LOCUS Exported 11839 bp ds-DNA circular SYN 18-OCT-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTn7C74_20160103 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11839) AUTHORS John Hawkins TITLE Direct Submission JOURNAL Exported Thursday, Oct 18, 2018 from SnapGene 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11839 /organism="synthetic DNA construct" /mol_type="other DNA" oriT 8..116 /note="incP origin of transfer" rep_origin 385..773 /note="R6K gamma ori" /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS complement(1074..1934) /codon_start=1 /transl_table=11 /gene="bla" /product="Bla" /note="bla" /db_xref="GI:403391486" /protein_id="AFR43358.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1935..2039) /gene="bla" /note="AmpR promoter" mobile_element 2517..2714 /mobile_element_type="transposon:Tn7R" /note="Tn7R" primer_bind 2719..2735 /note="KS primer" /note="common sequencing primer, one of multiple similar variants" terminator 2763..2857 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" terminator 2960..3046 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 3097..3144 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(3192..3725) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /note="GmR" /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3914..3942) /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" protein_bind 4059..4106 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." terminator complement(4107..4196) /note="spy terminator" CDS complement(4244..4921) /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /note="mRFP1" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" misc_feature 4778..4797 /note="RR1" RBS 4928..4939 /note="RBS" RBS 4928..4939 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" promoter complement(4949..4983) /note="J23119 (BglII) promoter" terminator complement(5052..5102) /note="L3S1P13 terminator" ncRNA complement(5151..5243) /note="optimized sgRNA" /ncRNA_class="other" misc_feature 5244..5263 /note="RR1" promoter complement(5270..5318) /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 5276..5292 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 5299..5313 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." terminator complement(5375..5431) /note="thrLABC terminator" terminator complement(5432..5485) /note="garPLRK-rnpB terminator" protein_bind 5540..5561 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." CDS complement(5574..6656) /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(6657..6734) /gene="lacI (mutant)" /note="lacIq promoter" /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." terminator 6798..6850 /note="hisLGDCBHAFI terminator" terminator 6851..6899 /note="pdhR-aceEF-lpd terminator" promoter 7081..7129 /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 7086..7100 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 7107..7123 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 7165..11268 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="dCas9" /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11271..11370 /codon_start=1 /note="3xMyc" /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL" terminator 11402..11456 /note="L3S2P24 terminator" mobile_element 11523..11688 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag 61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct 121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc 181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg 241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa 301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata 361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt 421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc 481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa 541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt 601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga 661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa 721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg 781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa 841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt 2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg 2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct 2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac 2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg 2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg 2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc 3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta 3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gatctcggct 3181 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt 3241 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag 3301 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca 3361 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc 3421 ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg 3481 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc 3541 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca 3601 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt 3661 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt 3721 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt 3781 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac 3841 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg 3901 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat 3961 ccgtttccac ggtgtgcgtc catgggcaaa tattatacgc aaggcgacaa ggctcgagag 4021 ttcaacagac agccttcgtt cttatggccg ttcaggagga agttcctatt ccgaagttcc 4081 tattctctag aaagtatagg aacttcttga gaagagaaaa gaaaaccgcc gatcctgtcc 4141 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaacga 4201 atgacgaaaa ggctgtctga gcaaatccag gaggtcgttt ttattaagca ccggtggagt 4261 gacgaccttc agcacgttcg tactgttcaa cgatggtgta gtcttcgttg tgggaggtga 4321 tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg aaccggtttt ttagccatgt 4381 aggtggtttt aacttcagcg tcgtagtgac caccgtcttt cagtttcaga cgcattttga 4441 tttcaccttt cagagcaccg tcttccgggt acatacgttc ggtggaagct tcccaaccca 4501 tggttttttt ctgcataacc ggaccgtcgg acgggaagtt ggtaccacgc agtttaactt 4561 tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt aacggtaaca acaccaccgt 4621 cttcgaagtt cataacacgt tcccatttga aaccttccgg gaaggacagt ttcaggtagt 4681 ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc gtactggaac tgcggggaca 4741 ggatgtccca agcgaacggc agcggaccac ctttggtaac tttcagttta gcggtctggg 4801 taccttcgta cggacgacct tcaccttcac cttcgatttc gaactcgtga ccgttaacgg 4861 aaccttccat acgaactttg aaacgcatga actctttgat aacgtcttcg ctactcgcca 4921 tggtaccttt ctcctcttta attaattcag atctattata cctaggactg agctagctgt 4981 caaattcacc accctgaatt gactctcaaa ccgtattagc ccggtatttt ggaaatagcg 5041 gaagcactga cttttgttat caataaaaaa ggccccccgt tagggaggcc ttattgttcg 5101 tctactcgga agagcgagag acaacagaac ggtcagccac atgaattcaa aaaaaaagca 5161 ccgactcggt gccacttttt caagttgata acggactagc cttatttaaa cttgctatgc 5221 tgtttccagc atagctctta aacagaccgc taaactgaaa gttactagaa gtatcttgtt 5281 atccgctcac aatgtcaatg ttatccgctc acatttatag atctttagga attctttcag 5341 ctcagtcgat aggtagtagg caagagtagt cgcacctttg gtcgaaaaaa aaagcccgca 5401 ctgtcaggtg cgggcttttt tctgtgtttc cccaaaagta aaaacccgcc gaagcgggtt 5461 tttacgtaaa acaggtgaaa ctgaccagac gagaaggctt tggaggtgat aatggggctc 5521 aaggaccctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 5581 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 5641 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc 5701 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg 5761 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag 5821 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac 5881 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg 5941 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag 6001 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca 6061 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg 6121 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata 6181 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag 6241 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg 6301 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct 6361 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca 6421 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt 6481 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct 6541 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg 6601 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc 6661 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac 6721 cattcgatgg tgtcaacgta aatgcatgcc gcttgggagg cagataggta ggcatggccc 6781 ccattttcaa tacaagcaac gcatgagaaa gcccccggaa gatcaccttc cgggggcttt 6841 tttattgcgc tccggcaatt aaaaaagcgg ctaaccacgc cgcttttttt acgtctgcag 6901 gcgaattgat ctggtttgac agcttatcac caagccagtt acctcggttc aaagagttgg 6961 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag 7021 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt 7081 ataaatgtga gcggataaca ttgacattgt gagcggataa caagatactg agcacatcag 7141 caggtttcac acaggaaaac tagtatggat aagaaatact caataggctt agctatcggc 7201 acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc 7261 aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta 7321 tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat 7381 acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa 7441 gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag 7501 catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat 7561 ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc 7621 ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga 7681 gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac 7741 aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt 7801 tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag 7861 aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt 7921 aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat 7981 gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca 8041 gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata 8101 actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg 8161 actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt 8221 gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt 8281 tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa 8341 ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat 8401 caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt 8461 ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaatccc ttattatgtt 8521 ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca 8581 attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt 8641 gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt 8701 ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa 8761 ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc 8821 ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata 8881 gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt 8941 acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat 9001 gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt 9061 gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa 9121 cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat 9181 aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat 9241 tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa 9301 gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct 9361 attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg 9421 cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag 9481 ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga 9541 agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat 9601 ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt 9661 ttaagtgatt atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata 9721 gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt 9781 gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc 9841 actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat 9901 aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca 9961 caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag 10021 gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc 10081 tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc 10141 gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat 10201 tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca 10261 accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt 10321 gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt 10381 gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc 10441 aatattgtca agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca 10501 aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt 10561 ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg 10621 aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt 10681 tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa 10741 gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg 10801 atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat 10861 gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac 10921 gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa 10981 atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt 11041 gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta 11101 tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat 11161 cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc 11221 actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgacgc ggccgcggag 11281 cagaaactca tctctgaaga agatctggaa caaaagttga tttcagaaga agatctggaa 11341 cagaagctca tctctgagga agatctgtaa taaggcgcgc ctccttaatg ggacttgcag 11401 cctcggtacc aaattccaga aaagacaccc gaaagggtgt tttttcgttt tggtcccaca 11461 gaatgagcat catggctcta gtcgacatcg gagaagagta cggctctttt aaccgcctca 11521 agaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag 11581 cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa 11641 aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt 11701 tcttcctgtt atgtttgggc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 11761 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgacc cgctgcgctc ggtcgttcgg 11821 ctgcggcgag cggtatcag //