LOCUS       Exported                9648 bp ds-DNA     circular SYN 09-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2018 from SnapGene Viewer 4.2.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1171..3410
                     /label=pCSDest
     misc_feature    complement(1..1149)
                     /label=beta-Globin intron
     misc_feature    complement(1209..3218)
                     /label=Untitled
     misc_feature    complement(1209..1802)
                     /label=mCherry Exon 2
                     /note="mCherry Exon 2"
     misc_feature    complement(1803..1824)
                     /label=3' splice site
                     /note="3' splice site"
     CDS             complement(1803..1822)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="LSFFFL"
     misc_feature    complement(1826..2094)
                     /label=Untitled
     misc_feature    complement(1851..1855)
                     /label=Branch Point?
                     /note="Branch Point?"
     misc_feature    complement(2090..2096)
                     /label=5' SS
                     /note="5' SS"
     misc_feature    complement(2095..2277)
                     /label=CFTR Exon 9
                     /note="CFTR Exon 9"
     misc_feature    complement(2145..2156)
                     /label=Splicing Silencer
                     /note="Splicing Silencer"
     misc_feature    complement(2209..2214)
                     /label=Splicing Enhancer
                     /note="Splicing Enhancer"
     misc_feature    complement(2264)
                     /label=T->A mutation to generate STOP
                     /note="T->A mutation to generate STOP"
     misc_feature    complement(2277..2280)
                     /label=3' SS
                     /note="3' SS"
     misc_feature    complement(2278..2373)
                     /label=Untitled
     misc_feature    complement(2278..2317)
                     /label=Polymorphic region
                     /note="Polymorphic region"
     misc_feature    complement(2292..2317)
                     /label=TDP43 binding site
                     /note="TDP43 binding site"
     misc_feature    complement(2339..2343)
                     /label=Branch Point
                     /note="Branch Point"
     misc_feature    complement(2377..2384)
                     /label=5' splice site
                     /note="5' splice site"
     misc_feature    complement(2383..2493)
                     /label=mCherry Exon 1
                     /note="mCherry Exon 1"
     misc_feature    complement(2506..3213)
                     /label=shsfGFP2
                     /note="shsfGFP2"
     misc_feature    complement(3219..3390)
                     /label=Untitled
     misc_feature    complement(3220..3390)
                     /label=IBB (RchI 2-59)
                     /note="IBB (RchI 2-59)"
     misc_feature    complement(3396..3405)
                     /label=Kozak
                     /note="Kozak"
     misc_feature    3427..4094
                     /label=BICMVp
     misc_signal     complement(3427..3495)
                     /label=CMVp_min2
     primer_bind     complement(3428..3451)
                     /label=Set 1
                     /note="748"
     misc_signal     3559..3968
                     /label=CAG enhancer
     misc_signal     3969..4094
                     /label=CMVp_min1
     primer_bind     4020..4040
                     /label=Set 1 (2)
                     /note="19"
     misc_feature    4131..4140
                     /label=Kozak
                     /note="Kozak"
     CDS             4143..4916
                     /label=Untitled
     misc_feature    4143..4916
                     /label=mTagBFP
                     /note="mTagBFP"
     misc_feature    4659..4661
                     /label=Untitled
     misc_feature    4920..4985
                     /label=P2A
     misc_feature    4986..6227
                     /label=TDP43
                     /note="TDP43"
     misc_feature    4998..5216
                     /label=Ub-like domain
                     /note="Ub-like domain"
     misc_feature    5298..5756
                     /label=RRM domains
                     /note="RRM domains"
     misc_feature    5301..5318
                     /label=RNP-2
                     /note="RNP-2"
     misc_feature    5418..5441
                     /label=RNP-1
                     /note="RNP-1"
     misc_feature    5424..5426
                     /label=Untitled
     misc_feature    5430..5432
                     /label=Untitled
     misc_feature    5562..5579
                     /label=RNP-2
                     /note="RNP-2"
     misc_feature    5565..5567
                     /label=Untitled
     misc_feature    5664..5687
                     /label=RNP-1
                     /note="RNP-1"
     misc_feature    5670..5672
                     /label=Untitled
     misc_feature    5676..5678
                     /label=Untitled
     misc_feature    5772..6227
                     /label=Low-complexity region
                     /note="Low-complexity region"
     misc_feature    5943..6029
                     /label=Highly conserved region
                     /note="Highly conserved region"
     misc_feature    6314..7462
                     /label=beta-Globin intron
     misc_feature    7641..8314
                     /label=pUC ori
     primer_bind     7682..7699
                     /label=Set 1 (3)
                     /note="583._MP1"
     CDS             complement(8459..9319)
                     /label=AmpR
     primer_bind     9499..9517
                     /label=Set 1 (4)
                     /note="283._pQE-fwd"
ORIGIN
        1 cgagttcata agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat
       61 ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa
      121 caaaatataa aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca
      181 gcagggggct gtttcatata ctgatgacct ctttatagcc acctttgttc atggcagcca
      241 gcatatggca tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt
      301 tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttcccacaca ctattgcaat
      361 gaaaataaat ttcctttatt acccagaagt cagatgctca aggggcttca tgatgtcccc
      421 ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccaggg cattggccac
      481 accagccacc accttctgat aggcagcctg cacctgagga gtgaattctt tgccaaaatg
      541 atgagacagc acaacaacca gcacgttgcc caggagctgt aggaaaaaga agaaggcatg
      601 aacatggtta gcagaggggc ccggtttgga ctcagagtat tttatcctca tctcaaacag
      661 tgtatatcat tgtaaccata aagagaaagg caggatgatt accaggatgt agttgtttct
      721 accaataaga atatttccac gccagccaga atttatatgc agaaatattc taccttatca
      781 tttaattata acaattgttc tctaaaactg tgctgaagta caatataatt accctgattg
      841 ccttgaaaaa aaagtgatta gagaaagtac ttacaatctg acaaataaac gaaagtgaat
      901 ttaaaaattc gttacaaatg caagctaaag tttaacgaaa aaagttacag aaaatgaaaa
      961 gaaaataaga ggagacaatg gttgtcaaca gagtagaaag tgaaagaaac aaaattatca
     1021 tgagggtcca tggtgataca agggacatct tcccattcta aacaacaccc tgaaaacttt
     1081 gccccctcca tataacatga atttttcaat agcgaaaaag aaagaacaat caagggtccc
     1141 caaactcacc ctgaagttct cagtctagag gaggatcaac cactttgtac aagaaagctg
     1201 aagctagttt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
     1261 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
     1321 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
     1381 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
     1441 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
     1501 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
     1561 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
     1621 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
     1681 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
     1741 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
     1801 ccctgaggaa aaaaaaggaa agggcatgct cgccatgtgc aagatacagt gttgaatgtg
     1861 gtgcaaaata taaagatgtg gaagaaaaag ccactgaaaa taatatgagg aaataaaaaa
     1921 ctagagtaaa ttatcagtgg aacaatttct attatacaag acactacacc catacattct
     1981 cctaatgctc atgtaagaat ttctccaaaa ataccttcca gcactacaaa ctagaaaaaa
     2041 aaagagacat ggacaccaaa ttaagttctt aatagtgaag aacaaaagaa ctaccttgcc
     2101 tgctccagtg gatccagcaa ccgccaacaa ctgtcctctt tctatcttga aattaatatc
     2161 tttcaggaca ggagtaccaa gaagtgagaa attactgaag aagaggctgt catcaccatt
     2221 agaagttttt ctattgttat tgttttgttt tgctttctca aattattccc caaatccctg
     2281 ttaaaaaaaa acacacacac acacacacac acacacatca aaaataaaag atgagtttgt
     2341 cagatatttt caatagatgc ttgttttatg taccatactc acctcgccct cgccctcgat
     2401 ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt
     2461 gatgatggcc atgttatcct cctcgccctt gctggttccg gtgcctccac tttcatcttc
     2521 acctttagtg ataccggctg ccgtcacgaa ctccttcagg accatgtgat cgcgcttctc
     2581 gttcgggtct ttgctcagcg tggtctgggt gctcagatag tggttgtccg gcaggcgcac
     2641 cgggccgtcg ccgatcgggg tgttctgctg tttgtggtcg gcttcctgct ggctgccgtc
     2701 ctcaacgttg tggcggatct tgaagttagc cttgatgccg ttcttctgct tgtcggcttc
     2761 gatacggacg ttgtggctgt tgtggttgta ctccagcttg tgacccagga tgttgccgtc
     2821 ctccttgaag tcgttgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     2881 cacctccgcg cgggtcttgt aggtgccgtc gtccttgaac tcgatggtgc gctcctggac
     2941 gtagccttcc ggcatggcgg acttgaagaa gtcgtgacgc ttcatgtggt ccgggtagcg
     3001 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtcggccacg gcaccggcag
     3061 cttgccggtg gtgcagatga acttcagggt cagcttgccc tcggtggcat cgccctcgcc
     3121 ctcgccacgc acgctgaact tgtggccgtt tacgtcgccg tccagctcga ctttgatagg
     3181 cacagtacct gtaaatagtt cctctccttt actggtacca tcatcaggga atgagcttac
     3241 atttctcctc ttcagcatct ggtcatcctt cttagctttc ctcagctcca cattgacctc
     3301 tattctgcga cgcctcattt ctgtactgtc ttttcccttg ttcttgaatc tgtgaagacg
     3361 ggcagctggt gtattagcat tctcgttggt ggatcccatg gtggcaccgg ccggtgaatt
     3421 ctccaggcga tctgacggtt cactaaacga gctctgctta tataggcctc ccaccgtaca
     3481 cgccacctcg acatactcga gtagttatta atagtaatca attacggggt cattagttca
     3541 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     3601 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3661 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3721 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3781 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3841 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3901 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     3961 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     4021 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     4081 ccgtcagatc cgctagggat cctctagtca gctgacgcgt taccaccggt gccaccatgg
     4141 gaagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
     4201 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     4261 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
     4321 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
     4381 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     4441 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     4501 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     4561 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
     4621 ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca
     4681 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
     4741 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
     4801 actgcgacct ccctagcaaa ctggggcaca agcttaattc cggactcaga tctcgagctc
     4861 aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagaggag
     4921 gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg
     4981 gacctatgtc tgaatatatt cgggtaaccg aagatgagaa cgatgagccc attgaaatac
     5041 catcggaaga cgatgggacg gtgctgctct ccacggttac agcccagttt ccaggggcgt
     5101 gtgggcttcg ctacaggaat ccagtgtctc agtgtatgag aggtgtccgg ctggtagaag
     5161 gaattctgca tgccccagat gctggctggg gaaatctggt gtatgttgtc aactatccaa
     5221 aagataacaa aagaaaaatg gatgagacag atgcttcatc agcagtgaaa gtgaaaagag
     5281 cagtccagaa aacatccgat ttaatagtgt tgggtctccc atggaaaaca accgaacagg
     5341 acctgaaaga gtattttagt acctttggag aagttcttat ggtgcaggtc aagaaagatc
     5401 ttaagactgg tcattcaaag gggtttggct ttgttcgttt tacggaatat gaaacacaag
     5461 tgaaagtaat gtcacagcga catatgatag atggacgatg gtgtgactgc aaacttccta
     5521 attctaagca aagccaagat gagcctttga gaagcagaaa agtgtttgtg gggcgctgta
     5581 cagaggacat gactgaggat gagctgcggg agttcttctc tcagtacggg gatgtgatgg
     5641 atgtcttcat ccccaagcca ttcagggcct ttgcctttgt tacatttgca gatgatcaga
     5701 ttgcgcagtc tctttgtgga gaggacttga tcattaaagg aatcagcgtt catatatcca
     5761 atgccgaacc taagcacaat agcaatagac agttagaaag aagtggaaga tttggtggta
     5821 atccaggtgg ctttgggaat cagggtggat ttggtaatag cagagggggt ggagctggtt
     5881 tgggaaacaa tcaaggtagt aatatgggtg gtgggatgaa ctttggtgcg ttcagcatta
     5941 atccagccat gatggctgcc gcccaggcag cactacagag cagttggggt atgatgggca
     6001 tgttagccag ccagcagaac cagtcaggcc catcgggtaa taaccaaaac caaggcaaca
     6061 tgcagaggga gccaaaccag gccttcggtt ctggaaataa ctcttatagt ggctctaatt
     6121 ctggtgcagc aattggttgg ggatcagcat ccaatgcagg gtcgggcagt ggttttaatg
     6181 gaggctttgg ctcaagcatg gattctaagt cttctggctg gggaatgtag actagaccca
     6241 gctttcttgt acaaagtggt tgatcctcta ctagtaagct tgcatgcgat atctctagac
     6301 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgaaaa
     6361 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
     6421 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
     6481 accattgtct cctcttattt tcttttcatt ttctgtaact tttttcgtta aactttagct
     6541 tgcatttgta acgaattttt aaattcactt tcgtttattt gtcagattgt aagtactttc
     6601 tctaatcact tttttttcaa ggcaatcagg gtaattatat tgtacttcag cacagtttta
     6661 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
     6721 gcgtggaaat attcttattg gtagaaacaa ctacatcctg gtaatcatcc tgcctttctc
     6781 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
     6841 gggcccctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     6901 gctggttgtt gtgctgtctc atcattttgg caaagaattc actcctcagg tgcaggctgc
     6961 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     7021 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     7081 gtaataaagg aaatttattt tcattgcaat agtgtgtggg aattttttgt gtctctcact
     7141 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     7201 tttggcaaca tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc
     7261 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     7321 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     7381 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     7441 tccctcttct cttatgaact cgactgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7501 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7561 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7621 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7681 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7741 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7801 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7861 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     7921 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8041 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8101 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8161 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8221 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8281 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8341 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8581 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9421 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     9481 taacctataa aaataggcgt atcacgaggc cctttcgtct tcactcgacg ctggtcgagc
     9541 tgatacttcc cgtccgccag gggacatgcc ggcgatgctg aaggtcgcgc gcattcccga
     9601 tgaagaggcc ggttaccgcc tgttgacctg gtgggacggg cagggcgc
//
