LOCUS Exported 11801 bp ds-DNA circular SYN 19-OCT-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTn7C92 SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 11801) AUTHORS John Hawkins TITLE Direct Submission JOURNAL Exported Friday, Oct 19, 2018 from SnapGene 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11801 /organism="recombinant plasmid" /mol_type="other DNA" oriT 8..116 /note="incP origin of transfer" rep_origin 385..773 /note="R6K gamma ori" /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS complement(1074..1934) /codon_start=1 /transl_table=11 /gene="bla" /product="Bla" /note="bla" /db_xref="GI:403391486" /protein_id="AFR43358.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1935..2039) /gene="bla" /note="AmpR promoter" mobile_element 2517..2714 /mobile_element_type="transposon:Tn7R" /note="Tn7R" primer_bind 2719..2735 /note="KS primer" /note="common sequencing primer, one of multiple similar variants" terminator 2763..2857 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" terminator 2960..3046 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 3097..3144 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(3196..3990) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(4101..4129) /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" protein_bind 4207..4254 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." terminator complement(4255..4344) /note="spy terminator" CDS complement(4392..5069) /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /note="mRFP1" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" misc_feature 4926..4945 /note="RR1" RBS 5076..5087 /note="RBS" RBS 5076..5087 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" promoter complement(5097..5131) /note="J23119 (BglII) promoter" terminator complement(5200..5250) /note="L3S1P13 terminator" terminator complement(5337..5393) /note="thrLABC terminator" terminator complement(5394..5447) /note="garPLRK-rnpB terminator" protein_bind 5502..5523 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." CDS complement(5536..6618) /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(6619..6696) /gene="lacI (mutant)" /note="lacIq promoter" /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." terminator 6760..6812 /note="hisLGDCBHAFI terminator" terminator 6813..6861 /note="pdhR-aceEF-lpd terminator" promoter 7043..7091 /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 7048..7062 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 7069..7085 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 7127..11230 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="Hsa Spy dCas9" /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10 mutation in the RuvC-like domain and the H840A mutation in the HNH domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11233..11332 /codon_start=1 /note="3xMyc" /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL" terminator 11364..11418 /note="L3S2P24 terminator" mobile_element 11485..11650 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag 61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct 121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc 181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg 241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa 301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata 361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt 421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc 481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa 541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt 601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga 661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa 721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg 781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa 841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt 2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg 2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct 2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac 2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg 2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg 2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc 3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta 3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gtcatttcga 3181 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga 3241 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc 3301 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg 3361 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc 3421 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa 3481 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc 3541 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca 3601 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc 3661 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca 3721 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc 3781 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt 3841 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca 3901 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga 3961 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc 4021 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agttttgagc 4081 gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg 4141 ccttcatccg tttccacggt ctcgagagtt caacagacag ccttcgttct tatggccgtt 4201 caggaggaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcttgaga 4261 agagaaaaga aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg 4321 gcggtcttaa gttttttggc tgaaacgaat gacgaaaagg ctgtctgagc aaatccagga 4381 ggtcgttttt attaagcacc ggtggagtga cgaccttcag cacgttcgta ctgttcaacg 4441 atggtgtagt cttcgttgtg ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc 4501 ggcagctgaa ccggtttttt agccatgtag gtggttttaa cttcagcgtc gtagtgacca 4561 ccgtctttca gtttcagacg cattttgatt tcacctttca gagcaccgtc ttccgggtac 4621 atacgttcgg tggaagcttc ccaacccatg gtttttttct gcataaccgg accgtcggac 4681 gggaagttgg taccacgcag tttaactttg tagatgaact caccgtcttg cagggaggag 4741 tcctgggtaa cggtaacaac accaccgtct tcgaagttca taacacgttc ccatttgaaa 4801 ccttccggga aggacagttt caggtagtcc gggatgtcag ccgggtgttt aacgtaagct 4861 ttggaaccgt actggaactg cggggacagg atgtcccaag cgaacggcag cggaccacct 4921 ttggtaactt tcagtttagc ggtctgggta ccttcgtacg gacgaccttc accttcacct 4981 tcgatttcga actcgtgacc gttaacggaa ccttccatac gaactttgaa acgcatgaac 5041 tctttgataa cgtcttcgct actcgccatg gtacctttct cctctttaat taattcagat 5101 ctattatacc taggactgag ctagctgtca aattcaccac cctgaattga ctctcaaacc 5161 gtattagccc ggtattttgg aaatagcgga agcactgact tttgttatca ataaaaaagg 5221 ccccccgtta gggaggcctt attgttcgtc tactcggaag agcgagagac aacagaacgg 5281 tcagccacat gaattctttc agctcagtcg ataggtagta ggcaagagta gtcgcacctt 5341 tggtcgaaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tccccaaaag 5401 taaaaacccg ccgaagcggg tttttacgta aaacaggtga aactgaccag acgagaaggc 5461 tttggaggtg ataatggggc tcaaggaccc tggggtgcct aatgagtgag ctaactcaca 5521 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 5581 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt 5641 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag 5701 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt 5761 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc 5821 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc 5881 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg 5941 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg 6001 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc 6061 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt 6121 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa 6181 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg 6241 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca 6301 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc 6361 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc 6421 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta 6481 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc 6541 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta 6601 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc 6661 cataccgcga aaggttttgc accattcgat ggtgtcaacg taaatgcatg ccgcttggga 6721 ggcagatagg taggcatggc ccccattttc aatacaagca acgcatgaga aagcccccgg 6781 aagatcacct tccgggggct tttttattgc gctccggcaa ttaaaaaagc ggctaaccac 6841 gccgcttttt ttacgtctgc aggcgaattg atctggtttg acagcttatc accaagccag 6901 ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg 6961 gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat 7021 cttattaatc agataaaata ttataaatgt gagcggataa cattgacatt gtgagcggat 7081 aacaagatac tgagcacatc agcaggtttc acacaggaaa actagtatgg acaagaagta 7141 cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta 7201 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa 7261 gaacctgatc ggcgccctgc tgttcgacag cggagaaaca gccgaggcca cccggctgaa 7321 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat 7381 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt 7441 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga 7501 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag 7561 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg 7621 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt 7681 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg 7741 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct 7801 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga ttgccctgag 7861 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca 7921 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca 7981 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat 8041 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata 8101 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga 8161 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acatcgatgg 8221 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg 8281 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt 8341 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg 8401 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac 8461 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat 8521 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg 8581 cgccagcgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga 8641 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtaca acgagctgac 8701 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa 8761 aaaagccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa 8821 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga 8881 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga 8941 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact 9001 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga 9061 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa 9121 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc 9181 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa 9241 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc 9301 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga 9361 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag 9421 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga 9481 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca 9541 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga 9601 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg tgcctcagag 9661 ctttctgaag gacgactcca tcgataacaa agtgctgact cggagcgaca agaaccgggg 9721 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcgcca 9781 gctgctgaat gccaagctga ttacccagag gaagttcgac aatctgacca aggccgagag 9841 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg 9901 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga 9961 gaacgacaaa ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga 10021 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca 10081 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga 10141 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag 10201 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt 10261 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac 10321 aaacggcgaa acaggcgaga tcgtgtggga taagggccgg gactttgcca ccgtgcggaa 10381 agtgctgtct atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt 10441 cagcaaagag tctatcctgc ccaagaggaa cagcgacaag ctgatcgcca gaaagaagga 10501 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt 10561 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg 10621 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa 10681 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga 10741 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga 10801 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct 10861 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaaacacta 10921 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc 10981 taatctggac aaggtgctga gcgcctacaa caagcacaga gacaagccta tcagagagca 11041 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa 11101 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc 11161 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct 11221 gggaggcgac gcggccgcgg agcagaaact catctctgaa gaagatctgg aacaaaagtt 11281 gatttcagaa gaagatctgg aacagaagct catctctgag gaagatctgt aataaggcgc 11341 gcctccttaa tgggacttgc agcctcggta ccaaattcca gaaaagacac ccgaaagggt 11401 gttttttcgt tttggtccca cagaatgagc atcatggctc tagtcgacat cggagaagag 11461 tacggctctt ttaaccgcct caagaaccag ataagtgaaa tctagttcca aactattttg 11521 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac 11581 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg 11641 tccgcccaca gcggggcatt tttcttcctg ttatgtttgg gcgctgcatt aatgaatcgg 11701 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 11761 cccgctgcgc tcggtcgttc ggctgcggcg agcggtatca g //