LOCUS Exported 11839 bp ds-DNA circular SYN 18-OCT-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTn7C80_20160331 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11839) AUTHORS John Hawkins TITLE Direct Submission JOURNAL Exported Thursday, Oct 18, 2018 from SnapGene 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11839 /organism="synthetic DNA construct" /mol_type="other DNA" oriT 8..116 /note="incP origin of transfer" rep_origin 385..773 /note="R6K gamma ori" /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS complement(1074..1934) /codon_start=1 /transl_table=11 /gene="bla" /product="Bla" /note="bla" /db_xref="GI:403391486" /protein_id="AFR43358.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1935..2039) /gene="bla" /note="AmpR promoter" mobile_element 2517..2714 /mobile_element_type="transposon:Tn7R" /note="Tn7R" primer_bind 2719..2735 /note="KS primer" /note="common sequencing primer, one of multiple similar variants" terminator 2763..2857 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" terminator 2960..3046 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 3097..3144 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(3192..3725) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /note="GmR" /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3914..3942) /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" protein_bind 4059..4106 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." terminator complement(4107..4196) /note="spy terminator" CDS complement(4244..4921) /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /note="mRFP1" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" misc_feature 4778..4797 /note="RR1" RBS 4928..4939 /note="RBS" RBS 4928..4939 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" promoter complement(4949..4983) /note="J23119 (BglII) promoter" terminator complement(5052..5102) /note="L3S1P13 terminator" ncRNA complement(5151..5243) /note="optimized sgRNA" /ncRNA_class="other" misc_feature 5244..5263 /note="RR1" promoter complement(5270..5318) /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 5276..5292 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 5299..5313 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." terminator complement(5375..5431) /note="thrLABC terminator" terminator complement(5432..5485) /note="garPLRK-rnpB terminator" protein_bind 5540..5561 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." CDS complement(5574..6656) /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(6657..6734) /gene="lacI (mutant)" /note="lacIq promoter" /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." terminator 6798..6850 /note="hisLGDCBHAFI terminator" terminator 6851..6899 /note="pdhR-aceEF-lpd terminator" promoter 7081..7129 /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 7086..7100 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 7107..7123 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 7165..11268 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="Hsa Spy dCas9" /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10 mutation in the RuvC-like domain and the H840A mutation in the HNH domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11271..11370 /codon_start=1 /note="3xMyc" /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL" terminator 11402..11456 /note="L3S2P24 terminator" mobile_element 11523..11688 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag 61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct 121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc 181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg 241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa 301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata 361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt 421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc 481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa 541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt 601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga 661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa 721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg 781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa 841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt 2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg 2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct 2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac 2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg 2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg 2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc 3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta 3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gatctcggct 3181 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt 3241 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag 3301 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca 3361 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc 3421 ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg 3481 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc 3541 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca 3601 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt 3661 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt 3721 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt 3781 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac 3841 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg 3901 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat 3961 ccgtttccac ggtgtgcgtc catgggcaaa tattatacgc aaggcgacaa ggctcgagag 4021 ttcaacagac agccttcgtt cttatggccg ttcaggagga agttcctatt ccgaagttcc 4081 tattctctag aaagtatagg aacttcttga gaagagaaaa gaaaaccgcc gatcctgtcc 4141 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaacga 4201 atgacgaaaa ggctgtctga gcaaatccag gaggtcgttt ttattaagca ccggtggagt 4261 gacgaccttc agcacgttcg tactgttcaa cgatggtgta gtcttcgttg tgggaggtga 4321 tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg aaccggtttt ttagccatgt 4381 aggtggtttt aacttcagcg tcgtagtgac caccgtcttt cagtttcaga cgcattttga 4441 tttcaccttt cagagcaccg tcttccgggt acatacgttc ggtggaagct tcccaaccca 4501 tggttttttt ctgcataacc ggaccgtcgg acgggaagtt ggtaccacgc agtttaactt 4561 tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt aacggtaaca acaccaccgt 4621 cttcgaagtt cataacacgt tcccatttga aaccttccgg gaaggacagt ttcaggtagt 4681 ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc gtactggaac tgcggggaca 4741 ggatgtccca agcgaacggc agcggaccac ctttggtaac tttcagttta gcggtctggg 4801 taccttcgta cggacgacct tcaccttcac cttcgatttc gaactcgtga ccgttaacgg 4861 aaccttccat acgaactttg aaacgcatga actctttgat aacgtcttcg ctactcgcca 4921 tggtaccttt ctcctcttta attaattcag atctattata cctaggactg agctagctgt 4981 caaattcacc accctgaatt gactctcaaa ccgtattagc ccggtatttt ggaaatagcg 5041 gaagcactga cttttgttat caataaaaaa ggccccccgt tagggaggcc ttattgttcg 5101 tctactcgga agagcgagag acaacagaac ggtcagccac atgaattcaa aaaaaaagca 5161 ccgactcggt gccacttttt caagttgata acggactagc cttatttaaa cttgctatgc 5221 tgtttccagc atagctctta aacagaccgc taaactgaaa gttactagaa gtatcttgtt 5281 atccgctcac aatgtcaatg ttatccgctc acatttatag atctttagga attctttcag 5341 ctcagtcgat aggtagtagg caagagtagt cgcacctttg gtcgaaaaaa aaagcccgca 5401 ctgtcaggtg cgggcttttt tctgtgtttc cccaaaagta aaaacccgcc gaagcgggtt 5461 tttacgtaaa acaggtgaaa ctgaccagac gagaaggctt tggaggtgat aatggggctc 5521 aaggaccctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 5581 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 5641 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc 5701 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg 5761 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag 5821 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac 5881 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg 5941 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag 6001 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca 6061 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg 6121 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata 6181 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag 6241 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg 6301 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct 6361 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca 6421 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt 6481 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct 6541 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg 6601 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc 6661 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac 6721 cattcgatgg tgtcaacgta aatgcatgcc gcttgggagg cagataggta ggcatggccc 6781 ccattttcaa tacaagcaac gcatgagaaa gcccccggaa gatcaccttc cgggggcttt 6841 tttattgcgc tccggcaatt aaaaaagcgg ctaaccacgc cgcttttttt acgtctgcag 6901 gcgaattgat ctggtttgac agcttatcac caagccagtt acctcggttc aaagagttgg 6961 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag 7021 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt 7081 ataaatgtga gcggataaca ttgacattgt gagcggataa caagatactg agcacatcag 7141 caggtttcac acaggaaaac tagtatggac aagaagtaca gcatcggcct ggccatcggc 7201 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc 7261 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg cgccctgctg 7321 ttcgacagcg gagaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac 7381 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag 7441 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag 7501 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac 7561 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg 7621 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc 7681 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac 7741 aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg 7801 tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag 7861 aagaagaatg gcctgttcgg caacctgatt gccctgagcc tgggcctgac ccccaacttc 7921 aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac 7981 gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc 8041 gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc 8101 accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg 8161 accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc 8221 gaccagagca agaacggcta cgccggctac atcgatggcg gagccagcca ggaagagttc 8281 tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag 8341 ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac 8401 cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc 8461 ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg 8521 ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc 8581 atcaccccct ggaacttcga ggaagtggtg gacaagggcg ccagcgccca gagcttcatc 8641 gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc 8701 ctgctgtacg agtacttcac cgtgtacaac gagctgacca aagtgaaata cgtgaccgag 8761 ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aagccatcgt ggacctgctg 8821 ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc 8881 gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc 8941 acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac 9001 gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc 9061 gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag 9121 cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac 9181 aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac 9241 ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag 9301 gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc 9361 attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc 9421 cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag 9481 ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc 9541 agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac 9601 ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg 9661 ctgtccgact acgatgtgga cgctatcgtg cctcagagct ttctgaagga cgactccatc 9721 gataacaaag tgctgactcg gagcgacaag aaccggggca agagcgacaa cgtgccctcc 9781 gaagaggtcg tgaagaagat gaagaactac tggcgccagc tgctgaatgc caagctgatt 9841 acccagagga agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat 9901 aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca 9961 cagatcctgg actcccggat gaacactaag tacgacgaga acgacaaact gatccgggaa 10021 gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt 10081 tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc 10141 gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac 10201 tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct 10261 accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg 10321 gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac aggcgagatc 10381 gtgtgggata agggccggga ctttgccacc gtgcggaaag tgctgtctat gccccaagtg 10441 aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc 10501 aagaggaaca gcgacaagct gatcgccaga aagaaggact gggaccctaa gaagtacggc 10561 ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc 10621 aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc 10681 agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag 10741 gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga 10801 atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat 10861 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat 10921 gagcagaaac agctgtttgt ggaacagcac aaacactacc tggacgagat catcgagcag 10981 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa ggtgctgagc 11041 gcctacaaca agcacagaga caagcctatc agagagcagg ccgagaatat catccacctg 11101 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac 11161 cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc 11221 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacgc ggccgcggag 11281 cagaaactca tctctgaaga agatctggaa caaaagttga tttcagaaga agatctggaa 11341 cagaagctca tctctgagga agatctgtaa taaggcgcgc ctccttaatg ggacttgcag 11401 cctcggtacc aaattccaga aaagacaccc gaaagggtgt tttttcgttt tggtcccaca 11461 gaatgagcat catggctcta gtcgacatcg gagaagagta cggctctttt aaccgcctca 11521 agaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag 11581 cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa 11641 aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt 11701 tcttcctgtt atgtttgggc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 11761 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgacc cgctgcgctc ggtcgttcgg 11821 ctgcggcgag cggtatcag //