                     al., Scientific Reports, 2016)"
                     /note="color: #800080"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGSMGEFDIRFRTDDDEQFEKVLKEMNRRARKDAGTVTYTRDGNDFEIRITGIS
                     EQNRKELAKEVERLAKEQNITVTYTERGSLEGSMVSKGEEDNMAIIKEFMRFKVHMEGS
                     VNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMLGSKAYVKHPADIP
                     DYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKK
                     TMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIK
                     LDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     CDS             6983..7666
                     /codon_start=1
                     /product="short version of YPet  
                     yellow fluorescent protein optimized for FRET with CyPet 
                     (Nguyen and Daugherty, Nature Biotechnology, 2005)"
                     /label=YPet (short)
                     /note="mammalian codon-optimized"
                     /note="color: #ffff7f"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAA"
     misc_feature    7667..7672
                     /label=dBamHI/BglII
                     /note="sites deleted"
                     /note="color: #ff80c0"
     CDS             7673..7918
                     /codon_start=1
                     /product="FL"
                     /label=FL
                     /note="Ferredoxin-like linker peptide is sensitive to 3-5 
                     pN (Ringer et al., Nature Methods, 2017)"
                     /note="color: #ccffff"
                     /translation="MGEFDIRFRTDDDEQFEKVLKEMNRRARKDAGTVTYTRDGNDFEI
                     RITGISEQNRKELAKEVERLAKEQNITVTYTERGSLE"
     CDS             7925..8632
                     /codon_start=1
                     /product="mCherry: monomeric derivative of DsRed 
                     fluorescent protein (Shaner et al., Nature Biotechnology, 
                     2004)
                     
                     Y72L to disrupt chromophore formation and generate a 
                     fluorescently inactive ""dead"" mCherry version
                        (mutation to Leucin used in Yamashita et al., Scientific
                     Reports, 2016)"
                     /label=dead mCherry
                     /note="color: #a6acb3"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMLGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    8633..8635
                     /label=STOP
                     /note="STOP"
                     /note="color: #ff0000"
     misc_feature    8637..8644
                     /label=NotI
                     /note="NotI"
                     /note="color: #3366ff"
     primer_bind     complement(8693..8718)
                     /label=pLPCX_seq_r
                     /note="pLPCX_seq_r"
                     /note="color: blue; sequence: ACCTACAGGTGGGGTCTTTCATTCCC"
     LTR             8696..9289
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
                     /note="color: #ffe4c4"
     protein_bind    complement(9542..9575)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(9635..9668)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     rep_origin      complement(9959..10547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10718..11578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10718 .. 11509 / #ccffcc
                        2: 11510 .. 11578 / #ccffcc / signal sequence
                     Cleavage site after base 11509"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11579..11683)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
     1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
     1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
     2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
     2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
     2881 ggactcagat ctgggcccat aaagcttata cgaattcatg agctgcaacg gaggctccca
     2941 cccgcggatc aacactctgg gccgcatgat ccgcgccgag tctggcccgg acctgcgcta
     3001 cgaggtgacc agcggcggcg ggggcaccag caggatgtac tattctcggc gcggcgtgat
     3061 caccgaccag aactcggacg gctactgtca aaccggcacg atgtccaggc accagaacca
     3121 gaacaccatc caggagctgc tgcagaactg ctccgactgc ttgatgcgag cagagctcat
     3181 cgtgcagcct gaattgaagt atggagatgg aatacaactg actcggagtc gagaattgga
     3241 tgagtgtttt gcccaggcca atgaccaaat ggaaatcctc gacagcttga tcagagagat
     3301 gcggcagatg ggccagccct gtgatgctta ccagaaaagg cttcttcagc tccaagagca
     3361 aatgcgagcc ctttataaag ccatcagtgt cccacgagtc cgcagggcca gctccaaggg
     3421 tggtggaggc tacacttgtc agagtggctc tggctgggat gagttcacca aacatgtcac
     3481 cagtgaatgt ttggggtgga tgaggcagca aagggcggag atggacatgg tggcctgggg
     3541 tgtggacctg gcctcagtgg agcagcacat taacagccac cggggcatcc acaactccat
     3601 cggcgactat cgctggcagc tggacaaaat caaagccgac ctgcgcgaga aatctgcgat
     3661 ctaccagttg gaggaggagt atgaaaacct gctgaaagcg tcctttgaga ggatggatca
     3721 cctgcgacag ctgcagaaca tcattcaggc cacgtccagg gagatcatgt ggatcaatga
     3781 ctgcgaggag gaggagctgc tgtacgactg gagcgacaag aacaccaaca tcgctcagaa
     3841 acaggaggcc ttctccatac gcatgagtca actggaagtt aaagaaaaag agctcaataa
     3901 gctgaaacaa gaaagtgacc aacttgtcct caatcagcat ccagcttcag acaaaattga
     3961 ggcctatatg gacactctgc agacgcagtg gagttggatt cttcagatca ccaagtgcat
     4021 tgatgttcat ctgaaagaaa atgctgccta ctttcagttt tttgaagagg cgcagtctac
     4081 tgaagcatac ctgaaggggc tccaggactc catcaggaag aagtacccct gcgacaagaa
     4141 catgcccctg cagcacctgc tggaacagat caaggagctg gagaaagaac gagagaaaat
     4201 ccttgaatac aagcgtcagg tgcagaactt ggtaaacaag tctaagaaga ttgtacagct
     4261 gaagcctcgt aacccagact acagaagcaa taaacccatt attctcagag ctctctgtga
     4321 ctacaaacaa gatcagaaaa tcgtgcataa gggggatgag tgtatcctga aggacaacaa
     4381 cgagcgcagc aagtggtacg tgacgggccc gggaggcgtt gacatgcttg ttccctctgt
     4441 ggggctgatc atccctcctc cgaacccact ggccgtggac ctctcttgca agattgagca
     4501 gtactacgaa gccatcttgg ctctgtggaa ccagctctac atcaacatga agagcctggt
     4561 gtcctggcac tactgcatga ttgacataga gaagatcagg gccatgacaa tcgccaagct
     4621 gaaaacaatg cggcaggaag attacatgaa gacgatagcc gaccttgagt tacattacca
     4681 agagttcatc agaaatagcc aaggctcaga gatgtttgga gatgatgaca agcggaaaat
     4741 acagtctcag ttcaccgatg cccagaagca ttaccagacc ctggtcattc agctccctgg
     4801 ctatccccag caccagacag tgaccacaac tgaaatcact catcatggaa cctgccaaga
     4861 tgtcaaccat aataaagtaa ttgaaaccaa cagagaaaat gacaagcaag aaacatggat
     4921 gctgatggag ctgcagaaga ttcgcaggca gatagagcac tgcgagggca ggatgactct
     4981 caaaaacctc cctctagcag accaggggtc ttctcaccac atcacagtga aaattaacga
     5041 gcttaagagt gtgcagaatg attcacaagc aattgctgag gttctcaacc agcttaaaga
     5101 tatgcttgcc aacttcagag gttctgaaaa gtactgctat ttacagaatg aagtatttgg
     5161 actatttcag aaactggaaa atatcaatgg tgttacagat ggctacttaa atagcttatg
     5221 cacagtaagg gcactgctcc aggctattct ccaaacagaa gacatgttaa aggtttatga
     5281 agccaggctc actgaggagg aaactgtctg cctggacctg gataaagtgg aagcttaccg
     5341 ctgtggactg aagaaaataa aaaatgactt gaacttgaag aagtcgttgt tggccactat
     5401 gaagacagaa ctacagaaag cccagcagat ccactctcag acttcacagc agtatccact
     5461 ttatgatctg gacttgggca agttcggtga aaaagtcaca cagctgacag accgctggca
     5521 aaggatagat aaacagatcg actttagatt atgggacctg gagaaacaaa tcaagcaatt
     5581 gaggaattat cgtgataact atcaggcttt ctgcaagtgg ctctatgatg ctaaacgccg
     5641 ccaggattcc ttagaatcca tgaaatttgg agattccaac acagtcatgc ggtttttgaa
     5701 tgagcagaag aacttgcaca gtgaaatatc tggcaaacga gacaaatcag aggaagtaca
     5761 aaaaattgct gaactttgcg ccaattcaat taaggattat gagctccagc tggcctcata
     5821 cacctcagga ctggaaactc tgctgaacat acctatcaag aggaccatga ttcagtcccc
     5881 ttctggggtg attctgcaag aggctgcaga tgttcatgct cggtacattg aactacttac
     5941 aagatctgga gactattaca ggttcttaag tgagatgctg aagagtttgg aagatctgaa
     6001 gctgaaaaat accaagatcg aagttttgga agaggagctc agactggccc gagatgccaa
     6061 ctcggaaaac tgtaataaga acaaattcct ggatcagaac ctgcagaaat accaggcaga
     6121 gtgttcccag ttcaaagcga agcttgcgag cctggaggag ctgaagagac aggctgagct
     6181 ggatgggaag tcggctaagc aaaatctaga caagtgctac ggccaaataa aagaactcaa
     6241 tgagaagatc acccgactga cttatgagat tgaagatgaa aagagaagaa gaaaatctgt
     6301 ggaagacaga tttgaccaac agaagaatga ctatgaccaa ctgcagaaag caaggcaatg
     6361 tgaaaaggag aaccttggtt ggcagaaatt agagtctgag aaagccatca aggagaagga
     6421 gtacgagatt gaaaggttga gggttctact gcaggaagaa ggcacccgga agagagaata
     6481 tgaaaatgag ctggcaaagg catctaatag gattcaggaa tcaaagaatc agtgtactca
     6541 ggtggtacag gaaagagaga gccttctggt gaaaatcaaa gtcctggagc aagacaaggc
     6601 aaggctgcag aggctggagg atgagctgaa tcgtgcaaaa tcaactctag aggcagaaac
     6661 cagggtgaaa cagcgcctgg agtgtgagaa acagcaaatt cagaatgacc tgaatcagtg
     6721 gaagactcaa tattcccgca aggaggaggc tattaggaag atagaatcgg aaagagaaaa
     6781 gagtgagaga gagaagaaca gtcttaggag tgagatcgaa agactccaag cagagatcaa
     6841 gagaattgaa gagaggtgca ggcgtaagct ggaggattct accagggaga cacagtcaca
     6901 gttagaaaca gaacgctccc gatatcagag ggagattgat aaactcagac agcgcccata
     6961 tgggtcccat cgagaggtcg agatggtgag caaaggcgaa gagctgttca ccggcgtggt
     7021 gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg tgagcggcga
     7081 gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca ccaccggcaa
     7141 gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc agtgcttcgc
     7201 ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc ccgagggcta
     7261 cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc gggccgaggt
     7321 gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg acttcaagga
     7381 ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat
     7441 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc acaacatcga
     7501 ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     7561 cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca aggaccccaa
     7621 cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggat ctatgggcga
     7681 gtttgacatc cggtttcgga ctgatgacga cgaacagttc gagaaagtgc tgaaggagat
     7741 gaatcgtcga gccagaaagg atgctggaac tgtgacctac acaagggatg ggaatgactt
     7801 cgagattcgc attaccggca taagcgagca aaaccgcaaa gaactggcca aagaggttga
     7861 aaggcttgca aaggaacaga acatcacagt cacgtatacc gagagaggtt ccctcgaagg
     7921 atccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     7981 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     8041 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     8101 gcccttcgcc tgggacatcc tgtcccctca gttcatgctc ggctccaagg cctacgtgaa
     8161 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     8221 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     8281 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     8341 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     8401 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     8461 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     8521 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     8581 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaggcgg
     8641 ccgcctatac atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     8701 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     8761 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     8821 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     8881 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     8941 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     9001 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     9061 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     9121 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     9181 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     9241 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
     9301 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggct
     9361 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9421 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9481 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtact
     9541 gataacttcg tataatgtat gctatacgaa gttattaggt ctgaagagga gtttacgtcc
     9601 agccaagcta gggccgcgat ccggaaccct taatataact tcgtataatg tatgctatac
     9661 gaagttatca gtactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     9721 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     9781 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9841 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9901 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9961 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    10021 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    10081 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10141 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10201 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10261 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10321 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10381 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10441 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10501 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10561 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10621 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10681 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10741 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10801 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10861 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10921 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10981 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
    11041 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11101 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11161 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11221 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11281 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
    11341 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11401 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11461 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11521 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11581 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11641 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11701 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    11761 tcacgaggcc ctttcgtctt caagaattgc tagcaattgc tagcaattgc tagcaattca
    11821 taccagatca ccgaaaactg tcctccaaat gtgtccccct cacactccca aattcgcggg
    11881 cttctgcctc ttagaccact ctaccctatt ccccacactc accggagcca aagccgcggc
    11941 ccttccgttt ctttgct
//
