LOCUS       fix                     7046 bp    DNA     linear       06-AUG-2019
FEATURES             Location/Qualifiers
     PCR_primer      6826..6849
                     /note="f.ITR for 3.01.15"
     misc_feature    2962..3404
                     /note="synapsin promoter"
     primer_bind     3274..3294
                     /note="f.hsyn seq for.01.7"
     primer_bind     3150..3166
                     /note="f.synapsin_321_seq_for.01.33"
     primer_bind     complement(3274..3294)
                     /note="r.syn seq.01.52"
     primer_bind     complement(3150..3166)
                     /note="r.syn seq.01.53"
     PCR_primer      complement(3046..3066)
                     /note="r.ITR rev 2.01.18 SYN REV"
     primer_bind     2964..2989
                     /note="f.Synapsin pcr for.32.61"
     PCR_primer      2494..2517
                     /note="r.ITR rev 4.01.20"
     misc_feature    complement(2688..2852)
                     /note="ITR"
     PCR_primer      complement(2939..2961)
                     /note="r.ITR rev 1.01.17 SYN REV"
     PCR_primer      2452..2470
                     /note="r.ITR rev 3.01.19"
     PCR_primer      complement(452..472)
                     /note="f.ITR for 1.01.13"
     misc_feature    complement(23..187)
                     /note="ITR"
     misc_feature    complement(194..236)
                     /note=""
     misc_feature    complement(251..270)
                     /note="pzac2.1 5088f"
     PCR_primer      complement(351..374)
                     /note="f.ITR for 2.01.14"
     misc_feature    3405..3544
                     /note="Intron"
     misc_feature    3563..3568
                     /note="KOZAK"
     misc_feature    3569..4861
                     /note="PSAM L131G Q139L Y217F-GlyR"
     misc_feature    3989..3991
                     /note=""
     primer_bind     3827..3850
                     /note="f.GlyR seq .03.70"
     primer_bind     4089..4105
                     /note="f.GlyR seq .03.52"
     misc_feature    3959..3961
                     /note=""
     misc_feature    3983..3985
                     /note=""
     misc_feature    4217..4219
                     /note=""
     primer_bind     4370..4388
                     /note="f.GlyR seq .03.53"
     primer_bind     complement(4553..4576)
                     /note="r.GlyR seq .03.72"
     misc_feature    4873..5471
                     /note="IRES "
     primer_bind     4668..4681
                     /note="f.GlyR seq .03.54"
     misc_feature    5483..6211
                     /note="EGFP "
     primer_bind     5380..5400
                     /note="f.IRES for.01.8"
     misc_feature    5817..5834
                     /note="f.GFP for 2.02.47"
     misc_feature    6028..6050
                     /note="f.GFP seq.02.100"
     misc_feature    6218..6806
                     /note="wpre"
     primer_bind     complement(6289..6308)
                     /note="r.wpre rev.01.3"
     misc_feature    6145..6166
                     /note="f.GFP C.01.39"
     misc_feature    complement(6392..6411)
                     /note="AAV1621r"
     primer_bind     6465..6483
                     /note="f.wpre seq.01.50"
     PCR_primer      242..258
                     /note="1498 primer"
     PCR_primer      774..797
                     /note="1500 primer"
     PCR_primer      complement(774..797)
                     /note="r.AAV backbone seq.33.1519"
     misc_feature    2508..3017
                     /note="1_19 1_20 1_17 1_18 seq clone 1"
     misc_feature    3332..4875
                     /note="EF seq clone 1"
     misc_feature    51..402
                     /note="1_13 1_14 seq"
     misc_feature    6086..6878
                     /note="1_5 seq clone 1"
     misc_feature    join(6879..7046,1..374)
                     /note="1_15 seq clone 1"
     misc_feature    join(6765..7046,1..208)
                     /note="1_14 seq clone 1"
     source          1..7046
                     /dnas_title="fix flex map forward"
ORIGIN      
        1 GGatcttcct agagcatggc tacgtaGATA AGTAGCATGG CGGGTTAATC ATTAACTACA
       61 AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG
      121 CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC
      181 GAGCGCGCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
      241 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
      301 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
      361 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
      421 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
      481 GAGSTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
      541 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
      601 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
      661 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
      721 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
      781 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
      841 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
      901 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
      961 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
     1021 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     1081 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     1141 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     1201 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     1261 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
     1321 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     1381 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     1441 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     1501 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     1561 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     1621 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     1681 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     1741 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     1801 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     1861 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     1921 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     1981 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     2041 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     2101 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTAAA
     2161 TTGTAAGCGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT
     2221 TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG
     2281 GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG
     2341 TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT
     2401 CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC
     2461 GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA
     2521 AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC
     2581 CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG CTGCGCAACT
     2641 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGCGC GCTCGCTCGC
     2701 TCACTGAGGC CGCCCGGGCA AAGCCCGGGC GTCGGGCGAC CTTTGGTCGC CCGGCCTCAG
     2761 TGAGCGAGCG AGCGCGCAGA GAGGGAGTGG CCAACTCCAT CACTAGGGGT TCCTTGTAGT
     2821 TAATGATTAA CCCGCCATGC TACTTATCTA CGTAGCCATG CTCTAGGAAG ATCCGAGCTC
     2881 CAGTGTGCTG GAATTcGCCC TTTTCAGTAT TTAAATTGAC GACCGACCCC GACCCACTgg
     2941 acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg gggaggggaa
     3001 acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca gtgccttcgc
     3061 ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg acgtcactcg
     3121 ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc gccgccggcc
     3181 cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac catctgcgct
     3241 gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt
     3301 gcctgagagc gcagctgtgc tcctgggcac cgcgcagtcc gcccccgcgg ctcctggcca
     3361 gaccacccct aggaccccct gccccaagtc gcagccgttG GATCAggtaa gtatcaaggt
     3421 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt
     3481 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg
     3541 tgATGATATC actagtGCTA GCgccaccAT GCGCTGTTCT CCAGGCGGCG TGTGGCTCGC
     3601 CCTGGCTGCT TCCCTTCTGC ACGTTAGCCT GCAGGGTGAG TTCCAGCGCA AACTGTATAA
     3661 GGAGCTTGTT AAGAATTATA ACCCCCTGGA GCGGCCGGTC GCAAATGATT CCCAGCCACT
     3721 GACAGTGTAC TTCAGCCTCT CCTTGCTGCA GATCATGGAC GTGGATGAAA AGAACCAGGT
     3781 GCTGACCACT AATATTTGGT TGCAGATGTC CTGGACCGAT CACTACTTGC AGTGGAATGT
     3841 GAGCGAATAC CCAGGTGTAA AGACTGTAAG ATTCCCTGAC GGCCAAATCT GGAAACCAGA
     3901 TATCCTGCTG TACAACAGCG CAGACGAAAG GTTTGATGCA ACATTTCACA CCAACGTGgg
     3961 aGTCAATTCT TCAGGCCACT GCCtGTACCT GCCCCCTGGA ATCTTCAAGT CCTCATGCTA
     4021 TATCGACGTC CGCTGGTTTC CCTTCGACGT CCAGCACTGC AAACTCAAAT TCGGGAGCTG
     4081 GAGCTACGGC GGATGGAGCC TGGATCTGCA AATGCAGGAG GCTGACATCT CTGGTTACAT
     4141 CCCGAATGGG GAGTGGGACC TTGTGGGAAT CCCCGGTAAA AGAAGCGAGC GATTTTATGA
     4201 ATGCTGCAAA GAGCCCTtCC CAGATGTCAC CTTCACAGTG ACCATGCGGA GACGCATGGG
     4261 TTATTATCTG ATCCAAATGT ATATCCCAAG CTTGCTTATA GTGATTTTGT CATGGATCTC
     4321 CTTCTGGATT AATATGGACG CCGCTCCAGC TAGGGTCGGA CTGGGCATCA CCACAGTGCT
     4381 GACAATGACT ACTCAGAGCT CAGGCAGCCG AGCCAGCTTG CCCAAGGTTT CTTACGTGAA
     4441 GGCCATCGAT ATCTGGATGG CTGTCTGCCT TCTGTTTGTC TTCAGCGCAC TGCTGGAATA
     4501 CGCCGCTGTC AATTTTGTGT CTCGACAGCA TAAAGAGCTG TTGCGGTTCA GAAGAAAACG
     4561 ACGCCACCAC AAAGAGGATG AGGCAGGAGA AGGACGCTTC AACTTTAGCG CCTATGGTAT
     4621 GGGACCTGCT TGCCTCCAGG CTAAAGACGG AATTTCCGTG AAGGGAGCCA ACAATAGCAA
     4681 CACAACCAAC CCACCCCCTG CTCCATCTAA GAGCCCGGAG GAAATGCGCA AACTCTTTAT
     4741 TCAGAGAGCG AAAAAGATCG ACAAAATCTC CCGGATCGGA TTCCCCATGG CTTTCCTGAT
     4801 TTTCAACATG TTTTATTGGA TCATCTACAA GATTGTGCGA AGGGAGGACG TACACAACCA
     4861 GTAAgcggcc gcaattcccc ccgccccccc cccccccccc tcAccctccc ccccccctaa
     4921 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     4981 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     5041 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5101 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5161 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     5221 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     5281 aagagtcaaa tggctctcct aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     5341 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     5401 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     5461 cgatgataat atggccacaa ccatgggaga tccggtgagc aagggcgagg agctgttcac
     5521 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5581 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5641 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     5701 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5761 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5821 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5881 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     5941 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     6001 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     6061 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     6121 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     6181 cactctcggc atggacgagc tgtacaagta aACCGGTaat caacctctgg attacaaaat
     6241 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6301 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6361 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6421 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6481 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6541 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6601 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6661 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6721 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6781 gatctccctt tgggccgcct ccccgcCGCT TCGAGCAGAC ATGATaagat acattgatga
     6841 gtttggacaA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AATTTGTGAT
     6901 GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC
     6961 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG ATGTGGGAGG TTTTTTAAAG CAAGTAAAAC
     7021 CTCTACAAAT GTGGTAAAAT CGATAA
//

