LOCUS       Exported                2278 bp ds-DNA     circular SYN 17-OCT-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   SynthSys Center
  TITLE     Direct Submission
  JOURNAL   Exported Oct 17, 2018 from SnapGene 4.2.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             132..1142
                     /codon_start=1
                     /label=SpecR
                     /label=Sm/Sp no DraIII
                     /label=Sm/Sp\no\DraIII
                     /note="vntifkey=4"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    1302..1916
                     /label=ori (pMB1)
                     /label=rep (pMB1)
                     /label=rep\(pMB1)
                     /note="vntifkey=21"
     misc_feature    2149..2152
                     /label=AATG
     misc_feature    2153..2179
                     /label=UP FLANK LINKER
                     /label=UP L
     misc_feature    2180..2183
                     /label=CGCT
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      181 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      241 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      301 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag
      361 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      421 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      481 cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      541 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      601 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      661 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      721 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
      781 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
      841 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
      901 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
      961 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1021 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1081 atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat
     1141 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1201 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1261 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1321 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1381 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1441 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1501 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1561 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1621 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1681 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1741 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1801 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1861 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1921 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1981 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2041 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     2101 cgcctctccc cgcgcgttgg ccgattcatt aatcactctg tggtctctaa tgtcggtcac
     2161 atgtgcatcc tcgatctcac gctagagacc acgaagtggc tcttcagtgg acgaaagggc
     2221 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgt
//

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