LOCUS       Au1-PYL_nPPVp           6381 bp    DNA     circular SYN 11-OCT-2018
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12504572|
COMMENT     VNTDBDATE|-12504575|
COMMENT     LSOWNER|
COMMENT     VNTNAME|Au1-PYL_nPPVp|
COMMENT     VNTAUTHORNAME|Estera Merljak|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 908 0 1518 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 939 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 890 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 611 0 949 0 2912 0 3647 0 
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COMMENT                          "Foreign object. Author: Estera Merljak. Original author: UNKNOWN" 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(1934..1951)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(1935..1952)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(1971..1988)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      1974..2201
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      2264..2570
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(2684..2704)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2690..2906)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2702..2971
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      2870..2947
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    2932..2951
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3086..3880
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            3089..3877
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      4057..4176
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    4145..4164
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(4220..4238)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(4237..4259)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(4273..4302)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(4611..5230)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(5385..6245)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(5385..6245)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(6287..6315)
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    971..1516
                     /vntifkey="21"
                     /label=PYL1
     misc_feature    997..997
                     /vntifkey="21"
                     /label=mutacija\EcoRI\mesta
     misc_feature    953..970
                     /vntifkey="21"
                     /label=Au1-tag
     misc_feature    1529..1882
                     /vntifkey="21"
                     /label=N-PPV
BASE COUNT     1493 a      1672 c      1645 g      1571 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tggacaccta 
      961 caggtacatc ggtgggggcg cgccaactca agacgagttc acccaactct cccaatcaat 
     1021 cgccgagttc cacacgtacc aactcggtaa cggccgttgc tcatctctcc tagctcagcg 
     1081 aatccacgcg ccgccggaaa cagtatggtc cgtggtgaga cgtttcgata ggccacagat 
     1141 ttacaaacac ttcatcaaaa gctgtaacgt gagtgaagat ttcgagatgc gagtgggatg 
     1201 cacgcgcgac gtgaacgtga taagtggatt accggcgaat acgtctcgag agagattaga 
     1261 tctgttggac gatgatcgga gagtgactgg gtttagtata accggtggtg aacataggct 
     1321 gaggaattat aaatcggtta cgacggttca tagatttgag aaagaagaag aagaagaaag 
     1381 gatctggacc gttgttttgg aatcttatgt tgttgatgta ccggaaggta attcggagga 
     1441 agatacgaga ttgtttgctg atacggttat tagattgaat cttcagaaac ttgcttcgat 
     1501 cactgaagct atgaacggat ccggaagtag caagagcctg ttccgcggcc tgcgcgacta 
     1561 caaccccatc gccagcagca tctgccagct gaacaacagc agcggcgccc gccagagcga 
     1621 gatgttcggc ctgggcttcg gcggcctgat cgtgaccaac cagcacctgt tcaagcgcaa 
     1681 cgacggcgag ctgaccatcc gcagccacca cggcgagttc gtggtgaagg acaccaagac 
     1741 cctgaagctg ctgccctgca agggccgcga catcgtgatc atccgcctgc ccaaggactt 
     1801 cccccccttc cccaagcgcc tgcaattccg cacccccacc accgaggacc gcgtgtgcct 
     1861 gatcggcagc aacttccaga cctaaactag tgcggccgcc tgcagctcga gcatgcatct 
     1921 agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt 
     1981 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 
     2041 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 
     2101 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 
     2161 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac 
     2221 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg 
     2281 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 
     2341 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 
     2401 gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 
     2461 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 
     2521 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 
     2581 tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct attggttaaa 
     2641 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta 
     2701 gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa 
     2761 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     2821 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 
     2881 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     2941 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg 
     3001 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca 
     3061 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 
     3121 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 
     3181 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 
     3241 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac 
     3301 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 
     3361 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 
     3421 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 
     3481 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 
     3541 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 
     3601 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 
     3661 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 
     3721 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 
     3781 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 
     3841 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 
     3901 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 
     3961 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 
     4021 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 
     4081 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     4141 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 
     4201 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     4261 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     4321 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     4381 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     4441 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     4501 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     4561 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     4621 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     4681 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     4741 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     4801 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     4861 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     4921 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     4981 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     5041 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     5101 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     5161 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     5221 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     5281 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     5341 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     5401 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     5461 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     5521 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     5581 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     5641 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     5701 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     5761 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     5821 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     5881 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     5941 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     6001 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     6061 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     6121 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     6181 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     6241 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     6301 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     6361 cgaaaagtgc cacctgacgt c 
//
