LOCUS       GB_UA_25B2             10106 bp    DNA              UNK 01-JAN-1980
DEFINITION  (GB_UA_25A7,GB_UA_25B1)pDGB3_omega1
ACCESSION   GB_UA_25B2
VERSION     GB_UA_25B2
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(9..253)
                     /ApEinfo_revcolor="#b1ff67"
                     /label="misc_feature"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(261..989)
                     /vntifkey="4"
                     /label="YFP"
     mutation        complement(879)
                     /vntifkey="62"
                     /label="T>C"
     misc_feature    complement(991..1718)
     misc_feature    1731..2091
     mutation        2158
                     /vntifkey="62"
                     /label="C>G"
     mutation        2752
                     /vntifkey="62"
                     /label="G>A"
     CDS             2093..3115
                     /vntifkey="4"
                     /label="HygromicinR"
     misc_feature    3123..3404
     misc_feature    3409..3412
                     /vntifkey="21"
                     /label="GTCA"
     misc_feature    3405..3408
                     /vntifkey="21"
                     /label="CGCT"
     misc_feature    3426..3450
                     /vntifkey="21"
                     /label="RB"
     misc_feature    10064..10089
                     /vntifkey="21"
                     /label="LB"
     CDS             complement(8487..9497)
                     /vntifkey="4"
                     /label="SpmR"
     promoter        complement(9498..9631)
                     /vntifkey="30"
                     /label="Spm\Promoter"
     rep_origin      complement(7915..8195)
                     /vntifkey="33"
                     /label="pBR322\ori"
     misc_feature    complement(7515..7775)
                     /note="bom site from pBR322"
                     /vntifkey="21"
                     /label="pBR322"
     misc_feature    complement(4512..5512)
                     /note="STA region from pVS1 plasmid"
                     /vntifkey="21"
                     /label="STA\region\from\pVS1\plasmid"
     misc_feature    6105..7105
                     /note="pVS1-REP; replication origin from pVS1"
                     /vntifkey="21"
                     /label="pVS1-Rep\ori"
ORIGIN
        1 ggagagcgca tgtctcagac ggtcgatgca ttgcagatga gctgtatctg gaagaggtaa
       61 acccgaaacg cgttttattc ttgttgacat ggagctatta aatcactaga aggcactctt
      121 tgctgcttgg acaaatgaac gtatcttatc gagatcctga acaccatttg tctcaactcc
      181 ggagctgaca tcgacaccaa cgatcttata tccagattcg tcaagctgtt tgatgatttc
      241 agtaacgtta agtaagctca gctcatgact gacttgtaga gctcgtccat gccgagagtg
      301 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      361 ttgctcaggg cggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      421 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      481 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
      541 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      601 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
      661 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
      721 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac
      781 ttcaggccgt agccgaaggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      841 gtgcagatga acttcagggt cagcttgccg taggtggcgt cgccctcgcc ctcgccggac
      901 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
      961 gtgaacagct cctcgccctt gctcaccatt ttaaaggttc ttggatggga agatgaatat
     1021 actgaagatg ggaaaagaaa gagaaaagaa aagagcagct ggtggggaga gcaggaaaat
     1081 atggcaacaa atgttggact gacgcaacga ccttgtcaac cccgccgaca caccgggcgg
     1141 acagacgggg caaagctgcc taccagggac tgagggacct cagcaggtcg agtgcagagc
     1201 accggatggg tcgactgcca gcttgtgttc ccggtctgcg ccgctggcca gctcctgagc
     1261 ggcctttccg gtttcataca ccgggcaaag caggagaggc acgatatttg gacgccctac
     1321 agatgccgga tgggccaatt agggagctta cgcgccgggt actcgctcta cctacttcgg
     1381 agaaggtact atctcgtgaa tcttttacca gatcggaagc aattggactt ctgtacctag
     1441 gttaatggca tgctatttcg ccgacggcta tacacccctg gcttcacatt ctccttcgct
     1501 tactgccggt gattcgatga agctccatat tctccgatga tgcaatagat tcttggtcaa
     1561 cgaggggcac accagccttt ccacttcggg gcggaggggc ggccggtccc ggattaataa
     1621 tcatccactg cacctcagag ccgccagagc tgtctggcgc agtggcgctt attactcagc
     1681 ccttctctct gcgtccgtcc gtctgtccgc atgcgccact ccgtcaggag aactgatatt
     1741 gaaggagcat tttttgggct tggctggagc tagtggaggt caacaatgaa tgcctatttt
     1801 ggtttagtcg tccaggcggt gagcacaaaa tttgtgtcgt ttgacaagat ggttcattta
     1861 ggcaactggt cagatcagcc ccacttgtag cagtagcggc ggcgctcgaa gtgtgactct
     1921 tattagcaga caggaacgag gacattatta tcatctgctg cttggtgcac gataacttgg
     1981 tgcgtttgtc aagcaaggta agtggacgac ccggtcatac cttcttaagt tcgcccttcc
     2041 tccctttatt tcagattcaa tctgacttac ctattctacc caagcatcca aaatgaaaaa
     2101 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtgtc
     2161 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     2221 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     2281 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     2341 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     2401 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     2461 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     2521 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     2581 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     2641 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     2701 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg caatgttcgg
     2761 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     2821 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cacgactccg
     2881 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     2941 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     3001 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     3061 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatgagc
     3121 ttgcttcatc attccaccat cattccactc aacattcagg ctcctctgcg cacgtaaagt
     3181 gccaaaggca ataccctgct cggtggaatg ccgccgggct tgtcgatttt acgcacatat
     3241 gcgcattctt gacttgaagc ggaggagttc ttcgttgcgg gttacagtgt tttaataaaa
     3301 gaatggtcaa atcaaactgc tagatatacc tgtcagacac tctagttgtt gacccctata
     3361 ctcttaatac atcagacagt acatgcatgt tgcatgatga cgctcgctgt catgagaccg
     3421 gatcctgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     3481 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     3541 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     3601 gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca caagtcctaa
     3661 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     3721 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     3781 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     3841 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     3901 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     3961 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     4021 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     4081 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     4141 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     4201 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     4261 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     4321 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     4381 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     4441 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     4501 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg
     4561 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     4621 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     4681 acagcttgcg tcatgcggtc gctgcgtata tgatccgatg agtaaataaa caaatacgca
     4741 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     4801 catcggaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     4861 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     4921 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     4981 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     5041 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     5101 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     5161 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     5221 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     5281 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     5341 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     5401 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     5461 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     5521 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     5581 ctgctatctg aatagatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     5641 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     5701 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt
     5761 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca
     5821 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     5881 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     5941 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     6001 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     6061 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     6121 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     6181 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     6241 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     6301 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     6361 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     6421 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     6481 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     6541 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     6601 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     6661 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     6721 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     6781 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     6841 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     6901 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggact ctttcctgtg gatagcacgt
     6961 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     7021 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     7081 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     7141 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
     7201 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
     7261 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
     7321 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7381 tgacacatgc agctcccggt gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7441 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     7501 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     7561 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     7621 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7681 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7741 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7801 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7861 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7921 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7981 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8041 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8101 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8161 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8221 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8281 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8341 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8401 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8461 ggattttggt catgcattct aggtgattat ttgccgacta ccttggtgat ctcgcctttc
     8521 acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt
     8581 ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc
     8641 attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa
     8701 atgcgggaca acgtaagcac tacatttcgc tcatcaccag cccagtcggg cggcgagttc
     8761 catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag
     8821 agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag
     8881 atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc
     8941 tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg
     9001 tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa
     9061 gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc
     9121 gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac
     9181 aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat
     9241 agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt tgttttaggg
     9301 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     9361 taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa
     9421 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     9481 cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc
     9541 cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggtagc
     9601 agcgaagtcg aggcatttct gtcctggctg gaacagaact tattatttcc ttcctctttt
     9661 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
     9721 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
     9781 ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca
     9841 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
     9901 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
     9961 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg
    10021 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
    10081 gtgtaaacat aacggatccg gtctca
//
