LOCUS       Exported                8423 bp ds-DNA     circular SYN 26-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 26, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    188..193
                     /label=SpeI
     misc_feature    819..1876
                     /label=psi plus pack2
     misc_feature    956..961
                     /label=SpeI
     misc_feature    1295..1789
                     /label=gag
     misc_feature    1881..2106
                     /label=psi plus pack 3prime
     misc_feature    1900..1905
                     /label=AgeI
     misc_feature    2019..2038
                     /label=pBMN Fwd Primer
     misc_feature    2108..2115
                     /label=Kozak
     misc_feature    2111..2116
                     /label=NcoI
     CDS             2116..2820
                     /label=mCherry
     misc_feature    2116..2817
                     /label=mCherry
     misc_feature    2546..2551
                     /label=NcoI
     misc_feature    2754..2775
                     /label=mCh Fwd Internal Primer
     misc_feature    2834..2839
                     /label=XhoI
     misc_feature    2843..2848
                     /label=HindIII
     misc_feature    2851..5562
                     /label=NBR1 truncated
     misc_feature    2895..2900
                     /label=HindIII
     misc_feature    3234..3239
                     /label=BamHI
     misc_feature    3361..3366
                     /label=HindIII
     misc_feature    3385..3390
                     /label=HindIII
     misc_feature    3535..3563
                     /label=NBR1 split0
     misc_feature    3843..3848
                     /label=EcoRI
     misc_feature    3852..3857
                     /label=NcoI
     misc_feature    4233..4259
                     /label=NBR1 split1
     misc_feature    4531..4548
                     /label=NBR1 LIR1 F562
     misc_feature    4805..4833
                     /label=NBR1 split2
     misc_feature    5038..5055
                     /label=NBR1 LIR2 Y731
     misc_feature    5347..5373
                     /label=NBR1 split3
     misc_feature    complement(5545..5590)
                     /label=NBR1 Truncation
     misc_feature    5557..5564
                     /label=Kozak
     misc_feature    5566..5571
                     /label=BamHI
     misc_feature    complement(5635..5653)
                     /label=pBMN Rev Primer
     rep_origin      complement(6514..7142)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(7297..7956)
                     /label=AmpR
     misc_feature    7783..7801
                     /label=Isotherm Amp2
     misc_feature    complement(7783..7801)
                     /label=Isotherm Amp1
ORIGIN
        1 caatgtgcat gaatgctgca atattcctgt taccaaagct agtataaata aaaatagata
       61 aacgtggaaa ttacttagag tttctgtcat taacgtttcc ttcctcagtt gacaacataa
      121 atgcgctgct gagaagccag tttgcatctg tcaggatcaa tttcccatta tgccagtcat
      181 attaattact agtcaattag ttgattttta tttttgacat atacatgtga aagaccccac
      241 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
      301 actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
      361 aaagcggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
      421 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      481 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
      541 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
      601 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
      661 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
      721 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
      781 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
      841 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
      901 ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag
      961 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc
     1021 ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga
     1081 gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg agggatatgt
     1141 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
     1201 ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
     1261 ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga ctgttaccac
     1321 tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc
     1381 ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa
     1441 cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa
     1501 ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga
     1561 agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc
     1621 tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg
     1681 atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc catatgagat
     1741 cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac
     1801 taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg
     1861 gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta
     1921 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg
     1981 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc
     2041 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag
     2101 actgccgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     2161 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     2221 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2281 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     2341 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     2401 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     2461 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     2521 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     2581 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     2641 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     2701 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     2761 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     2821 tccggactca gatctcgagc tcaagcttgg gaaccacagg ttactctaaa tgtgactttt
     2881 aaaaatgaaa ttcaaagctt tctggtttct gatccagaaa atacaacttg ggctgatatc
     2941 gaagctatgg taaaagtttc atttgatctg aatactattc aaataaaata cctggatgag
     3001 gaaaatgaag aggtatccat caacagtcaa ggagaatatg aagaagcgct taagatggca
     3061 gttaaacagg gaaaccaact gcagatgcaa gtccacgaag ggcaccatgt cgttgatgaa
     3121 gccccacccc cagttgtagg agcaaaacga ctagctgcca gggcagggaa gaagccactt
     3181 gcacattact cttcactggt gagagtcttg ggatcagaca tgaagacccc agaggatcct
     3241 gcagtgcagt cgtttccact tgttccatgt gacacagacc agcctcaaga caagccccca
     3301 gactggttca caagctacct ggagacgttc agagaacaag tggttaacga aacggttgag
     3361 aagcttgaac agaaattaca tgaaaagctt gtcctccaga acccatcctt gggttcttgt
     3421 ccctcagaag tctcaatgcc tacttcagaa gaaacattgt ttttgccaga aaaccagttc
     3481 agctggcata ttgcttgcaa caactgccaa agaaggattg ttggtgtccg ctaccagtgt
     3541 agcctatgcc catcctacaa tatctgtgaa gattgtgaag cagggccata tggccatgac
     3601 actaaccacg tcctgctgaa gttgcggaga cctgttgtgg gctcctctga accgttctgt
     3661 cactcaaagt actctactcc tcgtcttcct gctgctctgg aacaagtcag gctccagaaa
     3721 caggttgata agaactttct taaagcagaa aagcaaaggt tgcgagctga gaagaaacaa
     3781 cgtaaagcag aggtcaagga acttaaaaag cagcttaaac tccataggaa aattcacctg
     3841 tggaattcaa tccatggact ccagagcccc aagtctcctt taggccgacc tgagagcttg
     3901 ctccagtcta ataccctgat gctccctttg cagccctgta cctccgttat gccaatgctc
     3961 agtgcagcat ttgtggatga gaatttgcct gatgggactc accttcagcc aggaaccaag
     4021 tttatcaaac actggaggat gaaaaataca ggaaatgtaa agtggagtgc agacacaaag
     4081 ctcaagttca tgtggggaaa cctgactttg gcttccacag aaaagaagga tgttttggtt
     4141 ccctgcctca aggccggcca tgtgggagtt gtatctgtgg agttcattgc cccagccttg
     4201 gagggaacgt atacttccca ttggcgtctt tctcacaaag gccagcaatt tgggcctcgg
     4261 gtctggtgca gtatcatagt agatcctttc ccctccgaag agagccctga taacattgaa
     4321 aagggcatga tcagctcaag caaaactgat gatctcacct gccagcaaga ggaaactttt
     4381 cttctggcta aagaagaaag acagcttggt gaagtgactg agcagacaga agggacagca
     4441 gcctgcatcc cacagaaggc aaaaaatgtt gccagtgaga gggagctcta catcccatct
     4501 gtggatcttc tgactgccca ggacctgctg tcctttgagc tgttggatat aaacattgtt
     4561 caagagttgg agagagtgcc ccacaacacc cctgtggatg tgactccctg catgtctcct
     4621 ctgccacatg acagtccttt aatagagaag ccaggcttgg ggcagataga ggaagagaat
     4681 gaaggggcag gatttaaagc acttcctgat tctatggtgt cagtaaagag gaaggctgag
     4741 aacattgctt ctgtggagga agcagaagaa gacctgagtg ggacccagtt tgtgtgtgag
     4801 acagtaatcc gatcccttac cttggatgct gccccagacc acaaccctcc ttgcagacag
     4861 aagtccttgc agatgacatt tgccttgcct gaaggaccac ttggaaatga gaaggaggag
     4921 attatccata tcgctgagga agaagctgtc atggaggagg aggaggatga ggaggatgag
     4981 gaggaggagg atgagctcaa agatgaagtt caaagtcagt cctctgcttc ctcagaggat
     5041 tacatcatca tcctgcctga gtgctttgat accagccgcc ccctggggga ttctatgtac
     5101 agctctgcgc tctcacagcc aggcctggag cgaggtgctg aaggcaagcc tggggttgag
     5161 gctgggcagg aaccagctga ggctggggaa agactccctg gaggggagaa ccagccacag
     5221 gagcacagca taagtgacat cctcacgacc tcacagactc tggaaacagt gcccctaatc
     5281 ccagaggtag tggagcttcc accgtcactg cccaggagct ctccttgtgt acatcatcat
     5341 ggttccccag gagtggattt accagttacc ataccagaag tttcttcagt ccctgatcag
     5401 atcagaggag agcccagagg ctcatcagga cttgtaaaca gcagacagaa gagctatgac
     5461 cactcaaggc accatcatgg gagcagcatt gctggaggac tggtgaaggg ggctttgtct
     5521 gttgctgcct ctgcatacaa ggccctgttt gctgggccac catgaggatc caccggatct
     5581 agataactga tcacgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     5641 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
     5701 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct
     5761 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     5821 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     5881 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag
     5941 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     6001 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     6061 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     6121 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     6181 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt cacatgcagc
     6241 atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg ccatagttgc
     6301 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggcgct cttccgcttc
     6361 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6421 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6481 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6541 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6601 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6661 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6721 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6781 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6841 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6901 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6961 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7021 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7081 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7141 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7201 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttgc gcaaatcaat
     7261 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7321 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7381 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7441 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7501 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7561 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7621 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7681 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7741 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7801 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7861 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     7921 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7981 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8041 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8101 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8161 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgacat taacctataa
     8221 aaataggcgt atcacgaggc cctttcgtct tcaagaacag ctttgctctt aggagtttcc
     8281 taatacatcc caaactcaaa tatataaagc atttgacttg ttctatgccc tagggggcgg
     8341 ggggaagcta agccagcttt ttttaacatt taaaatgtta attccatttt aaatgcacag
     8401 atgtttttat ttcataaggg ttt
//
