LOCUS       PHAGE-P_CMVt_N-HA_hE6API_AA481-852        8931 bp    DNA     circular UNA 23-OCT-2018
DEFINITION  an expression clone created from PHAGE-P_CMVt_N-HA_GAW and
            pDONR223_hE6API_AA481-852.
ACCESSION   urn.local...4k-9nuunyf
VERSION     urn.local...4k-9nuunyf
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             1..636
                     /ApEinfo_fwdcolor="#bfbf00"
                     /ApEinfo_revcolor="#bfbf00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV LTR"
     Gene            454..634
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_5_LTR, truncHIV-1_3_LTR"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
     misc_feature    637..1155
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Psi packaging signal"
     Gene            745..789
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_psi_pack"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_psi_pack"
     misc_feature    1303..1536
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="RRE reg"
                     /Transferred_Similarity="100.00%"
                     /label="RRE reg"
                     /note="Geneious type: Regulatory Sequence"
     misc_feature    1303..1536
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="RRE"
     misc_feature    2034..2211
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV cpPu (Trip)"
     promoter        2218..2874
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="CMVt promoter"
     misc_feature    2736..2775
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="tetO reg"
                     /Transferred_Similarity="100.00%"
                     /label="tetO reg"
                     /note="Geneious type: Regulatory Sequence"
     protein_bind    2736..2754
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Tet operator"
     protein_bind    2757..2775
                     /ApEinfo_label="Tet operator"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Tet operator(1)"
     Tag             complement(2908..2934)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HA tag"
                     /Transferred_Similarity="100.00%"
                     /label="HA tag"
     misc_structure  2908..2934
                     /ApEinfo_fwdcolor="#00c400"
                     /ApEinfo_revcolor="#00c400"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HA tag"
     attB1           2951..2971
                     /annotation_group="attB"
                     /label="attB1"
     CDS             2974..4089
                     /created_by="gustavo"
                     /modified_by="gustavo"
                     /label="hE6-AP I (AA 481-852)"
     attB2           complement(4095..4115)
                     /annotation_group="attB"
                     /label="attB2"
     promoter        4133..4472
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="mPGK promoter"
     CDS             4655..5251
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Puro(R)"
     misc_feature    5320..5911
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="WPRE"
     LTR             5983..6218
                     /ApEinfo_fwdcolor="#bfbf00"
                     /ApEinfo_revcolor="#bfbf00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="dU3 LTR"
     Gene            5984..6036
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="delta_U3"
                     /Transferred_Similarity="100.00%"
                     /label="delta_U3"
     Gene            6037..6217
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_5_LTR, truncHIV-1_3_LTR"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
     Promoter        6457..6485
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     promoter        6493..6527
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="P-Bla promoter prokaryotic"
     CDS             6527..7384
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Amp(R)"
     rep_origin      7482..8164
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ColE1 origin"
     Promoter        8593..8622
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lac prom"
                     /Transferred_Similarity="100.00%"
                     /label="lac prom"
     misc_binding    8627..8649
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="LacO"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggaaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     2881 agcgtttaaa cgctagccac catggcgtac ccatacgatg ttccagatta cgctgctcga
     2941 ggttatcaaa caagtttgta caaaaaagtt ggcgaacgaa gaatcactgt tctctacagc
     3001 ttagttcaag gacagcagtt gaatccatat ttgagactca aagttagacg tgaccatatc
     3061 atagatgatg cacttgtccg gctagagatg atcgctatgg aaaatcctgc agacttgaag
     3121 aagcagttgt atgtggaatt tgaaggagaa caaggagttg atgagggagg tgtttccaaa
     3181 gaattttttc agctggttgt ggaggaaatc ttcaatccag atattggtat gttcacatac
     3241 gatgaatcta caaaattgtt ttggtttaat ccatcttctt ttgaaactga gggtcagttt
     3301 actctgattg gcatagtact gggtctggct atttacaata actgtatact ggatgtacat
     3361 tttcccatgg ttgtctacag gaagctaatg gggaaaaaag gaacttttcg tgacttggga
     3421 gactctcacc cagttctata tcagagttta aaagatttat tggagtatga agggaatgtg
     3481 gaagatgaca tgatgatcac tttccagata tcacagacag atctttttgg taacccaatg
     3541 atgtatgatc taaaggaaaa tggtgataaa attccaatta caaatgaaaa caggaaggaa
     3601 tttgtcaatc tttattctga ctacattctc aataaatcag tagaaaaaca gttcaaggct
     3661 tttcggagag gttttcatat ggtgaccaat gaatctccct taaagtactt attcagacca
     3721 gaagaaattg aattgcttat atgtggaagc cggaatctag atttccaagc actagaagaa
     3781 actacagaat atgacggtgg ctataccagg gactctgttc tgattaggga gttctgggaa
     3841 atcgttcatt catttacaga tgaacagaaa agactcttct tgcagtttac aacgggcaca
     3901 gacagagcac ctgtgggagg actaggaaaa ttaaagatga ttatagccaa aaatggccca
     3961 gacacagaaa ggttacctac atctcatact tgctttaatg tgcttttact tccggaatac
     4021 tcaagcaaag aaaaacttaa agagagattg ttgaaggcca tcacgtatgc caaaggattt
     4081 ggcatgctgt aacccaactt tcttgtacaa agtggttcga taacgaattc taccgggtag
     4141 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     4201 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     4261 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     4321 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     4381 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     4441 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     4501 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     4561 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     4621 ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg
     4681 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4741 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4801 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4861 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4921 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4981 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     5041 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     5101 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     5161 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     5221 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     5281 gaccgaaagg agcgcacgac cccatgcatc gatagatcct aatcaacctc tggattacaa
     5341 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5401 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5461 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5521 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5581 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5641 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5701 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     5761 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5821 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     5881 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
     5941 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6001 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6181 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6241 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
     6301 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     6361 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6421 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6481 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6541 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6601 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6661 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6721 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     6781 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6841 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6901 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6961 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7021 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7081 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7141 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7201 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7261 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7321 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7381 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7441 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7501 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7561 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7621 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7681 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7741 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7801 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7861 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7921 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7981 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8041 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8101 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8161 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8221 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8281 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8341 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
     8401 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8461 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
     8521 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8581 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8641 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
     8701 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8761 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     8821 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     8881 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
