LOCUS Exported 10856 bp ds-DNA circular SYN 19-OCT-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTn7C118 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10856) AUTHORS John Hawkins TITLE Direct Submission JOURNAL Exported Friday, Oct 19, 2018 from SnapGene 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10856 /organism="synthetic DNA construct" /mol_type="other DNA" oriT 8..116 /note="incP origin of transfer" rep_origin 385..773 /note="R6K gamma ori" /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS complement(1074..1934) /codon_start=1 /transl_table=11 /gene="bla" /product="Bla" /note="bla" /db_xref="GI:403391486" /protein_id="AFR43358.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1935..2039) /gene="bla" /note="AmpR promoter" mobile_element 2517..2714 /mobile_element_type="transposon:Tn7R" /note="Tn7R" primer_bind 2719..2735 /note="KS primer" /note="common sequencing primer, one of multiple similar variants" terminator 2763..2857 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" terminator 2960..3046 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 3097..3144 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS complement(3192..3725) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /note="GmR" /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3914..3942) /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" protein_bind 4059..4106 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." terminator complement(4107..4196) /note="spy terminator" terminator complement(4285..4335) /note="L3S1P13 terminator" ncRNA complement(4384..4476) /note="optimized sgRNA" /ncRNA_class="other" promoter complement(4522..4580) /note="trc promoter" terminator complement(4627..4683) /note="thrLABC terminator" terminator complement(4684..4737) /note="garPLRK-rnpB terminator" terminator 4824..4876 /note="hisLGDCBHAFI terminator" terminator 4877..4925 /note="pdhR-aceEF-lpd terminator" CDS complement(4933..5811) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /note="araC" /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 5838..6122 /gene="araBAD" /note="araBAD promoter" /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" RBS 6149..6171 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 6183..10286 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="Hsa Spy dCas9" /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10 mutation in the RuvC-like domain and the H840A mutation in the HNH domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 10289..10388 /codon_start=1 /note="3xMyc" /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL" terminator 10420..10474 /note="L3S2P24 terminator" mobile_element 10540..10705 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag 61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct 121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc 181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg 241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa 301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata 361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt 421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc 481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa 541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt 601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga 661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa 721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg 781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa 841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt 2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg 2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct 2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac 2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg 2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg 2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc 3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta 3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gatctcggct 3181 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt 3241 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag 3301 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca 3361 atgccctgcc tccggtgctc tccggagact gcgagatcat agatatagat ctcactacgc 3421 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg 3481 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc 3541 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca 3601 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt 3661 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt 3721 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt 3781 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac 3841 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg 3901 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat 3961 ccgtttccac ggtgtgcgtc catgggcaaa tattatacgc aaggcgacaa ggctcgagag 4021 ttcaacagac agccttcgtt cttatggccg ttcaggagga agttcctatt ccgaagttcc 4081 tattctctag aaagtatagg aacttcttga gaagagaaaa gaaaaccgcc gatcctgtcc 4141 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaacga 4201 atgacgaaaa ggctgtctga gcaaatccag gaggtcgttt aaaccgtatt agcccggtat 4261 tttggaaata gcggaagcac tgacttttgt tatcaataaa aaaggccccc cgttagggag 4321 gccttattgt tcgtctactc ggaagagcga gagacaacag aacggtcagc cacatgaatt 4381 caaaaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt 4441 aaacttgcta tgctgtttcc agcatagctc ttaaacagag accgaccgct atggtggaga 4501 ccaaagttgg tctcaactag aattatacga gccggatgat taattgtcaa cagctcattt 4561 cagaatattt gccagaaccg gaattctttc agctcagtcg ataggtagta ggcaagagta 4621 gtcgcacctt tggtcgaaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt 4681 tccccaaaag taaaaacccg ccgaagcggg tttttacgta aaacaggtga aactgaccag 4741 acgagaaggc tttggaggtg ataatggggc tcaaggaccc gggaggcaga taggtaggca 4801 tggcccccat tttcaataca agcaacgcat gagaaagccc ccggaagatc accttccggg 4861 ggctttttta ttgcgctccg gcaattaaaa aagcggctaa ccacgccgct ttttttacgt 4921 ctgcagacta gtttatgaca acttgacggc tacatcattc actttttctt cacaaccggc 4981 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg 5041 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag 5101 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg 5161 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc 5221 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg 5281 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg 5341 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat 5401 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc 5461 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa 5521 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc 5581 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt 5641 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg 5701 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg 5761 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat 5821 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg 5881 cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct 5941 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg 6001 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt 6061 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc 6121 atacccgttt ttttgggcta gaaataattt tgtttaactt taagaaggag atatacatac 6181 ccatggacaa gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg 6241 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc 6301 ggcacagcat caagaagaac ctgatcggcg ccctgctgtt cgacagcgga gaaacagccg 6361 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct 6421 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca 6481 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg 6541 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa 6601 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc 6661 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg 6721 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc 6781 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca 6841 gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca 6901 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg 6961 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg 7021 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca 7081 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct 7141 ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc 7201 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg 7261 ccggctacat cgatggcgga gccagccagg aagagttcta caagttcatc aagcccatcc 7321 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc 7381 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc 7441 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga 7501 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca 7561 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg 7621 aagtggtgga caagggcgcc agcgcccaga gcttcatcga gcggatgacc aacttcgata 7681 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg 7741 tgtacaacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc 7801 tgagcggcga gcagaaaaaa gccatcgtgg acctgctgtt caagaccaac cggaaagtga 7861 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa 7921 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa 7981 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg 8041 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg 8101 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg 8161 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc 8221 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg 8281 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc 8341 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga 8401 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg 8461 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga 8521 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc 8581 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc 8641 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg 8701 ctatcgtgcc tcagagcttt ctgaaggacg actccatcga taacaaagtg ctgactcgga 8761 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga 8821 agaactactg gcgccagctg ctgaatgcca agctgattac ccagaggaag ttcgacaatc 8881 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac 8941 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga 9001 acactaagta cgacgagaac gacaaactga tccgggaagt gaaagtgatc accctgaagt 9061 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca 9121 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa 9181 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga 9241 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca 9301 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc 9361 ggcctctgat cgagacaaac ggcgaaacag gcgagatcgt gtgggataag ggccgggact 9421 ttgccaccgt gcggaaagtg ctgtctatgc cccaagtgaa tatcgtgaaa aagaccgagg 9481 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gacaagctga 9541 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg 9601 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg 9661 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg 9721 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta 9781 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac 9841 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca 9901 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg 9961 aacagcacaa acactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag 10021 tgatcctggc cgacgctaat ctggacaagg tgctgagcgc ctacaacaag cacagagaca 10081 agcctatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag 10141 cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca 10201 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga 10261 tcgacctgtc tcagctggga ggcgacgcgg ccgcggagca gaaactcatc tctgaagaag 10321 atctggaaca aaagttgatt tcagaagaag atctggaaca gaagctcatc tctgaggaag 10381 atctgtaata aggcgcgcct ccttaatggg acttgcagcc tcggtaccaa attccagaaa 10441 agacacccga aagggtgttt tttcgttttg gtcccacaga atgagcatca tggcctagtc 10501 gacatcggag aagagtacgg ctcttttaac cgcctcaaga accagataag tgaaatctag 10561 ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc 10621 catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag 10681 ttcccaacta ttttgtccgc ccacagcggg gcatttttct tcctgttatg tttgggcgct 10741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 10801 ttcctcgctc actgacccgc tgcgctcggt cgttcggctg cggcgagcgg tatcag //