LOCUS       Exported               11477 bp ds-DNA     circular SYN 17-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pTn7C19_20151206
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Jason X. M. Peters
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 17, 2018 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11477
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(58..918)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="Bla"
                     /note="bla"
                     /db_xref="GI:403391486"
                     /protein_id="AFR43358.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(919..1023)
                     /gene="bla"
                     /note="AmpR promoter"
     mobile_element  1501..1698
                     /mobile_element_type="transposon:Tn7R"
                     /note="Tn7R"
     primer_bind     1703..1719
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1747..1841
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      1944..2030
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    2081..2128
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(2196..2432)
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /note="TpR"
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(2757..2785)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /note="Pc promoter"
                     /note="class 1 integron promoter"
     protein_bind    2876..2923
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(2924..3013)
                     /note="spy terminator"
     CDS             complement(3061..3738)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /note="mRFP1"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    3595..3614
                     /note="RR1"
     RBS             3745..3756
                     /note="RBS"
     RBS             3745..3756
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(3766..3800)
                     /note="J23119 (BglII) promoter"
     terminator      complement(3869..3919)
                     /note="L3S1P13 terminator"
     ncRNA           complement(3968..4060)
                     /note="optimized sgRNA"
                     /ncRNA_class="other"
     misc_feature    4061..4080
                     /note="RR1"
     misc_feature    complement(4086..4115)
                     /note="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      complement(4192..4248)
                     /note="thrLABC terminator"
     terminator      complement(4249..4302)
                     /note="garPLRK-rnpB terminator"
     terminator      4389..4441
                     /note="hisLGDCBHAFI terminator"
     terminator      4442..4490
                     /note="pdhR-aceEF-lpd terminator"
     CDS             complement(4537..5415)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /note="araC"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5442..5726
                     /gene="araBAD"
                     /note="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             5753..5775
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5787..9890
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /note="dCas9"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9893..9992
                     /codon_start=1
                     /note="3xMyc"
                     /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL"
     terminator      10024..10078
                     /note="L3S2P24 terminator"
     mobile_element  10145..10310
                     /mobile_element_type="transposon:Tn7"
                     /note="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            10469..10577
                     /note="incP origin of transfer"
     rep_origin      10846..11234
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
       61 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      181 tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag caataaacca
      241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
      541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1021 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     1081 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     1141 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     1201 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     1261 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     1321 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     1381 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctag aggaccagcc
     1441 gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt
     1501 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc
     1561 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata
     1621 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg
     1681 tattaaagag gggcgtgggg ttcgaggtcg acggtatcga taagctagct taattagctg
     1741 agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     1801 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagctag actgcgatga
     1861 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggggt
     1921 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1981 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgctag
     2041 gagcttgcgg cccggacgat gagctcgaat tggggatctt gaagtaccta ttccgaagtt
     2101 cctattctct agaaagtata ggaacttcag agcgcttttg aagctgatgc tcgagtgctt
     2161 aaaaacttac tcaatggaat aattctagat aattcttagg ccacacgttc aagtgcagcc
     2221 acaggataaa tttgcactga gcctgggtgg gattcggact cgaccgcata gccttcagga
     2281 gtgagttttg tgcaatacca accgacgact tgaccctgcc aagcggcacc agatttcttg
     2341 cgtacgcgat cccctaagcc aaaggtggca ctcaggggaa gcgcaaactg ccctgcaacg
     2401 ggagcgttgg cttcatcgct actttgaccc atgtcgaatc cttcttgtga atctattatg
     2461 gcgacaagca aatcgagctc tgactgccta ccccacaaca actatcagaa agcaccagca
     2521 caacggctgc ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
     2581 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggctag
     2641 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     2701 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     2761 tacgaaccga acaggcttat gtcaactggg ttcgtgaatt atccattgct gttgacaaag
     2821 ggaatcaggc tcgagagttc aacagacagc cttcgttctt atggccgttc aggaggaagt
     2881 tcctattccg aagttcctat tctctagaaa gtataggaac ttcttgagaa gagaaaagaa
     2941 aaccgccgat cctgtccacc gcattactgc aaggtagtgg acaagaccgg cggtcttaag
     3001 ttttttggct gaaacgaatg acgaaaaggc tgtctgagca aatccaggag gtcgttttta
     3061 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
     3121 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
     3181 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
     3241 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
     3301 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
     3361 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
     3421 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
     3481 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
     3541 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
     3601 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
     3661 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
     3721 gtcttcgcta ctcgccatgg tacctttctc ctctttaatt aattcagatc tattatacct
     3781 aggactgagc tagctgtcaa attcaccacc ctgaattgac tctcaaaccg tattagcccg
     3841 gtattttgga aatagcggaa gcactgactt ttgttatcaa taaaaaaggc cccccgttag
     3901 ggaggcctta ttgttcgtct actcggaaga gcgagagaca acagaacggt cagccacatg
     3961 aattcaaaaa aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt
     4021 atttaaactt gctatgctgt ttccagcata gctcttaaac agaccgctaa actgaaagtt
     4081 ccacacatta tacgagccgg atgattaatt gtcaacagct catttcagaa tatttgccag
     4141 aaccggaatt ctttcagctc agtcgatagg tagtaggcaa gagtagtcgc acctttggtc
     4201 gaaaaaaaaa gcccgcactg tcaggtgcgg gcttttttct gtgtttcccc aaaagtaaaa
     4261 acccgccgaa gcgggttttt acgtaaaaca ggtgaaactg accagacgag aaggctttgg
     4321 aggtgataat ggggctcaag gacccgggag gcagataggt aggcatggcc cccattttca
     4381 atacaagcaa cgcatgagaa agcccccgga agatcacctt ccgggggctt ttttattgcg
     4441 ctccggcaat taaaaaagcg gctaaccacg ccgctttttt tacgtctgca gagctcatag
     4501 gcaagcgaat cgtgatgcct cttagccagt actagtttat gacaacttga cggctacatc
     4561 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
     4621 aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
     4681 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
     4741 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
     4801 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
     4861 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
     4921 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
     4981 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
     5041 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc
     5101 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
     5161 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
     5221 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
     5281 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
     5341 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
     5401 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
     5461 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt
     5521 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     5581 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     5641 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     5701 atcgcaactc tctactgttt ctccataccc gtttttttgg gctagaaata attttgttta
     5761 actttaagaa ggagatatac atacccatgg ataagaaata ctcaataggc ttagctatcg
     5821 gcacaaatag cgtcggatgg gcggtgatca ctgatgaata taaggttccg tctaaaaagt
     5881 tcaaggttct gggaaataca gaccgccaca gtatcaaaaa aaatcttata ggggctcttt
     5941 tatttgacag tggagagaca gcggaagcga ctcgtctcaa acggacagct cgtagaaggt
     6001 atacacgtcg gaagaatcgt atttgttatc tacaggagat tttttcaaat gagatggcga
     6061 aagtagatga tagtttcttt catcgacttg aagagtcttt tttggtggaa gaagacaaga
     6121 agcatgaacg tcatcctatt tttggaaata tagtagatga agttgcttat catgagaaat
     6181 atccaactat ctatcatctg cgaaaaaaat tggtagattc tactgataaa gcggatttgc
     6241 gcttaatcta tttggcctta gcgcatatga ttaagtttcg tggtcatttt ttgattgagg
     6301 gagatttaaa tcctgataat agtgatgtgg acaaactatt tatccagttg gtacaaacct
     6361 acaatcaatt atttgaagaa aaccctatta acgcaagtgg agtagatgct aaagcgattc
     6421 tttctgcacg attgagtaaa tcaagacgat tagaaaatct cattgctcag ctccccggtg
     6481 agaagaaaaa tggcttattt gggaatctca ttgctttgtc attgggtttg acccctaatt
     6541 ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca gctttcaaaa gatacttacg
     6601 atgatgattt agataattta ttggcgcaaa ttggagatca atatgctgat ttgtttttgg
     6661 cagctaagaa tttatcagat gctattttac tttcagatat cctaagagta aatactgaaa
     6721 taactaaggc tcccctatca gcttcaatga ttaaacgcta cgatgaacat catcaagact
     6781 tgactctttt aaaagcttta gttcgacaac aacttccaga aaagtataaa gaaatctttt
     6841 ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg gggagctagc caagaagaat
     6901 tttataaatt tatcaaacca attttagaaa aaatggatgg tactgaggaa ttattggtga
     6961 aactaaatcg tgaagatttg ctgcgcaagc aacggacctt tgacaacggc tctattcccc
     7021 atcaaattca cttgggtgag ctgcatgcta ttttgagaag acaagaagac ttttatccat
     7081 ttttaaaaga caatcgtgag aagattgaaa aaatcttgac ttttcgaatc ccttattatg
     7141 ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat gactcggaag tctgaagaaa
     7201 caattacccc atggaatttt gaagaagttg tcgataaagg tgcttcagct caatcattta
     7261 ttgaacgcat gacaaacttt gataaaaatc ttccaaatga aaaagtacta ccaaaacata
     7321 gtttgcttta tgagtatttt acggtttata acgaattgac aaaggtcaaa tatgttactg
     7381 aaggaatgcg aaaaccagca tttctttcag gtgaacagaa gaaagccatt gttgatttac
     7441 tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa agaagattat ttcaaaaaaa
     7501 tagaatgttt tgatagtgtt gaaatttcag gagttgaaga tagatttaat gcttcattag
     7561 gtacctacca tgatttgcta aaaattatta aagataaaga ttttttggat aatgaagaaa
     7621 atgaagatat cttagaggat attgttttaa cattgacctt atttgaagat agggagatga
     7681 ttgaggaaag acttaaaaca tatgctcacc tctttgatga taaggtgatg aaacagctta
     7741 aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa attgattaat ggtattaggg
     7801 ataagcaatc tggcaaaaca atattagatt ttttgaaatc agatggtttt gccaatcgca
     7861 attttatgca gctgatccat gatgatagtt tgacatttaa agaagacatt caaaaagcac
     7921 aagtgtctgg acaaggcgat agtttacatg aacatattgc aaatttagct ggtagccctg
     7981 ctattaaaaa aggtatttta cagactgtaa aagttgttga tgaattggtc aaagtaatgg
     8041 ggcggcataa gccagaaaat atcgttattg aaatggcacg tgaaaatcag acaactcaaa
     8101 agggccagaa aaattcgcga gagcgtatga aacgaatcga agaaggtatc aaagaattag
     8161 gaagtcagat tcttaaagag catcctgttg aaaatactca attgcaaaat gaaaagctct
     8221 atctctatta tctccaaaat ggaagagaca tgtatgtgga ccaagaatta gatattaatc
     8281 gtttaagtga ttatgatgtc gatgccattg ttccacaaag tttccttaaa gacgattcaa
     8341 tagacaataa ggtcttaacg cgttctgata aaaatcgtgg taaatcggat aacgttccaa
     8401 gtgaagaagt agtcaaaaag atgaaaaact attggagaca acttctaaac gccaagttaa
     8461 tcactcaacg taagtttgat aatttaacga aagctgaacg tggaggtttg agtgaacttg
     8521 ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg ccaaatcact aagcatgtgg
     8581 cacaaatttt ggatagtcgc atgaatacta aatacgatga aaatgataaa cttattcgag
     8641 aggttaaagt gattacctta aaatctaaat tagtttctga cttccgaaaa gatttccaat
     8701 tctataaagt acgtgagatt aacaattacc atcatgccca tgatgcgtat ctaaatgccg
     8761 tcgttggaac tgctttgatt aagaaatatc caaaacttga atcggagttt gtctatggtg
     8821 attataaagt ttatgatgtt cgtaaaatga ttgctaagtc tgagcaagaa ataggcaaag
     8881 caaccgcaaa atatttcttt tactctaata tcatgaactt cttcaaaaca gaaattacac
     8941 ttgcaaatgg agagattcgc aaacgccctc taatcgaaac taatggggaa actggagaaa
     9001 ttgtctggga taaagggcga gattttgcca cagtgcgcaa agtattgtcc atgccccaag
     9061 tcaatattgt caagaaaaca gaagtacaga caggcggatt ctccaaggag tcaattttac
     9121 caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga ctgggatcca aaaaaatatg
     9181 gtggttttga tagtccaacg gtagcttatt cagtcctagt ggttgctaag gtggaaaaag
     9241 ggaaatcgaa gaagttaaaa tccgttaaag agttactagg gatcacaatt atggaaagaa
     9301 gttcctttga aaaaaatccg attgactttt tagaagctaa aggatataag gaagttaaaa
     9361 aagacttaat cattaaacta cctaaatata gtctttttga gttagaaaac ggtcgtaaac
     9421 ggatgctggc tagtgccgga gaattacaaa aaggaaatga gctggctctg ccaagcaaat
     9481 atgtgaattt tttatattta gctagtcatt atgaaaagtt gaagggtagt ccagaagata
     9541 acgaacaaaa acaattgttt gtggagcagc ataagcatta tttagatgag attattgagc
     9601 aaatcagtga attttctaag cgtgttattt tagcagatgc caatttagat aaagttctta
     9661 gtgcatataa caaacataga gacaaaccaa tacgtgaaca agcagaaaat attattcatt
     9721 tatttacgtt gacgaatctt ggagctcccg ctgcttttaa atattttgat acaacaattg
     9781 atcgtaaacg atatacgtct acaaaagaag ttttagatgc cactcttatc catcaatcca
     9841 tcactggtct ttatgaaaca cgcattgatt tgagtcagct aggaggtgac gcggccgcgg
     9901 agcagaaact catctctgaa gaagatctgg aacaaaagtt gatttcagaa gaagatctgg
     9961 aacagaagct catctctgag gaagatctgt aataaggcgc gcctccttaa tgggacttgc
    10021 agcctcggta ccaaattcca gaaaagacac ccgaaagggt gttttttcgt tttggtccca
    10081 cagaatgagc atcatggcac tagtcgacat cggagaagag tacggctctt ttaaccgcct
    10141 caagaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt
    10201 agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta
    10261 aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt
    10321 tttcttcctg ttatgtttgg gcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10381 gtttgcgtat tgggcgctct tccgcttcct cgctcactga cccgctgcgc tcggtcgttc
    10441 ggctgcggcg agcggtatca gagcttatcg gccagcctcg cagagcagga ttcccgttga
    10501 gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta
    10561 cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc
    10621 tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa
    10681 tgaccccgaa gcagggttat gcagcggaaa gtatacctta aggaatcccc tgataacgca
    10741 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcactc
    10801 cctgcctctg tcatcacgat actgtgatgc catggctaat tcccatgtca gccgttaagt
    10861 gttcctgtgt cactcaaaat tgctttgaga ggctctaagg gcttctcagt gcgttacatc
    10921 cctggcttgt tgtccacaac cgttaaacct taaaagcttt aaaagcctta tatattcttt
    10981 tttttcttat aaaacttaaa accttagagg ctatttaagt tgctgattta tattaatttt
    11041 attgttcaaa catgagagct tagtacgtga aacatgagag cttagtacgt tagccatgag
    11101 agcttagtac gttagccatg agggtttagt tcgttaaaca tgagagctta gtacgttaaa
    11161 catgagagct tagtacgtga aacatgagag cttagtacgt actatcaaca ggttgaactg
    11221 ctgatcttca gatcctctac gccggacgca tcgtggccgg atcttgcggc cgcaaaaatt
    11281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11341 aatgcttaat cagtgaggca ccaataactg cctttgatct tttctacggg gtctgacgct
    11401 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    11461 acctagatcc ttttaaa
//
