LOCUS       Exported                6440 bp ds-DNA     circular SYN 05-OCT-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Eric Schreiter
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    57..223
                     /label=ITR
                     /note="ITR"
     misc_feature    246..718
                     /label=synapsin promoter
                     /note="synapsin promoter"
     misc_feature    593..613
                     /label=f.hsyn.347_for.03.76 (Primer box 4)
                     /note="f.hsyn.347_for.03.76 (Primer box 4)"
     primer_bind     707..737
                     /label=F-loxP
     misc_feature    744..777
                     /label=loxP2272
                     /note="loxP2272"
     misc_feature    840..873
                     /label=loxP
                     /note="loxP"
     CDS             complement(884..2737)
                     /codon_start=1
                     /label=VOLTRON_SOM2
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYINWVLTTPLLLLDLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFKIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAP
                     THRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWA
                     KRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPM
                     GVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPV
                     PKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTL
                     EISGEPTTKSRITSEGEYIPLDQIDINVFCYENEVQSQPILNTKEMAPQSKPPEELEMS
                     SMPSPVAPLPARTEGVIDMRSMSSIDSFISCATDFPEATRF"
     CDS             complement(887..1081)
                     /codon_start=1
                     /label=Kir2.1_proximal restriction and clustering signal
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRF"
     CDS             complement(1082..1102)
                     /codon_start=1
                     /label=Kir2.1_ER_export_signal
                     /translation="FCYENEV"
     CDS             complement(1103..1162)
                     /codon_start=1
                     /label=Kir2.1_membrane_trafficking_signal
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             complement(1163..2056)
                     /codon_start=1
                     /label=HaloTag
                     /translation="IGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVW
                     RNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDW
                     GSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQN
                     VFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEY
                     MDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGS
                     EIARWLSTLEISGEPTT"
     CDS             complement(2057..2737)
                     /codon_start=1
                     /label=Ace2N
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYINWVLTTPLLLLDLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFK"
     primer_bind     complement(2719..2780)
                     /label=R-Ace2N-G-synflex
     misc_feature    complement(2754..2787)
                     /label=loxp2272
                     /note="loxp2272"
     misc_feature    complement(2858..2891)
                     /label=loxP
                     /note="loxP"
     misc_feature    2933..3521
                     /label=WPRE
                     /note="WPRE"
     misc_feature    3004..3023
                     /label=r.WPRE seq rev.03.15 (Primer box 1)
                     /note="r.WPRE seq rev.03.15 (Primer box 1)"
     misc_feature    3555..3786
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3815..3981
                     /label=ITR
                     /note="ITR"
     misc_feature    4640..5497
                     /label=Amp
                     /note="Amp"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatctctgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg
      301 ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt
      361 ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg
      421 tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc
      481 caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc
      541 cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg
      601 aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg
      661 ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaat
      721 tcaagctgct agcaaggatc cccataactt cgtataaagt atcctatacg aagttatatc
      781 aaaataggaa gaccaatgct tcaccatcga cccgaattgc caagcatcac catcgaccca
      841 taacttcgta taatgtatgc tatacgaagt tatactagag cttttagaat cgagtagcct
      901 cggggaaatc tgttgcgcaa gagatgaagc tatcaataga tgacatactc ctcatgtcaa
      961 tcactccttc agtcctggca gggaggggag ccacgggact tggcatactg gacatctcaa
     1021 gctcttcggg aggcttagac tgtggagcca tttcctttgt gttcaggata ggctgacttt
     1081 gcacctcgtt ctcgtagcag aacacgttga tgtcgatctg gtccaggggg atgtactcgc
     1141 cctcgctggt gatcctgctc ttagtggttg gctcgccgga aatctcgagc gtcgacagcc
     1201 agcgcgcgat ctcgctgccg atcaggtccg ggttgtcttc ttgcagcaga ttcagacccg
     1261 ggccgatgtc cacagccttg cagttaggca ggcttttggc caggcgagcg gcttcggccg
     1321 gtgggatcag aacgcctggg gtgccccaga acagcagctt cgggacaggg gactggtgca
     1381 gccagtccat gtattcttcg accagcgcga cgatgttcgc tggctcaccg gcgattggca
     1441 gctcgtttgg gaagcgccac agtggctcgc ggtcaacagg attcaggaac ggctcgcggt
     1501 aatggtccat ctcgacttca gtcagcgggc ggacgacacc catcggcagc gtaccctcga
     1561 taaaaacgtt ctgatcgatg atcagcttgc ggccgacgtc ggtggtgcgg aaggcctgga
     1621 aggtctcgcg ggcaaattct ggccattcgt cccaggtcgg gatagggcgg atgaactcca
     1681 taaatgcaat acctttgacg cgctctggat tgcgcttggc ccagtggaaa cccagagcgg
     1741 agccccagtc gtgaatgacc aggacgacct cttccagacc cagggcttcg atgaaggcat
     1801 ccatgaagcg gacgtggtcg tcgaagaaat aacccaggtc tggtttgtcg gatttgccca
     1861 taccgatcag gtctggagca atgcagcgat gggtcggtgc aacatgcggg atgatgttgc
     1921 gccacacgta ggaggaggtc gggttaccgt gcaggaacag cacaggggtg ccatcgcgcg
     1981 gaccaacatc gacgtagtgc atgcgctcgc ccaggacttc cacataatgg gggtcgaatg
     2041 gaaagccagt accgatcttg aagatagtct catgggcaat gaggcacccc attccaaaca
     2101 gtggcttagc gagcacatcc aggattccca tgagaatggc ctcattgtcg acggacagca
     2161 cgccactacc agacccgaaa gcccacacga caggataaaa cacccagagg attgacagga
     2221 agacgagcag actgacatag gagctatggt actcggggct tttcttcaga tcgtctttcc
     2281 aagtggcgtt atacatcccg tatgtcagga ctgcctgcat cacacatcca gcgataaagt
     2341 acacccattt gaacttgtgt tcatcctcga aggcgcccag aataccaaac acgatcatga
     2401 aaatgtctgc gccgatgacc caagaaatca tcactccgcc catcttggtc atgacgatga
     2461 gatcgagcag gagcagtggt gtggtcagca cccagttaat atatcttgcg tagaccacct
     2521 gcctttcacc attcattgtc aggtcctggt agttcacggc cattgcatag taagcggtag
     2581 cggcgatagt gcagatggca atgttgatat agtatgcgct cttcagctct gaaggccgaa
     2641 agtgcacagc cactccaaaa gcaatagcag cagcagccat aatagcaaag acaatccact
     2701 gtgcaatcat gccggtctcg gtttccacgt cagccatggt ggcggatcct agtataactt
     2761 cgtataggat actttatacg aagttatcat tgggattctt cctattttga tccaagcatc
     2821 accatcgacc ctctagtcca gatctcacca tcgacccata acttcgtata gcatacatta
     2881 tacgaagtta tgtccctcga agaggttcga attcgatatc aagcttatcg ataatcaacc
     2941 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3001 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3061 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3121 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3181 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3241 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3301 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     3361 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3421 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3481 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     3541 acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac
     3601 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3661 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     3721 tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     3781 taaaatcgat aaggatcttc ctagagcatg gctacgtaga taagtagcat ggcgggttaa
     3841 tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     3901 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     3961 cagtgagcga gcgagcgcgc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4021 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     4081 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4141 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4201 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4261 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4321 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4381 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4441 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     4501 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4561 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4621 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4681 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4741 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4801 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4861 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4921 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4981 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5041 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5101 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5161 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5221 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5281 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5341 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5401 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5461 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5521 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5581 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6061 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6301 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6361 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6421 agtgagcgag gaagcggaag
//
