LOCUS       pFGRNA-AsCpf1-Sp        3161 bp    DNA     circular     26-OCT-2018
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12526187|
COMMENT     VNTDBDATE|-12526187|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pFGRNA-AsCpf1-SpCas9|
COMMENT     VNTAUTHORNAME|Jiyeon Kweon|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pFGRNA-AsCpf1-SpCas9" 0 3161 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList "BsaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3161 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 350 1 1026 0 0 "")) 
COMMENT       (CObList 
COMMENT        #1=(CFSignal (CObList) "Amp" 21 0 0 2168 3025 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1497..2354") 
COMMENT        #2=(CFSignal (CObList) "pUCori" 21 0 0 1347 2020 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "676..1349") 
COMMENT        #3=(CFSignal (CObList) "pU6" 21 0 0 93 325 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "93..325")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #4=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT            (CObjectList 
COMMENT             #5=(CRefLinePar 
COMMENT                 (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                  "pFGRNA-AsCpf1-SpCas9" 2) 5 "" 0 4) 
COMMENT             #6=(CFolderPar 
COMMENT                 (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                  (CObjectList 
COMMENT                   #7=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                       "DNA 'pFGRNA-AsCpf1-SpCas9'" 1) 
COMMENT                   #8=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Local object" 
COMMENT                       1) 
COMMENT                   #9=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                       "Created: 10/26/18 04:02\277\300\310\304" 1) 
COMMENT                   #10=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "Last Modified: 10/26/18 04:02\277\300\310\304" 1) 
COMMENT                   #11=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "length: 3161 bp" 1) 
COMMENT                   #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "storage type: Basic" 1) 
COMMENT                   #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "form: Circular" 1))) "General Description") 
COMMENT             #14=(CFolderPar 
COMMENT                  (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                   (CObjectList 
COMMENT                    #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Original Source Database: GenBank" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Modification Date in the Original DB: 17-NOV-2011" 1))) 
COMMENT                  "Standard Fields") 
COMMENT             #17=(CRefLinePar 
COMMENT                  (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                  0 0) 
COMMENT             #18=(CFolderPar 
COMMENT                  (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                  "Annotations") 
COMMENT             #19=(CFolderPar 
COMMENT                  (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                   (CObjectList 
COMMENT                    #20=(CFolderPar 
COMMENT                         (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                          (CObjectList 
COMMENT                           #21=(CFolderPar 
COMMENT                                (CGroupPar (CParagraph 93 (3 #3# 0) 1 2 2 0 194) 
COMMENT                                 (CObjectList 
COMMENT                                  #22=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 93    End: 325 " 1) 
COMMENT                                  #23=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #24=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   93..325" 1))) "pU6") 
COMMENT                           #25=(CFolderPar 
COMMENT                                (CGroupPar 
COMMENT                                 (CParagraph 1347 (3 #2# 0) 1 2 2 0 194) 
COMMENT                                 (CObjectList 
COMMENT                                  #26=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 1347  End: 2020" 1) 
COMMENT                                  #27=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #28=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   676..1349" 1))) "pUCori") 
COMMENT                           #29=(CFolderPar 
COMMENT                                (CGroupPar 
COMMENT                                 (CParagraph 2168 (3 #1# 0) 1 2 2 0 194) 
COMMENT                                 (CObjectList 
COMMENT                                  #30=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 2168  End: 3025" 1) 
COMMENT                                  #31=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   1497..2354" 1))) "Amp"))) 
COMMENT                         "Misc. Feature (3 total)"))) "Feature Map") 
COMMENT             #33=(CFolderPar 
COMMENT                  (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                   (CObjectList 
COMMENT                    #34=(CRSFolderPar 
COMMENT                         (CFolderPar 
COMMENT                          (CGroupPar (CParagraph 59053480 (8 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #35=(CGroupPar (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                 (CObjectList 
COMMENT                                  #36=(CLinePar 
COMMENT                                       (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                       " N1: 350 (on complementary strand)" 1) 
COMMENT                                  #37=(CLinePar 
COMMENT                                       (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                       " N2: 1026 " 1))))) "BsaI: 2 sites") 7 11 
COMMENT                         "GGTCTCNNNNNN" "CCAGAGNNNNNN"))) 
COMMENT                  "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #38=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT               "@N (@S)" 0) 
COMMENT          #39=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT               "@N" 0) 
COMMENT          #40=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT               "@L bp" 0) 
COMMENT          #41=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT               "@N" 0) 
COMMENT          #42=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT               (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #43=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT               (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #44=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT               (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #45=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT               (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #46=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT               (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #47=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT               (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #48=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 5 
COMMENT               "@N (@S)" 0) 
COMMENT          #49=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 0 16777215 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 1 
COMMENT               2 "?" 0) 
COMMENT          #50=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 0) 
COMMENT          #51=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 1 
COMMENT               65535 "@N (@H)" 0) 
COMMENT          #52=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT               "@F bp (molecule @L bp)" 0) 
COMMENT          #53=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT               "Fragment of @N" 0) 
COMMENT          #54=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #55=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT               0 0) 
COMMENT          #56=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT               0) 
COMMENT          #57=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT               (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #58=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #59=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #60=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #61=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #62=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #63=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #64=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #65=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 1 
COMMENT               3 "@T @N " 0) 
COMMENT          #66=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #67=(CWidgetStyle "Annotation" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 0 
COMMENT               0) 
COMMENT          #68=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT               "@N" 0) 
COMMENT          #69=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777215 0) 
COMMENT               0 0 0) 
COMMENT          #70=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT               "@N" 0) 
COMMENT          #71=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 
COMMENT               0 1 65535 "@N" 0) 
COMMENT          #72=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT               "@N" 0) 
COMMENT          #73=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT               (LOGBRUSH 0 16777113 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT               (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #74=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT               1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #75=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 1 
COMMENT               1 "@N" 0) 
COMMENT          #76=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 5 
COMMENT               "@N (@S)" 0) 
COMMENT          #77=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT               "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 
COMMENT         -2490) 1 1 0 1 1 
COMMENT        (mapper: 14.8464 -12.7283 40.0378 40.0378 0.01 10 -1 3161 3161 1 0 0) 
COMMENT        #78=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1866932325 100) 
COMMENT             (CObjectList 
COMMENT              #79=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -501 100) 
COMMENT                   (CObjectList 
COMMENT                    #80=(CAxis 
COMMENT                         (CWideLine (CWidget 0 (0 0) 1 2 0 0 #44# 58667716 0) 
COMMENT                          (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                          6.27617 6.27417 1 0.0214037) 0.198456) 
COMMENT                    #81=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #41# 0 100) 
COMMENT                         (LOGPEN 0 0 0) 1 
COMMENT                         (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                         1.74233 0.833333 0 "pFGRNA-AsCpf1-SpCas9" "@N" 1 0 
COMMENT                         0.871165 0 -10 13.1875 1.34873 Nil) 
COMMENT                    #82=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #40# 255 100) 
COMMENT                         (LOGPEN 0 0 0) 1 
COMMENT                         (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                         1.74233 0.555556 0 "3161 bp" "@L bp" 16 0 -0.871165 0 
COMMENT                         -10.8 3.24693 0.949103 Nil)) (CObjectList)) 
COMMENT              #83=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil 1919885412 100) 
COMMENT                   (CObjectList 
COMMENT                    #84=(CGroupWidget (CWidget 21 (7 21 0) 1 2 0 0 Nil -737 100) 
COMMENT                         (CObjectList 
COMMENT                          #85=(CWideArrow 
COMMENT                               (CWideLine 
COMMENT                                (CWidget 0 (3 #1# 0) 1 2 0 0 #43# 0 100) 
COMMENT                                (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334 0) 1 
COMMENT                                0.2699 1.97265 1 0.082322) 0.8 1.8 0) 
COMMENT                          #86=(CLabel (CWidget 0 (0 0) 1 2 0 0 #39# 0 100) 
COMMENT                               (LOGPEN 0 0 0) 1 
COMMENT                               (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                               1.74233 0.666667 0 "Amp" "@N" 1 -12.0395 5.22699 
COMMENT                               0 -10 2.12299 1.07399 #85#) 
COMMENT                          #87=(CWideArrow 
COMMENT                               (CWideLine 
COMMENT                                (CWidget 0 (3 #2# 0) 1 2 0 0 #43# 0 100) 
COMMENT                                (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334 0) 1 
COMMENT                                2.26438 3.60197 1 0.082322) 0.8 1.8 0) 
COMMENT                          #88=(CLabel (CWidget 0 (0 0) 1 2 0 0 #39# 6 100) 
COMMENT                               (LOGPEN 0 0 0) 1 
COMMENT                               (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                               1.74233 0.666667 0 "pUCori" "@N" 1 -4.83809 
COMMENT                               -12.1963 0 -10 3.34684 1.07399 #87#) 
COMMENT                          #89=(CWideArrow 
COMMENT                               (CWideLine 
COMMENT                                (CWidget 0 (3 #3# 0) 1 2 0 0 #43# 0 100) 
COMMENT                                (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334 0) 1 
COMMENT                                5.6282 6.09061 1 0.082322) 0.8 1.8 0) 
COMMENT                          #90=(CLabel (CWidget 0 (0 0) 1 2 0 0 #39# 0 100) 
COMMENT                               (LOGPEN 0 0 0) 1 
COMMENT                               (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                               1.74233 0.666667 0 "pU6" "@N" 1 7.40565 10.454 0 
COMMENT                               -10 1.92318 1.07399 #89#)) (CObjectList))) 
COMMENT                   (CObjectList)) 
COMMENT              #91=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -321 100) 
COMMENT                   (CObjectList 
COMMENT                    #92=(CGroupWidget 
COMMENT                         (CWidget 2 (10 #0# 0) 1 2 0 0 Nil -1018 100) 
COMMENT                         (CObjectList 
COMMENT                          #93=(CScratch (CWidget 1 (1 #0# 1) 1 2 0 0 #46# 0 100) 
COMMENT                               (LOGPEN 0 6 10053171) 8 1 5.58057 1.9 0.082322 1) 
COMMENT                          #94=(CLabel 
COMMENT                               (CWidget 0 (0 0) 1 2 0 0 #38# 1718633331 100) 
COMMENT                               (LOGPEN 0 0 13408563) 1 
COMMENT                               (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                               1.74233 0.555556 0 "{\\i Bsa}I (350)" "@N (@S)" 5 
COMMENT                               10.6444 8.71165 0 -10 4.22101 0.949103 #93#) 
COMMENT                          #95=(CScratch (CWidget 2 (1 #0# 2) 1 2 0 0 #46# 0 100) 
COMMENT                               (LOGPEN 0 6 10053171) 8 1 4.23901 1.9 0.082322 1) 
COMMENT                          #96=(CLabel (CWidget 0 (0 0) 1 2 0 0 #38# 399 100) 
COMMENT                               (LOGPEN 0 0 13408563) 1 
COMMENT                               (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                               1.74233 0.555556 0 "{\\i Bsa}I (1026)" "@N (@S)" 
COMMENT                               5 13.1347 -5.22699 0 -10 4.69556 0.949103 #95#)) 
COMMENT                         (CObjectList))) (CObjectList)) 
COMMENT              #97=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -112 100) 
COMMENT                   (CObjectList) (CObjectList)) 
COMMENT              #98=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1866932325 100) 
COMMENT                   (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 
COMMENT       (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    2168..3025
                     /vntifkey="21"
                     /label=Amp
     misc_feature    1347..2020
                     /vntifkey="21"
                     /label=pUCori
     misc_feature    93..325
                     /vntifkey="21"
                     /label=pU6
BASE COUNT      812 a       760 c       780 g       809 t 
ORIGIN
        1 gacgaagact caattgtcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc 
       61 ctcgagcggc cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata 
      121 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat 
      181 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat 
      241 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg 
      301 ctttatatat cttgtggaaa ggacgaaaca ccgtaatttc tactcttgta gatggagacc 
      361 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 
      421 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 
      481 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 
      541 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 
      601 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 
      661 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 
      721 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 
      781 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 
      841 acggccagtg ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag 
      901 ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 
      961 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atagatccgg 
     1021 tctccgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa 
     1081 aaagtggcac cgagtcggtg cttttttcta gattcgcgat gtacgggcca gatatacgcg 
     1141 ttgacattga ttattgacta gttgtcttcc tgcattaatg aatcggccaa cgcgcgggga 
     1201 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     1261 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     1321 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     1381 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     1441 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     1501 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     1561 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc 
     1621 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     1681 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     1741 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     1801 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
     1861 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     1921 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     1981 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     2041 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     2101 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     2161 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     2221 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     2281 gccccagtgc tgcaatgata ccgcgagatc cacgctcacc ggctccagat ttatcagcaa 
     2341 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     2401 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     2461 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     2521 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     2581 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     2641 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     2701 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     2761 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     2821 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     2881 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     2941 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     3001 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     3061 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     3121 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c 
//
