LOCUS       Exported                7046 bp ds-DNA     circular SYN 26-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 26, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    188..193
                     /label=SpeI
     misc_feature    819..1876
                     /label=psi plus pack2
     misc_feature    956..961
                     /label=SpeI
     misc_feature    1295..1789
                     /label=gag
     misc_feature    1881..2106
                     /label=psi plus pack 3prime
     misc_feature    1900..1905
                     /label=AgeI
     misc_feature    2019..2038
                     /label=pBMN Fwd Primer
     misc_feature    2108..2115
                     /label=Kozak
     misc_feature    2111..2116
                     /label=NcoI
     CDS             2116..2820
                     /label=mCherry
     misc_feature    2116..2817
                     /label=mCherry
     misc_feature    2546..2551
                     /label=NcoI
     misc_feature    2754..2775
                     /label=mCh Fwd Internal Primer
     misc_feature    2834..2839
                     /label=XhoI
     misc_feature    2843..2848
                     /label=HindIII
     misc_feature    2851..4185
                     /label=NDP52 C443K
     misc_feature    2851..3231
                     /label=NDP52 SKICH
     misc_feature    3128..3133
                     /label=HindIII
     misc_feature    3241..3261
                     /label=NDP52 LIR V136
     misc_feature    3472..3858
                     /label=NDP52 Leucine Zipper
     misc_feature    3583..3588
                     /label=HindIII
     misc_feature    3691..3696
                     /label=XhoI
     misc_feature    4189..4194
                     /label=BamHI
     misc_feature    complement(4258..4276)
                     /label=pBMN Rev Primer
     rep_origin      complement(5137..5765)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(5920..6579)
                     /label=AmpR
     misc_feature    6406..6424
                     /label=Isotherm Amp2
     misc_feature    complement(6406..6424)
                     /label=Isotherm Amp1
ORIGIN
        1 caatgtgcat gaatgctgca atattcctgt taccaaagct agtataaata aaaatagata
       61 aacgtggaaa ttacttagag tttctgtcat taacgtttcc ttcctcagtt gacaacataa
      121 atgcgctgct gagaagccag tttgcatctg tcaggatcaa tttcccatta tgccagtcat
      181 attaattact agtcaattag ttgattttta tttttgacat atacatgtga aagaccccac
      241 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
      301 actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
      361 aaagcggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
      421 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      481 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
      541 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
      601 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
      661 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
      721 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
      781 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
      841 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
      901 ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag
      961 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc
     1021 ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga
     1081 gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg agggatatgt
     1141 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
     1201 ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
     1261 ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga ctgttaccac
     1321 tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc
     1381 ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa
     1441 cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa
     1501 ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga
     1561 agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc
     1621 tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg
     1681 atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc catatgagat
     1741 cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac
     1801 taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg
     1861 gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta
     1921 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg
     1981 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc
     2041 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag
     2101 actgccgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     2161 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     2221 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2281 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     2341 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     2401 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     2461 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     2521 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     2581 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     2641 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     2701 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     2761 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     2821 tccggactca gatctcgagc tcaagcttgg gaggagacca tcaaagatcc ccccacatca
     2881 gctgtcttgc tggatcactg tcatttctct caggtcatct ttaacagtgt ggagaagttc
     2941 tacatccctg gaggggacgt cacatgtcat tataccttca cccagcattt catccctcgt
     3001 cgaaaggatt ggattggcat ctttagagtg gggtggaaga caacccgtga gtattacacc
     3061 ttcatgtggg ttactttgcc cattgaccta aacaacaaat cagctaaaca gcaggaagtc
     3121 caattcaaag cttactacct gcccaaggat gatgagtatt accagttctg ctatgtggat
     3181 gaggatggtg tggtccgggg agcaagtatt cctttccaat tccgtccaga aaatgaggaa
     3241 gacatcctgg ttgttaccac tcagggagag gtggaagaga ttgagcagca caacaaggag
     3301 ctttgcaaag aaaaccagga gctgaaggac agctgtatca gcctccagaa gcagaactca
     3361 gacatgcagg ctgagctcca aaagaagcag gaggagctag aaaccctaca gagcatcaat
     3421 aagaagttgg aactgaaagt gaaagaacag aaggactatt gggagacaga gctgcttcaa
     3481 ctgaaagaac aaaaccagaa gatgtcctca gaaaatgaga agatgggaat cagagtggat
     3541 cagcttcagg cccagctgtc aactcaagag aaagaaatgg agaagcttgt tcagggagat
     3601 caagataaga cagagcagtt agagcagctg aaaaaggaaa atgaccacct ctttctcagt
     3661 ttagctgaac agaggaagga ccagaagaag ctcgagcaga cagtggagca aatgaagcag
     3721 aatgaaacta ctgcaatgaa gaaacaacag gaattaatgg atgaaaactt tgacctgtca
     3781 aaaagactga gtgagaacga aattatatgt aatgctctgc agagacagaa agagagattg
     3841 gaaggagaaa atgatctttt gaagagggag aacagcagat tgctcagtta catgggtctg
     3901 gattttaatt ctttgccgta tcaagtacct acttcagatg aaggaggcgc aagacaaaat
     3961 ccaggacttg cctatggaaa cccatattct ggtatccaag aaagttcttc ccccagcccg
     4021 ctctccatca agaaatgccc tatctgcaaa gcagatgata tttgtgatca caccttggag
     4081 caacagcaga tgcagcccct ttgtttcaat tgtccaattt gtgacaagat cttcccagct
     4141 acagagaagc agatctttga agaccacgtg ttcaaacact ctctctgagg atccaccgga
     4201 tctagataac tgatcacgat aaaataaaag attttattta gtctccagaa aaagggggga
     4261 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     4321 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
     4381 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     4441 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     4501 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     4561 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     4621 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     4681 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     4741 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     4801 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc attcacatgc
     4861 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
     4921 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc gctcttccgc
     4981 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5041 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5101 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5161 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5221 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5281 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5341 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5401 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5461 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5521 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5581 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5641 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5701 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5761 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5821 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt tgcgcaaatc
     5881 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5941 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6001 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6061 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6121 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6181 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6241 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6301 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6361 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6421 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6481 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     6541 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6601 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6661 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6721 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6781 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga cattaaccta
     6841 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa cagctttgct cttaggagtt
     6901 tcctaataca tcccaaactc aaatatataa agcatttgac ttgttctatg ccctaggggg
     6961 cggggggaag ctaagccagc tttttttaac atttaaaatg ttaattccat tttaaatgca
     7021 cagatgtttt tatttcataa gggttt
//
