LOCUS       Exported                7436 bp ds-DNA     circular SYN 26-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 26, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    188..193
                     /label=SpeI
     misc_feature    819..1876
                     /label=psi plus pack2
     misc_feature    956..961
                     /label=SpeI
     misc_feature    1295..1789
                     /label=gag
     misc_feature    1881..2106
                     /label=psi plus pack 3prime
     misc_feature    1900..1905
                     /label=AgeI
     misc_feature    2019..2038
                     /label=pBMN Fwd Primer
     misc_feature    2108..2115
                     /label=Kozak
     misc_feature    2111..2116
                     /label=NcoI
     CDS             2116..2820
                     /label=mCherry
     misc_feature    2116..2817
                     /label=mCherry
     misc_feature    2546..2551
                     /label=NcoI
     misc_feature    2754..2775
                     /label=mCh Fwd Internal Primer
     misc_feature    2834..2839
                     /label=XhoI
     misc_feature    2843..2848
                     /label=HindIII
     misc_feature    2848..4575
                     /label=OPTN D474N
     CDS             3162..3173
                     /label=FactorXa site
     misc_feature    4027..4032
                     /label=HindIII
     misc_feature    4579..4584
                     /label=BamHI
     misc_feature    complement(4648..4666)
                     /label=pBMN Rev Primer
     rep_origin      complement(5527..6155)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6310..6969)
                     /label=AmpR
     misc_feature    6796..6814
                     /label=Isotherm Amp2
     misc_feature    complement(6796..6814)
                     /label=Isotherm Amp1
ORIGIN
        1 caatgtgcat gaatgctgca atattcctgt taccaaagct agtataaata aaaatagata
       61 aacgtggaaa ttacttagag tttctgtcat taacgtttcc ttcctcagtt gacaacataa
      121 atgcgctgct gagaagccag tttgcatctg tcaggatcaa tttcccatta tgccagtcat
      181 attaattact agtcaattag ttgattttta tttttgacat atacatgtga aagaccccac
      241 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
      301 actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
      361 aaagcggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
      421 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      481 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
      541 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
      601 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
      661 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
      721 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
      781 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
      841 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
      901 ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag
      961 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc
     1021 ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga
     1081 gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg agggatatgt
     1141 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
     1201 ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
     1261 ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga ctgttaccac
     1321 tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc
     1381 ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa
     1441 cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa
     1501 ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga
     1561 agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc
     1621 tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg
     1681 atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc catatgagat
     1741 cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac
     1801 taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg
     1861 gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta
     1921 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg
     1981 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc
     2041 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag
     2101 actgccgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     2161 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     2221 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2281 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     2341 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     2401 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     2461 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     2521 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     2581 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     2641 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     2701 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     2761 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     2821 tccggactca gatctcgagc tcaagcttcc catcaacctc tcagctgcct cactgaaaag
     2881 gaggacagcc ccagtgaaag cacaggaaat ggaccccccc acctggccca cccaaacctg
     2941 gacacgttta ccccggagga gctgctgcag cagatgaaag agctcctgac cgagaaccac
     3001 cagctgaaag aagccatgaa gctaaataat caagccatga aagggagatt tgaggagctt
     3061 tcggcctgga cagagaaaca gaaggaagaa cgccagtttt ttgagataca gagcaaagaa
     3121 gcaaaagagc gtctaatggc cttgagtcat gagaatgaga aattgaagga agagcttgga
     3181 aaactaaaag ggaaatcaga aaggtcatct gaggacccca ctgatgactc caggcttccc
     3241 agggccgaag cggagcagga aaaggaccag ctcaggaccc aggtggtgag gctacaagca
     3301 gagaaggcag acctgttggg catcgtgtct gaactgcagc tcaagctgaa ctccagcggc
     3361 tcctcagaag attcctttgt tgaaattagg atggctgaag gagaagcaga agggtcagta
     3421 aaagaaatca agcatagtcc tgggcccacg agaacagtct ccactggcac ggcattgtct
     3481 aaatatagga gcagatctgc agatggggcc aagaattact tcgaacatga ggagttaact
     3541 gtgagccagc tcctgctgtg cctaagggaa gggaatcaga aggtggagag acttgaagtt
     3601 gcactcaagg aggccaaaga aagagtttca gattttgaaa agaaaacaag taatcgttct
     3661 gagattgaaa cccagacaga ggggagcaca gagaaagaga atgatgaaga gaaaggcccg
     3721 gagactgttg gaagcgaagt ggaagcactg aacctccagg tgacatctct gtttaaggag
     3781 cttcaagagg ctcatacaaa actcagcgaa gctgagctaa tgaagaagag acttcaagaa
     3841 aagtgtcagg cccttgaaag gaaaaattct gcaattccat cagagttgaa tgaaaagcaa
     3901 gagcttgttt atactaacaa aaagttagag ctacaagtgg aaagcatgct atcagaaatc
     3961 aaaatggaac aggctaaaac agaggatgaa aagtccaaat taactgtgct acagatgaca
     4021 cacaacaagc ttcttcaaga acataataat gcattgaaaa caattgagga actaacaaga
     4081 aaagagtcag aaaaagtgga cagggcagtg ctgaaggaac tgagtgaaaa actggaactg
     4141 gcagagaagg ctctggcttc caaacagctg caaatggatg aaatgaagca aaccattgcc
     4201 aagcaggaag aggacctgga aaccatgacc atcctcaggg ctcagatgga agtttactgt
     4261 tctaattttc atgctgaaag agcagcgaga gagaaaattc atgaggaaaa ggagcaactg
     4321 gcattgcagc tggcagttct gctgaaagag aatgatgctt tcgaagacgg aggcaggcag
     4381 tccttgatgg agatgcagag tcgtcatggg gcgagaacaa gtgactctga ccagcaggct
     4441 taccttgttc aaagaggagc tgaggacagg gactggcggc aacagcggaa tattccgatt
     4501 cattcctgcc ccaagtgtgg agaggttctg cctgacatag acacgttaca gattcacgtg
     4561 atggattgca tcatttaagg atccaccgga tctagataac tgatcacgat aaaataaaag
     4621 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     4681 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     4741 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     4801 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
     4861 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     4921 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     4981 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     5041 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
     5101 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     5161 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     5221 cccgtcagcg ggggtctttc attcacatgc agcatgtatc aaaattaatt tggttttttt
     5281 tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga
     5341 gaggcggttt gcgtattggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5521 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5641 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     6001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6241 tttaaattaa aaatgaagtt tgcgcaaatc aatctaaagt atatatgagt aaacttggtc
     6301 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6361 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6421 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6481 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6541 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6601 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6661 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6721 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6781 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6841 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6901 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     6961 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7021 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7081 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7141 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7201 tcagggttat tgtctcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     7261 tcttcaagaa cagctttgct cttaggagtt tcctaataca tcccaaactc aaatatataa
     7321 agcatttgac ttgttctatg ccctaggggg cggggggaag ctaagccagc tttttttaac
     7381 atttaaaatg ttaattccat tttaaatgca cagatgtttt tatttcataa gggttt
//
