LOCUS       PHAGE-P_CMVt_N-HA_hE6APII_AA1-87        8079 bp    DNA     circular UNA 23-OCT-2018
DEFINITION  an expression clone created from PHAGE-P_CMVt_N-HA_GAW and
            pDONR223_hE6APII_AA1-87.
ACCESSION   urn.local...5q-9nuv1ag
VERSION     urn.local...5q-9nuv1ag
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             1..636
                     /ApEinfo_fwdcolor="#bfbf00"
                     /ApEinfo_revcolor="#bfbf00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV LTR"
     Gene            454..634
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_5_LTR, truncHIV-1_3_LTR"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
     misc_feature    637..1155
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Psi packaging signal"
     Gene            745..789
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_psi_pack"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_psi_pack"
     misc_feature    1303..1536
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="RRE reg"
                     /Transferred_Similarity="100.00%"
                     /label="RRE reg"
                     /note="Geneious type: Regulatory Sequence"
     misc_feature    1303..1536
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="RRE"
     misc_feature    2034..2211
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV cpPu (Trip)"
     promoter        2218..2874
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="CMVt promoter"
     misc_feature    2736..2775
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="tetO reg"
                     /Transferred_Similarity="100.00%"
                     /label="tetO reg"
                     /note="Geneious type: Regulatory Sequence"
     protein_bind    2736..2754
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Tet operator"
     protein_bind    2757..2775
                     /ApEinfo_label="Tet operator"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Tet operator(1)"
     Tag             complement(2908..2934)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HA tag"
                     /Transferred_Similarity="100.00%"
                     /label="HA tag"
     misc_structure  2908..2934
                     /ApEinfo_fwdcolor="#00c400"
                     /ApEinfo_revcolor="#00c400"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HA tag"
     attB1           2951..2971
                     /annotation_group="attB"
                     /label="attB1"
     CDS             2977..3237
                     /created_by="gustavo"
                     /modified_by="gustavo"
                     /label="hE6-AP II (AA 1-87)"
     attB2           complement(3243..3263)
                     /annotation_group="attB"
                     /label="attB2"
     promoter        3281..3620
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="mPGK promoter"
     CDS             3803..4399
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Puro(R)"
     misc_feature    4468..5059
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="WPRE"
     LTR             5131..5366
                     /ApEinfo_fwdcolor="#bfbf00"
                     /ApEinfo_revcolor="#bfbf00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="dU3 LTR"
     Gene            5132..5184
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="delta_U3"
                     /Transferred_Similarity="100.00%"
                     /label="delta_U3"
     Gene            5185..5365
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_5_LTR, truncHIV-1_3_LTR"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
     Promoter        5605..5633
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     promoter        5641..5675
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="P-Bla promoter prokaryotic"
     CDS             5675..6532
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Amp(R)"
     rep_origin      6630..7312
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ColE1 origin"
     Promoter        7741..7770
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lac prom"
                     /Transferred_Similarity="100.00%"
                     /label="lac prom"
     misc_binding    7775..7797
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="LacO"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggaaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     2881 agcgtttaaa cgctagccac catggcgtac ccatacgatg ttccagatta cgctgctcga
     2941 ggttatcaaa caagtttgta caaaaaagtt ggcaccatgg agaagctgca ccagtgttat
     3001 tggaaatcag gagaacctca gtctgacgac attgaagcta gccgaatgaa gcgagcagct
     3061 gcaaagcatc taatagaacg ctactaccac cagttaactg agggctgtgg aaatgaagcc
     3121 tgcacgaatg agttttgtgc ttcctgtcca acttttcttc gtatggataa taatgcagca
     3181 gctattaaag ccctcgagct ttataagatt aatgcaaaac tctgtgatcc tcatccctga
     3241 cccaactttc ttgtacaaag tggttcgata acgaattcta ccgggtaggg gaggcgcttt
     3301 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     3361 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     3421 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     3481 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     3541 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     3601 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     3661 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     3721 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     3781 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     3841 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     3901 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     3961 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4021 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4081 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4141 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4201 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     4261 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4321 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4381 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     4441 cgcacgaccc catgcatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag
     4501 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4561 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4621 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4681 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4741 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4801 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4861 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4921 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4981 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5041 ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat
     5101 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5161 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5221 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5281 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5341 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     5401 taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct
     5461 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5521 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5581 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5641 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5701 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5761 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5821 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5881 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     5941 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6001 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6061 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6121 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6181 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6241 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6301 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6361 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6421 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6481 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6541 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6601 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6841 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7381 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7441 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7501 ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt
     7561 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7621 tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa
     7681 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7741 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7801 cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca
     7861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     7921 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     7981 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     8041 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
