LOCUS       Exported               13960 bp ds-DNA     circular SYN 06-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSOMA-T679A-NLS
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Patrick Krysan
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2018 from SnapGene 2.8.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13960
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    22..663
                     /note="UBQ10 Promoter"
     CDS             673..3558
                     /codon_start=1
                     /product="FRET Biosensor"
                     /note="FRET Sensor CDS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYLEKFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSI
                     KKVWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSN
                     QLLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDRGTSAGGSAGGSAGGSAGG
                     SAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAG
                     GSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSA
                     GGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTS
                     AGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGPGSKKM
                     FWRSASWSASRTASQVPEGDEQSLNIPCAISSGPSRRCPAAPLAPRDHHNSKARACLPP
                     LQPLAISRRSLDEWPGGRMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
                     KLTLKFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQK
                     NGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFLTAAGITLGMDELSRLPKKKRKVGG"
     CDS             3529..3549
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     terminator      3574..3826
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4081..4105
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8466..9054)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10340..11134)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11559..11583
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11875..12900)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12968..13645)
                     /note="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 aattcgagct cggtacccgg ggatccttgt cgacgagtca gtaataaacg gcgtcaaagt
       61 ggttgcagcc ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca
      121 acctaaaaat aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg
      181 tgtcatttta ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct
      241 cgtcttttct tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt
      301 cagatttcaa tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag
      361 gtaaatttct gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa
      421 tttcgtatat gttctttggt ttagattctg ttaatcttag atcgaagacg attttctggg
      481 tttgatcgtt agatatcatc ttaattctcg attagggttt catagatatc atccgatttg
      541 ttcaaataat ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc
      601 tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta
      661 acagaattcg gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      721 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      781 gatgccacct acggcaagct gaccctgaag cttctatgca ccaccggcaa gctgcccgtg
      841 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
      901 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      961 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1021 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1081 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     1141 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     1201 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1261 cccgacaacc actacctgag ctaccagtcc gccctgttca aagaccccaa cgagaagcgc
     1321 gatcacatgg tcctgctgga gttcctgacc gccgccggga tcactgaggg catgaacgag
     1381 ctgtacctcg agaagttttc tcaagaacag atcggcgaaa acattgtgtg cagggtcatt
     1441 tgtaccacgg gtcaaattcc catccgagat ttgtcagctg atatttcaca agtgcttaag
     1501 gaaaaacgat ccataaagaa agtttggaca tttggtagaa acccagcctg tgactatcat
     1561 ttaggaaaca tttcaagact gtcaaataag catttccaaa tactactagg agaagacggt
     1621 aaccttttat tgaatgacat ttccactaat gggacctggt taaatgggca aaaagtcgag
     1681 aagaacagca atcagttact gtcccaaggt gatgaaataa ccgttggtgt aggcgtggaa
     1741 tcagatattt tatctctggt cattttcata aacgacaaat ttaagcagtg cctggagcag
     1801 aacaaagttg atcgcggtac cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     1861 gctggtggta gtgctggtgg ttccggcagt gctggtggta gtgctggtgg tagtaccagt
     1921 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggttcc
     1981 ggcagtgctg gtggtagtgc tggtggtagt accagtgctg gtggtagtgc tggtggtagt
     2041 gctggtggta gtgctggtgg tagtgctggt ggttccggca gtgctggtgg tagtgctggt
     2101 ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     2161 gctggtggtt ccggcagtgc tggtggtagt gctggtggta gtaccagtgc tggtggtagt
     2221 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggttccgg cagtgctggt
     2281 ggtagtgctg gtggtagtac cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     2341 gctggtggta gtgctggtgg ttccggcagt gctggtggta gtgctggtgg tagtaccagt
     2401 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggttcc
     2461 ggcagtgctg gtggtagtgc tggtggtagt accagtgctg gtggtagtgc tggtggtagt
     2521 gctggtggta gtgctggtgg tagtgctggt ggttccggac ctggttctaa gaaaatgttt
     2581 tggcggtctg cctcttggtc tgcttcacgg actgcatcac aagttcctga gggtgatgag
     2641 caaagcctga acattccgtg tgctattagt tctgggccga gtcgaagatg tccagctgct
     2701 cctttggcac ctcgtgatca tcataacagc aaggctagag cttgtttgcc accattgcag
     2761 cctcttgcca tttctaggag gagcttagac gagtggcctg gcggccgcat ggtgagcaag
     2821 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2881 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2941 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3001 ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     3061 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3121 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3181 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3241 aactacatca gcgggaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     3301 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag
     3361 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3421 cagtccgcct taagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3481 ttgaccgccg ccgggatcac tctcggcatg gacgagctgt ctagacttcc taagaagaag
     3541 agaaaggttg gaggatagtc gacgaatttc cccgatcgtt caaacatttg gcaataaagt
     3601 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     3661 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     3721 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     3781 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggag atctgtcgac
     3841 ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3901 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3961 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta
     4021 gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt
     4081 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataacggata
     4141 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     4201 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     4261 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg
     4321 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     4381 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     4441 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     4501 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     4561 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     4621 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     4681 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     4741 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     4801 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     4861 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     4921 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     4981 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     5041 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     5101 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     5161 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc
     5221 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     5281 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     5341 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     5401 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     5461 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     5521 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     5581 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     5641 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     5701 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     5761 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     5821 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     5881 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     5941 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     6001 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     6061 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     6121 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     6181 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     6241 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     6301 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     6361 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
     6421 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     6481 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     6541 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     6601 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     6661 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     6721 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     6781 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     6841 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     6901 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     6961 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     7021 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     7081 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     7141 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     7201 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     7261 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     7321 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     7381 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     7441 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     7501 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     7561 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt
     7621 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     7681 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     7741 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     7801 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     7861 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     7921 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     7981 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     8041 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     8101 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     8161 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8221 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8281 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8341 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8401 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8461 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8521 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8581 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8641 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8701 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8761 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8821 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8881 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     8941 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9001 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9061 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9121 ttggtcatgc attctaggga aggtgcgaac aagtccctga tatgagatca tgtttgtcat
     9181 ctggagccat agaacagggt tcatcatgag tcatcaactt accttcgccg acagtgaatt
     9241 cagcagtaag cgccgtcaga ccagaaaaga gattttcttg tcccgcatgg agcagattct
     9301 gccatggcaa aacatggtgg aagtcatcga gccgttttac cccaaggctg gtaatggccg
     9361 gcgaccttat ccgctggaaa ccatgctacg cattcactgc atgcagcatt ggtacaacct
     9421 gagcgatggc gcgatggaag atgctctgta cgaaatcgcc tccatgcgtc tgtttgcccg
     9481 gttatccctg gatagcgcct tgccggaccg caccaccatc atgaatttcc gccacctgct
     9541 ggagcagcat caactggccc gccaattgtt caagaccatc aatcgctggc tggccgaagc
     9601 aggcgtcatg atgactcaag gcaccttggt cgatgccacc atcattgagg cacccagctc
     9661 gaccaagaac aaagagcagc aacgcgatcc ggagatgcat cagaccaaga aaggcaatca
     9721 gtggcacttt ggcatgaagg cccacattgg tgtcgatgcc aagagtggcc tgacccacag
     9781 cctggtcacc accgcggcca acgagcatga cctcaatcag ctgggtaatc tgctgcatgg
     9841 agaggagcaa tttgtctcag ccgatgccgg ctaccaaggg gcgccacagc gcgaggagct
     9901 ggccgaggtg gatgtggact ggctgatcgc cgagcgcccc ggcaaggtaa gaaccttgaa
     9961 acagcatcca cgcaagaaca aaacggccat caacatcgaa tacatgaaag ccagcatccg
    10021 ggccagggtg gagcacccat ttcgcatcat caagcgacag ttcggcttcg tgaaagccag
    10081 atacaagggg ttgctgaaaa acgataacca actggcgatg ttattcacgc tggccaacct
    10141 gtttcgggcg gaccaaatga tacgtcagtg ggagagatct cactaaaaac tggggataac
    10201 gccttaaatg gcgaagaaac ggtctaaata ggctgattca aggcatttac gggagaaaaa
    10261 atcggctcaa acatgaagaa atgaaatgac tgagtcagcc gagaagaatt tccccgctta
    10321 ttcgcacctt ccctaggtac taaaacaatt catccagtaa aatataatat tttattttct
    10381 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
    10441 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc
    10501 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc
    10561 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat
    10621 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg
    10681 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat
    10741 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga
    10801 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct
    10861 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag
    10921 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc
    10981 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat
    11041 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt
    11101 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca
    11161 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
    11221 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
    11281 gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct
    11341 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
    11401 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
    11461 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
    11521 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
    11581 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt
    11641 aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt taggaattag
    11701 aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc
    11761 tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac
    11821 attattatgg agaaactcga gcttgtcgat cgacagatcc ggtcggcatc tactctattt
    11881 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
    11941 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
    12001 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
    12061 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg
    12121 tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
    12181 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
    12241 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
    12301 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
    12361 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
    12421 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
    12481 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
    12541 atccatagcc tccgcgaccg gttgtagaac agcgggcagt tcggtttcag gcaggtcttg
    12601 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc taaactcccc
    12661 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
    12721 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
    12781 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
    12841 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
    12901 atctcattgc cccccgggat ctgcgaaagc tcgagagaga tagatttgta gagagagact
    12961 ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaaggtcttg
    13021 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
    13081 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
    13141 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
    13201 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
    13261 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
    13321 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
    13381 ctccaccatg ttatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
    13441 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
    13501 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
    13561 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
    13621 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
    13681 attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata
    13741 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    13801 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    13861 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    13921 taacaatttc acacaggaaa cagctatgac catgattacg
//
