LOCUS Exported 11249 bp ds-DNA circular SYN 19-OCT-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTn7C171 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11249) AUTHORS John Hawkins TITLE Direct Submission JOURNAL Exported Friday, Oct 19, 2018 from SnapGene 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11249 /organism="synthetic DNA construct" /mol_type="other DNA" mobile_element 152..317 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)" terminator complement(383..437) /note="L3S2P24 terminator" CDS complement(469..568) /codon_start=1 /note="3xMyc" /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL" CDS complement(571..4674) /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="Hsa Spy dCas9" /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10 mutation in the RuvC-like domain and the H840A mutation in the HNH domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" promoter complement(4710..4758) /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 4716..4732 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 4739..4753 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." terminator complement(4946..4994) /note="pdhR-aceEF-lpd terminator" terminator complement(4995..5047) /note="hisLGDCBHAFI terminator" promoter 5114..5191 /gene="lacI (mutant)" /note="lacIq promoter" /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." CDS 5192..6274 /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" terminator 6366..6419 /note="garPLRK-rnpB terminator" terminator 6420..6476 /note="thrLABC terminator" promoter 6533..6581 /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 6538..6552 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 6559..6575 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ncRNA 6627..6719 /note="optimized sgRNA" /ncRNA_class="other" terminator 6768..6818 /note="L3S1P13 terminator" terminator 6907..6996 /note="spy terminator" protein_bind complement(6997..7044) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." promoter 7121..7149 /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" CDS 7260..8054 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" protein_bind complement(8106..8153) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." terminator 8204..8290 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" terminator complement(8393..8487) /note="lambda t0 terminator" /note="transcription terminator from phage lambda" primer_bind complement(8515..8531) /note="KS primer" /note="common sequencing primer, one of multiple similar variants" mobile_element 8536..8733 /mobile_element_type="transposon:Tn7R" /note="Tn7R" promoter 9211..9315 /gene="bla" /note="AmpR promoter" CDS 9316..10176 /codon_start=1 /transl_table=11 /gene="bla" /product="Bla" /note="bla" /db_xref="GI:403391486" /protein_id="AFR43358.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 10477..10865 /note="R6K gamma ori" /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" oriT 11134..11242 /note="incP origin of transfer" ORIGIN 1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcgg gtcagtgagc gaggaagcgg 61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg 121 cccaaacata acaggaagaa aaatgccccg ctgtgggcgg acaaaatagt tgggaactgg 181 gaggggtgga aatggagttt ttaaggatta tttagggaag agtgacaaaa tagatgggaa 241 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag 301 atttcactta tctggttctt gaggcggtta aaagagccgt actcttctcc gatgtcgact 361 aggccatgat gctcattctg tgggaccaaa acgaaaaaac accctttcgg gtgtcttttc 421 tggaatttgg taccgaggct gcaagtccca ttaaggaggc gcgccttatt acagatcttc 481 ctcagagatg agcttctgtt ccagatcttc ttctgaaatc aacttttgtt ccagatcttc 541 ttcagagatg agtttctgct ccgcggccgc gtcgcctccc agctgagaca ggtcgatccg 601 tgtctcgtac aggccggtga tgctctggtg gatcagggtg gcgtccagca cctctttggt 661 gctggtgtac ctcttccggt cgatggtggt gtcaaagtac ttgaaggcgg caggggctcc 721 cagattggtc agggtaaaca ggtggatgat attctcggcc tgctctctga taggcttgtc 781 tctgtgcttg ttgtaggcgc tcagcacctt gtccagatta gcgtcggcca ggatcactct 841 cttggagaac tcgctgatct gctcgatgat ctcgtccagg tagtgtttgt gctgttccac 901 aaacagctgt ttctgctcat tatcctcggg ggagcccttc agcttctcat agtggctggc 961 caggtacagg aagttcacat atttggaggg cagggccagt tcgtttccct tctgcagttc 1021 gccggcagag gccagcattc tcttccggcc gttttccagc tcgaacaggg agtacttagg 1081 cagcttgatg atcaggtcct ttttcacttc tttgtagccc ttggcttcca gaaagtcgat 1141 gggattcttc tcgaagctgc ttctttccat gatggtgatc cccagcagct ctttcacact 1201 cttcagtttc ttggacttgc ccttttccac tttggccacc accagcacag aataggccac 1261 ggtggggctg tcgaagccgc cgtacttctt agggtcccag tccttctttc tggcgatcag 1321 cttgtcgctg ttcctcttgg gcaggataga ctctttgctg aagccgcctg tctgcacctc 1381 ggtctttttc acgatattca cttggggcat agacagcact ttccgcacgg tggcaaagtc 1441 ccggccctta tcccacacga tctcgcctgt ttcgccgttt gtctcgatca gaggccgctt 1501 ccggatctcg ccgttggcca gggtaatctc ggtcttgaaa aagttcatga tgttgctgta 1561 gaagaagtac ttggcggtag ccttgccgat ttcctgctcg ctcttggcga tcatcttccg 1621 cacgtcgtac accttgtagt cgccgtacac gaactcgctt tccagcttag ggtacttttt 1681 gatcagggcg gttcccacga cggcgttcag gtaggcgtcg tgggcgtggt ggtagttgtt 1741 gatctcgcgc actttgtaaa actggaaatc cttccggaaa tcggacacca gcttggactt 1801 cagggtgatc actttcactt cccggatcag tttgtcgttc tcgtcgtact tagtgttcat 1861 ccgggagtcc aggatctgtg ccacgtgctt tgtgatctgc cgggtttcca ccagctgtct 1921 cttgatgaag ccggccttat ccagttcgct caggccgcct ctctcggcct tggtcagatt 1981 gtcgaacttc ctctgggtaa tcagcttggc attcagcagc tggcgccagt agttcttcat 2041 cttcttcacg acctcttcgg agggcacgtt gtcgctcttg ccccggttct tgtcgctccg 2101 agtcagcact ttgttatcga tggagtcgtc cttcagaaag ctctgaggca cgatagcgtc 2161 cacatcgtag tcggacagcc ggttgatgtc cagttcctgg tccacgtaca tatcccgccc 2221 attctgcagg tagtacaggt acagcttctc gttctgcagc tgggtgtttt ccacggggtg 2281 ttctttcagg atctggctgc ccagctcttt gatgccctct tcgatccgct tcattctctc 2341 gcggctgttc ttctgtccct tctgggtggt ctggttctct ctggccattt cgatcacgat 2401 gttctcgggc ttgtgccggc ccatcacttt cacgagctcg tccaccacct tcactgtctg 2461 caggatgccc ttcttaatgg cggggctgcc ggccagattg gcaatgtgct cgtgcaggct 2521 atcgccctgg ccggacacct gggctttctg gatgtcctct ttaaaggtca ggctgtcgtc 2581 gtggatcagc tgcatgaagt ttctgttggc gaagccgtcg gacttcagga aatccaggat 2641 tgtcttgccg gactgcttgt cccggatgcc gttgatcagc ttccggctca gcctgcccca 2701 gccggtgtat ctccgccgct tcagctgctt catcactttg tcgtcgaaca ggtgggcata 2761 ggttttcagc cgttcctcga tcatctctct gtcctcaaac agtgtcaggg tcagcacgat 2821 atcttccaga atgtcctcgt tttcctcatt gtccaggaag tccttgtcct tgataatttt 2881 cagcagatcg tggtatgtgc ccagggaggc gttgaaccga tcttccacgc cggagatttc 2941 cacggagtcg aagcactcga ttttcttgaa gtagtcctct ttcagctgct tcacggtcac 3001 tttccggttg gtcttgaaca gcaggtccac gatggctttt ttctgctcgc cgctcaggaa 3061 ggcgggcttt ctcattccct cggtcacgta tttcactttg gtcagctcgt tgtacacggt 3121 gaagtactcg tacagcaggc tgtgcttggg cagcaccttc tcgttgggca ggttcttatc 3181 gaagttggtc atccgctcga tgaagctctg ggcgctggcg cccttgtcca ccacttcctc 3241 gaagttccag ggggtgatgg tttcctcgct ctttctggtc atccaggcga atctgctgtt 3301 tcccctggcc agagggccca cgtagtaggg gatgcggaag gtcaggatct tctcgatctt 3361 ttcccggttg tccttcagga atgggtaaaa atcttcctgc cgccgcagaa tggcgtgcag 3421 ctctcccagg tggatctggt gggggatgct gccgttgtcg aaggtccgct gcttccgcag 3481 caggtcctct ctgttcagct tcacgagcag ttcctcggtg ccgtccatct tttccaggat 3541 gggcttgatg aacttgtaga actcttcctg gctggctccg ccatcgatgt agccggcgta 3601 gccgttcttg ctctggtcga agaaaatctc tttgtacttc tcaggcagct gctgccgcac 3661 gagagctttc agcagggtca ggtcctggtg gtgctcgtcg tatctcttga tcatagaggc 3721 gctcaggggg gccttggtga tctcggtgtt cactctcagg atgtcgctca gcaggatggc 3781 gtcggacagg ttcttggcgg ccagaaacag gtcggcgtac tggtcgccga tctgggccag 3841 caggttgtcc aggtcgtcgt cgtaggtgtc cttgctcagc tgcagtttgg catcctcggc 3901 caggtcgaag ttgctcttga agttgggggt caggcccagg ctcagggcaa tcaggttgcc 3961 gaacaggcca ttcttcttct cgccgggcag ctgggcgatc agattttcca gccgtctgct 4021 cttgctcagt ctggcagaca ggatggcctt ggcgtccacg ccgctggcgt tgatggggtt 4081 ttcctcgaac agctggttgt aggtctgcac cagctggatg aacagcttgt ccacgtcgct 4141 gttgtcgggg ttcaggtcgc cctcgatcag gaagtggccc cggaacttga tcatgtgggc 4201 cagggccaga tagatcagcc gcaggtcggc cttgtcggtg ctgtccacca gtttctttct 4261 caggtggtag atggtggggt acttctcgtg gtaggccacc tcgtccacga tgttgccgaa 4321 gatggggtgc cgctcgtgct tcttatcctc ttccaccagg aaggactctt ccagtctgtg 4381 gaagaagctg tcgtccacct tggccatctc gttgctgaag atctcttgca gatagcagat 4441 ccggttcttc cgtctggtgt atcttcttct ggcggttctc ttcagccggg tggcctcggc 4501 tgtttctccg ctgtcgaaca gcagggcgcc gatcaggttc ttcttgatgc tgtgccggtc 4561 ggtgttgccc agcaccttga atttcttgct gggcaccttg tactcgtcgg tgatcacggc 4621 ccagcccaca gagttggtgc cgatggccag gccgatgctg tacttcttgt ccatactagt 4681 tttcctgtgt gaaacctgct gatgtgctca gtatcttgtt atccgctcac aatgtcaatg 4741 ttatccgctc acatttataa tattttatct gattaataag atgatcttct tgagatcgtt 4801 ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt 4861 cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttggt gataagctgt 4921 caaaccagat caattcgcac tagtctgcag acgtaaaaaa agcggcgtgg ttagccgctt 4981 ttttaattgc cggagcgcaa taaaaaagcc cccggaaggt gatcttccgg gggctttctc 5041 atgcgttgct tgtattgaaa atgggggcca tgcctaccta tctgcctccc gggaagcggc 5101 atgcatttac gttgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg 5161 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc 5221 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt 5281 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa 5341 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag 5401 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact 5461 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc 5521 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga 5581 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt 5641 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg 5701 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag 5761 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat 5821 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat 5881 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc 5941 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt 6001 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa 6061 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg 6121 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct 6181 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 6241 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc 6301 tcactcatta ggcaccccac ccgggtcctt gagccccatt atcacctcca aagccttctc 6361 gtctggtcag tttcacctgt tttacgtaaa aacccgcttc ggcgggtttt tacttttggg 6421 gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac caaaggtgcg 6481 actactcttg cctactacct atcgactgag ctgaaagaat tcctaaagat ctataaatgt 6541 gagcggataa cattgacatt gtgagcggat aacaagatac ttctagttga gaccaacttt 6601 ggtctccacc atagcggtcg gtctctgttt aagagctatg ctggaaacag catagcaagt 6661 ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttt 6721 tgaattcatg tggctgaccg ttctgttgtc tctcgctctt ccgagtagac gaacaataag 6781 gcctccctaa cggggggcct tttttattga taacaaaagt cagtgcttcc gctatttcca 6841 aaataccggg ctaatacggt ttaaacgacc tcctggattt gctcagacag ccttttcgtc 6901 attcgtttca gccaaaaaac ttaagaccgc cggtcttgtc cactaccttg cagtaatgcg 6961 gtggacagga tcggcggttt tcttttctct tctcaagaag ttcctatact ttctagagaa 7021 taggaacttc ggaataggaa cttcctcctg aacggccata agaacgaagg ctgtctgttg 7081 aactctcgag ccgtggaaac ggatgaaggc acgaacccag ttgacataag cctgttcggt 7141 tcgtaaactg taatgcaagt agcgtatgcg ctcaaaactg gatggctttc ttgccgccaa 7201 ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca 7261 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 7321 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 7381 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 7441 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 7501 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 7561 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 7621 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 7681 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 7741 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 7801 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 7861 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 7921 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 7981 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 8041 acgagttctt ctgagcggga ctctggggtt cgaaatgact cgagcatcag cttcaaaagc 8101 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggtac ttcaagatcc 8161 ccaattcgag ctcatcgtcc gggccgcaag ctcctagcgg cggatttgtc ctactcagga 8221 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc 8281 gttttatttg atgcctcaag ctagagagtc attaccccag gcgtttaagg gcaccaataa 8341 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtctagctt ggattctcac 8401 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg 8461 tcattactgg atctatcaac aggagtccaa gctcagctaa ttaagctagc ttatcgatac 8521 cgtcgacctc gaaccccacg cccctcttta atacgacggg caatttgcac ttcagaaaat 8581 gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact 8641 gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat 8701 tttgttcagt ttaagacttt attgtccgcc cacacccgct tacgcagggc atccatttat 8761 tactcaaccg taaccgattt tgccaggtta cgcggctggt cctctagctg gcgtaatagc 8821 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 8881 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact 8941 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 9001 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 9061 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga 9121 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 9181 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 9241 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 9301 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 9361 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 9421 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 9481 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 9541 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 9601 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 9661 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 9721 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 9781 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 9841 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 9901 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 9961 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 10021 ggtgagcgtg ggtcccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 10081 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 10141 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 10201 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 10261 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 10321 cccgtagaaa agatcaaagg cagttattgg tgcctcactg attaagcatt ggtaactgtc 10381 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttttgcg 10441 gccgcaagat ccggccacga tgcgtccggc gtagaggatc tgaagatcag cagttcaacc 10501 tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg tttaacgtac 10561 taagctctca tgtttaacga actaaaccct catggctaac gtactaagct ctcatggcta 10621 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata 10681 taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata 10741 taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc 10801 actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac acttaacggc 10861 tgacatggga attagccatg gcatcacagt atcgtgatga cagaggcagg gagtgcggcc 10921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatca 10981 ggggattcct taaggtatac tttccgctgc ataaccctgc ttcggggtca ttatagcgat 11041 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt 11101 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc 11161 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa 11221 tcctgctctg cgaggctggc cgataagct //