LOCUS       Exported               11839 bp ds-DNA     circular SYN 18-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pTn7C74_20160103
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   John Hawkins
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 18, 2018 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11839
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     oriT            8..116
                     /note="incP origin of transfer"
     rep_origin      385..773
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1074..1934)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="Bla"
                     /note="bla"
                     /db_xref="GI:403391486"
                     /protein_id="AFR43358.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1935..2039)
                     /gene="bla"
                     /note="AmpR promoter"
     mobile_element  2517..2714
                     /mobile_element_type="transposon:Tn7R"
                     /note="Tn7R"
     primer_bind     2719..2735
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2763..2857
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      2960..3046
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    3097..3144
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(3192..3725)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /note="GmR"
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3914..3942)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /note="Pc promoter"
                     /note="class 1 integron promoter"
     protein_bind    4059..4106
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(4107..4196)
                     /note="spy terminator"
     CDS             complement(4244..4921)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /note="mRFP1"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    4778..4797
                     /note="RR1"
     RBS             4928..4939
                     /note="RBS"
     RBS             4928..4939
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(4949..4983)
                     /note="J23119 (BglII) promoter"
     terminator      complement(5052..5102)
                     /note="L3S1P13 terminator"
     ncRNA           complement(5151..5243)
                     /note="optimized sgRNA"
                     /ncRNA_class="other"
     misc_feature    5244..5263
                     /note="RR1"
     promoter        complement(5270..5318)
                     /label=PLlacO1
                     /note="PLlacO-1 promoter"
     protein_bind    5276..5292
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5299..5313
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(5375..5431)
                     /note="thrLABC terminator"
     terminator      complement(5432..5485)
                     /note="garPLRK-rnpB terminator"
     protein_bind    5540..5561
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(5574..6656)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(6657..6734)
                     /gene="lacI (mutant)"
                     /note="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     terminator      6798..6850
                     /note="hisLGDCBHAFI terminator"
     terminator      6851..6899
                     /note="pdhR-aceEF-lpd terminator"
     promoter        7081..7129
                     /label=PLlacO1
                     /note="PLlacO-1 promoter"
     protein_bind    7086..7100
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    7107..7123
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             7165..11268
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /note="dCas9"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11271..11370
                     /codon_start=1
                     /note="3xMyc"
                     /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL"
     terminator      11402..11456
                     /note="L3S2P24 terminator"
     mobile_element  11523..11688
                     /mobile_element_type="transposon:Tn7"
                     /note="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
       61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
      121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
      181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
      241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa
      301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata
      361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt
      421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc
      481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa
      541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt
      601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga
      661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa
      721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg
      781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa
      841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt
     2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg
     2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct
     2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
     2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg
     2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg
     2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc
     3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta
     3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gatctcggct
     3181 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
     3241 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
     3301 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca
     3361 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
     3421 ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
     3481 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
     3541 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
     3601 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
     3661 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt
     3721 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     3781 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac
     3841 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     3901 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat
     3961 ccgtttccac ggtgtgcgtc catgggcaaa tattatacgc aaggcgacaa ggctcgagag
     4021 ttcaacagac agccttcgtt cttatggccg ttcaggagga agttcctatt ccgaagttcc
     4081 tattctctag aaagtatagg aacttcttga gaagagaaaa gaaaaccgcc gatcctgtcc
     4141 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaacga
     4201 atgacgaaaa ggctgtctga gcaaatccag gaggtcgttt ttattaagca ccggtggagt
     4261 gacgaccttc agcacgttcg tactgttcaa cgatggtgta gtcttcgttg tgggaggtga
     4321 tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg aaccggtttt ttagccatgt
     4381 aggtggtttt aacttcagcg tcgtagtgac caccgtcttt cagtttcaga cgcattttga
     4441 tttcaccttt cagagcaccg tcttccgggt acatacgttc ggtggaagct tcccaaccca
     4501 tggttttttt ctgcataacc ggaccgtcgg acgggaagtt ggtaccacgc agtttaactt
     4561 tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt aacggtaaca acaccaccgt
     4621 cttcgaagtt cataacacgt tcccatttga aaccttccgg gaaggacagt ttcaggtagt
     4681 ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc gtactggaac tgcggggaca
     4741 ggatgtccca agcgaacggc agcggaccac ctttggtaac tttcagttta gcggtctggg
     4801 taccttcgta cggacgacct tcaccttcac cttcgatttc gaactcgtga ccgttaacgg
     4861 aaccttccat acgaactttg aaacgcatga actctttgat aacgtcttcg ctactcgcca
     4921 tggtaccttt ctcctcttta attaattcag atctattata cctaggactg agctagctgt
     4981 caaattcacc accctgaatt gactctcaaa ccgtattagc ccggtatttt ggaaatagcg
     5041 gaagcactga cttttgttat caataaaaaa ggccccccgt tagggaggcc ttattgttcg
     5101 tctactcgga agagcgagag acaacagaac ggtcagccac atgaattcaa aaaaaaagca
     5161 ccgactcggt gccacttttt caagttgata acggactagc cttatttaaa cttgctatgc
     5221 tgtttccagc atagctctta aacagaccgc taaactgaaa gttactagaa gtatcttgtt
     5281 atccgctcac aatgtcaatg ttatccgctc acatttatag atctttagga attctttcag
     5341 ctcagtcgat aggtagtagg caagagtagt cgcacctttg gtcgaaaaaa aaagcccgca
     5401 ctgtcaggtg cgggcttttt tctgtgtttc cccaaaagta aaaacccgcc gaagcgggtt
     5461 tttacgtaaa acaggtgaaa ctgaccagac gagaaggctt tggaggtgat aatggggctc
     5521 aaggaccctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5581 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5641 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     5701 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     5761 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     5821 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     5881 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     5941 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     6001 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     6061 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     6121 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     6181 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     6241 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     6301 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     6361 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     6421 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     6481 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     6541 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     6601 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     6661 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac
     6721 cattcgatgg tgtcaacgta aatgcatgcc gcttgggagg cagataggta ggcatggccc
     6781 ccattttcaa tacaagcaac gcatgagaaa gcccccggaa gatcaccttc cgggggcttt
     6841 tttattgcgc tccggcaatt aaaaaagcgg ctaaccacgc cgcttttttt acgtctgcag
     6901 gcgaattgat ctggtttgac agcttatcac caagccagtt acctcggttc aaagagttgg
     6961 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
     7021 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt
     7081 ataaatgtga gcggataaca ttgacattgt gagcggataa caagatactg agcacatcag
     7141 caggtttcac acaggaaaac tagtatggat aagaaatact caataggctt agctatcggc
     7201 acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc
     7261 aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta
     7321 tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat
     7381 acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa
     7441 gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag
     7501 catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat
     7561 ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc
     7621 ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga
     7681 gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac
     7741 aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt
     7801 tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag
     7861 aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt
     7921 aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat
     7981 gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca
     8041 gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata
     8101 actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg
     8161 actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt
     8221 gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt
     8281 tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa
     8341 ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat
     8401 caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt
     8461 ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaatccc ttattatgtt
     8521 ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca
     8581 attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt
     8641 gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt
     8701 ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa
     8761 ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc
     8821 ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata
     8881 gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt
     8941 acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat
     9001 gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt
     9061 gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa
     9121 cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat
     9181 aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat
     9241 tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa
     9301 gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct
     9361 attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg
     9421 cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag
     9481 ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga
     9541 agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat
     9601 ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt
     9661 ttaagtgatt atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata
     9721 gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt
     9781 gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc
     9841 actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat
     9901 aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca
     9961 caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag
    10021 gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc
    10081 tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc
    10141 gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat
    10201 tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca
    10261 accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt
    10321 gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt
    10381 gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc
    10441 aatattgtca agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca
    10501 aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt
    10561 ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg
    10621 aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt
    10681 tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa
    10741 gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg
    10801 atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat
    10861 gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac
    10921 gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa
    10981 atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt
    11041 gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta
    11101 tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat
    11161 cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc
    11221 actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgacgc ggccgcggag
    11281 cagaaactca tctctgaaga agatctggaa caaaagttga tttcagaaga agatctggaa
    11341 cagaagctca tctctgagga agatctgtaa taaggcgcgc ctccttaatg ggacttgcag
    11401 cctcggtacc aaattccaga aaagacaccc gaaagggtgt tttttcgttt tggtcccaca
    11461 gaatgagcat catggctcta gtcgacatcg gagaagagta cggctctttt aaccgcctca
    11521 agaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag
    11581 cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa
    11641 aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt
    11701 tcttcctgtt atgtttgggc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    11761 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgacc cgctgcgctc ggtcgttcgg
    11821 ctgcggcgag cggtatcag
//
