LOCUS       Exported                9795 bp ds-DNA     circular SYN 19-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pTn7C187
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Jason Peters
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 19, 2018 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    1..48
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(182..832)
                     /codon_start=1
                     /transl_table=11
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="cat (CmR)"
                     /note="Gram positive inducible"
                     /db_xref="GI:20804332"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="CAD10262.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     CDS             1139..5242
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /note="dCas9"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5245..5344
                     /codon_start=1
                     /note="3xMyc"
                     /translation="AAEQKLISEEDLEQKLISEEDLEQKLISEEDL"
     terminator      5376..5430
                     /note="L3S2P24 terminator"
     misc_feature    5461..6498
                     /note="ICE downstream homology"
     oriT            6511..6619
                     /note="incP origin of transfer"
     rep_origin      6888..7276
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(7577..8437)
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="Bla"
                     /note="bla"
                     /db_xref="GI:403391486"
                     /protein_id="AFR43358.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8438..8542)
                     /gene="bla"
                     /note="AmpR promoter"
     misc_feature    8574..9430
                     /note="ICE upstream homology"
     terminator      9462..9556
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      9659..9745
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaagtaccta ttccgaagtt cctattctct agaaagtata ggaacttcag agcgcttttg
       61 aagctgatgc tcgaggcgta gaggatctgg agctgtaata taaaaacctt cttcaactaa
      121 cggggcaggt tagtgacatt agaaaaccga ctgtaaaaag tacagtcggc attatctcat
      181 attataaaag ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc
      241 ctgcatgata accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg
      301 aattaccttt attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat
      361 tattgatatt taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag
      421 cattttcagg tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat
      481 cagaaaggta taaatcataa aactctttga agtcattctt tacaggagtc caaataccag
      541 agaatgtttt agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac
      601 ctaactctcc gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg
      661 tcactaagaa aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata
      721 aaacactaat atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat
      781 taaatatctc ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg
      841 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag
      901 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc
      961 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaagacc
     1021 acattaaaaa atgtggtctt ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct
     1081 tttctttctt atcttgataa taagggtaac tattgccgat gataagctgt caactagtat
     1141 ggataagaaa tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat
     1201 cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca
     1261 cagtatcaaa aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc
     1321 gactcgtctc aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta
     1381 tctacaggag attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact
     1441 tgaagagtct tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa
     1501 tatagtagat gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa
     1561 attggtagat tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat
     1621 gattaagttt cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt
     1681 ggacaaacta tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat
     1741 taacgcaagt ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg
     1801 attagaaaat ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct
     1861 cattgctttg tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga
     1921 tgctaaatta cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca
     1981 aattggagat caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt
     2041 actttcagat atcctaagag taaatactga aataactaag gctcccctat cagcttcaat
     2101 gattaaacgc tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca
     2161 acaacttcca gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg
     2221 ttatattgat gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga
     2281 aaaaatggat ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa
     2341 gcaacggacc tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc
     2401 tattttgaga agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga
     2461 aaaaatcttg acttttcgaa tcccttatta tgttggtcca ttggcgcgtg gcaatagtcg
     2521 ttttgcatgg atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt
     2581 tgtcgataaa ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa
     2641 tcttccaaat gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta
     2701 taacgaattg acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc
     2761 aggtgaacag aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt
     2821 taagcaatta aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc
     2881 aggagttgaa gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat
     2941 taaagataaa gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt
     3001 aacattgacc ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca
     3061 cctctttgat gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg
     3121 tttgtctcga aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga
     3181 ttttttgaaa tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag
     3241 tttgacattt aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca
     3301 tgaacatatt gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt
     3361 aaaagttgtt gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat
     3421 tgaaatggca cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat
     3481 gaaacgaatc gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt
     3541 tgaaaatact caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga
     3601 catgtatgtg gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatgccat
     3661 tgttccacaa agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga
     3721 taaaaatcgt ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa
     3781 ctattggaga caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac
     3841 gaaagctgaa cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt
     3901 ggttgaaact cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac
     3961 taaatacgat gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa
     4021 attagtttct gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta
     4081 ccatcatgcc catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata
     4141 tccaaaactt gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat
     4201 gattgctaag tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa
     4261 tatcatgaac ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc
     4321 tctaatcgaa actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc
     4381 cacagtgcgc aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtaca
     4441 gacaggcgga ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc
     4501 tcgtaaaaaa gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta
     4561 ttcagtccta gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa
     4621 agagttacta gggatcacaa ttatggaaag aagttccttt gaaaaaaatc cgattgactt
     4681 tttagaagct aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata
     4741 tagtcttttt gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca
     4801 aaaaggaaat gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca
     4861 ttatgaaaag ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca
     4921 gcataagcat tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat
     4981 tttagcagat gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc
     5041 aatacgtgaa caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc
     5101 cgctgctttt aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga
     5161 agttttagat gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga
     5221 tttgagtcag ctaggaggtg acgcggccgc ggagcagaaa ctcatctctg aagaagatct
     5281 ggaacaaaag ttgatttcag aagaagatct ggaacagaag ctcatctctg aggaagatct
     5341 gtaataaggc gcgcctcctt aatgggactt gcagcctcgg taccaaattc cagaaaagac
     5401 acccgaaagg gtgttttttc gttttggtcc cacagaatga gcatcatggc tctagtcgac
     5461 cgggtagggg catagcttag ataattggaa aagaggaaaa aagcttaatc ttttttcgaa
     5521 ggttaagctt tttcttttat ttataaaaag tgaactaact atcagaaaga aattatatta
     5581 aattttattt ttttgtttaa aaagtagatt atataaaggc aagctaggtg ggggaaaata
     5641 tgtttaaaaa agaaaaagtc acagaataca tttggactat actaatacca acaatcatca
     5701 cttttatcat tagttgggtt gggtcttatt acaatggtac ttcgacagtt agtattggac
     5761 aacctacaaa agtttccggt cagtatatca cgccaataaa tataagtccc tatcatgata
     5821 ttaaggaatt aagaataact tttccgcaaa aactagatgt aaaacaaatt agttcaaatg
     5881 agcctataaa tgtaaaatca gataagaaca atataggagt tgaaagtaat tccacttttg
     5941 agattgcgaa aatcgttgaa aataatagcg ttcagttgct aattacaaca caaaaaaagt
     6001 taaacgataa ggaaattaga attgataaaa atggaaataa catttctgta aattatgaat
     6061 ctcagattgt taatcctgca aaaaaacaat taatcaatct tataattacg tcatctattt
     6121 attttataat gcttaatata ctagcattga ttatgaacaa aagatgggat aagtattatg
     6181 caaaaatgaa aaatgaaatc aaagaatttg aggataatgc aaaagatctt gataaaaaat
     6241 caaagaagaa aagcgaggaa ttatcggagc tgcgaaagac cttgaaccaa gcgtttgagg
     6301 aaactgatag gataaaatat catgagaaga aaaaacaaat cctcctctta gctaagttaa
     6361 acgattataa aaaagaacta accttttgga gaaatacaat aagaaaagtt ctttatgaac
     6421 ttcctgatgg agataaaaaa gcagataaac taatagggac agttacatca tctttaaaaa
     6481 cgtacggtac agtcgaaaag taaagcttat cggccagcct cgcagagcag gattcccgtt
     6541 gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc
     6601 tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac
     6661 cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat
     6721 aatgaccccg aagcagggtt atgcagcgga aagtatacct taaggaatcc cctgataacg
     6781 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcac
     6841 tccctgcctc tgtcatcacg atactgtgat gccatggcta attcccatgt cagccgttaa
     6901 gtgttcctgt gtcactcaaa attgctttga gaggctctaa gggcttctca gtgcgttaca
     6961 tccctggctt gttgtccaca accgttaaac cttaaaagct ttaaaagcct tatatattct
     7021 tttttttctt ataaaactta aaaccttaga ggctatttaa gttgctgatt tatattaatt
     7081 ttattgttca aacatgagag cttagtacgt gaaacatgag agcttagtac gttagccatg
     7141 agagcttagt acgttagcca tgagggttta gttcgttaaa catgagagct tagtacgtta
     7201 aacatgagag cttagtacgt gaaacatgag agcttagtac gtactatcaa caggttgaac
     7261 tgctgatctt cagatcctct acgccggacg catcgtggcc ggatcttgcg gccgcaaaaa
     7321 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7381 ccaatgctta atcagtgagg caccaataac tgcctttgat cttttctacg gggtctgacg
     7441 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7501 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7561 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7621 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7681 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7741 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7801 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7861 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7921 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7981 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8041 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8101 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8161 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8221 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8281 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8341 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8401 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8461 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8521 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcttctaac
     8581 tacttatttt aaagcagtct ggattgtttg ggtaattcat aaaaaaataa aagaaagaag
     8641 gaggaataga gtttttcttt tttttgtttg caatgttact gtcaagtcgc aaaagaattg
     8701 ctatgaccga aaaaaaaccg caaaggaata gatataaggt acctttttgc aattcatctt
     8761 tgtaaaataa aggttattct gacataatac aattaatgta aaaattcgca caattttatg
     8821 taaggatggg ggaattttct tgcggggtgt tttcttagat aaagataaaa ttccgtacga
     8881 cttagtcacg aaaaagttaa atgaatggta tacatcaata aaaaatgatc aagttgagca
     8941 agccgagatt ataaaaacag aagtagagaa agaattgtta aacatggaag aaaatcaaga
     9001 tgccctgtta tattatcaac tattagaatt tagacatgag ataatgctga gttatatgaa
     9061 atctaaggaa atagaagatc tcaataatgc ttatgagact ataaaagaaa ttgagaagca
     9121 agggcaatta actggcatgt tggaatacta tttttacttt tttaagggta tgtacgagtt
     9181 taggcgtaaa gaattaattt cagcgataag tgcttatcga atagctgaat caaagttgtc
     9241 agaagttgag gatgaaatag agaaagcaga gttttttttc aaagtgtcct atgtatatta
     9301 ttatatgaaa caaacatact tctccatgaa ttatgcaaat cgtgcactca aaatatttag
     9361 agagtatgaa gaatatgctg tccagactgt gcgttgtcaa tttattgtag caggaaactt
     9421 gatcgagtga atcgataagc tagcttaatt agctgagctt ggactcctgt tgatagatcc
     9481 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
     9541 ttttattggt gagaatccaa gctagactgc gatgagtggc agggcggggc gtaatttttt
     9601 taaggcagtt attggtgccc ttaaacgcct ggggtaatga ctctctagct tgaggcatca
     9661 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     9721 gaacgctctc ctgagtagga caaatccgcc gctaggagct tgcggcccgg acgatgagct
     9781 cgaattgggg atctt
//
