LOCUS       Exported                2333 bp ds-DNA     circular SYN 14-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   ravi
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 14, 2019 from SnapGene 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2237..2326
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             220..1230
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SpecR
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1401..1989
                     /label=ori (pMB1)
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2237..2240
                     /label=GTTT
     misc_feature    2238..2322
                     /label=srRNA HFQ handle
                     /label=HFQ binding site
     misc_feature    2323..2326
                     /label=CGCT
ORIGIN
        1 acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat
       61 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      121 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      181 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc
      241 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
      301 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
      361 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
      421 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
      481 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
      541 ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg
      601 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
      661 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
      721 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
      781 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
      841 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
      901 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
      961 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     1021 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     1081 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     1141 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa
     1201 ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt
     1261 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1321 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1381 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1441 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1501 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1561 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1621 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1681 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1741 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1801 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1861 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1921 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1981 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2041 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2101 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2161 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2221 tcactctgtg gtctctgttt ctgttgggcc attgcattgc cactgatttt ccaacatata
     2281 aaaagacaag cccgaacagt cgtccgggct ttttttctcg agcgctagag acc
//
