LOCUS       Exported                8987 bp ds-DNA     circular SYN 08-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 18, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..352
                     /locus_tag="CMV Continued"
                     /label=CMV Continued
     CDS             393..1550
                     /locus_tag="A3DE"
                     /label=A3DE
     misc_feature    1551..1598
                     /locus_tag="XTEN Linker"
                     /label=XTEN Linker
     misc_feature    1599..5699
                     /locus_tag="Cas9"
                     /label=Cas9
     misc_feature    5712..5960
                     /locus_tag="UGI"
                     /label=UGI
     misc_feature    5973..5993
                     /locus_tag="SV40 NLS"
                     /label=SV40 NLS
     misc_feature    8765..8987
                     /locus_tag="CMV Start"
                     /label=CMV Start
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgcgccg ccaccatgat ggatgaatcc acagatcaga aatccgatgg
      421 agcggatgta tcgagacaca ttctacgaca actttgaaaa cgaacccatc ctctatggtc
      481 ggagctacac ttggctgtgc tatgaagtga aaataaagag gggccgctca aatctccttt
      541 gggacacagg ggtctttcga ggcccggtac tacccaaacg tcagtcgaat cacaggcagg
      601 aggtgtattt ccggtttgag aaccacgcag aaatgtgctt cttatcttgg ttctgtggca
      661 accgactgcc tgctaacagg cgcttccaga tcacctggtt tgtatcatgg aacccctgcc
      721 tgccctgtgt ggtgaaggtg accaaattct tggctgagca ccccaatgtc accctgacca
      781 tctctgccgc ccgcctctac tactaccggg atagagattg gcggtgggtg ctcctcaggc
      841 tgcataaggc aggggcccgt gtgaagatca tggactatga agactttgca tactgctggg
      901 aaaactttgt gtgcaatgaa ggtcagccat tcatgccttg gtacaaattc gatgacaatt
      961 atgcatccct gcaccgcacg ctaaaggaga ttctcagaaa cccgatggag gcaatgtacc
     1021 cacacatatt ctacttccac tttaaaaacc tactgaaagc ctgtggtcgg aacgaaagct
     1081 ggctgtgctt caccatggaa gttacaaagc accactcagc tgtcttccgg aagaggggcg
     1141 tcttccgaaa ccaggtggat cctgagaccc attgtcatgc agaaaggtgc ttcctctctt
     1201 ggttctgtga cgacatactg tctcctaaca caaactacga ggtcacctgg tacacatctt
     1261 ggagcccttg cccagagtgt gcaggggagg tggccgagtt cctggccagg cacagcaacg
     1321 tgaatctcac catcttcacc gcccgcctct gctatttctg ggatacagat taccaggagg
     1381 ggctctgcag cctgagtcag gaaggggcct ccgtgaagat catgggctac aaagattttg
     1441 tatcttgttg gaaaaacttt gtgtacagtg atgatgagcc attcaagcct tggaagggac
     1501 tacaaaccaa ctttcgactt ctgaaaagaa ggctacggga gattctccag agcggcagcg
     1561 agactcccgg gacctcagag tccgccacac ccgaaagtga taaaaagtat tctattggtt
     1621 tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac aaagtacctt
     1681 caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag aatcttatcg
     1741 gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa cgaaccgctc
     1801 ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt tttagcaatg
     1861 agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc cttgtcgaag
     1921 aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag gtggcatatc
     1981 atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca actgataaag
     2041 cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt gggcactttc
     2101 tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc atccagttag
     2161 tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc gtggatgcga
     2221 aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg atcgcacaat
     2281 tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca ctaggcctga
     2341 caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag cttagtaagg
     2401 acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag tatgcggact
     2461 tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata ctgagagtta
     2521 atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac gatgaacatc
     2581 accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag aaatataagg
     2641 aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc ggagcgagtc
     2701 aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg acggaagagt
     2761 tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc gacaacggta
     2821 gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg caggaggatt
     2881 tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc tttcgcatac
     2941 cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg acaagaaagt
     3001 ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt gcgtcagctc
     3061 aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa aaagtattgc
     3121 ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg aaagttaagt
     3181 atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag aaagcaatag
     3241 tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa gaggactact
     3301 ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat cgatttaatg
     3361 cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac ttcctggata
     3421 acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc tttgaagatc
     3481 gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat aaggttatga
     3541 aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa cttatcaacg
     3601 ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc gacggcttcg
     3661 ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa gaggatatac
     3721 aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg aatcttgctg
     3781 gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat gagctagtta
     3841 aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc gaaaatcaaa
     3901 cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa gagggtatta
     3961 aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa ttgcagaacg
     4021 agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat caggaactgg
     4081 acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc tttttgaagg
     4141 acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg aaaagtgaca
     4201 atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag ctcctaaatg
     4261 cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg ggtggcttgt
     4321 ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc caaatcacaa
     4381 agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag aacgataagc
     4441 tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac ttcagaaagg
     4501 attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac gacgcttatc
     4561 ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa agtgagtttg
     4621 tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc gaacaggaga
     4681 taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc tttaagacgg
     4741 aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc aatggggaga
     4801 caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa gttttgtcca
     4861 tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt tcaaaggaat
     4921 cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac tgggacccga
     4981 aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta gtggcaaaag
     5041 ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg ataacgatta
     5101 tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa ggttacaagg
     5161 aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag ttagaaaatg
     5221 gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa ctcgcactac
     5281 cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg aaaggttcac
     5341 ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat ctcgacgaaa
     5401 tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc aatctggaca
     5461 aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag gcggaaaata
     5521 ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag tattttgaca
     5581 caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg acactgattc
     5641 accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt gggggtgact
     5701 ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag caactggtta
     5761 tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg aacaagccgg
     5821 aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat gtcatgcttc
     5881 tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat agcaacggtg
     5941 agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa gtctaaccgg
     6001 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag
     6061 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6121 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6181 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6241 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6301 ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6361 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6421 agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6481 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6541 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6601 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6841 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6901 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6961 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7021 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7081 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7141 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7201 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7261 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7321 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7381 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7441 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7501 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7561 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7621 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7681 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7741 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7801 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7861 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7921 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7981 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8041 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8101 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8161 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8221 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8281 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8341 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8401 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8461 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc
     8521 tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca
     8581 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     8641 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     8701 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     8761 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     8821 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     8881 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     8941 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc
//
