LOCUS       pCAG-TetR-RNF169UBD      8549 bp    DNA     circular UNA 12-DEC-2009
DEFINITION  
ACCESSION   pCAG-cre-bpA
DBSOURCE    GenBank
FEATURES             Location/Qualifiers
     promoter        38..1708
                     /note="Oriinal location: 87..1753"
                     /label="CAG Promoter"
                     /label=""CAG Promoter""
     CDS             1750..2373
                     /label=tetR
     CDS             2377..2451
                     /label="G4S linker"
     CDS             2452..3072
                     /label=tetR
     CDS             3073..3090
                     /label=NLS
     CDS             3097..3141
                     /label="G4S linker"
     CDS             3178..3201
                     /label=FLAG
     CDS             3202..3345
                     /label=RNF169UDB
     polyA_signal    3380..3651
                     /note="Oriinal location: 2900..3171"
                     /note="pA from bovine growth hormone"
                     /label=bpA
     promoter        3692..4791
                     /label="EF1a promoter"
     CDS             4823..5542
                     /label=BFP
     polyA_signal    5543..5774
                     /label=pGHpA
     CDS             6546..7406
                     /label=ampR
ORIGIN
        1 ACAGGAAACA GCTATGACCA TGAGGCGCGC CGGATTCGAC ATTGATTATT GACTAGTTAT
       61 TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA
      121 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA
      181 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG
      241 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG
      301 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC
      361 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTC
      421 GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC ACCCCCAATT
      481 TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCGGGGGG GGGGGGGGGG
      541 CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG GGGCGAGGCG GAGAGGTGCG
      601 GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT TTATGGCGAG GCGGCGGCGG
      661 CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGTC GCGCCTTCGC
      721 CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA
      781 CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT
      841 TAATGACGGC TCGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTAAAGGGCT CCGGGAGGGC
      901 CCTTTGTGCG GGGGGGAGCG GCTCGGGGGG TGCGTGCGTG TGTGTGTGCG TGGGGAGCGC
      961 CGCGTGCGGC CCGCGCTGCC CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC GGGGCTTTGT
     1021 GCGCTCCGCG TGTGCGCGAG GGGAGCGCGG CCGGGGGCGG TGCCCCGCGG TGCGGGGGGG
     1081 CTGCGAGGGG AACAAAGGCT GCGTGCGGGG TGTGTGCGTG GGGGGGTGAG CAGGGGGTGT
     1141 GGGCGCGGCG GTCGGGCTGT AACCCCCCCC TGCACCCCCC TCCCCGAGTT GCTGAGCACG
     1201 GCCCGGCTTC GGGTGCGGGG CTCCGTGCGG GGCGTGGCGC GGGGCTCGCC GTGCCGGGCG
     1261 GGGGGTGGCG GCAGGTGGGG GTGCCGGGCG GGGCGGGGCC GCCTCGGGCC GGGGAGGGCT
     1321 CGGGGGAGGG GCGCGGCGGC CCCGGAGCGC CGGCGGCTGT CGAGGCGCGG CGAGCCGCAG
     1381 CCATTGCCTT TTATGGTAAT CGTGCGAGAG GGCGCAGGGA CTTCCTTTGT CCCAAATCTG
     1441 GCGGAGCCGA AATCTGGGAG GCGCCGCCGC ACCCCCTCTA GCGGGCGCGG GCGAAGCGGT
     1501 GCGGCGCCGG CAGGAAGGAA ATGGGCGGGG AGGGCCTTCG TGCGTCGCCG CGCCGCCGTC
     1561 CCCTTCTCCA TCTCCAGCCT CGGGGCTGCC GCAGGGGGAC GGCTGCCTTC GGGGGGGACG
     1621 GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC TCTAGAGCCT CTGCTAACCA
     1681 TGTTCATGCC TTCTTCTTTT TCCTACAGAT CCTTAATTAA TAATAATACG ACTCACTATA
     1741 GGGGCCACCA TGTCCAGACT GGACAAGAGC AAAGTCATCA ACTCTGCCTT GGAGCTCCTG
     1801 AATGAAGTTG GCATTGAGGG CTTGACCACC AGGAAGCTGG CCCAGAAGCT GGGTGTGGAG
     1861 CAGCCTACCC TGTACTGGCA TGTGAAGAAC AAGAGGGCTC TGCTTGATGC CCTGGCCATT
     1921 GAGATGTTGG ACAGGCACCA CACCCACTTC TGCCCTCTGG AAGGGGAGTC CTGGCAGGAC
     1981 TTCCTGAGGA ACAATGCCAA GAGCTTCAGA TGTGCCTTGC TCTCCCACCG GGATGGTGCC
     2041 AAAGTTCACT TGGGCACCAG GCCTACAGAG AAGCAGTATG AGACCCTGGA GAACCAGCTG
     2101 GCATTCCTGT GCCAACAAGG CTTCTCCCTG GAAAATGCCT TGTATGCCCT CTCTGCTGTG
     2161 GGCCACTTCA CCTTGGGCTG TGTGCTGGAG GACCAGGAGC ACCAAGTTGC CAAGGAGGAG
     2221 AGGGAGACCC CCACCACTGA CTCCATGCCA CCACTGCTGC GGCAAGCTAT TGAGTTGTTT
     2281 GACCACCAAG GGGCTGAGCC TGCATTCCTT TTTGGCCTGG AACTGATCAT CTGTGGCCTG
     2341 GAAAAGCAGC TGAAATGTGA GTCTGGCTCT CACGTGGGAG GAGGTGGAAG TGGAGGTGGA
     2401 GGTAGCGGTG GAGGAGGTAG TGGAGGTGGT GGAAGCGGAG GAGGTGGTAG CATGTCCAGA
     2461 CTGGACAAGA GCAAAGTCAT CAACTCTGCC TTGGAGCTCC TGAATGAAGT TGGCATTGAG
     2521 GGCTTGACCA CCAGGAAGCT GGCCCAGAAG CTGGGTGTGG AGCAGCCTAC CCTGTACTGG
     2581 CATGTGAAGA ACAAGAGGGC TCTGCTTGAT GCCCTGGCCA TTGAGATGTT GGACAGGCAC
     2641 CACACCCACT TCTGCCCTCT GGAAGGGGAG TCCTGGCAGG ACTTCCTGAG GAACAATGCC
     2701 AAGAGCTTCA GATGTGCCTT GCTCTCCCAC CGGGATGGTG CCAAAGTTCA CTTGGGCACC
     2761 AGGCCTACAG AGAAGCAGTA TGAGACCCTG GAGAACCAGC TGGCATTCCT GTGCCAACAA
     2821 GGCTTCTCCC TGGAAAATGC CTTGTATGCC CTCTCTGCTG TGGGCCACTT CACCTTGGGC
     2881 TGTGTGCTGG AGGACCAGGA GCACCAAGTT GCCAAGGAGG AGAGGGAGAC CCCCACCACT
     2941 GACTCCATGC CACCACTGCT GCGGCAAGCT ATTGAGTTGT TTGACCACCA AGGGGCTGAG
     3001 CCTGCATTCC TTTTTGGCCT GGAACTGATC ATCTGTGGCC TGGAAAAGCA GCTGAAATGT
     3061 GAGTCTGGCT CTCCTAAGAA AAAGAGGAAG GTGCACGGAG GAGGTGGAAG CGGAGGAGGA
     3121 GGAAGCGGAG GAGGAGGTAG CcgATTAATA ATACGACTCA CTATAGGGgc caccATGGAC
     3181 TACAAGGATG ACGACGATAA AAAGCTTCAG CAAGAGGAAG AAGACCGACA GTTGGCTCTG
     3241 CAGTTGCAGC GCATGTTCGA CAATGAGAGG CGGACTGTGA GCCGGCGAAA AGGAAGTGTG
     3301 GATCAGTATC TCCTACGGTC CAGCAACATG GCCGGGGCCA AGTAGACGCG TAAATGATTG
     3361 CAGATCCACT AGTTCTAGAG CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC
     3421 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT
     3481 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT
     3541 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC
     3601 TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCGAGATC
     3661 CACTAGTTCT AGCCTCGAGG CTAGAGCggc cCCGGTGCCT AGAGAAGGTG GCGCGGGGTA
     3721 AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG
     3781 TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA
     3841 CAGGTAAGTG CCGTGTGTGG TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC
     3901 GTGCCTTGAA TTACTTCCAC CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT
     3961 GGAAGTGGGT GGGAGAGTTC GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA
     4021 GTTGAGGCCT GGCCTGGGCG CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC
     4081 TGTCTCGCTG CTTTCGATAA GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG
     4141 CTTTTTTTCT GGCAAGATAG TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG
     4201 GTTTTTGGGG CCGCGGGCGG CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG
     4261 CGGGGCCTGC GAGCGCGGCC ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT
     4321 GCTCTGGTGC CTGGCCTCGC GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC
     4381 CGGTCGGCAC CAGTTGCGTG AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC
     4441 TCAAAATGGA GGACGCGGCG CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA
     4501 AGGGCCTTTC CGTCCTCAGC CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC
     4561 AGGCACCTCG ATTAGTTCTC GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG
     4621 TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG
     4681 CACTTGATGT AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC
     4741 AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG Agcgatcgca
     4801 tcgacagtGT TTAAACgcca ccATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT
     4861 GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGAGGGGCGA
     4921 GGGCGAGGGC GATGCCACCA ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA
     4981 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGAGC CACGGCGTGC AGTGCTTCGC
     5041 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA
     5101 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCACC TACAAGACCC GCGCCGAGGT
     5161 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCGTCG ACTTCAAGGA
     5221 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTTC AACAGCCACA ACATCTATAT
     5281 CATGGCCGTC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACGTGGA
     5341 GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC
     5401 CGTGCTGCTG CCCGACAGCC ACTACCTGAG CACCCAGTCC GTGCTGAGCA AAGACCCCAA
     5461 CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCCGCACC GCCGCCGGGA TCACTCTCGG
     5521 CATGGACGAG CTGTACAAGT GAGAATTCCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT
     5581 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT
     5641 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG
     5701 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAG
     5761 AATAGCAGGC ATGCgtttaa acattaGTCG ACAGGGCCGC CACTGGCCGT CGTTTTACAA
     5821 CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT
     5881 TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC
     5941 AGCCTGAATG GCGAATGGGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG
     6001 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC
     6061 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC
     6121 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT
     6181 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG
     6241 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG
     6301 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG
     6361 CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGCTTAC AATTTAGGTG
     6421 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
     6481 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
     6541 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
     6601 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
     6661 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
     6721 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
     6781 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG
     6841 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG
     6901 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA
     6961 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC
     7021 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA
     7081 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC
     7141 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC
     7201 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG
     7261 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA
     7321 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG
     7381 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA
     7441 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC
     7501 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
     7561 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
     7621 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
     7681 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT
     7741 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
     7801 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
     7861 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
     7921 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
     7981 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
     8041 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
     8101 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
     8161 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
     8221 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
     8281 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
     8341 CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA
     8401 GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA
     8461 GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT
     8521 GTGGAATTGT GAGCGGATAA CAATTTCAC
//
