LOCUS PHAGE-P_CMVt_N-HA_hE6API_AA1-64 8010 bp DNA circular UNA 23-OCT-2018
DEFINITION an expression clone created from PHAGE-P_CMVt_N-HA_GAW and
pDONR223_hE6API_AA1-64.
ACCESSION urn.local...24-9nut9w4
VERSION urn.local...24-9nut9w4
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
LTR 1..636
/ApEinfo_fwdcolor="#bfbf00"
/ApEinfo_revcolor="#bfbf00"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="HIV LTR"
Gene 454..634
/Imported_from="Pla
sMapper"
/Transferred_From="HIV-1_5_LTR, truncHIV-1_3_LTR"
/Transferred_Similarity="100.00%"
/label="HIV-1_5_LTR; truncHIV-1_3_LTR"
misc_feature 637..1155
/ApEinfo_fwdcolor="#962dff"
/ApEinfo_revcolor="#962dff"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="Psi packaging signal"
Gene 745..789
/Imported_from="Pla
sMapper"
/Transferred_From="HIV-1_psi_pack"
/Transferred_Similarity="100.00%"
/label="HIV-1_psi_pack"
misc_feature 1303..1536
/Imported_from="Pla
sMapper"
/Transferred_From="RRE reg"
/Transferred_Similarity="100.00%"
/label="RRE reg"
/note="Geneious type: Regulatory Sequence"
misc_feature 1303..1536
/ApEinfo_fwdcolor="#962dff"
/ApEinfo_revcolor="#962dff"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="RRE"
misc_feature 2034..2211
/ApEinfo_fwdcolor="#962dff"
/ApEinfo_revcolor="#962dff"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="HIV cpPu (Trip)"
promoter 2218..2874
/ApEinfo_fwdcolor="#ff0000"
/ApEinfo_revcolor="#ff0000"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="CMVt promoter"
misc_feature 2736..2775
/Imported_from="Pla
sMapper"
/Transferred_From="tetO reg"
/Transferred_Similarity="100.00%"
/label="tetO reg"
/note="Geneious type: Regulatory Sequence"
protein_bind 2736..2754
/ApEinfo_fwdcolor="#808080"
/ApEinfo_revcolor="#808080"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="Tet operator"
protein_bind 2757..2775
/ApEinfo_label="Tet operator"
/ApEinfo_fwdcolor="#808080"
/ApEinfo_revcolor="#808080"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="Tet operator(1)"
Tag complement(2908..2934)
/Imported_from="Pla
sMapper"
/Transferred_From="HA tag"
/Transferred_Similarity="100.00%"
/label="HA tag"
misc_structure 2908..2934
/ApEinfo_fwdcolor="#00c400"
/ApEinfo_revcolor="#00c400"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="HA tag"
attB1 2951..2971
/annotation_group="attB"
/label="attB1"
CDS 2977..3168
/created_by="gustavo"
/modified_by="gustavo"
/label="hE6-AP I (AA 1-64)"
attB2 complement(3174..3194)
/annotation_group="attB"
/label="attB2"
promoter 3212..3551
/ApEinfo_fwdcolor="#ff0000"
/ApEinfo_revcolor="#ff0000"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="mPGK promoter"
CDS 3734..4330
/ApEinfo_fwdcolor="#0000ff"
/ApEinfo_revcolor="#0000ff"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="Puro(R)"
misc_feature 4399..4990
/ApEinfo_fwdcolor="#962dff"
/ApEinfo_revcolor="#962dff"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="WPRE"
LTR 5062..5297
/ApEinfo_fwdcolor="#bfbf00"
/ApEinfo_revcolor="#bfbf00"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="dU3 LTR"
Gene 5063..5115
/Imported_from="Pla
sMapper"
/Transferred_From="delta_U3"
/Transferred_Similarity="100.00%"
/label="delta_U3"
Gene 5116..5296
/Imported_from="Pla
sMapper"
/Transferred_From="HIV-1_5_LTR, truncHIV-1_3_LTR"
/Transferred_Similarity="100.00%"
/label="HIV-1_5_LTR; truncHIV-1_3_LTR"
Promoter 5536..5564
/Imported_from="Pla
sMapper"
/Transferred_From="amp prom"
/Transferred_Similarity="100.00%"
/label="amp prom"
promoter 5572..5606
/ApEinfo_fwdcolor="#ff0000"
/ApEinfo_revcolor="#ff0000"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="P-Bla promoter prokaryotic"
CDS 5606..6463
/ApEinfo_fwdcolor="#0000ff"
/ApEinfo_revcolor="#0000ff"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="Amp(R)"
rep_origin 6561..7243
/ApEinfo_fwdcolor="#808080"
/ApEinfo_revcolor="#808080"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="ColE1 origin"
Promoter 7672..7701
/Imported_from="Pla
sMapper"
/Transferred_From="lac prom"
/Transferred_Similarity="100.00%"
/label="lac prom"
misc_binding 7706..7728
/ApEinfo_fwdcolor="#0000ff"
/ApEinfo_revcolor="#0000ff"
/ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
/label="LacO"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggaaccaaaa
2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
2881 agcgtttaaa cgctagccac catggcgtac ccatacgatg ttccagatta cgctgctcga
2941 ggttatcaaa caagtttgta caaaaaagtt ggcaccatga agcgagcagc tgcaaagcat
3001 ctaatagaac gctactacca ccagttaact gagggctgtg gaaatgaagc ctgcacgaat
3061 gagttttgtg cttcctgtcc aacttttctt cgtatggata ataatgcagc agctattaaa
3121 gccctcgagc tttataagat taatgcaaaa ctctgtgatc ctcatccctg acccaacttt
3181 cttgtacaaa gtggttcgat aacgaattct accgggtagg ggaggcgctt ttcccaaggc
3241 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
3301 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
3361 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
3421 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
3481 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
3541 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
3601 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
3661 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
3721 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
3781 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
3841 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
3901 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
3961 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
4021 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
4081 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
4141 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
4201 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
4261 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
4321 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
4381 ccatgcatcg atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg
4441 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
4501 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
4561 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
4621 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
4681 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
4741 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
4801 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
4861 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
4921 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
4981 ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
5041 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
5101 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
5161 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
5221 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
5281 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
5341 aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc
5401 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
5461 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
5521 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
5581 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
5641 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
5701 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
5761 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
5821 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
5881 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
5941 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
6001 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
6061 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
6121 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
6181 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
6241 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
6301 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
6361 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
6421 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
6481 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
6541 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
6601 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
6661 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
6721 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
6781 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
6841 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
6901 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
6961 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
7021 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
7081 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
7141 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
7201 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
7261 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
7321 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
7381 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
7441 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag
7501 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
7561 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
7621 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
7681 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
7741 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
7801 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
7861 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
7921 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
7981 ttgttaaata tgtactacaa acttagtagt
//