LOCUS       Exported                7439 bp ds-DNA     circular SYN 26-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 26, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..198
                     /label=SpeI
     misc_feature    824..1881
                     /label=psi plus pack2
     misc_feature    961..966
                     /label=SpeI
     misc_feature    1300..1794
                     /label=gag
     misc_feature    1886..2111
                     /label=psi plus pack 3prime
     misc_feature    1905..1910
                     /label=AgeI
     misc_feature    2024..2043
                     /label=pBMN Fwd Primer
     misc_feature    2113..2120
                     /label=Kozak
     misc_feature    2116..2121
                     /label=NcoI
     CDS             2121..2825
                     /label=mCherry
     misc_feature    2121..2822
                     /label=mCherry
     misc_feature    2551..2556
                     /label=NcoI
     misc_feature    2759..2780
                     /label=mCh Fwd Internal Primer
     misc_feature    2839..2844
                     /label=XhoI
     misc_feature    2848..2853
                     /label=HindIII
     misc_feature    2856..3702
                     /label=front OPTN F178A
     CDS             3170..3181
                     /label=FactorXa site
     misc_feature    3703..4583
                     /label=back OPTN D474N
     misc_feature    4035..4040
                     /label=HindIII
     misc_feature    4587..4592
                     /label=BamHI
     misc_feature    complement(4656..4674)
                     /label=pBMN Rev Primer
     rep_origin      complement(5535..6163)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6318..6977)
                     /label=AmpR
     misc_feature    6804..6822
                     /label=Isotherm Amp2
     misc_feature    complement(6804..6822)
                     /label=Isotherm Amp1
ORIGIN
        1 ggtttcaatg tgcatgaatg ctgcaatatt cctgttacca aagctagtat aaataaaaat
       61 agataaacgt ggaaattact tagagtttct gtcattaacg tttccttcct cagttgacaa
      121 cataaatgcg ctgctgagaa gccagtttgc atctgtcagg atcaatttcc cattatgcca
      181 gtcatattaa ttactagtca attagttgat ttttattttt gacatataca tgtgaaagac
      241 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
      301 acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac agctgaatat
      361 gggccaaagc ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      421 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
      481 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
      541 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
      601 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
      661 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
      721 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
      781 tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg
      841 atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa
      901 cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt
      961 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa
     1021 cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga
     1081 cctgagtcca aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga
     1141 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg
     1201 ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt
     1261 tgtctctgtc tgactgtgtt tctgtatttg tctgaaaata tgggcccggg ccagactgtt
     1321 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac
     1381 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc
     1441 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag
     1501 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc
     1561 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct
     1621 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg
     1681 cctcgatcct ccctttatcc agccctcact ccttctctag gcgcccccat atggccatat
     1741 gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga catgacaaga
     1801 gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt ccagcacgaa
     1861 gtctggagac ctctggcggc agcctaccaa gaacaactgg accgaccggt ggtacctcac
     1921 ccttaccgag tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct
     1981 cgctggaaag gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc
     2041 atcgcagctt ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc
     2101 tctagactgc cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     2161 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     2221 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     2281 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     2341 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     2401 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     2461 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     2521 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     2581 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     2641 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     2701 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     2761 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     2821 acaagtccgg actcagatct cgagctcaag cttggtccca tcaacctctc agctgcctca
     2881 ctgaaaagga ggacagcccc agtgaaagca caggaaatgg acccccccac ctggcccacc
     2941 caaacctgga cacgtttacc ccggaggagc tgctgcagca gatgaaagag ctcctgaccg
     3001 agaaccacca gctgaaagaa gccatgaagc taaataatca agccatgaaa gggagatttg
     3061 aggagctttc ggcctggaca gagaaacaga aggaagaacg ccagtttttt gagatacaga
     3121 gcaaagaagc aaaagagcgt ctaatggcct tgagtcatga gaatgagaaa ttgaaggaag
     3181 agcttggaaa actaaaaggg aaatcagaaa ggtcatctga ggaccccact gatgactcca
     3241 ggcttcccag ggccgaagcg gagcaggaaa aggaccagct caggacccag gtggtgaggc
     3301 tacaagcaga gaaggcagac ctgttgggca tcgtgtctga actgcagctc aagctgaact
     3361 ccagcggctc ctcagaagat tccgctgttg aaattaggat ggctgaagga gaagcagaag
     3421 ggtcagtaaa agaaatcaag catagtcctg ggcccacgag aacagtctcc actggcacgg
     3481 cattgtctaa atataggagc agatctgcag atggggccaa gaattacttc gaacatgagg
     3541 agttaactgt gagccagctc ctgctgtgcc taagggaagg gaatcagaag gtggagagac
     3601 ttgaagttgc actcaaggag gccaaagaaa gagtttcaga ttttgaaaag aaaacaagta
     3661 atcgttctga gattgaaacc cagacagagg ggagcacaga gaaagagaat gatgaagaga
     3721 aaggcccgga gactgttgga agcgaagtgg aagcactgaa cctccaggtg acatctctgt
     3781 ttaaggagct tcaagaggct catacaaaac tcagcgaagc tgagctaatg aagaagagac
     3841 ttcaagaaaa gtgtcaggcc cttgaaagga aaaattctgc aattccatca gagttgaatg
     3901 aaaagcaaga gcttgtttat actaacaaaa agttagagct acaagtggaa agcatgctat
     3961 cagaaatcaa aatggaacag gctaaaacag aggatgaaaa gtccaaatta actgtgctac
     4021 agatgacaca caacaagctt cttcaagaac ataataatgc attgaaaaca attgaggaac
     4081 taacaagaaa agagtcagaa aaagtggaca gggcagtgct gaaggaactg agtgaaaaac
     4141 tggaactggc agagaaggct ctggcttcca aacagctgca aatggatgaa atgaagcaaa
     4201 ccattgccaa gcaggaagag gacctggaaa ccatgaccat cctcagggct cagatggaag
     4261 tttactgttc taattttcat gctgaaagag cagcgagaga gaaaattcat gaggaaaagg
     4321 agcaactggc attgcagctg gcagttctgc tgaaagagaa tgatgctttc gaagacggag
     4381 gcaggcagtc cttgatggag atgcagagtc gtcatggggc gagaacaagt gactctgacc
     4441 agcaggctta ccttgttcaa agaggagctg aggacaggga ctggcggcaa cagcggaata
     4501 ttccgattca ttcctgcccc aagtgtggag aggttctgcc tgacatagac acgttacaga
     4561 ttcacgtgat ggattgcatc atttaaggat ccaccggatc tagataactg atcacgataa
     4621 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     4681 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     4741 tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
     4801 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
     4861 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     4921 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
     4981 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     5041 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     5101 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     5161 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     5221 attgactacc cgtcagcggg ggtctttcat tcacatgcag catgtatcaa aattaatttg
     5281 gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg
     5341 cgcggggaga ggcggtttgc gtattggcgc tcttccgctt cctcgctcac tgactcgctg
     5401 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5461 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5521 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5581 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5641 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5701 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5761 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5821 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5881 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5941 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     6001 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6061 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6121 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6181 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6241 tagatccttt taaattaaaa atgaagtttg cgcaaatcaa tctaaagtat atatgagtaa
     6301 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6361 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6421 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6481 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6541 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6601 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6661 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6721 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6781 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6841 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6901 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     6961 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7021 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7081 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7141 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7201 agcatttatc agggttattg tctcatgaca ttaacctata aaaataggcg tatcacgagg
     7261 ccctttcgtc ttcaagaaca gctttgctct taggagtttc ctaatacatc ccaaactcaa
     7321 atatataaag catttgactt gttctatgcc ctagggggcg gggggaagct aagccagctt
     7381 tttttaacat ttaaaatgtt aattccattt taaatgcaca gatgttttta tttcataag
//
