LOCUS       Exported                7046 bp ds-DNA     circular SYN 26-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 26, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..198
                     /label=SpeI
     misc_feature    824..1881
                     /label=psi plus pack2
     misc_feature    961..966
                     /label=SpeI
     misc_feature    1300..1794
                     /label=gag
     misc_feature    1886..2111
                     /label=psi plus pack 3prime
     misc_feature    1905..1910
                     /label=AgeI
     misc_feature    2024..2043
                     /label=pBMN Fwd Primer
     misc_feature    2113..2120
                     /label=Kozak
     misc_feature    2116..2121
                     /label=NcoI
     CDS             2121..2825
                     /label=mCherry
     misc_feature    2121..2822
                     /label=mCherry
     misc_feature    2551..2556
                     /label=NcoI
     misc_feature    2759..2780
                     /label=mCh Fwd Internal Primer
     misc_feature    2839..2844
                     /label=XhoI
     misc_feature    2848..2853
                     /label=HindIII
     misc_feature    2856..3522
                     /label=front NDP52 V136S
     misc_feature    3133..3138
                     /label=HindIII
     misc_feature    3523..4190
                     /label=back NDP52 C443K
     misc_feature    3588..3593
                     /label=HindIII
     misc_feature    3696..3701
                     /label=XhoI
     misc_feature    4194..4199
                     /label=BamHI
     misc_feature    complement(4263..4281)
                     /label=pBMN Rev Primer
     rep_origin      complement(5142..5770)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(5925..6584)
                     /label=AmpR
     misc_feature    6411..6429
                     /label=Isotherm Amp2
     misc_feature    complement(6411..6429)
                     /label=Isotherm Amp1
ORIGIN
        1 ggtttcaatg tgcatgaatg ctgcaatatt cctgttacca aagctagtat aaataaaaat
       61 agataaacgt ggaaattact tagagtttct gtcattaacg tttccttcct cagttgacaa
      121 cataaatgcg ctgctgagaa gccagtttgc atctgtcagg atcaatttcc cattatgcca
      181 gtcatattaa ttactagtca attagttgat ttttattttt gacatataca tgtgaaagac
      241 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
      301 acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac agctgaatat
      361 gggccaaagc ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      421 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
      481 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
      541 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
      601 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
      661 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
      721 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
      781 tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg
      841 atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa
      901 cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt
      961 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa
     1021 cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga
     1081 cctgagtcca aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga
     1141 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg
     1201 ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt
     1261 tgtctctgtc tgactgtgtt tctgtatttg tctgaaaata tgggcccggg ccagactgtt
     1321 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac
     1381 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc
     1441 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag
     1501 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc
     1561 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct
     1621 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg
     1681 cctcgatcct ccctttatcc agccctcact ccttctctag gcgcccccat atggccatat
     1741 gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga catgacaaga
     1801 gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt ccagcacgaa
     1861 gtctggagac ctctggcggc agcctaccaa gaacaactgg accgaccggt ggtacctcac
     1921 ccttaccgag tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct
     1981 cgctggaaag gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc
     2041 atcgcagctt ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc
     2101 tctagactgc cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     2161 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     2221 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     2281 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     2341 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     2401 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     2461 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     2521 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     2581 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     2641 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     2701 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     2761 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     2821 acaagtccgg actcagatct cgagctcaag cttgggagga gaccatcaaa gatcccccca
     2881 catcagctgt cttgctggat cactgtcatt tctctcaggt catctttaac agtgtggaga
     2941 agttctacat ccctggaggg gacgtcacat gtcattatac cttcacccag catttcatcc
     3001 ctcgtcgaaa ggattggatt ggcatcttta gagtggggtg gaagacaacc cgtgagtatt
     3061 acaccttcat gtgggttact ttgcccattg acctaaacaa caaatcagct aaacagcagg
     3121 aagtccaatt caaagcttac tacctgccca aggatgatga gtattaccag ttctgctatg
     3181 tggatgagga tggtgtggtc cggggagcaa gtattccttt ccaattccgt ccagaaaatg
     3241 aggaagacat cctggttagt accactcagg gagaggtgga agagattgag cagcacaaca
     3301 aggagctttg caaagaaaac caggagctga aggacagctg tatcagcctc cagaagcaga
     3361 actcagacat gcaggctgag ctccaaaaga agcaggagga gctagaaacc ctacagagca
     3421 tcaataagaa gttggaactg aaagtgaaag aacagaagga ctattgggag acagagctgc
     3481 ttcaactgaa agaacaaaac cagaagatgt cctcagaaaa tgagaagatg ggaatcagag
     3541 tggatcagct tcaggcccag ctgtcaactc aagagaaaga aatggagaag cttgttcagg
     3601 gagatcaaga taagacagag cagttagagc agctgaaaaa ggaaaatgac cacctctttc
     3661 tcagtttagc tgaacagagg aaggaccaga agaagctcga gcagacagtg gagcaaatga
     3721 agcagaatga aactactgca atgaagaaac aacaggaatt aatggatgaa aactttgacc
     3781 tgtcaaaaag actgagtgag aacgaaatta tatgtaatgc tctgcagaga cagaaagaga
     3841 gattggaagg agaaaatgat cttttgaaga gggagaacag cagattgctc agttacatgg
     3901 gtctggattt taattctttg ccgtatcaag tacctacttc agatgaagga ggcgcaagac
     3961 aaaatccagg acttgcctat ggaaacccat attctggtat ccaagaaagt tcttccccca
     4021 gcccgctctc catcaagaaa tgccctatct gcaaagcaga tgatatttgt gatcacacct
     4081 tggagcaaca gcagatgcag cccctttgtt tcaattgtcc aatttgtgac aagatcttcc
     4141 cagctacaga gaagcagatc tttgaagacc acgtgttcaa acactctctc tgaggatcca
     4201 ccggatctag ataactgatc acgataaaat aaaagatttt atttagtctc cagaaaaagg
     4261 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     4321 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     4381 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     4441 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     4501 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     4561 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     4621 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     4681 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     4741 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     4801 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcattca
     4861 catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc
     4921 atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggcgctct
     4981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5041 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5101 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5341 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5581 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagtttgcgc
     5881 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5941 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6001 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6061 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6121 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6181 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6241 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6301 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6361 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6421 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6481 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     6541 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6601 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6661 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6721 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6781 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgacatta
     6841 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaacagct ttgctcttag
     6901 gagtttccta atacatccca aactcaaata tataaagcat ttgacttgtt ctatgcccta
     6961 gggggcgggg ggaagctaag ccagcttttt ttaacattta aaatgttaat tccattttaa
     7021 atgcacagat gtttttattt cataag
//
