LOCUS       Exported                9279 bp ds-DNA     circular SYN 28-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 18, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..352
                     /label=CMV Continued
     misc_feature    389..538
                     /label=A3HExon1
     misc_feature    389..538
                     /label=A3H (II)
                     /label=A3H (II)(1)
     misc_feature    539..1440
                     /label=b-globin intron
     misc_feature    join(1441..1811,1818..1842)
                     /label=A3H (II)
     misc_feature    1441..1708
                     /label=A3H Exon2
     misc_feature    1843..1890
                     /label=XTEN Linker
     misc_feature    1891..5991
                     /label=Cas9
     misc_feature    6004..6252
                     /label=UGI
     misc_feature    6265..6285
                     /label=SV40 NLS
     misc_feature    9057..9279
                     /label=CMV Start
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgcgccg ccaccatgat ggctctgtta acagccgaaa cattccgctt
      421 acagtttaac aacaagcgcc gcctcagaag gccttactac ccgaggaagg ccctcttgtg
      481 ttaccagctg acgccgcaga atggctccac gcccacgaga ggctactttg aaaacaaggt
      541 gagtccagga gatgtttcag cactgttgcc tttagtctcg aggcaactta gacaactgag
      601 tattgatctg agcacagcag ggtgtgagct gtttgaagat actggggttg ggggtgaaga
      661 aactgcagag gactaactgg gctgagaccc agtggcaatg ttttagggcc taaggaatgc
      721 ctctgaaaat ctagatggac aactttgact ttgagaaaag agaggtggaa atgaggaaaa
      781 tgacttttct ttattagatt tcggtagaaa gaactttcat ctttccccta tttttgttat
      841 tcgttttaaa acatctatct ggaggcagga caagtatggt cattaaaaag atgcaggcag
      901 aaggcatata ttggctcagt caaagtgggg aactttggtg gccaaacata cattgctaag
      961 gctattccta tatcagctgg acacatataa aatgctgcta atgcttcatt acaaacttat
     1021 atcctttaat tccagatggg ggcaaagtat gtccaggggt gaggaacaat tgaaacattt
     1081 gggctggagt agattttgaa agtcagctct gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     1141 gtgtgcgcgc acgtgtgttt gtgtgtgtgt gagagcgtgt gtttctttta acgttttcag
     1201 cctacagcat acagggttca tggtggcaag aagataacaa gatttaaatt atggccagtg
     1261 actagtgctg caagaagaac aactacctgc atttaatggg aaagcaaaat ctcaggcttt
     1321 gagggaagtt aacataggct tgattctggg tggaagctgg gtgtgtagtt atctggaggc
     1381 caggctggag ctctcagctc actatgggtt catctttatt gtctcctttc atctcaacag
     1441 aaaaagtgcc atgcggaaat ttgctttatt aacgagatca agtccatggg actggacgaa
     1501 acgcagtgct accaagtcac ctgttacctc acgtggagcc cctgctcctc ctgtgcctgg
     1561 gagctggttg acttcatcaa ggctcacgac catctgaacc tgcgcatctt cgcctcccgc
     1621 ctgtactacc actggtgcaa gccccagcag gacgggctgc ggcttctgtg tggatcccag
     1681 gtcccggtgg aggtcatggg cttcccagag tttgctgact gctgggaaaa ctttgtggac
     1741 cacgagaaac cgctttcctt caacccctat aagatgttag aggagctaga taaaaacagt
     1801 cgagccataa aggaggagct tgacaggata aagcagtcct taagcggcag cgagactccc
     1861 gggacctcag agtccgccac acccgaaagt gataaaaagt attctattgg tttagccatc
     1921 ggcactaatt ccgttggatg ggctgtcata accgatgaat acaaagtacc ttcaaagaaa
     1981 tttaaggtgt tggggaacac agaccgtcat tcgattaaaa agaatcttat cggtgccctc
     2041 ctattcgata gtggcgaaac ggcagaggcg actcgcctga aacgaaccgc tcggagaagg
     2101 tatacacgtc gcaagaaccg aatatgttac ttacaagaaa tttttagcaa tgagatggcc
     2161 aaagttgacg attctttctt tcaccgtttg gaagagtcct tccttgtcga agaggacaag
     2221 aaacatgaac ggcaccccat ctttggaaac atagtagatg aggtggcata tcatgaaaag
     2281 tacccaacga tttatcacct cagaaaaaag ctagttgact caactgataa agcggacctg
     2341 aggttaatct acttggctct tgcccatatg ataaagttcc gtgggcactt tctcattgag
     2401 ggtgatctaa atccggacaa ctcggatgtc gacaaactgt tcatccagtt agtacaaacc
     2461 tataatcagt tgtttgaaga gaaccctata aatgcaagtg gcgtggatgc gaaggctatt
     2521 cttagcgccc gcctctctaa atcccgacgg ctagaaaacc tgatcgcaca attacccgga
     2581 gagaagaaaa atgggttgtt cggtaacctt atagcgctct cactaggcct gacaccaaat
     2641 tttaagtcga acttcgactt agctgaagat gccaaattgc agcttagtaa ggacacgtac
     2701 gatgacgatc tcgacaatct actggcacaa attggagatc agtatgcgga cttatttttg
     2761 gctgccaaaa accttagcga tgcaatcctc ctatctgaca tactgagagt taatactgag
     2821 attaccaagg cgccgttatc cgcttcaatg atcaaaaggt acgatgaaca tcaccaagac
     2881 ttgacacttc tcaaggccct agtccgtcag caactgcctg agaaatataa ggaaatattc
     2941 tttgatcagt cgaaaaacgg gtacgcaggt tatattgacg gcggagcgag tcaagaggaa
     3001 ttctacaagt ttatcaaacc catattagag aagatggatg ggacggaaga gttgcttgta
     3061 aaactcaatc gcgaagatct actgcgaaag cagcggactt tcgacaacgg tagcattcca
     3121 catcaaatcc acttaggcga attgcatgct atacttagaa ggcaggagga tttttatccg
     3181 ttcctcaaag acaatcgtga aaagattgag aaaatcctaa cctttcgcat accttactat
     3241 gtgggacccc tggcccgagg gaactctcgg ttcgcatgga tgacaagaaa gtccgaagaa
     3301 acgattactc catggaattt tgaggaagtt gtcgataaag gtgcgtcagc tcaatcgttc
     3361 atcgagagga tgaccaactt tgacaagaat ttaccgaacg aaaaagtatt gcctaagcac
     3421 agtttacttt acgagtattt cacagtgtac aatgaactca cgaaagttaa gtatgtcact
     3481 gagggcatgc gtaaacccgc ctttctaagc ggagaacaga agaaagcaat agtagatctg
     3541 ttattcaaga ccaaccgcaa agtgacagtt aagcaattga aagaggacta ctttaagaaa
     3601 attgaatgct tcgattctgt cgagatctcc ggggtagaag atcgatttaa tgcgtcactt
     3661 ggtacgtatc atgacctcct aaagataatt aaagataagg acttcctgga taacgaagag
     3721 aatgaagata tcttagaaga tatagtgttg actcttaccc tctttgaaga tcgggaaatg
     3781 attgaggaaa gactaaaaac atacgctcac ctgttcgacg ataaggttat gaaacagtta
     3841 aagaggcgtc gctatacggg ctggggacga ttgtcgcgga aacttatcaa cgggataaga
     3901 gacaagcaaa gtggtaaaac tattctcgat tttctaaaga gcgacggctt cgccaatagg
     3961 aactttatgc agctgatcca tgatgactct ttaaccttca aagaggatat acaaaaggca
     4021 caggtttccg gacaagggga ctcattgcac gaacatattg cgaatcttgc tggttcgcca
     4081 gccatcaaaa agggcatact ccagacagtc aaagtagtgg atgagctagt taaggtcatg
     4141 ggacgtcaca aaccggaaaa cattgtaatc gagatggcac gcgaaaatca aacgactcag
     4201 aaggggcaaa aaaacagtcg agagcggatg aagagaatag aagagggtat taaagaactg
     4261 ggcagccaga tcttaaagga gcatcctgtg gaaaataccc aattgcagaa cgagaaactt
     4321 tacctctatt acctacaaaa tggaagggac atgtatgttg atcaggaact ggacataaac
     4381 cgtttatctg attacgacgt cgatcacatt gtaccccaat cctttttgaa ggacgattca
     4441 atcgacaata aagtgcttac acgctcggat aagaaccgag ggaaaagtga caatgttcca
     4501 agcgaggaag tcgtaaagaa aatgaagaac tattggcggc agctcctaaa tgcgaaactg
     4561 ataacgcaaa gaaagttcga taacttaact aaagctgaga ggggtggctt gtctgaactt
     4621 gacaaggccg gatttattaa acgtcagctc gtggaaaccc gccaaatcac aaagcatgtt
     4681 gcacagatac tagattcccg aatgaatacg aaatacgacg agaacgataa gctgattcgg
     4741 gaagtcaaag taatcacttt aaagtcaaaa ttggtgtcgg acttcagaaa ggattttcaa
     4801 ttctataaag ttagggagat aaataactac caccatgcgc acgacgctta tcttaatgcc
     4861 gtcgtaggga ccgcactcat taagaaatac ccgaagctag aaagtgagtt tgtgtatggt
     4921 gattacaaag tttatgacgt ccgtaagatg atcgcgaaaa gcgaacagga gataggcaag
     4981 gctacagcca aatacttctt ttattctaac attatgaatt tctttaagac ggaaatcact
     5041 ctggcaaacg gagagatacg caaacgacct ttaattgaaa ccaatgggga gacaggtgaa
     5101 atcgtatggg ataagggccg ggacttcgcg acggtgagaa aagttttgtc catgccccaa
     5161 gtcaacatag taaagaaaac tgaggtgcag accggagggt tttcaaagga atcgattctt
     5221 ccaaaaagga atagtgataa gctcatcgct cgtaaaaagg actgggaccc gaaaaagtac
     5281 ggtggcttcg atagccctac agttgcctat tctgtcctag tagtggcaaa agttgagaag
     5341 ggaaaatcca agaaactgaa gtcagtcaaa gaattattgg ggataacgat tatggagcgc
     5401 tcgtcttttg aaaagaaccc catcgacttc cttgaggcga aaggttacaa ggaagtaaaa
     5461 aaggatctca taattaaact accaaagtat agtctgtttg agttagaaaa tggccgaaaa
     5521 cggatgttgg ctagcgccgg agagcttcaa aaggggaacg aactcgcact accgtctaaa
     5581 tacgtgaatt tcctgtattt agcgtcccat tacgagaagt tgaaaggttc acctgaagat
     5641 aacgaacaga agcaactttt tgttgagcag cacaaacatt atctcgacga aatcatagag
     5701 caaatttcgg aattcagtaa gagagtcatc ctagctgatg ccaatctgga caaagtatta
     5761 agcgcataca acaagcacag ggataaaccc atacgtgagc aggcggaaaa tattatccat
     5821 ttgtttactc ttaccaacct cggcgctcca gccgcattca agtattttga cacaacgata
     5881 gatcgcaaac gatacacttc taccaaggag gtgctagacg cgacactgat tcaccaatcc
     5941 atcacgggat tatatgaaac tcggatagat ttgtcacagc ttgggggtga ctctggtggt
     6001 tctactaatc tgtcagatat tattgaaaag gagaccggta agcaactggt tatccaggaa
     6061 tccatcctca tgctcccaga ggaggtggaa gaagtcattg ggaacaagcc ggaaagcgat
     6121 atactcgtgc acaccgccta cgacgagagc accgacgaga atgtcatgct tctgactagc
     6181 gacgcccctg aatacaagcc ttgggctctg gtcatacagg atagcaacgg tgagaacaag
     6241 attaagatgc tctctggtgg ttctcccaag aagaagagga aagtctaacc ggtcatcatc
     6301 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     6361 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6421 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6481 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6541 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     6601 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6661 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     6721 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6781 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6841 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6961 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7141 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7201 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7441 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7501 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7561 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7621 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7681 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7741 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7801 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7861 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7921 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7981 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8041 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8101 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8161 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8221 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8281 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8341 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8401 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8461 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8521 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8581 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8641 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8701 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8761 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
     8821 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     8881 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     8941 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     9001 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     9061 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     9121 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     9181 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     9241 tacggtaaac tgcccacttg gcagtacatc aagtgtatc
//
