LOCUS Exported 11230 bp ds-DNA circular SYN 21-OCT-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTn7C171 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11230) AUTHORS John Hawkins TITLE Direct Submission JOURNAL Exported Sunday, Oct 21, 2018 from SnapGene 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11230 /organism="synthetic DNA construct" /mol_type="other DNA" mobile_element 152..317 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)" terminator complement(383..437) /note="L3S2P24 terminator" CDS complement(469..568) /codon_start=1 /note="3xMyc" /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL" CDS complement(571..4674) /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="Hsa Spy dCas9" /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10 mutation in the RuvC-like domain and the H840A mutation in the HNH domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" promoter complement(4710..4758) /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 4716..4732 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 4739..4753 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." terminator complement(4946..4994) /note="pdhR-aceEF-lpd terminator" terminator complement(4995..5047) /note="hisLGDCBHAFI terminator" promoter 5114..5191 /gene="lacI (mutant)" /note="lacIq promoter" /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." CDS 5192..6274 /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" terminator 6366..6419 /note="garPLRK-rnpB terminator" terminator 6420..6476 /note="thrLABC terminator" promoter 6533..6581 /label=PLlacO1 /note="PLlacO-1 promoter" protein_bind 6538..6552 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 6559..6575 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 6588..6607 /note="folA" ncRNA 6608..6700 /note="optimized sgRNA" /ncRNA_class="other" terminator 6749..6799 /note="L3S1P13 terminator" terminator 6888..6977 /note="spy terminator" protein_bind complement(6978..7025) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." promoter 7102..7130 /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" CDS 7241..8035 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" protein_bind complement(8087..8134) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." terminator 8185..8271 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" terminator complement(8374..8468) /note="lambda t0 terminator" /note="transcription terminator from phage lambda" primer_bind complement(8496..8512) /note="KS primer" /note="common sequencing primer, one of multiple similar variants" mobile_element 8517..8714 /mobile_element_type="transposon:Tn7R" /note="Tn7R" promoter 9192..9296 /gene="bla" /note="AmpR promoter" CDS 9297..10157 /codon_start=1 /transl_table=11 /gene="bla" /product="Bla" /note="bla" /db_xref="GI:403391486" /protein_id="AFR43358.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 10458..10846 /note="R6K gamma ori" /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" oriT 11115..11223 /note="incP origin of transfer" ORIGIN 1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcgg gtcagtgagc gaggaagcgg 61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg 121 cccaaacata acaggaagaa aaatgccccg ctgtgggcgg acaaaatagt tgggaactgg 181 gaggggtgga aatggagttt ttaaggatta tttagggaag agtgacaaaa tagatgggaa 241 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag 301 atttcactta tctggttctt gaggcggtta aaagagccgt actcttctcc gatgtcgact 361 aggccatgat gctcattctg tgggaccaaa acgaaaaaac accctttcgg gtgtcttttc 421 tggaatttgg taccgaggct gcaagtccca ttaaggaggc gcgccttatt acagatcttc 481 ctcagagatg agcttctgtt ccagatcttc ttctgaaatc aacttttgtt ccagatcttc 541 ttcagagatg agtttctgct ccgcggccgc gtcgcctccc agctgagaca ggtcgatccg 601 tgtctcgtac aggccggtga tgctctggtg gatcagggtg gcgtccagca cctctttggt 661 gctggtgtac ctcttccggt cgatggtggt gtcaaagtac ttgaaggcgg caggggctcc 721 cagattggtc agggtaaaca ggtggatgat attctcggcc tgctctctga taggcttgtc 781 tctgtgcttg ttgtaggcgc tcagcacctt gtccagatta gcgtcggcca ggatcactct 841 cttggagaac tcgctgatct gctcgatgat ctcgtccagg tagtgtttgt gctgttccac 901 aaacagctgt ttctgctcat tatcctcggg ggagcccttc agcttctcat agtggctggc 961 caggtacagg aagttcacat atttggaggg cagggccagt tcgtttccct tctgcagttc 1021 gccggcagag gccagcattc tcttccggcc gttttccagc tcgaacaggg agtacttagg 1081 cagcttgatg atcaggtcct ttttcacttc tttgtagccc ttggcttcca gaaagtcgat 1141 gggattcttc tcgaagctgc ttctttccat gatggtgatc cccagcagct ctttcacact 1201 cttcagtttc ttggacttgc ccttttccac tttggccacc accagcacag aataggccac 1261 ggtggggctg tcgaagccgc cgtacttctt agggtcccag tccttctttc tggcgatcag 1321 cttgtcgctg ttcctcttgg gcaggataga ctctttgctg aagccgcctg tctgcacctc 1381 ggtctttttc acgatattca cttggggcat agacagcact ttccgcacgg tggcaaagtc 1441 ccggccctta tcccacacga tctcgcctgt ttcgccgttt gtctcgatca gaggccgctt 1501 ccggatctcg ccgttggcca gggtaatctc ggtcttgaaa aagttcatga tgttgctgta 1561 gaagaagtac ttggcggtag ccttgccgat ttcctgctcg ctcttggcga tcatcttccg 1621 cacgtcgtac accttgtagt cgccgtacac gaactcgctt tccagcttag ggtacttttt 1681 gatcagggcg gttcccacga cggcgttcag gtaggcgtcg tgggcgtggt ggtagttgtt 1741 gatctcgcgc actttgtaaa actggaaatc cttccggaaa tcggacacca gcttggactt 1801 cagggtgatc actttcactt cccggatcag tttgtcgttc tcgtcgtact tagtgttcat 1861 ccgggagtcc aggatctgtg ccacgtgctt tgtgatctgc cgggtttcca ccagctgtct 1921 cttgatgaag ccggccttat ccagttcgct caggccgcct ctctcggcct tggtcagatt 1981 gtcgaacttc ctctgggtaa tcagcttggc attcagcagc tggcgccagt agttcttcat 2041 cttcttcacg acctcttcgg agggcacgtt gtcgctcttg ccccggttct tgtcgctccg 2101 agtcagcact ttgttatcga tggagtcgtc cttcagaaag ctctgaggca cgatagcgtc 2161 cacatcgtag tcggacagcc ggttgatgtc cagttcctgg tccacgtaca tatcccgccc 2221 attctgcagg tagtacaggt acagcttctc gttctgcagc tgggtgtttt ccacggggtg 2281 ttctttcagg atctggctgc ccagctcttt gatgccctct tcgatccgct tcattctctc 2341 gcggctgttc ttctgtccct tctgggtggt ctggttctct ctggccattt cgatcacgat 2401 gttctcgggc ttgtgccggc ccatcacttt cacgagctcg tccaccacct tcactgtctg 2461 caggatgccc ttcttaatgg cggggctgcc ggccagattg gcaatgtgct cgtgcaggct 2521 atcgccctgg ccggacacct gggctttctg gatgtcctct ttaaaggtca ggctgtcgtc 2581 gtggatcagc tgcatgaagt ttctgttggc gaagccgtcg gacttcagga aatccaggat 2641 tgtcttgccg gactgcttgt cccggatgcc gttgatcagc ttccggctca gcctgcccca 2701 gccggtgtat ctccgccgct tcagctgctt catcactttg tcgtcgaaca ggtgggcata 2761 ggttttcagc cgttcctcga tcatctctct gtcctcaaac agtgtcaggg tcagcacgat 2821 atcttccaga atgtcctcgt tttcctcatt gtccaggaag tccttgtcct tgataatttt 2881 cagcagatcg tggtatgtgc ccagggaggc gttgaaccga tcttccacgc cggagatttc 2941 cacggagtcg aagcactcga ttttcttgaa gtagtcctct ttcagctgct tcacggtcac 3001 tttccggttg gtcttgaaca gcaggtccac gatggctttt ttctgctcgc cgctcaggaa 3061 ggcgggcttt ctcattccct cggtcacgta tttcactttg gtcagctcgt tgtacacggt 3121 gaagtactcg tacagcaggc tgtgcttggg cagcaccttc tcgttgggca ggttcttatc 3181 gaagttggtc atccgctcga tgaagctctg ggcgctggcg cccttgtcca ccacttcctc 3241 gaagttccag ggggtgatgg tttcctcgct ctttctggtc atccaggcga atctgctgtt 3301 tcccctggcc agagggccca cgtagtaggg gatgcggaag gtcaggatct tctcgatctt 3361 ttcccggttg tccttcagga atgggtaaaa atcttcctgc cgccgcagaa tggcgtgcag 3421 ctctcccagg tggatctggt gggggatgct gccgttgtcg aaggtccgct gcttccgcag 3481 caggtcctct ctgttcagct tcacgagcag ttcctcggtg ccgtccatct tttccaggat 3541 gggcttgatg aacttgtaga actcttcctg gctggctccg ccatcgatgt agccggcgta 3601 gccgttcttg ctctggtcga agaaaatctc tttgtacttc tcaggcagct gctgccgcac 3661 gagagctttc agcagggtca ggtcctggtg gtgctcgtcg tatctcttga tcatagaggc 3721 gctcaggggg gccttggtga tctcggtgtt cactctcagg atgtcgctca gcaggatggc 3781 gtcggacagg ttcttggcgg ccagaaacag gtcggcgtac tggtcgccga tctgggccag 3841 caggttgtcc aggtcgtcgt cgtaggtgtc cttgctcagc tgcagtttgg catcctcggc 3901 caggtcgaag ttgctcttga agttgggggt caggcccagg ctcagggcaa tcaggttgcc 3961 gaacaggcca ttcttcttct cgccgggcag ctgggcgatc agattttcca gccgtctgct 4021 cttgctcagt ctggcagaca ggatggcctt ggcgtccacg ccgctggcgt tgatggggtt 4081 ttcctcgaac agctggttgt aggtctgcac cagctggatg aacagcttgt ccacgtcgct 4141 gttgtcgggg ttcaggtcgc cctcgatcag gaagtggccc cggaacttga tcatgtgggc 4201 cagggccaga tagatcagcc gcaggtcggc cttgtcggtg ctgtccacca gtttctttct 4261 caggtggtag atggtggggt acttctcgtg gtaggccacc tcgtccacga tgttgccgaa 4321 gatggggtgc cgctcgtgct tcttatcctc ttccaccagg aaggactctt ccagtctgtg 4381 gaagaagctg tcgtccacct tggccatctc gttgctgaag atctcttgca gatagcagat 4441 ccggttcttc cgtctggtgt atcttcttct ggcggttctc ttcagccggg tggcctcggc 4501 tgtttctccg ctgtcgaaca gcagggcgcc gatcaggttc ttcttgatgc tgtgccggtc 4561 ggtgttgccc agcaccttga atttcttgct gggcaccttg tactcgtcgg tgatcacggc 4621 ccagcccaca gagttggtgc cgatggccag gccgatgctg tacttcttgt ccatactagt 4681 tttcctgtgt gaaacctgct gatgtgctca gtatcttgtt atccgctcac aatgtcaatg 4741 ttatccgctc acatttataa tattttatct gattaataag atgatcttct tgagatcgtt 4801 ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt 4861 cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttggt gataagctgt 4921 caaaccagat caattcgcac tagtctgcag acgtaaaaaa agcggcgtgg ttagccgctt 4981 ttttaattgc cggagcgcaa taaaaaagcc cccggaaggt gatcttccgg gggctttctc 5041 atgcgttgct tgtattgaaa atgggggcca tgcctaccta tctgcctccc gggaagcggc 5101 atgcatttac gttgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg 5161 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc 5221 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt 5281 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa 5341 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag 5401 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact 5461 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc 5521 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga 5581 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt 5641 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg 5701 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag 5761 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat 5821 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat 5881 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc 5941 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt 6001 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa 6061 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg 6121 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct 6181 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 6241 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc 6301 tcactcatta ggcaccccac ccgggtcctt gagccccatt atcacctcca aagccttctc 6361 gtctggtcag tttcacctgt tttacgtaaa aacccgcttc ggcgggtttt tacttttggg 6421 gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac caaaggtgcg 6481 actactcttg cctactacct atcgactgag ctgaaagaat tcctaaagat ctataaatgt 6541 gagcggataa cattgacatt gtgagcggat aacaagatac ttctagtggc aggcaggttc 6601 catggcagtt taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt 6661 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ttgaattcat gtggctgacc 6721 gttctgttgt ctctcgctct tccgagtaga cgaacaataa ggcctcccta acggggggcc 6781 ttttttattg ataacaaaag tcagtgcttc cgctatttcc aaaataccgg gctaatacgg 6841 tttaaacgac ctcctggatt tgctcagaca gccttttcgt cattcgtttc agccaaaaaa 6901 cttaagaccg ccggtcttgt ccactacctt gcagtaatgc ggtggacagg atcggcggtt 6961 ttcttttctc ttctcaagaa gttcctatac tttctagaga ataggaactt cggaatagga 7021 acttcctcct gaacggccat aagaacgaag gctgtctgtt gaactctcga gccgtggaaa 7081 cggatgaagg cacgaaccca gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag 7141 tagcgtatgc gctcaaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg 7201 atcaagatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 7261 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 7321 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 7381 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 7441 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 7501 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 7561 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 7621 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 7681 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 7741 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 7801 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 7861 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 7921 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 7981 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 8041 actctggggt tcgaaatgac tcgagcatca gcttcaaaag cgctctgaag ttcctatact 8101 ttctagagaa taggaacttc ggaataggta cttcaagatc cccaattcga gctcatcgtc 8161 cgggccgcaa gctcctagcg gcggatttgt cctactcagg agagcgttca ccgacaaaca 8221 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctcaa 8281 gctagagagt cattacccca ggcgtttaag ggcaccaata actgccttaa aaaaattacg 8341 ccccgccctg ccactcatcg cagtctagct tggattctca ccaataaaaa acgcccggcg 8401 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa 8461 caggagtcca agctcagcta attaagctag cttatcgata ccgtcgacct cgaaccccac 8521 gcccctcttt aatacgacgg gcaatttgca cttcagaaaa tgaagagttt gctttagcca 8581 taacaaaagt ccagtatgct ttttcacagc ataactggac tgatttcagt ttacaactat 8641 tctgtctagt ttaagacttt attgtcatag tttagatcta ttttgttcag tttaagactt 8701 tattgtccgc ccacacccgc ttacgcaggg catccattta ttactcaacc gtaaccgatt 8761 ttgccaggtt acgcggctgg tcctctagct ggcgtaatag cgaagaggcc cgcaccgatc 8821 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc 8881 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg 8941 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 9001 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 9061 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 9121 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 9181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 9241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 9301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 9361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 9421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 9481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 9541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 9601 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 9661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 9721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 9781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 9841 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 9901 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 9961 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtcccgcg 10021 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 10081 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 10141 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 10201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 10261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 10321 gcagttattg gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 10381 atactttaga ttgatttaaa acttcatttt taatttttgc ggccgcaaga tccggccacg 10441 atgcgtccgg cgtagaggat ctgaagatca gcagttcaac ctgttgatag tacgtactaa 10501 gctctcatgt ttcacgtact aagctctcat gtttaacgta ctaagctctc atgtttaacg 10561 aactaaaccc tcatggctaa cgtactaagc tctcatggct aacgtactaa gctctcatgt 10621 ttcacgtact aagctctcat gtttgaacaa taaaattaat ataaatcagc aacttaaata 10681 gcctctaagg ttttaagttt tataagaaaa aaaagaatat ataaggcttt taaagctttt 10741 aaggtttaac ggttgtggac aacaagccag ggatgtaacg cactgagaag cccttagagc 10801 ctctcaaagc aattttgagt gacacaggaa cacttaacgg ctgacatggg aattagccat 10861 ggcatcacag tatcgtgatg acagaggcag ggagtgcggc ctttttacgg ttcctggcct 10921 tttgctggcc ttttgctcac atgttctttc ctgcgttatc aggggattcc ttaaggtata 10981 ctttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc 11041 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc 11101 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc 11161 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg 11221 ccgataagct //