LOCUS       Exported                3251 bp ds-DNA     circular SYN 19-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2019 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    227..1237
                     /label=SpecR
     rep_origin      1408..1996
                     /label=ori (pMB1)
                     /label=ori (pBM1)
                     /label=ori
     misc_feature    2244..2247
                     /label=GCTT
     misc_feature    2248..3247
                     /label=NS2 DOWN FLANK
                     /label=DOWN FLANK
     misc_feature    2867
                     /label=Possible A
     misc_feature    3248..3251
                     /label=CGCT
ORIGIN
        1 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
       61 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
      241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
      301 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
      361 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
      421 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
      481 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      541 tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt
      601 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      661 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      721 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      781 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      841 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      901 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      961 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1021 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1081 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     1141 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta
     1201 cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata
     1261 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1321 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1381 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1441 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1501 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1561 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1621 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1681 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1741 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1801 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1861 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1921 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1981 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2041 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2101 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2161 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     2221 tcattaatca ctctgtggtc tcagcttatc ccaaagaccc tccactaagc gatcgtgtag
     2281 aggccactga acagacaagc acaaaaatac aatcctccca gactatggtt tgtgtccgtt
     2341 gatcgcactc ggcatgaaaa taagtccatt ttgtcgattg accctaccat aaaaactcaa
     2401 gcttgatctt ttatggatcc tctttctttc gatctggctt tgtaaacata agtttatgtt
     2461 aattagttaa agatgccctg aattttatca gtttcatgaa gcttctttta acctcagatt
     2521 aattaggcac taattttata gaaaaaagct tcattatata gaatttctaa aaatcaagct
     2581 ttaaagggtt gaccttcctt ttgaaaggta ataaaactga agtacaaaca aatacgtaag
     2641 taacgaaaag gagtaagccg caatctgcct cagtagaaac acgtattttt accccaaaag
     2701 aagtattcta ttcatctttc tcctttgtga gaaggttcat ggagatcctt ctggggcact
     2761 tgaaacctct acttgcaagg atcaaagcga catatgtacg tgtttttcca aaaaaatccc
     2821 agaagtaggg agtaattcct ctactgagat ttcaagaaaa ttaagtgttc tgaggcttgg
     2881 tttatacggg aaatttagac ttgatggttt gggtcttacc ttttcctagc agcaagacta
     2941 aattaattag ttacgcattt gttgaaatca aactgaattc tattgaggat tccttacaat
     3001 ggctaaattc aattcttcta caatcacatt cgcaaccatc atcgcttttg ctgccatcgg
     3061 tcaagccgca caggctggcg aaagctacgt ccgcaacaca ttcgaacaca gcacttccaa
     3121 aacgacttct aaccttgatc tggaaagcaa agattggtat cacggctctc gtgaatggtc
     3181 taactatgct tacaaagaat actacgatgg aactgtaaca accagtgtag atggtgaact
     3241 ctctagtcgc t
//

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