LOCUS       PHAGE-P_CMVt_N-HA_hE6API_AA55-376        8781 bp    DNA     circular UNA 23-OCT-2018
DEFINITION  an expression clone created from PHAGE-P_CMVt_N-HA_GAW and
            pDONR223_hE6API_AA55-376.
ACCESSION   urn.local...31-9nutvft
VERSION     urn.local...31-9nutvft
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             1..636
                     /ApEinfo_fwdcolor="#bfbf00"
                     /ApEinfo_revcolor="#bfbf00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV LTR"
     Gene            454..634
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_5_LTR, truncHIV-1_3_LTR"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
     misc_feature    637..1155
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Psi packaging signal"
     Gene            745..789
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_psi_pack"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_psi_pack"
     misc_feature    1303..1536
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="RRE reg"
                     /Transferred_Similarity="100.00%"
                     /label="RRE reg"
                     /note="Geneious type: Regulatory Sequence"
     misc_feature    1303..1536
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="RRE"
     misc_feature    2034..2211
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV cpPu (Trip)"
     promoter        2218..2874
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="CMVt promoter"
     misc_feature    2736..2775
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="tetO reg"
                     /Transferred_Similarity="100.00%"
                     /label="tetO reg"
                     /note="Geneious type: Regulatory Sequence"
     protein_bind    2736..2754
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Tet operator"
     protein_bind    2757..2775
                     /ApEinfo_label="Tet operator"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Tet operator(1)"
     Tag             complement(2908..2934)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HA tag"
                     /Transferred_Similarity="100.00%"
                     /label="HA tag"
     misc_structure  2908..2934
                     /ApEinfo_fwdcolor="#00c400"
                     /ApEinfo_revcolor="#00c400"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HA tag"
     attB1           2951..2971
                     /annotation_group="attB"
                     /label="attB1"
     CDS             2974..3939
                     /created_by="gustavo"
                     /modified_by="gustavo"
                     /label="hE6-AP I (AA 55-376)"
     attB2           complement(3945..3965)
                     /annotation_group="attB"
                     /label="attB2"
     promoter        3983..4322
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="mPGK promoter"
     CDS             4505..5101
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Puro(R)"
     misc_feature    5170..5761
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="WPRE"
     LTR             5833..6068
                     /ApEinfo_fwdcolor="#bfbf00"
                     /ApEinfo_revcolor="#bfbf00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="dU3 LTR"
     Gene            5834..5886
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="delta_U3"
                     /Transferred_Similarity="100.00%"
                     /label="delta_U3"
     Gene            5887..6067
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_5_LTR, truncHIV-1_3_LTR"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
     Promoter        6307..6335
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     promoter        6343..6377
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="P-Bla promoter prokaryotic"
     CDS             6377..7234
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Amp(R)"
     rep_origin      7332..8014
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ColE1 origin"
     Promoter        8443..8472
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lac prom"
                     /Transferred_Similarity="100.00%"
                     /label="lac prom"
     misc_binding    8477..8499
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="LacO"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggaaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     2881 agcgtttaaa cgctagccac catggcgtac ccatacgatg ttccagatta cgctgctcga
     2941 ggttatcaaa caagtttgta caaaaaagtt ggcattaatg caaaactctg tgatcctcat
     3001 ccctccaaga aaggagcaag ctcagcttac cttgagaact cgaaaggtgc ccccaacaac
     3061 tcctgctctg agataaaaat gaacaagaaa ggcgctagaa ttgattttaa agatgtgact
     3121 tacttaacag aagagaaggt atatgaaatt cttgaattat gtagagaaag agaggattat
     3181 tcccctttaa tccgtgttat tggaagagtt ttttctagtg ctgaggcatt ggtacagagc
     3241 ttccggaaag ttaaacaaca caccaaggaa gaactgaaat ctcttcaagc aaaagatgaa
     3301 gacaaagatg aagatgaaaa ggaaaaagct gcatgttctg ctgctgctat ggaagaagac
     3361 tcagaagcat cttcctcaag gataggtgat agctcacagg gagacaacaa tttgcaaaaa
     3421 ttaggccctg atgatgtgtc tgtggatatt gatgccatta gaagggtcta caccagattg
     3481 ctctctaatg aaaaaattga aactgccttt ctcaatgcac ttgtatattt gtcacctaac
     3541 gtggaatgtg acttgacgta tcacaatgta tactctcgag atcctaatta tctgaatttg
     3601 ttcattatcg taatggagaa tagaaatctc cacagtcctg aatatctgga aatggctttg
     3661 ccattatttt gcaaagcgat gagcaagcta ccccttgcag cccaaggaaa actgatcaga
     3721 ctgtggtcta aatacaatgc agaccagatt cggagaatga tggagacatt tcagcaactt
     3781 attacttata aagtcataag caatgaattt aacagtcgaa atctagtgaa tgatgatgat
     3841 gccattgttg ctgcttcgaa gtgcttgaaa atggtttact atgcaaatgt agtgggaggg
     3901 gaagtggaca caaatcacaa tgaagaagat gatgaagagt gacccaactt tcttgtacaa
     3961 agtggttcga taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     4021 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     4081 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     4141 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     4201 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     4261 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     4321 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     4381 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     4441 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     4501 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     4561 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     4621 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     4681 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     4741 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     4801 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     4861 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     4921 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     4981 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     5041 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     5101 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     5161 gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5221 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5281 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5341 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5401 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5461 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5521 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     5581 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5641 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5701 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     5761 tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5821 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     5881 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5941 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6001 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6061 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6121 atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg
     6181 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     6241 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6301 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6361 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6421 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6481 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6541 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6601 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6661 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6721 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6781 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6841 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6901 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6961 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7021 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7081 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7141 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7201 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7261 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7321 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7381 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7441 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7501 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7561 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7621 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7681 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7741 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7801 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7861 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7921 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7981 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8041 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8101 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8161 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8221 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8281 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8341 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8521 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8581 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     8641 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     8701 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     8761 atgtactaca aacttagtag t
//
