LOCUS Exported 11861 bp ds-DNA circular SYN 19-OCT-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTn7C108 SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 11861) AUTHORS John Hawkins TITLE Direct Submission JOURNAL Exported Friday, Oct 19, 2018 from SnapGene 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11861 /organism="recombinant plasmid" /mol_type="other DNA" oriT 8..116 /note="incP origin of transfer" rep_origin 385..773 /note="R6K gamma ori" /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS complement(1074..1934) /codon_start=1 /transl_table=11 /gene="bla" /product="Bla" /note="bla" /db_xref="GI:403391486" /protein_id="AFR43358.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1935..2039) /gene="bla" /note="AmpR promoter" mobile_element 2517..2714 /mobile_element_type="transposon:Tn7R" /note="Tn7R" primer_bind 2719..2735 /note="KS primer" /note="common sequencing primer, one of multiple similar variants" terminator 2763..2857 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" terminator 2960..3046 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 3097..3144 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(3204..3893) /codon_start=1 /note="cat (CmR)" /note="cat (Chloramphenicol resistance determinant)" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQFLCMRPIRKPPL PARWPIH" promoter complement(3976..4004) /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" protein_bind 4081..4128 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." terminator complement(4129..4218) /note="spy terminator" CDS complement(4266..4943) /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /note="mRFP1" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" misc_feature 4800..4819 /note="RR1" RBS 4950..4961 /note="RBS" RBS 4950..4961 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" promoter complement(4971..5005) /note="J23119 (BglII) promoter" terminator complement(5074..5124) /note="L3S1P13 terminator" ncRNA complement(5173..5265) /note="optimized sgRNA" /ncRNA_class="other" misc_feature 5266..5285 /note="RR1" promoter complement(5292..5340) /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 5298..5314 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 5321..5335 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." terminator complement(5397..5453) /note="thrLABC terminator" terminator complement(5454..5507) /note="garPLRK-rnpB terminator" protein_bind 5562..5583 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." CDS complement(5596..6678) /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(6679..6756) /gene="lacI (mutant)" /note="lacIq promoter" /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." terminator 6820..6872 /note="hisLGDCBHAFI terminator" terminator 6873..6921 /note="pdhR-aceEF-lpd terminator" promoter 7103..7151 /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 7108..7122 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 7129..7145 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 7187..11290 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="dCas9" /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11293..11392 /codon_start=1 /note="3xMyc" /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL" terminator 11424..11478 /note="L3S2P24 terminator" mobile_element 11545..11710 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag 61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct 121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc 181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg 241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa 301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata 361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt 421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc 481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa 541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt 601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga 661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa 721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg 781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa 841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt 2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg 2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct 2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac 2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg 2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg 2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc 3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta 3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gttccagtcg 3181 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 3241 cgtattgggc gcatgcataa aaactgttgt aattcattaa gcattctgcc gacatggaag 3301 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 3361 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 3421 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 3481 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 3541 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 3601 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 3661 ttcattgcca tacggaactc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 3721 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 3781 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 3841 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 3901 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tgagcgcata 3961 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc 4021 atccgtttcc acggctcgag agttcaacag acagccttcg ttcttatggc cgttcaggag 4081 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttctt gagaagagaa 4141 aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc 4201 ttaagttttt tggctgaaac gaatgacgaa aaggctgtct gagcaaatcc aggaggtcgt 4261 ttttattaag caccggtgga gtgacgacct tcagcacgtt cgtactgttc aacgatggtg 4321 tagtcttcgt tgtgggaggt gatgtccagt ttgatgtcgg ttttgtaagc acccggcagc 4381 tgaaccggtt ttttagccat gtaggtggtt ttaacttcag cgtcgtagtg accaccgtct 4441 ttcagtttca gacgcatttt gatttcacct ttcagagcac cgtcttccgg gtacatacgt 4501 tcggtggaag cttcccaacc catggttttt ttctgcataa ccggaccgtc ggacgggaag 4561 ttggtaccac gcagtttaac tttgtagatg aactcaccgt cttgcaggga ggagtcctgg 4621 gtaacggtaa caacaccacc gtcttcgaag ttcataacac gttcccattt gaaaccttcc 4681 gggaaggaca gtttcaggta gtccgggatg tcagccgggt gtttaacgta agctttggaa 4741 ccgtactgga actgcgggga caggatgtcc caagcgaacg gcagcggacc acctttggta 4801 actttcagtt tagcggtctg ggtaccttcg tacggacgac cttcaccttc accttcgatt 4861 tcgaactcgt gaccgttaac ggaaccttcc atacgaactt tgaaacgcat gaactctttg 4921 ataacgtctt cgctactcgc catggtacct ttctcctctt taattaattc agatctatta 4981 tacctaggac tgagctagct gtcaaattca ccaccctgaa ttgactctca aaccgtatta 5041 gcccggtatt ttggaaatag cggaagcact gacttttgtt atcaataaaa aaggcccccc 5101 gttagggagg ccttattgtt cgtctactcg gaagagcgag agacaacaga acggtcagcc 5161 acatgaattc aaaaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta 5221 gccttattta aacttgctat gctgtttcca gcatagctct taaacagacc gctaaactga 5281 aagttactag aagtatcttg ttatccgctc acaatgtcaa tgttatccgc tcacatttat 5341 agatctttag gaattctttc agctcagtcg ataggtagta ggcaagagta gtcgcacctt 5401 tggtcgaaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tccccaaaag 5461 taaaaacccg ccgaagcggg tttttacgta aaacaggtga aactgaccag acgagaaggc 5521 tttggaggtg ataatggggc tcaaggaccc tggggtgcct aatgagtgag ctaactcaca 5581 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 5641 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt 5701 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag 5761 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt 5821 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc 5881 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc 5941 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg 6001 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg 6061 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc 6121 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt 6181 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa 6241 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg 6301 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca 6361 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc 6421 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc 6481 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta 6541 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc 6601 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta 6661 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc 6721 cataccgcga aaggttttgc accattcgat ggtgtcaacg taaatgcatg ccgcttggga 6781 ggcagatagg taggcatggc ccccattttc aatacaagca acgcatgaga aagcccccgg 6841 aagatcacct tccgggggct tttttattgc gctccggcaa ttaaaaaagc ggctaaccac 6901 gccgcttttt ttacgtctgc aggcgaattg atctggtttg acagcttatc accaagccag 6961 ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg 7021 gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat 7081 cttattaatc agataaaata ttataaatgt gagcggataa cattgacatt gtgagcggat 7141 aacaagatac tgagcacatc agcaggtttc acacaggaaa actagtatgg ataagaaata 7201 ctcaataggc ttagctatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata 7261 taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa 7321 aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtctcaa 7381 acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat 7441 tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt 7501 tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga 7561 agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc 7621 tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg 7681 tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg acaaactatt 7741 tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta acgcaagtgg 7801 agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat tagaaaatct 7861 cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca ttgctttgtc 7921 attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca 7981 gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa ttggagatca 8041 atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac tttcagatat 8101 cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga ttaaacgcta 8161 cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac aacttccaga 8221 aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg 8281 gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa aaatggatgg 8341 tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc aacggacctt 8401 tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta ttttgagaag 8461 acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa aaatcttgac 8521 ttttcgaatc ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat 8581 gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg tcgataaagg 8641 tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc ttccaaatga 8701 aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata acgaattgac 8761 aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag gtgaacagaa 8821 gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa 8881 agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag gagttgaaga 8941 tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta aagataaaga 9001 ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa cattgacctt 9061 atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc tctttgatga 9121 taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa 9181 attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt ttttgaaatc 9241 agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt tgacatttaa 9301 agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg aacatattgc 9361 aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa aagttgttga 9421 tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg aaatggcacg 9481 tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga aacgaatcga 9541 agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg aaaatactca 9601 attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca tgtatgtgga 9661 ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatgccattg ttccacaaag 9721 tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata aaaatcgtgg 9781 taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact attggagaca 9841 acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga aagctgaacg 9901 tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg 9961 ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta aatacgatga 10021 aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat tagtttctga 10081 cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc atcatgccca 10141 tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc caaaacttga 10201 atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga ttgctaagtc 10261 tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata tcatgaactt 10321 cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc taatcgaaac 10381 taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca cagtgcgcaa 10441 agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga caggcggatt 10501 ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga 10561 ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt 10621 ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg 10681 gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt tagaagctaa 10741 aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata gtctttttga 10801 gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga 10861 gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt 10921 gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta 10981 tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc 11041 caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca 11101 agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg ctgcttttaa 11161 atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc 11221 cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct 11281 aggaggtgac gcggccgcgg agcagaaact catctctgaa gaagatctgg aacaaaagtt 11341 gatttcagaa gaagatctgg aacagaagct catctctgag gaagatctgt aataaggcgc 11401 gcctccttaa tgggacttgc agcctcggta ccaaattcca gaaaagacac ccgaaagggt 11461 gttttttcgt tttggtccca cagaatgagc atcatggctc tagtcgacat cggagaagag 11521 tacggctctt ttaaccgcct caagaaccag ataagtgaaa tctagttcca aactattttg 11581 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac 11641 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg 11701 tccgcccaca gcggggcatt tttcttcctg ttatgtttgg gcgctgcatt aatgaatcgg 11761 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 11821 cccgctgcgc tcggtcgttc ggctgcggcg agcggtatca g //