LOCUS       pCSM166_-_sgRNA-anti-P7 2183 bp ds-DNA     circular     18-JUN-2018
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     misc_feature    484..518
                     /label="promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    770..788
                     /label="mark"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    622..734
                     /label="terminator"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(1868..448)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    545..546
                     /label="bulge"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(879..1736)
                     /label="AmpR"
                     /ApEinfo_revcolor=#9f4240
                     /ApEinfo_fwdcolor=#9f4240
     misc_feature    587..598
                     /label="hairpin1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    555..621
                     /label="tracr"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    437..454
                     /label="mark"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(1737..1867)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    569..581
                     /label="nexus"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    519..538
                     /label="M2S"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    539..550
                     /label="repeat"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    600..614
                     /label="hairpin2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    539..544
                     /label="lower_stem"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     protein_bind    770..773
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    559..562
                     /label="bulge"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    547..550
                     /label="upper_stem"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    563..568
                     /label="lower_stem"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    555..558
                     /label="upper_stem"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      complement(782..878)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
       61 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      421 gttatcccct gattctgtgg ataaccgtgc ggccCGAGAG CATCGATCCT AGCATGGatg
      481 catTTGACAG CTAGCTCAGT CCTAGGTATA ATGCTAGCGG TAAAATTGTC AACACGGTGT
      541 TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG
      601 CACCGAGTCG GTGCTTTTTT Tccggcttat cggtcagttt cacctgattt acgtaaaaac
      661 ccgcttcggc gggtttttgc ttttggaggg gcagaaagat gaatgactgt ccacgacgct
      721 atacccaaaa gaaaaagctt GAAAGAATCG ATCGATCCCG AATGAGTAGt acagcggccg
      781 cgattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      841 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      901 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      961 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgggac
     1021 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1081 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1141 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1201 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1261 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1321 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1381 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1441 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1501 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1561 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1621 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1681 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1741 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1801 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1861 ccacctgtca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2161 ctaatcctgt taccagtggc tgc
//