LOCUS       Exported               12213 bp ds-DNA     circular SYN 10-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 10, 2018 from SnapGene Viewer 4.2.5
            http://www.snapgene.com
COMMENT     [DEFINITION]: - - [LOCUS]: 394.pDestTol2pA2 - [ACCESSION]: -  - 
            [GENE]: 228..514 - [CDS]: complement(9252..10109)
FEATURES             Location/Qualifiers
     source          1..12213
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    514..530
                     /label=Tol2 3' TIR
     misc_feature    1097..1117
                     /label=attB4
     misc_feature    1118..2988
                     /label=zmitfa promoter
     misc_feature    2990..3013
                     /label=attB1
     misc_feature    3110..4447
                     /label=SPRED1
     misc_feature    4537..4560
                     /label=attB2
     misc_feature    4563..4758
                     /label=polyA
     misc_feature    4760..4780
                     /label=attB3
     misc_feature    4820..5015
                     /label=polyA
     misc_feature    6087..7325
                     /label=mitfa minigene
     misc_feature    7326..9194
                     /label=zmitfa promoter
     misc_feature    9924..9942
                     /label=Tol2 5' TIR
     misc_feature    10452..11134
                     /label=pUC ori
     misc_feature    11232..12089
                     /label=AmpR
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttgtga gtgctaacaa ctgggctgct
     1141 gtgccgctct ttcgaggaaa tgaaaggaac tctggcggcc tatgatgagt taggaattgt
     1201 ctgattggtg aatcatacat aggctaatgt gggaccagct gtagtcagtc ataagcacgt
     1261 gattctctcg aaattagttt ataaataaac ttcactaaac tccaaccgtt cattctgttt
     1321 tagatgttgc ctttaaactt gccttaagat gttaataaag ttgctttttg gttgttgaca
     1381 aagctaggca taggacgata accgttttca aggtataccc cggtttgaaa aagtcaaggg
     1441 tttaaaacca ccaatatttt ctgctatacc gttcctaagg tatatttaag attttttatt
     1501 tattttttat ttttttgttt tttaggacaa cagcattttc agcagaaaag agatacaaaa
     1561 atgcttttta aattgtaaag aaatctgttt ttgaaactaa tgaagacagc agaagtcgat
     1621 cattcattga aattattaag cttgacatgt tttctgttcc aaaatatttt ttcaatgttt
     1681 ctcaaaataa aatatattat gtttaaagtt tttgtttttt actaagacat taaaaaaaat
     1741 aatatatttt agagtggcaa tcacaatacc atcaaactgt gatattttta tcaaaggtta
     1801 tcataccatc agaatcttat accccatgcc tagacacagc tgttctctct atctctctat
     1861 gttaatgcag tttccactcc attgacgaga tggttctctg taccacatac tcatccccat
     1921 tttacaatag ctacatttgc tcaagatgta gtcaactcaa acaatgcaga gacaacaaac
     1981 tgttgactta tcttttctat cgtaattcag cccttttggg aaatgagcat caacacacaa
     2041 tttagagtgg aagttgggtt aaggcagact cattttttac acattacaac cagtgattat
     2101 gtcaattatt atttaaaaaa aattcagtac agtatatgca catgctactt tgtttatgat
     2161 tgttagcttt tttaactaca gttcatttct atgctttctg tgcaaatgca caacagttac
     2221 tgatttactc tgatgataat tgtgccagta catccaacaa caacaaaaaa aaaaaagact
     2281 tacaatcaag ccccaaagtg ttcatacgtc tggcaaattc tgacttaaag ttacttttat
     2341 tcaaccagct agtttttttt tgactgtaaa tgacacgtaa gataataaga tgatgtacat
     2401 gaggcatcat tgtagaaaaa aaaatgaata tgaatctgaa tatgaactgt acgtctgtga
     2461 ttttgtttgt gcgcacttac atacgtgtgc acaatatttc aactcttaag aaaaaaatgc
     2521 aattaccaaa ggcccatcag acaacaaagt tctctttcct atcttttgct cttcaacatt
     2581 tcagagataa ctccatctta aactgtcttg tttttagacc aacagtgcta gtgattgtat
     2641 gccggatgct gagaacaaac aatgttttat gcaggatgag tggaactaaa gatgaaacgt
     2701 tagttagccg taagccagtt tcagtttaga cactagtaac ccatcgtctc aaagaggctt
     2761 ttgtcgaatc ggaccggtga cgtcaaatga actttataaa ggcataaatt catcgtttgg
     2821 gtaaaaaaaa caatatgaga agagcaatcc cctcccaccc ccttaaatac attttgtgtc
     2881 ttttctttgg cattgacatc ttcagctggc caagacgact ggtcagttct tgcacatcac
     2941 ctaaattcac aaagggaata cttttttgaa gctaacacat agttgaacgc aagtttgtac
     3001 aaaaaagcag gcttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     3061 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gatgccacca tgagcgagga
     3121 gacggcgact tctgacaacg ataatagtta tgcacgagtg cgagctgtgg tgatgacccg
     3181 agatgactca agtggtggat ggttaccact tggagggagt ggactaagca gcgtcactgt
     3241 cttcaaagtc cctcatcagg aagagaatgg ctgtgctgac ttttttatcc gtggagagcg
     3301 actcagggac aaaatggtgg ttttggaatg tatgcttaaa aaagacctca tttataataa
     3361 ggtcactcca acatttcacc actggaagat tgatgacaaa aagtttggtc ttacgtttca
     3421 aagtcctgct gatgctaggg cttttgatag aggtatccga agagctatag aggatatttc
     3481 tcaaggatgc cccgaatcaa aaaatgaagc tgaaggggca gatgacttac aagcaaatga
     3541 agaggattct tccagttctc tagtgaagga tcaccttttt cagcaagaga cagttgttac
     3601 cagtgagcct tatagaagct caaatataag accttctccc tttgaagatc tgaatgccag
     3661 aagagtctac atgcaaagcc aagccaatca gataacattt ggtcagccag gcttggacat
     3721 tcagagcaga agtatggaat acgtacagcg gcaaatatcc aaggaatgtg gaagcctaaa
     3781 gtcccaaaat agggtccctt tgaaatcaat cagacatgtc agctttcaag atgaggatga
     3841 gattgtcaga ataaaccctc gagatatctt aatacgtcgc tatgcagact acagacatcc
     3901 tgacatgtgg aaaaatgact tggaaagaga tgatgctgat tccagtattc agttttctaa
     3961 accagacagt aaaaaatcag actatctgta ctcttgtggg gatgagacta agttaagttc
     4021 acccaaagac tctgtggtat ttaagacgca gccttcctca ttaaaaatta agaagtcaaa
     4081 acgaagaaaa gaggatggtg aacgttctcg ctgcgtatac tgccaggaaa ggtttaatca
     4141 tgaagaaaat gtcaggggaa aatgtcagga tgctccagac cctattaaaa gatgcatata
     4201 tcaagttagt tgcatgctct gtgcagagag catgttgtat cattgtatgt cagactcaga
     4261 gggagatttt tctgatccct gttcgtgtga cactagcgac gacaagttct gcttgcgatg
     4321 gttagccctg gtagctttgt ctttcattgt accatgtatg tgctgctacg tccctttgag
     4381 aatgtgccat cgctgtggtg aggcatgtgg ttgctgtggt gggaaacata aagctgctgg
     4441 attgtaatct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     4501 ggttaattgc gcgcttggcg taatcatggt catagcaccc agctttcttg tacaaagtgg
     4561 gggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     4621 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4681 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     4741 aggtgtggga ggttttttcc aactttatta tacaaagttg ataattcact ggccgtcgtt
     4801 ttacggtacc atcgatgatg atccagacat gataagatac attgatgagt ttggacaaac
     4861 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     4921 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4981 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     5041 tggtatggct gattatgatc ctctagatca gatctcagat tgtatccaaa atatcatttt
     5101 ttgtgtttta aagatgaaca aaggtctcag ggaattggaa cgacatgagg atgattaatt
     5161 aataaaaaaa aattcatttt tgggtgaact atccctaaaa tcaaagcaat ttgggcattt
     5221 tttttctaat tattatggag aacttgcatc acagctcttc cactagaaaa taaaacaaat
     5281 ttcagaaata ctgcagaaat acttttggac ttaatgttaa ataacaatgt aaaaagtttt
     5341 tccagggcat cgtggtggtg cagtgggtag cattgtcgcc ttacagcaag aaggtcgctg
     5401 gttcgagact tggttgggtc atttctgtgt ggagtttgca tgttctcccc atgttcgtgt
     5461 gggtttcctc cgggtgctcc agtttcacct acagcccaaa gacatgcgct ataggtgaat
     5521 tgggtaagct aaattgtcta aagtgaatgg tactgtggtg ggtgtttccc agtgatgggt
     5581 tgcagctgca agggcatccg ctggatttgt ttgttggcat aagttggcag ttcattcaac
     5641 tgtggcaacc cctgattaat aaaggggcta agccaaaaag aaaatgaatg actgaatgaa
     5701 agtgaatcca gttgtacatg cctagacatg tcctccaccc ttgcaagatg tacaccccaa
     5761 gaaatggagc tgcatttgag gacaacaata agccaagcct gcaaataaaa gacacctcaa
     5821 cagtttttat tcaagctact ctttatatca tccgcgagaa tccttcaaaa atcaactaat
     5881 tgtttacacg atttcattct tgactagact ctgacatgat aaatgaaaaa atatatacac
     5941 aagcacacta aatattctga aatacaagat taatacttta cgaaattgcg ctcttcatga
     6001 tctggatgta acactggtac ttacaagaca cacacacaca caaatctaat tgtctaactt
     6061 tggttcatga aatttagttg gcattgctaa cagccattgt catgttcgtc catactgctg
     6121 ctgccgctac tgttggaagg tactggggat ccggaggaca acagcgggtc gctgctgccc
     6181 accggagaca gggtgttgtc cataagcatg tcctccagct tggtggaggc cttgtttggg
     6241 ctgtcgtaga cccccggctc gccgtcctcc gtgggactgt cattgtagct gatggtgccg
     6301 ttgttgaggt ccagagtggt aggacgggac atgtcaggac tgggaaggtg tgggtacaaa
     6361 ttggatgtgc agtcccccat cccaggctct tgtttgattg ctcgagccac taactcagcg
     6421 gaataaaggc tggaagaagc tacaacggtg agtccatgag cacgggcttg catttcaagt
     6481 tcctgaatac ggagcaggag atgtctgttt gcgtgttcta gtctcttctg tctgttttcc
     6541 agctcttttg ctttctgctg ctctttctgc aatttcctaa tgtaatccac tgatgctttc
     6601 aggatggtgc ctttattcca cctcatgtct ggatcatttg acttgggaat taaagtcccc
     6661 agctccttaa ttctgtcatt gatattaaac cgccttcttc gttcaatgag gttgtggttg
     6721 tccttctttt gtctctcttt tgccagggct ctgacttctg cttctactgg aaagccatca
     6781 ggtctttgat aagagtcaaa cttgccacat ggtccagctt tgtccattat gtgcatcatg
     6841 cccggagatg gagtaacgga taattccctt ttgacggccg gcaggcttga ggggcaggag
     6901 ttactgatgg aaactccagc tggaggaaga gcatgattgc tgtacatgtc cagcaggttt
     6961 gctgaaactg gaatcgtgtt tgtcatttga agtcctgcat ccatgaaccc aagaatgtca
     7021 tcactgtagc ttgattccaa acttataatg tcttcaatta catcgtccat ctctttctca
     7081 cagttgaggg tgagaagggc catagggctg ttgggagcgc tggctccggg tcccggggtc
     7141 atgccgtgct cggcgggctg gctggggccg ggccctgtgc tggcctgtgg gctcagcttg
     7201 gctcccagtg cactggacag gtagtgcttc acctgctgcc tctggctctg ctggatgtgg
     7261 tacttcgagg gggtttccag gtgggtctga acctggtagt gactgtactc gagcatctcc
     7321 aacatgttca actatgtgtt agcttcaaaa aagtattccc tttgtgaatt taggtgatgt
     7381 gcaagaactg accagtcgtc ttggccagct gaagatgtca atgccaaaga aaagacacaa
     7441 aatgtattta agggggtggg aggggattgc tcttctcata ttgttttttt tacccaaacg
     7501 atgaatttat gcctttataa agttcatttg acgtcaccgg tccgattcga caaaagcctc
     7561 tttgagacga tgggttacta gtgtctaaac tgaaactggc ttacggctaa ctaacgtttc
     7621 atctttagtt ccactcatcc tgcataaaac attgtttgtt ctcagcatcc ggcatacaat
     7681 cactagcact gttggtctaa aaacaagaca gtttaagatg gagttatctc tgaaatgttg
     7741 aagagcaaaa gataggaaag agaactttgt tgtctgatgg gcctttggta attgcatttt
     7801 tttcttaaga gttgaaatat tgtgcacacg tatgtaagtg cgcacaaaca aaatcacaga
     7861 cgtacagttc atattcagat tcatattcat ttttttttct acaatgatgc ctcatgtaca
     7921 tcatcttatt atcttacgtg tcatttacag tcaaaaaaaa actagctggt tgaataaaag
     7981 taactttaag tcagaatttg ccagacgtat gaacactttg gggcttgatt gtaagtcttt
     8041 tttttttttg ttgttgttgg atgtactggc acaattatca tcagagtaaa tcagtaactg
     8101 ttgtgcattt gcacagaaag catagaaatg aactgtagtt aaaaaagcta acaatcataa
     8161 acaaagtagc atgtgcatat actgtactga atttttttta aataataatt gacataatca
     8221 ctggttgtaa tgtgtaaaaa atgagtctgc cttaacccaa cttccactct aaattgtgtg
     8281 ttgatgctca tttcccaaaa gggctgaatt acgatagaaa agataagtca acagtttgtt
     8341 gtctctgcat tgtttgagtt gactacatct tgagcaaatg tagctattgt aaaatgggga
     8401 tgagtatgtg gtacagagaa ccatctcgtc aatggagtgg aaactgcatt aacatagaga
     8461 gatagagaga acagctgtgt ctaggcatgg ggtataagat tctgatggta tgataacctt
     8521 tgataaaaat atcacagttt gatggtattg tgattgccac tctaaaatat attatttttt
     8581 ttaatgtctt agtaaaaaac aaaaacttta aacataatat attttatttt gagaaacatt
     8641 gaaaaaatat tttggaacag aaaacatgtc aagcttaata atttcaatga atgatcgact
     8701 tctgctgtct tcattagttt caaaaacaga tttctttaca atttaaaaag catttttgta
     8761 tctcttttct gctgaaaatg ctgttgtcct aaaaaacaaa aaaataaaaa ataaataaaa
     8821 aatcttaaat ataccttagg aacggtatag cagaaaatat tggtggtttt aaacccttga
     8881 ctttttcaaa ccggggtata ccttgaaaac ggttatcgtc ctatgcctag ctttgtcaac
     8941 aaccaaaaag caactttatt aacatcttaa ggcaagttta aaggcaacat ctaaaacaga
     9001 atgaacggtt ggagtttagt gaagtttatt tataaactaa tttcgagaga atcacgtgct
     9061 tatgactgac tacagctggt cccacattag cctatgtatg attcaccaat cagacaattc
     9121 ctaactcatc ataggccgcc agagttcctt tcatttcctc gaaagagcgg cacagcagcc
     9181 cagttgttag cactgttgcc tcaaagcaag aacgtcactg gtccaagtct ctaacaggcc
     9241 agtccatggt tctgtgtgga gtttacatgt tgtcctcgtg ctcatgtgga tatcccctgg
     9301 gttctccggt tttctcctac agtccaaaaa cttgcgacat aggtgaattg aacaaaacca
     9361 aattagcata gacaagctcc taatcagcag aatatctctc cgtagcaatc tcttattcga
     9421 gatctgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg gactgtgctg
     9481 ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt gactgttata
     9541 atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg acatgacgtc
     9601 acttccaaag gaccaatgaa catgtctgac caatttcata taatgtgaaa acgattttca
     9661 taggcagaat aaataacatt taaattaaac tgggcatcag cgcaattcaa ttggtttggt
     9721 aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa
     9781 taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta
     9841 aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag
     9901 tacagtaatc aagtaaaatt actcaagtac tttacacctc tggttcttga ccccctacct
     9961 tcagcaagcc cagcagatcc actagttcta gagcggccgc caccgcggtg gagctccagc
    10021 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
    10081 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    10141 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    10201 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    10261 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    10321 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    10381 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    10441 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10501 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10561 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10621 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10681 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10741 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10801 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10861 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    10921 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10981 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    11041 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    11101 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    11161 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    11221 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    11281 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    11341 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    11401 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    11461 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    11521 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11581 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11641 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11701 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11761 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11821 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11881 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11941 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    12001 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    12061 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    12121 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    12181 ataggggttc cgcgcacatt tccccgaaaa gtg
//
