LOCUS       PHAGE-P_CMVt_N-HA_hE6APIII_AA1-84        8070 bp    DNA     circular UNA 23-OCT-2018
DEFINITION  an expression clone created from PHAGE-P_CMVt_N-HA_GAW and
            pDONR223_hE6APIII_AA1-84.
ACCESSION   urn.local...6y-9nuvatk
VERSION     urn.local...6y-9nuvatk
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             1..636
                     /ApEinfo_fwdcolor="#bfbf00"
                     /ApEinfo_revcolor="#bfbf00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV LTR"
     Gene            454..634
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_5_LTR, truncHIV-1_3_LTR"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
     misc_feature    637..1155
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Psi packaging signal"
     Gene            745..789
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_psi_pack"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_psi_pack"
     misc_feature    1303..1536
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="RRE reg"
                     /Transferred_Similarity="100.00%"
                     /label="RRE reg"
                     /note="Geneious type: Regulatory Sequence"
     misc_feature    1303..1536
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="RRE"
     misc_feature    2034..2211
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV cpPu (Trip)"
     promoter        2218..2874
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="CMVt promoter"
     misc_feature    2736..2775
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="tetO reg"
                     /Transferred_Similarity="100.00%"
                     /label="tetO reg"
                     /note="Geneious type: Regulatory Sequence"
     protein_bind    2736..2754
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Tet operator"
     protein_bind    2757..2775
                     /ApEinfo_label="Tet operator"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Tet operator(1)"
     Tag             complement(2908..2934)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HA tag"
                     /Transferred_Similarity="100.00%"
                     /label="HA tag"
     misc_structure  2908..2934
                     /ApEinfo_fwdcolor="#00c400"
                     /ApEinfo_revcolor="#00c400"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HA tag"
     attB1           2951..2971
                     /annotation_group="attB"
                     /label="attB1"
     CDS             2977..3228
                     /created_by="gustavo"
                     /modified_by="gustavo"
                     /label="hE6-AP III (AA 1-84)"
     attB2           complement(3234..3254)
                     /annotation_group="attB"
                     /label="attB2"
     promoter        3272..3611
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="mPGK promoter"
     CDS             3794..4390
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Puro(R)"
     misc_feature    4459..5050
                     /ApEinfo_fwdcolor="#962dff"
                     /ApEinfo_revcolor="#962dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="WPRE"
     LTR             5122..5357
                     /ApEinfo_fwdcolor="#bfbf00"
                     /ApEinfo_revcolor="#bfbf00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="dU3 LTR"
     Gene            5123..5175
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="delta_U3"
                     /Transferred_Similarity="100.00%"
                     /label="delta_U3"
     Gene            5176..5356
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="HIV-1_5_LTR, truncHIV-1_3_LTR"
                     /Transferred_Similarity="100.00%"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
     Promoter        5596..5624
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     promoter        5632..5666
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="P-Bla promoter prokaryotic"
     CDS             5666..6523
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Amp(R)"
     rep_origin      6621..7303
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ColE1 origin"
     Promoter        7732..7761
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lac prom"
                     /Transferred_Similarity="100.00%"
                     /label="lac prom"
     misc_binding    7766..7788
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="LacO"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggaaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     2881 agcgtttaaa cgctagccac catggcgtac ccatacgatg ttccagatta cgctgctcga
     2941 ggttatcaaa caagtttgta caaaaaagtt ggcaccatgg ccacagcttg taaaagatca
     3001 ggagaacctc agtctgacga cattgaagct agccgaatga agcgagcagc tgcaaagcat
     3061 ctaatagaac gctactacca ccagttaact gagggctgtg gaaatgaagc ctgcacgaat
     3121 gagttttgtg cttcctgtcc aacttttctt cgtatggata ataatgcagc agctattaaa
     3181 gccctcgagc tttataagat taatgcaaaa ctctgtgatc ctcatccctg acccaacttt
     3241 cttgtacaaa gtggttcgat aacgaattct accgggtagg ggaggcgctt ttcccaaggc
     3301 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     3361 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     3421 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     3481 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     3541 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     3601 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     3661 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     3721 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     3781 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3841 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3901 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3961 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     4021 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4081 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4141 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4201 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4261 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4321 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4381 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     4441 ccatgcatcg atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4501 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4561 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4621 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4681 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4741 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4801 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4861 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4921 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4981 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5041 ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5101 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     5161 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5221 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5281 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5341 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5401 aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc
     5461 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5521 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5581 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5641 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5701 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5761 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5821 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5881 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     5941 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6001 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6061 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6121 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6181 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6241 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6301 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6361 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6421 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6481 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6541 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6601 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6661 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6721 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6781 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6841 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6901 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6961 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7021 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7081 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7141 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7201 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7261 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7321 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7381 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7441 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     7501 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag
     7561 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7621 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
     7681 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7741 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7801 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
     7861 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
     7921 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
     7981 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     8041 ttgttaaata tgtactacaa acttagtagt
//
