LOCUS Exported 16228 bp ds-DNA circular SYN 19-OCT-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 16228) AUTHORS Stanford University TITLE Direct Submission JOURNAL Exported Oct 19, 2018 from SnapGene 4.2.6 http://www.snapgene.com FEATURES Location/Qualifiers source 1..16228 /organism="recombinant plasmid" /mol_type="other DNA" promoter 295..607 /label=tight TRE promoter /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" protein_bind 303..321 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 339..357 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 374..392 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 410..428 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 446..464 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 481..499 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 517..535 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" CDS 1018..6852 /codon_start=1 /label=DPI (1-1945) /translation="MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRR GVITDQNSDGYCQTGTMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQLTRS RELDECFAQANDQMEILDSLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRR ASSKGGGGYTCQSGSGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHR GIHNSIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSR EIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQ HPASDKIEAYMDTLQTQWSWILQITKCIDVHLKENAAYFQFFEEAQSTEAYLKGLQDSI RKKYPCDKNMPLQHLLEQIKELEKEREKILEYKRQVQNLVNKSKKIVQLKPRNPDYRSN KPIILRALCDYKQDQKIVHKGDECILKDNNERSKWYVTGPGGVDMLVPSVGLIIPPPNP LAVDLSCKIEQYYEAILALWNQLYINMKSLVSWHYCMIDIEKIRAMTIAKLKTMRQEDY MKTIADLELHYQEFIRNSQGSEMFGDDDKRKIQSQFTDAQKHYQTLVIQLPGYPQHQTV TTTEITHHGTCQDVNHNKVIETNRENDKQETWMLMELQKIRRQIEHCEGRMTLKNLPLA DQGSSHHITVKINELKSVQNDSQAIAEVLNQLKDMLANFRGSEKYCYLQNEVFGLFQKL ENINGVTDGYLNSLCTVRALLQAILQTEDMLKVYEARLTEEETVCLDLDKVEAYRCGLK KIKNDLNLKKSLLATMKTELQKAQQIHSQTSQQYPLYDLDLGKFGEKVTQLTDRWQRID KQIDFRLWDLEKQIKQLRNYRDNYQAFCKWLYDAKRRQDSLESMKFGDSNTVMRFLNEQ KNLHSEISGKRDKSEEVQKIAELCANSIKDYELQLASYTSGLETLLNIPIKRTMIQSPS GVILQEAADVHARYIELLTRSGDYYRFLSEMLKSLEDLKLKNTKIEVLEEELRLARDAN SENCNKNKFLDQNLQKYQAECSQFKAKLASLEELKRQAELDGKSAKQNLDKCYGQIKEL NEKITRLTYEIEDEKRRRKSVEDRFDQQKNDYDQLQKARQCEKENLGWQKLESEKAIKE KEYEIERLRVLLQEEGTRKREYENELAKVRNHYNEEMSNLRNKYETEINITKTTIKEIS MQKEDDSKNLRNQLDRLSRENRDLKDEIVRLNDSILQATEQRRRAEENALQQKACGSEI MQKKQHLEIELKQVMQQRSEDNARHKQSLEEAAKTIQDKNKEIERLKAEFQEEAKRRWE YENELSKVRNNYDEEIISLKNQFETEINITKTTIHQLTMQKEEDTSGYRAQIDNLTREN RSLSEEIKRLKNTLTQTTENLRRVEEDIQQQKATGSEVSQRKQQLEVELRQVTQMRTEE SVRYKQSLDDAAKTIQDKNKEIERLKQLIDKETNDRKCLEDENARLQRVQYDLQKANSS ATETINKLKVQEQELTRLRIDYERVSQERTVKDQDITRFQNSLKELQLQKQKVEEELNR LKRTASEDSCKRKKLEEELEGMRRSLKEQAIKITNLTQQLEQASIVKKRSEDDLRQQRD VLDGHLREKQRTQEELRRLSSEVEALRRQLLQEQESVKQAHLRNEHFQKAIEDKSRSLN ESKIEIERLQSLTENLTKEHLMLEEELRNLRLEYDDLRRGRSEADSDKNATILELRSQL QISNNRTLELQGLINDLQRERENLRQEIEKFQKQALEASNRIQESKNQCTQVVQERESL LVKIKVLEQDKARLQRLEDELNRAKSTLEAETRVKQRLECEKQQIQNDLNQWKTQYSRK EEAIRKIESEREKSEREKNSLRSEIERLQAEIKRIEERCRRKLEDSTRETQSQLETERS RYQREIDKLRQR" CDS 6862..6921 /codon_start=1 /label=20AA Linker /translation="GSGGTGSTSGGSGGSTGGGT" CDS 6928..7632 /codon_start=1 /label=mTFP1 /translation="VSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDGT NTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTFE DKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLKG DVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESAV ARNSTDGMDELYK" CDS 7633..7752 /codon_start=1 /label=Flagelliform /translation="GPGGAGPGGAGPGGAGPGGAGPGGAGPGGAGPGGAGPGGA" CDS 7753..8469 /codon_start=1 /label=mEYFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 8470..8532 /codon_start=1 /label=21AA Linker /translation="GTGGGTSGGSGGSTSGTGGSG" CDS 8542..11322 /codon_start=1 /label=DPI (1946-End) /translation="PYGSHRETQTECEWTVDTSKLVFDGLRKKVTAMQLYECQLIDKTT LDKLLKGKKSVEEVASEIQPFLRGAGSIAGASASPKEKYSLVEAKRKKLISPESTVMLL EAQAATGGIIDPHRNEKLTVDSAIARDLIDFDDRQQIYAAEKAITGFDDPFSGKTVSVS EAIKKNLIDRETGMRLLEAQIASGGVVDPVNSVFLPKDVALARGLIDRDLYRSLNDPRD SQKNFVDPVTKKKVSYVQLKERCRIEPHTGLLLLSVQKRSMSFQGIRQPVTVTELVDSG ILRPSTVNELESGQISYDEVGERIKDFLQGSSCIAGIYNETTKQKLGIYEAMKIGLVRP GTALELLEAQAATGFIVDPVSNLRLPVEEAYKRGLVGIEFKEKLLSAERAVTGYNDPET GNIISLFQAMNKELIEKGHGIRLLEAQIATGGIIDPKESHRLPVDIAYKRGYFNEELSE ILSDPSDDTKGFFDPNTEENLTYLQLKERCIKDEETGLCLLPLKEKKKQVQTSQKNTLR KRRVVIVDPETNKEMSVQEAYKKGLIDYETFKELCEQECEWEEITITGSDGSTRVVLVD RKTGSQYDIQDAIDKGLVDRKFFDQYRSGSLSLTQFADMISLKNGVGTSSSMGSGVSDD VFSSSRHESVSKISTISSVRNLTIRSSSFSDTLEESSPIAAIFDTENLEKISITEGIER GIVDSITGQRLLEAQACTGGIIHPTTGQKLSLQDAVSQGVIDQDMATRLKPAQKAFIGF EGVKGKKKMSAAEAVKEKWLPYEAGQRFLEFQYLTGGLVDPEVHGRISTEEAIRKGFID GRAAQRLQDTSSYAKILTCPKTKLKISYKDAINRSMVEDITGLRLLEAASVSSKGLPSP YNMSSAPGSRSGSRSGSRSGSRSGSRSGSRRGGFDATGNSSYSYSYSFSSSSIGH" CDS 11251..11253 /label=S2849G Site polyA_signal 11364..11485 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS 12208..12951 /codon_start=1 /product="improved tetracycline-controlled transactivator" /label=rtTA-Advanced /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline." /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" CDS 12958..13014 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 13015..13614 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 13675..13899 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin complement(14396..14984) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(15155..16015) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(16016..16120) /gene="bla" /label=AmpR promoter ORIGIN 1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact 61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta 121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta 181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt 241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agacgagttt 301 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg 361 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt 421 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta 481 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta 541 tgtcgattag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca 601 gatcgcctgg agccaattcc aactcttttg ccttatacct actttccgta ccacttccta 661 ccctcgaaag gcctctgcgg ccgcgtcaac agcccctgct ccttgggccc ctccatgcca 721 tgccgtaatc tctcccaccc gaccaacacc aacacccagc tccgacgcag ctcctctgcg 781 cccttgccgc cctccgagcc acagctttcc tcccgctcct gcccccggcc cgtcgccgtc 841 tccgcgctcg cagcggcctc gggagggccc aggtagcgag cagcgacctc gcgagccttc 901 cgcactcccg cccggttccc cggccgtccg cctatccttg gccccctccg ctttctccgc 961 gccggcccgc ctcgcttatg cctcggcgct gagccgctct cccgattgcc cgccgacatg 1021 agctgcaacg gaggctccca cccgcggatc aacactctgg gccgcatgat ccgcgccgag 1081 tctggcccgg acctgcgcta cgaggtgacc agcggcggcg ggggcaccag caggatgtac 1141 tattctcggc gcggcgtgat caccgaccag aactcggacg gctactgtca aaccggcacg 1201 atgtccaggc accagaacca gaacaccatc caggagctgc tgcagaactg ctccgactgc 1261 ttgatgcgag cagagctcat cgtgcagcct gaattgaagt atggagatgg aatacaactg 1321 actcggagtc gagaattgga tgagtgtttt gcccaggcca atgaccaaat ggaaatcctc 1381 gacagcttga tcagagagat gcggcagatg ggccagccct gtgatgctta ccagaaaagg 1441 cttcttcagc tccaagagca aatgcgagcc ctttataaag ccatcagtgt ccctcgagtc 1501 cgcagggcca gctccaaggg tggtggaggc tacacttgtc agagtggctc tggctgggat 1561 gagttcacca aacatgtcac cagtgaatgt ttggggtgga tgaggcagca aagggcggag 1621 atggacatgg tggcctgggg tgtggacctg gcctcagtgg agcagcacat taacagccac 1681 cggggcatcc acaactccat cggcgactat cgctggcagc tggacaaaat caaagccgac 1741 ctgcgcgaga aatctgcgat ctaccagttg gaggaggagt atgaaaacct gctgaaagcg 1801 tcctttgaga ggatggatca cctgcgacag ctgcagaaca tcattcaggc cacgtccagg 1861 gagatcatgt ggatcaatga ctgcgaggag gaggagctgc tgtacgactg gagcgacaag 1921 aacaccaaca tcgctcagaa acaggaggcc ttctccatac gcatgagtca actggaagtt 1981 aaagaaaaag agctcaataa gctgaaacaa gaaagtgacc aacttgtcct caatcagcat 2041 ccagcttcag acaaaattga ggcctatatg gacactctgc agacgcagtg gagttggatt 2101 cttcagatca ccaagtgcat tgatgttcat ctgaaagaaa atgctgccta ctttcagttt 2161 tttgaagagg cgcagtctac tgaagcatac ctgaaggggc tccaggactc catcaggaag 2221 aagtacccct gcgacaagaa catgcccctg cagcacctgc tggaacagat caaggagctg 2281 gagaaagaac gagagaaaat ccttgaatac aagcgtcagg tgcagaactt ggtaaacaag 2341 tctaagaaga ttgtacagct gaagcctcgt aacccagact acagaagcaa taaacccatt 2401 attctcagag ctctctgtga ctacaaacaa gatcagaaaa tcgtgcataa gggggatgag 2461 tgtatcctga aggacaacaa cgagcgcagc aagtggtacg tgacgggccc gggaggcgtt 2521 gacatgcttg ttccctctgt ggggctgatc atccctcctc cgaacccact ggccgtggac 2581 ctctcttgca agattgagca gtactacgaa gccatcttgg ctctgtggaa ccagctctac 2641 atcaacatga agagcctggt gtcctggcac tactgcatga ttgacataga gaagatcagg 2701 gccatgacaa tcgccaagct gaaaacaatg cggcaggaag attacatgaa gacgatagcc 2761 gaccttgagt tacattacca agagttcatc agaaatagcc aaggctcaga gatgtttgga 2821 gatgatgaca agcggaaaat acagtctcag ttcaccgatg cccagaagca ttaccagacc 2881 ctggtcattc agctccctgg ctatccccag caccagacag tgaccacaac tgaaatcact 2941 catcatggaa cctgccaaga tgtcaaccat aataaagtaa ttgaaaccaa cagagaaaat 3001 gacaagcaag aaacatggat gctgatggag ctgcagaaga ttcgcaggca gatagagcac 3061 tgcgagggca ggatgactct caaaaacctc cctctagcag accaggggtc ttctcaccac 3121 atcacagtga aaattaacga gcttaagagt gtgcagaatg attcacaagc aattgctgag 3181 gttctcaacc agcttaaaga tatgcttgcc aacttcagag gttctgaaaa gtactgctat 3241 ttacagaatg aagtatttgg actatttcag aaactggaaa atatcaatgg tgttacagat 3301 ggctacttaa atagcttatg cacagtaagg gcactgctcc aggctattct ccaaacagaa 3361 gacatgttaa aggtttatga agccaggctc actgaggagg aaactgtctg cctggacctg 3421 gataaagtgg aagcttaccg ctgtggactg aagaaaataa aaaatgactt gaacttgaag 3481 aagtcgttgt tggccactat gaagacagaa ctacagaaag cccagcagat ccactctcag 3541 acttcacagc agtatccact ttatgatctg gacttgggca agttcggtga aaaagtcaca 3601 cagctgacag accgctggca aaggatagat aaacagatcg actttagatt atgggacctg 3661 gagaaacaaa tcaagcaatt gaggaattat cgtgataact atcaggcttt ctgcaagtgg 3721 ctctatgatg ctaaacgccg ccaggattcc ttagaatcca tgaaatttgg agattccaac 3781 acagtcatgc ggtttttgaa tgagcagaag aacttgcaca gtgaaatatc tggcaaacga 3841 gacaaatcag aggaagtaca aaaaattgct gaactttgcg ccaattcaat taaggattat 3901 gagctccagc tggcctcata cacctcagga ctggaaactc tgctgaacat acctatcaag 3961 aggaccatga ttcagtcccc ttctggggtg attctgcaag aggctgcaga tgttcatgct 4021 cggtacattg aactacttac aagatctgga gactattaca ggttcttaag tgagatgctg 4081 aagagtttgg aagatctgaa gctgaaaaat accaagatcg aagttttgga agaggagctc 4141 agactggccc gagatgccaa ctcggaaaac tgtaataaga acaaattcct ggatcagaac 4201 ctgcagaaat accaggcaga gtgttcccag ttcaaagcga agcttgcgag cctggaggag 4261 ctgaagagac aggctgagct ggatgggaag tcggctaagc aaaatctaga caagtgctac 4321 ggccaaataa aagaactcaa tgagaagatc acccgactga cttatgagat tgaagatgaa 4381 aagagaagaa gaaaatctgt ggaagacaga tttgaccaac agaagaatga ctatgaccaa 4441 ctgcagaaag caaggcaatg tgaaaaggag aaccttggtt ggcagaaatt agagtctgag 4501 aaagccatca aggagaagga gtacgagatt gaaaggttga gggttctact gcaggaagaa 4561 ggcacccgga agagagaata tgaaaatgag ctggcaaagg taagaaacca ctataatgag 4621 gagatgagta atttaaggaa caagtatgaa acagagatta acattacgaa gaccaccatc 4681 aaggagatat ccatgcaaaa agaggatgat tccaaaaatc ttagaaacca gcttgataga 4741 ctttcaaggg aaaatcgaga tctgaaggat gaaattgtca ggctcaatga cagcatcttg 4801 caggccactg agcagcgaag gcgagctgaa gaaaacgccc ttcagcaaaa ggcctgtggc 4861 tctgagataa tgcagaagaa gcagcatctg gagatagaac tgaagcaggt catgcagcag 4921 cgctctgagg acaatgcccg gcacaagcag tccctggagg aggctgccaa gaccattcag 4981 gacaaaaata aggagatcga gagactcaaa gctgagtttc aggaggaggc caagcgccgc 5041 tgggaatatg aaaatgaact gagtaaggta agaaacaatt atgatgagga gatcattagc 5101 ttaaaaaatc agtttgagac cgagatcaac atcaccaaga ccaccatcca ccagctcacc 5161 atgcagaagg aagaggatac cagtggctac cgggctcaga tagacaatct cacccgagaa 5221 aacaggagct tatctgaaga aataaagagg ctgaagaaca ctctaaccca gaccacagag 5281 aatctcagga gggtggaaga agacatccaa cagcaaaagg ccactggctc tgaggtgtct 5341 cagaggaaac agcagctgga ggttgagctg agacaagtca ctcagatgcg aacagaggag 5401 agcgtaagat ataagcaatc tcttgatgat gctgccaaaa ccatccagga taaaaacaag 5461 gagatagaaa ggttaaaaca actgatcgac aaagaaacaa atgaccggaa atgcctggaa 5521 gatgaaaacg cgagattaca aagggtccag tatgacctgc agaaagcaaa cagtagtgcg 5581 acggagacaa taaacaaact gaaggttcag gagcaagaac tgacacgcct gaggatcgac 5641 tatgaaaggg tttcccagga gaggactgtg aaggaccagg atatcacgcg gttccagaac 5701 tctctgaaag agctgcagct gcagaagcag aaggtggaag aggagctgaa tcggctgaag 5761 aggaccgcgt cagaagactc ctgcaagagg aagaagctgg aggaagagct ggaaggcatg 5821 aggaggtcgc tgaaggagca agccatcaaa atcaccaacc tgacccagca gctggagcag 5881 gcatccattg ttaagaagag gagtgaggat gacctccggc agcagaggga cgtgctggat 5941 ggccacctga gggaaaagca gaggacccag gaagagctga ggaggctctc ttctgaggtc 6001 gaggccctga ggcggcagtt actccaggaa caggaaagtg tcaaacaagc tcacttgagg 6061 aatgagcatt tccagaaggc gatagaagat aaaagcagaa gcttaaatga aagcaaaata 6121 gaaattgaga ggctgcagtc tctcacagag aacctgacca aggagcactt gatgttagaa 6181 gaagaactgc ggaacctgag gctggagtac gatgacctga ggagaggacg aagcgaagcg 6241 gacagtgata aaaatgcaac catcttggaa ctaaggagcc agctgcagat cagcaacaac 6301 cggaccctgg aactgcaggg gctgattaat gatttacaga gagagaggga aaatttgaga 6361 caggaaattg agaaattcca aaagcaggct ttagaggcat ctaataggat tcaggaatca 6421 aagaatcagt gtactcaggt ggtacaggaa agagagagcc ttctggtgaa aatcaaagtc 6481 ctggagcaag acaaggcaag gctgcagagg ctggaggatg agctgaatcg tgcaaaatca 6541 actctagagg cagaaaccag ggtgaaacag cgcctggagt gtgagaaaca gcaaattcag 6601 aatgacctga atcagtggaa gactcaatat tcccgcaagg aggaggctat taggaagata 6661 gaatcggaaa gagaaaagag tgagagagag aagaacagtc ttaggagtga gatcgaaaga 6721 ctccaagcag agatcaagag aattgaagag aggtgcaggc gtaagctgga ggattctacc 6781 agggagacac agtcacagtt agaaacagaa cgctcccgat atcagaggga gattgataaa 6841 ctcagacagc gcttaattaa gggaagcggc ggaaccggat cgactagcgg gggtagcggg 6901 ggaagcacgg ggggaggaac tggcgccgtg agcaagggcg aggagaccac aatgggcgta 6961 atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc 7021 gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag 7081 gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac 7141 ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc 7201 cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg 7261 aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag 7321 aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc 7381 gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg 7441 gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa gaaggcggtg 7501 aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca cgacaaggac 7561 tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga cggcatggac 7621 gagctgtaca aggggccagg tggtgcaggg ccaggtggtg cagggccagg tggtgcaggg 7681 ccaggtggtg cagggcccgg tggtgcaggt ccaggtggtg caggtccagg tggtgcaggt 7741 ccaggtggtg ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 7801 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 7861 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 7921 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc 7981 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 8041 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 8101 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 8161 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 8221 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 8281 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 8341 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc 8401 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 8461 ctgtacaagg gcaccggagg tggcactagc ggaggctcag gcggatcgac ctccggaact 8521 ggaggttccg gtagcggccg cccatatggg tcccatcgag agacccagac tgagtgtgag 8581 tggaccgttg acacctccaa gctggtgttt gatgggctga ggaagaaggt gacagcaatg 8641 cagctctatg agtgtcagct gatcgacaaa acaaccttgg acaaactatt gaaggggaag 8701 aagtcagtgg aagaagttgc ttctgaaatc cagccattcc ttcggggtgc aggatctatc 8761 gctggagcat ctgcttctcc taaggaaaaa tactctttgg tagaggccaa gagaaagaaa 8821 ttaatcagcc cagaatccac agtcatgctt ctggaggccc aggcagctac aggtggtata 8881 attgatcccc atcggaatga gaagctgact gtcgacagtg ccatagctcg ggacctcatt 8941 gacttcgatg accgtcagca gatatatgca gcagaaaaag ctatcactgg ttttgatgat 9001 ccattttcag gcaagacagt atctgtttca gaagccatca agaaaaattt gattgataga 9061 gaaaccggaa tgcgcctgct ggaagcccag attgcttcag ggggtgtagt agaccctgtg 9121 aacagtgtct ttttgccaaa agatgtcgcc ttggcccggg ggctgattga tagagatttg 9181 tatcgatccc tgaatgatcc ccgagatagt cagaaaaact ttgtggatcc agtcaccaaa 9241 aagaaggtca gttacgtgca gctgaaggaa cggtgcagaa tcgaaccaca tactggtctg 9301 ctcttgcttt cagtacagaa gagaagcatg tccttccaag gaatcagaca acctgtgacc 9361 gtcactgagc tagtagattc tggtatattg agaccgtcca ctgtcaatga actggaatct 9421 ggtcagattt cttatgacga ggttggtgag agaattaagg acttcctcca gggttcaagc 9481 tgcatagcag gcatatacaa tgagaccaca aaacagaagc ttggcattta tgaggccatg 9541 aaaattggct tagtccgacc tggtactgct ctggagttgc tggaagccca agcagctact 9601 ggctttatag tggatcctgt tagcaacttg aggttaccag tggaggaagc ctacaagaga 9661 ggtctggtgg gcattgagtt caaagagaag ctcctgtctg cagaacgagc tgtcactggg 9721 tataatgatc ctgaaacagg aaacatcatc tctttgttcc aagccatgaa taaggaactc 9781 atcgaaaagg gccacggtat tcgcttatta gaagcacaga tcgcaaccgg ggggatcatt 9841 gacccaaagg agagccatcg tttaccagtt gacatagcat ataagagggg ctatttcaat 9901 gaggaactca gtgagattct ctcagatcca agtgatgata ccaaaggatt ttttgacccc 9961 aacactgaag aaaatcttac ctatctgcaa ctaaaagaaa gatgcattaa ggatgaggaa 10021 acagggctct gtcttctgcc tctgaaagaa aagaagaaac aggtgcagac atcacaaaag 10081 aataccctca ggaagcgtag agtggtcata gttgacccag aaaccaataa agaaatgtct 10141 gttcaggagg cctacaagaa gggcctaatt gattatgaaa ccttcaaaga actgtgtgag 10201 caggaatgtg aatgggaaga aataaccatc acgggatcag atggctccac cagggtggtc 10261 ctggtagata gaaagacagg cagtcagtat gatattcaag atgctattga caagggcctt 10321 gttgacagga agttctttga tcagtaccga tccggcagcc tcagcctcac tcaatttgct 10381 gacatgatct ccttgaaaaa tggtgtcggc accagcagca gcatgggcag tggtgtcagc 10441 gatgatgttt ttagcagctc ccgacatgaa tcagtaagta agatttccac catatccagc 10501 gtcaggaatt taaccataag gagcagctct ttttcagaca ccctggaaga atcgagcccc 10561 attgcagcca tctttgacac agaaaacctg gagaaaatct ccattacaga aggtatagag 10621 cggggcatcg ttgacagcat cacgggtcag aggcttctgg aggctcaggc ctgcacaggt 10681 ggcatcatcc acccaaccac gggccagaag ctgtcacttc aggacgcagt ctcccagggt 10741 gtgattgacc aagacatggc caccaggctg aagcctgctc agaaagcctt cataggcttc 10801 gagggtgtga agggaaagaa gaagatgtca gcagcagagg cagtgaaaga aaaatggctc 10861 ccgtatgagg ctggccagcg cttcctggag ttccagtacc tcacgggagg tcttgttgac 10921 ccggaagtgc atgggaggat aagcaccgaa gaagccatcc ggaaggggtt catagatggc 10981 cgcgccgcac agaggctgca agacaccagc agctatgcca aaatcctgac ctgccccaaa 11041 accaaattaa aaatatccta taaggatgcc ataaatcgct ccatggtaga agatatcact 11101 gggctgcgcc ttctggaagc cgcctccgtg tcgtccaagg gcttacccag cccttacaac 11161 atgtcttcgg ctccggggtc ccgctccggc tcccgctcgg gatctcgctc cggatctcgc 11221 tccgggtccc gcagtgggtc ccggagagga ggctttgacg ccacagggaa ttcttcctac 11281 tcttattcct actcatttag cagtagttct attgggcact aggctagcgg cctgtcaggc 11341 caagcttcca tcgatagaca tgataagata cattgatgag tttggacaaa ccacaacaag 11401 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac 11461 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt 11521 tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaccta 11581 ggcgagaccc tgtctcacaa aataaagtaa gcccggactg agtgcggaaa ggcgggcctg 11641 gcgggtctgg tctccccatg cgggccacca gaggccctgc agccttcagt cgcttgaagg 11701 ggtaatggcg cttccactca caaacatggc ggacagagcg tgtgaacgag atgaacagcc 11761 cctcaaaaat atggccgccg aggctggacg gccgtgcccc agcagcaccg cctccgcgcc 11821 ccacgtgatc tctcgccggg cacagcgctg accgcggagg tccaaccgga agaatgtccg 11881 gattggacat tcggaagagg gcccgccttc cctggggaat ctctgcgcac gcgcagaacg 11941 cttcgaccaa tgaaaacaca ggaagccgtc cgcgcaaccg cgttgcgtca cttctgccgc 12001 ccctgtttca aggtatatag ccgtagacgg aacttcgcct ttctctcggc cttagcgcca 12061 tttttttggg tgagtgtttt ttggttcctg cgttgggatt ccgtgtacaa tccatagaca 12121 tctgacctcg gcacttagca tcatcacagc aaactaactg tagcctttct ctctttccct 12181 gtagaaacct ctgcacctga ggccaccatg tctagactgg acaagagcaa agtcataaac 12241 ggcgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct 12301 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg 12361 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa 12421 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cattccgctg tgctctcctc 12481 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa 12541 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg 12601 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat 12661 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga 12721 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa 12781 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc 12841 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat 12901 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtccggcgct 12961 actaacttca gcctgctgaa gcaggctggt gacgtcgagg agaatcctgg tcccatgacc 13021 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 13081 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 13141 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 13201 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 13261 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 13321 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 13381 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 13441 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 13501 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 13561 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgattcgaa 13621 ggcctgtcgt gaagcttggg gatcaattct ctagagctcg ctgatcagcc tcgactgtgc 13681 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 13741 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 13801 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 13861 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 13921 gctgggccta ctagctactc gggacccctt accgaaacat cgccgcattc tgcagaggag 13981 tcgagtgtat gtaaacttct gacccactgg gaatgtgatg aaagaaataa aagctgaaat 14041 gaatcattct ctctactatt attctgatat ttcacattct taaaataaag tggtgatcct 14101 aactgaccta agacagggaa tttttactag gattaaatgt caggaattgt gaaaaagtga 14161 gtttaaatgt atttggctaa ggtgtatgta aacttccgac ttcaactgta tagggatccg 14221 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 14281 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 14341 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 14401 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 14461 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 14521 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 14581 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 14641 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 14701 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 14761 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 14821 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 14881 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 14941 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 15001 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 15061 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 15121 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 15181 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 15241 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 15301 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 15361 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 15421 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 15481 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 15541 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 15601 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 15661 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 15721 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 15781 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 15841 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 15901 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 15961 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 16021 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 16081 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 16141 cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa 16201 ttgtaagcgt taatattttg ttaaaatt //