LOCUS       pCMV_ABI_cTEVp          6739 bp    DNA     circular SYN 21-OCT-2018
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12518704|
COMMENT     VNTDBDATE|-12518704|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCMV_ABI_cTEVp|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(2292..2309)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(2293..2310)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(2329..2346)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      2332..2559
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      2622..2928
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(3042..3062)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3048..3264)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3060..3329
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      3228..3305
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    3290..3309
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3444..4238
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            3447..4235
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      4415..4534
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    4503..4522
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(4578..4596)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(4595..4617)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(4631..4660)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(4969..5588)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(5743..6603)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(5743..6603)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(6645..6673)
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    2273..2275
                     /vntifkey="21"
                     /label=STOP
     misc_feature    953..982
                     /vntifkey="21"
                     /label=Myc
     misc_feature    983..1882
                     /vntifkey="21"
                     /label=ABI
     misc_feature    1901..2272
                     /vntifkey="21"
                     /label=CTEV
BASE COUNT     1584 a      1662 c      1776 g      1717 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tggagcagaa 
      961 gctgatcagc gaggaggatc tgacgcgtgt gcctttgtat ggttttactt cgatttgtgg 
     1021 aagaagacct gagatggaag ctgctgtttc gactatacca agattccttc aatcttcctc 
     1081 tggttcgatg ttagatggtc ggtttgatcc tcaatccgcc gctcatttct tcggtgttta 
     1141 cgacggccat ggcggttctc aggtagcgaa ctattgtaga gagaggatgc atttggcttt 
     1201 ggcggaggag atagctaagg agaaaccgat gctctgcgat ggtgatacgt ggctggagaa 
     1261 gtggaagaaa gctcttttca actcgttcct gagagttgac tcggagattg agtcagttgc 
     1321 gccggagacg gttgggtcaa cgtcggtggt tgccgttgtt ttcccgtctc acatcttcgt 
     1381 cgctaactgc ggtgactcta gagccgttct ttgccgcggc aaaactgcac ttccattatc 
     1441 cgttgaccat aaaccggata gagaagatga agctgcgagg attgaagccg caggagggaa 
     1501 agtgattcag tggaatggag ctcgtgtttt cggtgttctc gccatgtcga gatccattgg 
     1561 cgatagatac ttgaaaccat ccatcattcc tgatccggaa gtgacggctg tgaagagagt 
     1621 aaaagaagat gattgtctga ttttggcgag tgacggggtt tgggatgtaa tgacggatga 
     1681 agaagcgtgt gagatggcaa ggaagcggat tctcttgtgg cacaagaaaa acgcggtggc 
     1741 tggggatgca tcgttgctcg cggatgagcg gagaaaggaa gggaaagatc ctgcggcgat 
     1801 gtccgcggct gagtatttgt caaagctggc gatacagaga ggaagcaaag acaacataag 
     1861 tgtggtggtg gttgatttga agggatccgg ctccggcagc aagagcatgt ctagcatggt 
     1921 gtcagacacc agttgcacat tcccttcatc tgatggcata ttctggaagc attggattca 
     1981 aaccaaggat gggcagtgtg gcagtccatt agtatcaact agagatgggt tcattgttgg 
     2041 tatacactca gcatcgaatt tcaccaacac aaacaattat ttcacaagcg tgccgaaaaa 
     2101 cttcatggaa ttgttgacaa atcaggaggc gcagcagtgg gttagtggtt ggcgattaaa 
     2161 tgctgactca gtattgtggg ggggccataa agttttcatg agcaaacctg aagagccttt 
     2221 tcagccagtt aaggaagcga ctcaactcat gagtgaattg gtgtactcgc aataatctag 
     2281 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc 
     2341 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 
     2401 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 
     2461 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 
     2521 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 
     2581 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     2641 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     2701 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     2761 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     2821 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     2881 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
     2941 tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa 
     3001 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 
     3061 gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt 
     3121 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 
     3181 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 
     3241 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     3301 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     3361 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 
     3421 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 
     3481 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 
     3541 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 
     3601 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 
     3661 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 
     3721 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 
     3781 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 
     3841 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 
     3901 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 
     3961 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 
     4021 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 
     4081 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 
     4141 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 
     4201 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 
     4261 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 
     4321 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 
     4381 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 
     4441 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     4501 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 
     4561 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     4621 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     4681 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     4741 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     4801 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     4861 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     4921 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     4981 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     5041 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     5101 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     5161 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     5221 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     5281 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     5341 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     5401 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
     5461 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     5521 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     5581 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     5641 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     5701 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     5761 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     5821 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     5881 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     5941 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     6001 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     6061 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     6121 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     6181 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     6241 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     6301 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     6361 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     6421 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     6481 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     6541 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     6601 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     6661 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     6721 aaaagtgcca cctgacgtc 
//
