LOCUS Exported 11653 bp ds-DNA circular SYN 18-OCT-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTn7C81_20160331 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11653) AUTHORS John Hawkins TITLE Direct Submission JOURNAL Exported Thursday, Oct 18, 2018 from SnapGene 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11653 /organism="synthetic DNA construct" /mol_type="other DNA" oriT 8..116 /note="incP origin of transfer" rep_origin 385..773 /note="R6K gamma ori" /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS complement(1074..1934) /codon_start=1 /transl_table=11 /gene="bla" /product="Bla" /note="bla" /db_xref="GI:403391486" /protein_id="AFR43358.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1935..2039) /gene="bla" /note="AmpR promoter" mobile_element 2517..2714 /mobile_element_type="transposon:Tn7R" /note="Tn7R" primer_bind 2719..2735 /note="KS primer" /note="common sequencing primer, one of multiple similar variants" terminator 2763..2857 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" terminator 2960..3046 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 3097..3144 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(3192..3725) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /note="GmR" /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3914..3942) /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" protein_bind 4059..4106 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." terminator complement(4107..4196) /note="spy terminator" CDS complement(4244..4921) /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /note="mRFP1" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" misc_feature 4778..4797 /note="RR1" RBS 4928..4939 /note="RBS" RBS 4928..4939 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" promoter complement(4949..4983) /note="J23119 (BglII) promoter" terminator complement(5052..5102) /note="L3S1P13 terminator" terminator complement(5189..5245) /note="thrLABC terminator" terminator complement(5246..5299) /note="garPLRK-rnpB terminator" protein_bind 5354..5375 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." CDS complement(5388..6470) /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(6471..6548) /gene="lacI (mutant)" /note="lacIq promoter" /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." terminator 6612..6664 /note="hisLGDCBHAFI terminator" terminator 6665..6713 /note="pdhR-aceEF-lpd terminator" promoter 6895..6943 /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 6900..6914 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 6921..6937 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 6979..11082 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="Hsa Spy dCas9" /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10 mutation in the RuvC-like domain and the H840A mutation in the HNH domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11085..11184 /codon_start=1 /note="3xMyc" /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL" terminator 11216..11270 /note="L3S2P24 terminator" mobile_element 11337..11502 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag 61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct 121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc 181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg 241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa 301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata 361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt 421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc 481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa 541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt 601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga 661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa 721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg 781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa 841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt 2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg 2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct 2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac 2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg 2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg 2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc 3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta 3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gatctcggct 3181 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt 3241 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag 3301 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca 3361 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc 3421 ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg 3481 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc 3541 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca 3601 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt 3661 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt 3721 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt 3781 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac 3841 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg 3901 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat 3961 ccgtttccac ggtgtgcgtc catgggcaaa tattatacgc aaggcgacaa ggctcgagag 4021 ttcaacagac agccttcgtt cttatggccg ttcaggagga agttcctatt ccgaagttcc 4081 tattctctag aaagtatagg aacttcttga gaagagaaaa gaaaaccgcc gatcctgtcc 4141 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaacga 4201 atgacgaaaa ggctgtctga gcaaatccag gaggtcgttt ttattaagca ccggtggagt 4261 gacgaccttc agcacgttcg tactgttcaa cgatggtgta gtcttcgttg tgggaggtga 4321 tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg aaccggtttt ttagccatgt 4381 aggtggtttt aacttcagcg tcgtagtgac caccgtcttt cagtttcaga cgcattttga 4441 tttcaccttt cagagcaccg tcttccgggt acatacgttc ggtggaagct tcccaaccca 4501 tggttttttt ctgcataacc ggaccgtcgg acgggaagtt ggtaccacgc agtttaactt 4561 tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt aacggtaaca acaccaccgt 4621 cttcgaagtt cataacacgt tcccatttga aaccttccgg gaaggacagt ttcaggtagt 4681 ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc gtactggaac tgcggggaca 4741 ggatgtccca agcgaacggc agcggaccac ctttggtaac tttcagttta gcggtctggg 4801 taccttcgta cggacgacct tcaccttcac cttcgatttc gaactcgtga ccgttaacgg 4861 aaccttccat acgaactttg aaacgcatga actctttgat aacgtcttcg ctactcgcca 4921 tggtaccttt ctcctcttta attaattcag atctattata cctaggactg agctagctgt 4981 caaattcacc accctgaatt gactctcaaa ccgtattagc ccggtatttt ggaaatagcg 5041 gaagcactga cttttgttat caataaaaaa ggccccccgt tagggaggcc ttattgttcg 5101 tctactcgga agagcgagag acaacagaac ggtcagccac atgaattctt tcagctcagt 5161 cgataggtag taggcaagag tagtcgcacc tttggtcgaa aaaaaaagcc cgcactgtca 5221 ggtgcgggct tttttctgtg tttccccaaa agtaaaaacc cgccgaagcg ggtttttacg 5281 taaaacaggt gaaactgacc agacgagaag gctttggagg tgataatggg gctcaaggac 5341 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 5401 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 5461 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 5521 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 5581 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 5641 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 5701 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 5761 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 5821 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 5881 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 5941 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 6001 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 6061 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 6121 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 6181 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 6241 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 6301 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 6361 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 6421 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 6481 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcaccattcg 6541 atggtgtcaa cgtaaatgca tgccgcttgg gaggcagata ggtaggcatg gcccccattt 6601 tcaatacaag caacgcatga gaaagccccc ggaagatcac cttccggggg cttttttatt 6661 gcgctccggc aattaaaaaa gcggctaacc acgccgcttt ttttacgtct gcaggcgaat 6721 tgatctggtt tgacagctta tcaccaagcc agttacctcg gttcaaagag ttggtagctc 6781 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta 6841 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tattataaat 6901 gtgagcggat aacattgaca ttgtgagcgg ataacaagat actgagcaca tcagcaggtt 6961 tcacacagga aaactagtat ggacaagaag tacagcatcg gcctggccat cggcaccaac 7021 tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg 7081 ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggcgccct gctgttcgac 7141 agcggagaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga 7201 cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac 7261 gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag 7321 cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc 7381 atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc 7441 tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg 7501 aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag 7561 ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc 7621 agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag 7681 aatggcctgt tcggcaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc 7741 aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac 7801 ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag 7861 aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag 7921 gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg 7981 ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag 8041 agcaagaacg gctacgccgg ctacatcgat ggcggagcca gccaggaaga gttctacaag 8101 ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac 8161 agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc 8221 cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag 8281 gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct 8341 ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc 8401 ccctggaact tcgaggaagt ggtggacaag ggcgccagcg cccagagctt catcgagcgg 8461 atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg 8521 tacgagtact tcaccgtgta caacgagctg accaaagtga aatacgtgac cgagggaatg 8581 agaaagcccg ccttcctgag cggcgagcag aaaaaagcca tcgtggacct gctgttcaag 8641 accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc 8701 ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac 8761 cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac 8821 attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa 8881 cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg 8941 agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag 9001 tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg 9061 cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc 9121 ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag 9181 aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac 9241 aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag 9301 aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag 9361 atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac 9421 tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc 9481 gactacgatg tggacgctat cgtgcctcag agctttctga aggacgactc catcgataac 9541 aaagtgctga ctcggagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag 9601 gtcgtgaaga agatgaagaa ctactggcgc cagctgctga atgccaagct gattacccag 9661 aggaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc 9721 ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc 9781 ctggactccc ggatgaacac taagtacgac gagaacgaca aactgatccg ggaagtgaaa 9841 gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa 9901 gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga 9961 accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag 10021 gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc 10081 aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac 10141 ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaacaggcga gatcgtgtgg 10201 gataagggcc gggactttgc caccgtgcgg aaagtgctgt ctatgcccca agtgaatatc 10261 gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg 10321 aacagcgaca agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc 10381 gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc 10441 aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc 10501 gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg 10561 atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg 10621 gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac 10681 ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag 10741 aaacagctgt ttgtggaaca gcacaaacac tacctggacg agatcatcga gcagatcagc 10801 gagttctcca agagagtgat cctggccgac gctaatctgg acaaggtgct gagcgcctac 10861 aacaagcaca gagacaagcc tatcagagag caggccgaga atatcatcca cctgtttacc 10921 ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag 10981 aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc 11041 ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acgcggccgc ggagcagaaa 11101 ctcatctctg aagaagatct ggaacaaaag ttgatttcag aagaagatct ggaacagaag 11161 ctcatctctg aggaagatct gtaataaggc gcgcctcctt aatgggactt gcagcctcgg 11221 taccaaattc cagaaaagac acccgaaagg gtgttttttc gttttggtcc cacagaatga 11281 gcatcatggc tctagtcgac atcggagaag agtacggctc ttttaaccgc ctcaagaacc 11341 agataagtga aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg 11401 acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc 11461 atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc 11521 tgttatgttt gggcgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 11581 attgggcgct cttccgcttc ctcgctcact gacccgctgc gctcggtcgt tcggctgcgg 11641 cgagcggtat cag //