LOCUS       Exported                4101 bp ds-DNA     circular SYN 19-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pTn7C136
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 19, 2018 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1967..4018
     rep_origin      1..546
                     /direction=RIGHT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        1072..1174
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1175..1834
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            1967..2986
                     /gene="glmS"
                     /locus_tag="BSU01780"
                     /note="glmS"
     CDS             1967..2986
                     /codon_start=1
                     /transl_table=11
                     /gene="glmS"
                     /locus_tag="BSU01780"
                     /product="L-glutamine-D-fructose-6-phosphate 
                     amidotransferase"
                     /function="2.2 : Metabolism of amino acids and related 
                     molecules"
                     /EC_number="2.6.1.16"
                     /note="'glmS"
                     /note="Evidence 2a : Function of homologous gene 
                     experimentally demonstrated in an other organism; PubMedId 
                     : 12682299, 15029187, 17114942, 17283212, 17322533; Product
                     type e : enzyme"
                     /inference="ab initio prediction:same species:2.0"
                     /translation="VMRKIIQTYQDENGKLSVPGDIAAAVAEADRIYIIGCGTSYHAGL
                     VGKQYIEMWANVPVEVHVASEFSYNMPLLSKKPLFIFLSQSGETADSRAVLVQVKALGH
                     KALTITNVPGSTLSREADYTLLLHAGPEIAVASTKAYTAQIAVLAVLASVAADKNGINI
                     GFDLVKELGIAANAMEALCDQKDEMEMIAREYLTVSRNAFFIGRGLDYFVCVEGALKLK
                     EISYIQAEGFAGGELKHGTIALIEQGTPVFALATQEHVNLSIRGNVKEVAARGANTCII
                     SLKGLDDADDRFVLPEVNPALAPLVSVVPLQLIAYYAALHRGCDVDKPRNLAKSVTVE"
     gene            complement(3454..4018)
                     /gene="alkA"
                     /locus_tag="BSU01800"
                     /note="alkA"
     CDS             complement(3454..4018)
                     /codon_start=2
                     /transl_table=11
                     /gene="alkA"
                     /locus_tag="BSU01800"
                     /product="DNA-3-methyladenine glycosylase"
                     /function="3.2 : DNA restriction/modification and repair"
                     /EC_number="3.2.2.21"
                     /note="'alkA"
                     /note="Evidence 1a : Function experimentally demonstrated 
                     in the studied strain; PubMedId : 7565865, 8376346; Product
                     type e : enzyme"
                     /inference="ab initio prediction:same species:2.0"
                     /translation="RKFYGLRVIGIPDLFEALCWGVLGQQINLAFAYSLKKQFVEAFGD
                     SIEWNGKKYWVFPPYERIARLTPTDLADIKMTVKKSEYIIGIARLMASGELSREKLMKM
                     NFKDAEKNLIKIRGIGPWTANYVLMRCLRFPTAFPIDDVGLIHSIKILRNMNRKPTKDE
                     ILEISVPWKEWQSYATFYLWRVLY"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
      721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc
      781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgct tttgccgtta
      841 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
      901 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccgacgcact
      961 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
     1021 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca
     1081 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     1141 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1201 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1261 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1321 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1381 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1441 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1501 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1561 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1621 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1681 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1741 tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca
     1801 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     1861 ttaaacgcct ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     1921 aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatgtta tgcgcaaaat
     1981 catccaaacg tatcaagatg aaaacggcaa gctgtctgtg cctggcgata tcgctgccgc
     2041 tgtagcggaa gcggaccgca tctatatcat tggctgcgga acaagctacc atgcaggact
     2101 tgtcggtaaa caatatattg aaatgtgggc aaacgtgccg gttgaagtgc atgtagcgag
     2161 tgaattctcc tacaacatgc cgcttctgtc taagaaaccg ctcttcattt tcctttctca
     2221 aagcggagaa acagcagaca gccgcgcggt actcgttcaa gtcaaagcgc tcggacacaa
     2281 agccctgaca atcacaaacg tacctggatc aacgctttct cgtgaagctg actatacatt
     2341 gctgcttcat gcaggccctg agatcgctgt tgcgtcaacg aaagcataca ctgcacaaat
     2401 cgcagttctg gcggttcttg cttctgtggc tgctgacaaa aatggcatca atatcggatt
     2461 tgacctcgtc aaagaactcg gtatcgctgc aaacgcaatg gaagctctat gcgaccagaa
     2521 agacgaaatg gaaatgatcg ctcgtgaata cctgactgta tccagaaatg ctttcttcat
     2581 cggacgcggc cttgactact tcgtatgtgt cgaaggcgca ctgaagctga aagagatttc
     2641 ttacatccag gcagaaggtt ttgccggcgg tgagctaaag cacggaacga ttgccttgat
     2701 cgaacaagga acaccagtat tcgcactggc aactcaagag catgtaaacc taagcatccg
     2761 cggaaacgtc aaagaagttg ctgctcgcgg agcaaacaca tgcatcatct cactgaaagg
     2821 cctagacgat gcggatgaca gattcgtatt gccggaagta aacccagcgc ttgctccgtt
     2881 ggtatctgtt gttccattgc agctgatcgc ttactatgct gcactgcatc gcggctgtga
     2941 tgtggataaa cctcgtaacc tggcaaaatc ggttacggtt gagtaataaa tgtttaaccc
     3001 ctttggataa gattatctaa aggggtgttt ttatgtccaa aagcgaaaga acgtctacag
     3061 tggaaaaatg gattaaagaa gttgatggta caggacccga ctatcgatcg accatggcta
     3121 tagatttaag atgtttcctc attagatcgg tcaatcaggt taaaaggcat taaaacttgg
     3181 agagaaactg cttttgccta actgagtttt ctaatgttaa tacagatata agagagcagt
     3241 tcctcattaa gatacgcttt tgatagcagt tggaatttag catgctagaa atccgaaaac
     3301 cggagagcct attggaatga taacgtgttt ttttattgac aattaataaa gggcaaagaa
     3361 ttctagaagt cgcacttaca atgtacttgt gggattaggt tttaaagttc attctttttt
     3421 tattgagtac attccggtta aagttgaaat gattcagtaa aggacacgcc ataaataaaa
     3481 ggtagcgtat gattgccatt ctttccatgg aaccgatatt tctaagattt catctttcgt
     3541 aggctttcgg ttcatattac gtaatatttt tatcgaatga ataagaccca catcatcgat
     3601 tggaaaagct gtcgggaacc taaggcagcg cattagaaca taattggctg tccaaggacc
     3661 gattcctcgt attttaatta agtttttttc agcatcttta aagttcattt tcattaattt
     3721 ttccctcgat aattctccac ttgccattaa tctggctatt ccaatgatat attcactttt
     3781 tttcaccgtc attttaatat ctgccaggtc ggtaggggtt aaccgtgcaa ttcgctcgta
     3841 cggtgggaac acccaatact ttttaccatt ccattcaata gaatcgccaa atgcttctac
     3901 aaattgcttc tttaaggagt acgcgaaggc taagttaatt tgttgcccta aaactcccca
     3961 acataaagct tcaaataaat cgggaatgcc gatcactcgc aacccataaa attttcgggg
     4021 gctgacttca ggtgctacat ttgaagagat aaattgcact gaaatctaga aatattttat
     4081 ctgattaata agatgatctt c
//
